UniProtKB - Q0ZME3 (NCAP_CVHN5)
Nucleoprotein
N
Functioni
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
UniRule annotationGO - Molecular functioni
- RNA binding Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding, Viral nucleoprotein |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: NucleoproteinUniRule annotationAlternative name(s): Nucleocapsid proteinUniRule annotation Short name: NCUniRule annotation Short name: Protein NUniRule annotation |
Gene namesi | Name:NUniRule annotation ORF Names:7a |
Organismi | Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) |
Taxonomic identifieri | 443241 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Embecovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion UniRule annotation
- Host endoplasmic reticulum-Golgi intermediate compartment UniRule annotation
- Host Golgi apparatus UniRule annotation Note: Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding.UniRule annotation
Keywords - Cellular componenti
Host Golgi apparatus, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000297769 | 1 – 441 | NucleoproteinAdd BLAST | 441 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 158 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 173 | Phosphothreonine; by hostUniRule annotation | 1 | |
Modified residuei | 190 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 388 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 417 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 421 | Phosphothreonine; by hostUniRule annotation | 1 |
Post-translational modificationi
Keywords - PTMi
ADP-ribosylation, PhosphoproteinInteractioni
Subunit structurei
Homooligomer. Both monomeric and oligomeric forms interact with RNA.
Interacts with protein M.
Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 60 – 189 | CoV N NTDPROSITE-ProRule annotationAdd BLAST | 130 | |
Domaini | 257 – 379 | CoV N CTDPROSITE-ProRule annotationAdd BLAST | 123 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 57 | DisorderedSequence analysisAdd BLAST | 57 | |
Regioni | 53 – 193 | RNA-bindingUniRule annotationAdd BLAST | 141 | |
Regioni | 186 – 226 | DisorderedSequence analysisAdd BLAST | 41 | |
Regioni | 264 – 382 | DimerizationUniRule annotationAdd BLAST | 119 | |
Regioni | 380 – 408 | DisorderedSequence analysisAdd BLAST | 29 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 34 | Polar residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 41 – 57 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 189 – 224 | Polar residuesSequence analysisAdd BLAST | 36 |
Sequence similaritiesi
Family and domain databases
CDDi | cd21595, CoV_N-CTD, 1 hit cd21554, CoV_N-NTD, 1 hit |
HAMAPi | MF_04096, BETA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR044344, N_prot_C_CoV IPR044345, N_prot_N_CoV IPR043505, NCAP_bCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSYTPGHHAG SRSSSGNRSG ILKKTSWVDQ SERSHQTYNR GRKPQPKFTV
60 70 80 90 100
STQPQGNPIP HYSWFSGITQ FQKGRDFKFP DGQGVPIAYG IPPSEAKGYW
110 120 130 140 150
YKHNRRSFKT ADGQQKQLLP RWYFYYLGTG PYASSSYGDA HEGIFWVASH
160 170 180 190 200
QADTSIPSDV SARDPTIQEA IPTRFSPGTI LPQGYYVEGS GRSASNSRPG
210 220 230 240 250
SRSQSRGPNN RSLSRSNSNF RHSDSIVKPD MADEIASLVL AKLGKDSKPQ
260 270 280 290 300
QVTKQNAKEI RHKILMKPRQ KRTPNKFCNV QQCFGKRGPL QNFGNSEMLK
310 320 330 340 350
LGTNDPQFPI LAELAPTPGA FFFGSKLELF KRDSDADSPS KDTFELRYSG
360 370 380 390 400
SIRFDSTLPG FETIMKVLKE NLDAYVNSNQ NTVSGSLSPK PQRKRGVKQS
410 420 430 440
PESFDSLNLS ADTQHISNDF TPEDHSLLAT LDDPYVEDSV A
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ339101 Genomic RNA Translation: ABC70723.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ339101 Genomic RNA Translation: ABC70723.1 |
3D structure databases
SMRi | Q0ZME3 |
ModBasei | Search... |
Family and domain databases
CDDi | cd21595, CoV_N-CTD, 1 hit cd21554, CoV_N-NTD, 1 hit |
HAMAPi | MF_04096, BETA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR044344, N_prot_C_CoV IPR044345, N_prot_N_CoV IPR043505, NCAP_bCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NCAP_CVHN5 | |
Accessioni | Q0ZME3Primary (citable) accession number: Q0ZME3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 21, 2007 |
Last sequence update: | July 24, 2007 | |
Last modified: | February 23, 2022 | |
This is version 66 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |