UniProtKB - Q0WRJ2 (TC10A_ARATH)
Protein
3-dehydrosphinganine reductase TSC10A
Gene
TSC10A
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the reduction of 3-ketodihydrosphingosine (KDS) to dihydrosphingosine (DHS). Required for sphingolipid biosynthesis. In plants, sphingolipids seems to play a critical role in mineral ion homeostasis, most likely through their involvement in the ion transport functionalities of membrane systems in the root. Lacks stereospecificity and can also produce L-threo-DHS in addition to D-erythro-DHS.1 Publication
Catalytic activityi
- EC:1.1.1.1021 Publication
: sphingolipid metabolism Pathwayi
This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 175 | SubstrateBy similarity | 1 | |
Active sitei | 188 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Active sitei | 192 | By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 45 – 56 | NADPBy similarityAdd BLAST | 12 |
GO - Molecular functioni
- 3-dehydrosphinganine reductase activity Source: TAIR
GO - Biological processi
- 3-keto-sphinganine metabolic process Source: GO_Central
- sphingolipid biosynthetic process Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid metabolism, Sphingolipid metabolism |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | MetaCyc:AT3G06060-MONOMER |
UniPathwayi | UPA00222 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-dehydrosphinganine reductase TSC10A (EC:1.1.1.102)Alternative name(s): 3-ketodihydrosphingosine reductase Short name: KDS reductase 3-ketosphinganine reductase |
Gene namesi | Name:TSC10A Ordered Locus Names:At3g06060 ORF Names:F24F17.4 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G06060 |
TAIRi | locus:2080379, AT3G06060 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- endoplasmic reticulum membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | LumenalSequence analysis | 7 | |
Transmembranei | 8 – 28 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 29 – 264 | CytoplasmicSequence analysisAdd BLAST | 236 | |
Transmembranei | 265 – 285 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 286 – 288 | LumenalSequence analysis | 3 | |
Transmembranei | 289 – 309 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 310 – 326 | CytoplasmicSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
High frequency of tricotyledons and altered flower morphology. The double mutants tsc10a and tsc10b are not viable.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000430305 | 1 – 326 | 3-dehydrosphinganine reductase TSC10AAdd BLAST | 326 |
Proteomic databases
PaxDbi | Q0WRJ2 |
PRIDEi | Q0WRJ2 |
ProteomicsDBi | 234206 |
PTM databases
iPTMneti | Q0WRJ2 |
Expressioni
Tissue specificityi
Expressed in roots, leaves, stems, flowers and siliques.1 Publication
Gene expression databases
ExpressionAtlasi | Q0WRJ2, baseline and differential |
Genevisiblei | Q0WRJ2, AT |
Family & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1210, Eukaryota |
HOGENOMi | CLU_010194_3_2_1 |
InParanoidi | Q0WRJ2 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q0WRJ2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAISPLFLL FLIPIIPLSL LAILALIVRP RPIKIPIKSR HAFITGGSSG
60 70 80 90 100
IGLALAHRAA SEGARVSILA RSGSKLEEAK KSIQLATGVE VATFSADVRD
110 120 130 140 150
YDAVSKAIDE SGPIDVLIVN QGVFTAKELV KHSPEDVKFT IDVNLVGSFN
160 170 180 190 200
VIKAALPAMK ARKDRGPASI SLVSSQAGQV GVYGYAAYSA SKFGLQGLAQ
210 220 230 240 250
ALQQEVISDD IHVTLIFPPD TNTPGFEEEQ KSRPEVTAII AASSGSMETE
260 270 280 290 300
EVAKKAMDGI KAGNFTVSCN FEGFLLSLAT TGMSPQRSFW LAFLEVITAG
310 320
PIRLIALFFQ WDWYKAIEKW SKTKTK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1I9LMA8 | A0A1I9LMA8_ARATH | NAD(P)-binding Rossmann-fold superf... | TSC10A At3g06060, F24F17.4, F24F17_4 | 343 | Annotation score: |
Sequence cautioni
The sequence AAF66134 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC068073 Genomic DNA Translation: AAF66134.1 Sequence problems. CP002686 Genomic DNA Translation: AEE74338.1 BT003132 mRNA Translation: AAO24564.1 Sequence problems. AK228314 mRNA Translation: BAF00257.1 |
RefSeqi | NP_187257.2, NM_111481.3 |
Genome annotation databases
EnsemblPlantsi | AT3G06060.1; AT3G06060.1; AT3G06060 |
GeneIDi | 819779 |
Gramenei | AT3G06060.1; AT3G06060.1; AT3G06060 |
KEGGi | ath:AT3G06060 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC068073 Genomic DNA Translation: AAF66134.1 Sequence problems. CP002686 Genomic DNA Translation: AEE74338.1 BT003132 mRNA Translation: AAO24564.1 Sequence problems. AK228314 mRNA Translation: BAF00257.1 |
RefSeqi | NP_187257.2, NM_111481.3 |
3D structure databases
SMRi | Q0WRJ2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT3G06060.1 |
PTM databases
iPTMneti | Q0WRJ2 |
Proteomic databases
PaxDbi | Q0WRJ2 |
PRIDEi | Q0WRJ2 |
ProteomicsDBi | 234206 |
Genome annotation databases
EnsemblPlantsi | AT3G06060.1; AT3G06060.1; AT3G06060 |
GeneIDi | 819779 |
Gramenei | AT3G06060.1; AT3G06060.1; AT3G06060 |
KEGGi | ath:AT3G06060 |
Organism-specific databases
Araporti | AT3G06060 |
TAIRi | locus:2080379, AT3G06060 |
Phylogenomic databases
eggNOGi | KOG1210, Eukaryota |
HOGENOMi | CLU_010194_3_2_1 |
InParanoidi | Q0WRJ2 |
Enzyme and pathway databases
UniPathwayi | UPA00222 |
BioCyci | MetaCyc:AT3G06060-MONOMER |
Miscellaneous databases
PROi | PR:Q0WRJ2 |
Gene expression databases
ExpressionAtlasi | Q0WRJ2, baseline and differential |
Genevisiblei | Q0WRJ2, AT |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TC10A_ARATH | |
Accessioni | Q0WRJ2Primary (citable) accession number: Q0WRJ2 Secondary accession number(s): Q9M7S9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 2014 |
Last sequence update: | September 5, 2006 | |
Last modified: | December 2, 2020 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families