UniProtKB - Q10473 (GALT1_RAT)
Protein
Polypeptide N-acetylgalactosaminyltransferase 1
Gene
Galnt1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7.
Catalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + H+ + UDPEC:2.4.1.41
- L-threonyl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDPEC:2.4.1.41
Cofactori
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 156 | SubstrateBy similarity | 1 | |
Binding sitei | 186 | SubstrateBy similarity | 1 | |
Metal bindingi | 209 | ManganeseBy similarity | 1 | |
Metal bindingi | 211 | ManganeseBy similarity | 1 | |
Binding sitei | 316 | SubstrateBy similarity | 1 | |
Metal bindingi | 344 | ManganeseBy similarity | 1 | |
Binding sitei | 347 | SubstrateBy similarity | 1 | |
Binding sitei | 352 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- manganese ion binding Source: UniProtKB
- polypeptide N-acetylgalactosaminyltransferase activity Source: RGD
GO - Biological processi
- protein O-linked glycosylation Source: UniProtKB
- protein O-linked glycosylation via serine Source: RGD
- protein O-linked glycosylation via threonine Source: RGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Lectin, Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.4.1.41, 5301 |
Reactomei | R-RNO-6811436, COPI-independent Golgi-to-ER retrograde traffic R-RNO-913709, O-linked glycosylation of mucins |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
Names & Taxonomyi
Protein namesi | Recommended name: Polypeptide N-acetylgalactosaminyltransferase 1 (EC:2.4.1.41)Alternative name(s): Polypeptide GalNAc transferase 1 Short name: GalNAc-T1 Short name: pp-GaNTase 1 Protein-UDP acetylgalactosaminyltransferase 1 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 Cleaved into the following chain: |
Gene namesi | Name:Galnt1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620358, Galnt1 |
Subcellular locationi
Golgi apparatus
- Golgi stack membrane By similarity; Single-pass type II membrane protein By similarity
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi cisterna membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 9 – 28 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 29 – 559 | LumenalSequence analysisAdd BLAST | 531 |
Keywords - Cellular componenti
Golgi apparatus, Membrane, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 106 | C → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 212 | C → A: Loss of enzyme activity due to absence of interaction between UDP moiety and UDP-GalNAC. 1 Publication | 1 | |
Mutagenesisi | 214 | C → A: Loss of enzyme activity due to absence of interaction between UDP moiety and UDP-GalNAC. 1 Publication | 1 | |
Mutagenesisi | 235 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 330 | C → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 339 | C → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 408 | C → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 442 | C → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 444 | D → A: Induces a strong decrease in activity; loss of function; when associated with A-484 and A-525. 2 Publications | 1 | |
Mutagenesisi | 455 | G → Q: Induces a decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 457 | F → A: Little or no effect. 1 Publication | 1 | |
Mutagenesisi | 459 | C → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 465 | N → A: Little or no effect. 1 Publication | 1 | |
Mutagenesisi | 466 | Q → A: Induces a decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 468 | F → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 468 | F → W or Y: Little or no effect. 1 Publication | 1 | |
Mutagenesisi | 482 | C → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 484 | D → A: Loss of enzyme activity; when associated with A-444 and A-525. 2 Publications | 1 | |
Mutagenesisi | 497 | C → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 523 | C → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 525 | D → A: Loss of enzyme activity; when associated with A-444 and A-484. 2 Publications | 1 | |
Mutagenesisi | 540 | C → A: Loss of enzyme activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000223390 | 1 – 559 | Polypeptide N-acetylgalactosaminyltransferase 1Add BLAST | 559 | |
ChainiPRO_0000012263 | 41 – 559 | Polypeptide N-acetylgalactosaminyltransferase 1 soluble formAdd BLAST | 519 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 95 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 106 ↔ 339 | PROSITE-ProRule annotation | ||
Disulfide bondi | 330 ↔ 408 | PROSITE-ProRule annotation | ||
Disulfide bondi | 442 ↔ 459 | PROSITE-ProRule annotation | ||
Disulfide bondi | 482 ↔ 497 | PROSITE-ProRule annotation | ||
Disulfide bondi | 523 ↔ 540 | PROSITE-ProRule annotation | ||
Glycosylationi | 552 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q10473 |
PRIDEi | Q10473 |
PTM databases
GlyGeni | Q10473, 2 sites |
Expressioni
Tissue specificityi
Heart, brain, spleen, liver, skeletal muscle and kidney.
Gene expression databases
Bgeei | ENSRNOG00000016207, Expressed in jejunum and 20 other tissues |
Genevisiblei | Q10473, RN |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 429 – 551 | Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST | 123 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 115 – 225 | Catalytic subdomain AAdd BLAST | 111 | |
Regioni | 285 – 347 | Catalytic subdomain BAdd BLAST | 63 |
Domaini
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates.
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000154732 |
HOGENOMi | CLU_013477_0_1_1 |
InParanoidi | Q10473 |
OMAi | VAEVWMC |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q10473 |
TreeFami | TF313267 |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q10473-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRKFAYCKVV LATSLVWVLL DMFLLLYFSE CNKCEEKKER GLPAGDVLEL
60 70 80 90 100
VQKPHEGPGE MGKPVVIPKE DQEKMKEMFK INQFNLMASE MIAFNRSLPD
110 120 130 140 150
VRLEGCKTKV YPDSLPTTSV VIVFHNEAWS TLLRTVHSVI NRSPRHMIEE
160 170 180 190 200
IVLVDDASER DFLKRPLESY VKKLKVPVHV IRMEQRSGLI RARLKGAAVS
210 220 230 240 250
KGQVITFLDA HCECTVGWLE PLLARIKHDR RTVVCPIIDV ISDDTFEYMA
260 270 280 290 300
GSDMTYGGFN WKLNFRWYPV PQREMDRRKG DRTLPVRTPT MAGGLFSIDR
310 320 330 340 350
DYFQEIGTYD AGMDIWGGEN LEISFRIWQC GGTLEIVTCS HVGHVFRKAT
360 370 380 390 400
PYTFPGGTGQ IINKNNRRLA EVWMDEFKNF FYIISPGVTK VDYGDISSRV
410 420 430 440 450
GLRHKLQCKP FSWYLENIYP DSQIPRHYFS LGEIRNVETN QCLDNMARKE
460 470 480 490 500
NEKVGIFNCH GMGGNQVFSY TANKEIRTDD LCLDVSKLNG PVTMLKCHHL
510 520 530 540 550
KGNQLWEYDP VKLTLQHVNS NQCLDKATEE DSQVPSIRDC TGSRSQQWLL
RNVTLPEIF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35890 mRNA Translation: AAC52511.1 |
RefSeqi | NP_077349.1, NM_024373.1 XP_006254533.1, XM_006254471.3 |
Genome annotation databases
Ensembli | ENSRNOT00000022117; ENSRNOP00000022117; ENSRNOG00000016207 |
GeneIDi | 79214 |
KEGGi | rno:79214 |
UCSCi | RGD:620358, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35890 mRNA Translation: AAC52511.1 |
RefSeqi | NP_077349.1, NM_024373.1 XP_006254533.1, XM_006254471.3 |
3D structure databases
SMRi | Q10473 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000022117 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
PTM databases
GlyGeni | Q10473, 2 sites |
Proteomic databases
PaxDbi | Q10473 |
PRIDEi | Q10473 |
Genome annotation databases
Ensembli | ENSRNOT00000022117; ENSRNOP00000022117; ENSRNOG00000016207 |
GeneIDi | 79214 |
KEGGi | rno:79214 |
UCSCi | RGD:620358, rat |
Organism-specific databases
CTDi | 2589 |
RGDi | 620358, Galnt1 |
Phylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000154732 |
HOGENOMi | CLU_013477_0_1_1 |
InParanoidi | Q10473 |
OMAi | VAEVWMC |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q10473 |
TreeFami | TF313267 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.41, 5301 |
Reactomei | R-RNO-6811436, COPI-independent Golgi-to-ER retrograde traffic R-RNO-913709, O-linked glycosylation of mucins |
Miscellaneous databases
PROi | PR:Q10473 |
Gene expression databases
Bgeei | ENSRNOG00000016207, Expressed in jejunum and 20 other tissues |
Genevisiblei | Q10473, RN |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GALT1_RAT | |
Accessioni | Q10473Primary (citable) accession number: Q10473 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families