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Protein

Zinc finger protein GLI2

Gene

Gli2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:9006072). Functions as transcriptional activator (PubMed:10806483). May also function as transcriptional repressor (PubMed:10433919). Requires STK36 for full transcriptional activator activity (PubMed:10806483). Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element (By similarity). Is involved in the smoothened (SHH) signaling pathway (PubMed:10433919). Required for normal skeleton development (PubMed:9006072).By similarity1 Publication2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri417 – 444C2H2-type 1PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri455 – 477C2H2-type 2; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri483 – 507C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri513 – 538C2H2-type 4PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri544 – 569C2H2-type 5PROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5610787 Hedgehog 'off' state
R-MMU-5632684 Hedgehog 'on' state

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein GLI2Curated
Alternative name(s):
Tax helper proteinBy similarity
Gene namesi
Name:Gli2Imported
Synonyms:ThpBy similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:95728 Gli2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Full embryonic lethality. Homozygous embryos are detected at the expected Mendelian rate up to about 18.5 dpc, but there are no live pups. Mutant embryos present important craniofacial defects, including defects of the medial portion of the frontal and parietal skull bones, absence of upper and/or lower incisors, often combined with a cleft palate. Besides, mutant embryos show defects in the ossification of skeletal bones and shortened limb bones.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004062151 – 1544Zinc finger protein GLI2Add BLAST1544

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei145PhosphoserineBy similarity1
Modified residuei230PhosphoserineBy similarity1
Modified residuei232PhosphoserineBy similarity1
Modified residuei238PhosphoserineBy similarity1
Modified residuei385Phosphoserine; by DYRK2By similarity1
Modified residuei707PhosphoserineCombined sources1
Modified residuei708PhosphothreonineCombined sources1
Modified residuei740N6-acetyllysine; by EP300By similarity1
Modified residuei997Phosphoserine; by DYRK2By similarity1

Post-translational modificationi

Phosphorylated in vitro by ULK3. Phosphorylated by DYRK2; this inhibits GLI2 transcription factor activity and promotes proteasomal degradation of GLI2.By similarity
Acetylation at Lys-740 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ0VGT2
PeptideAtlasiQ0VGT2
PRIDEiQ0VGT2

PTM databases

iPTMnetiQ0VGT2
PhosphoSitePlusiQ0VGT2

Expressioni

Gene expression databases

BgeeiENSMUSG00000048402 Expressed in 311 organ(s), highest expression level in limb bud
ExpressionAtlasiQ0VGT2 baseline and differential
GenevisibleiQ0VGT2 MM

Interactioni

Subunit structurei

Interacts with ZIC1 and ZIC2 (PubMed:11238441). Interacts with STK36 (PubMed:10806483). Interacts with SUFU; this inhibits transcriptional activation mediated by GLI2 (PubMed:10806483, PubMed:23034632). Interacts (via C-terminal internal region) with FOXC1 (via N-terminus); this interaction is direct and increases GLI2 DNA-binding and transcriptional activity through a smoothened (SMO)-independent Hedgehog (Hh) signaling pathway (PubMed:26565916, PubMed:25808752).By similarity5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199943, 12 interactors
ComplexPortaliCPX-149 GLI2-SUFU complex
CORUMiQ0VGT2
DIPiDIP-60772N
ELMiQ0VGT2
IntActiQ0VGT2, 2 interactors
STRINGi10090.ENSMUSP00000054837

Structurei

3D structure databases

ProteinModelPortaliQ0VGT2
SMRiQ0VGT2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi789 – 854Ser-richAdd BLAST66

Domaini

The N-terminal domain confers transcriptional repressor activity, while the C-terminal domain mediates transcriptional activation.1 Publication

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri417 – 444C2H2-type 1PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri455 – 477C2H2-type 2; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri483 – 507C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri513 – 538C2H2-type 4PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri544 – 569C2H2-type 5PROSITE-ProRule annotationAdd BLAST26

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00900000140802
HOGENOMiHOG000290688
HOVERGENiHBG005844
InParanoidiQ0VGT2
KOiK16798
OMAiGQIHMYE
OrthoDBiEOG091G01XS
TreeFamiTF350216

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 5 hits
SUPFAMiSSF57667 SSF57667, 3 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 4 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.iShow all

Q0VGT2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
METSAPAPAL EKKEAKSGLL EDSSFPDPGK KACPLAVAAA VAAHGVPQQL
60 70 80 90 100
LPAFHAPLPI DMRHQEGRYH YDPHSVHSVH GPPTLSGSPV ISDISLIRLS
110 120 130 140 150
PHPAGPGESP FSAHHPYVNP HMEHYLRSVH SSPTLSMISA ARGLSPADVA
160 170 180 190 200
HEHLKERGLF SLAAPGTNPS DYYHQMTLMA SHPTPYGDLL MQSGGAASAP
210 220 230 240 250
HLHDYLNPVD ASRFSSPRVT PRLSRKRALS ISPLSDASLD LQRMIRTSPN
260 270 280 290 300
SLVAYINNSR SSSAASGSYG HLSAGALSPA FTFPHPINPV AYQQILSQQR
310 320 330 340 350
GLGSAFGHTP PLIQPSPTFL AQQPMTLTSI STMPTQLSSS SSNCLNDANQ
360 370 380 390 400
NKQNSESAVS STVNPITIHK RSKVKTEAEG LRPASPLGLT QEQLADLKED
410 420 430 440 450
LDRDDCKQEA EVVIYETNCH WADCTKEYDT QEQLVHHINN EHIHGEKKEF
460 470 480 490 500
VCRWQACTRE QKPFKAQYML VVHMRRHTGE KPHKCTFEGC SKAYSRLENL
510 520 530 540 550
KTHLRSHTGE KPYVCEHEGC NKAFSNASDR AKHQNRTHSN EKPYICKIPG
560 570 580 590 600
CTKRYTDPSS LRKHVKTVHG PDAHVTKKQR NDVHVRAPLL KENGDNEASA
610 620 630 640 650
EPGGRGPEES VEASSTSHTV EDCLHIKAIK TESSGLCQSS PGAQSSCSSE
660 670 680 690 700
PSPLGSAPNN DSGMEMPGTG PGSLGDLTAL ADTCPGADTS ALAAPSTGGL
710 720 730 740 750
QLRKHMSTVH RFEQLKREKL KSLKDSCSWA GPAPHTRNTK LPPLPVNGSV
760 770 780 790 800
LENFNNTGGG GPAGLLPSQR LPELTEVTML SQLQERRDSS TSTMSSAYTV
810 820 830 840 850
SRRSSGISPY FSSRRSSEAS PLGGLRPHNA SSADSYDPIS TDASRRSSEA
860 870 880 890 900
SQCSGGGPGL LNLTPAQQYN LRAKYAAATG GPPPTPLPGL DRVSLRTRLA
910 920 930 940 950
LLDAPERALP GACPHPLGPR RGSDGPTYSH GHGHGYAGAA PAFPHEGPNS
960 970 980 990 1000
STRRASDPVR RPDPLILPRV QRFHSTHNMN PGSLPPCADR RGLHVQSHPS
1010 1020 1030 1040 1050
VDSNLTRNAY SPRPPSINEN VVMEAVAAGV DGPGLECDLG LVEDELVLPD
1060 1070 1080 1090 1100
DVVQYIKAHT GGTLDDGIRQ GYPTEGTGFP ENSKLPSPGL QGHRRLAAAD
1110 1120 1130 1140 1150
SNMGPSAPGL GGCQLSYSPS SNLNKSNMPV QWNEVSSGTV DALPTQVKPP
1160 1170 1180 1190 1200
PFPHSNLAVV QQKPAFGQYP GYNPQSVQSS SGGLDSTQPH LQLRGAPSAS
1210 1220 1230 1240 1250
RGSYTQQPRQ PAAGSQCLGM SAAMSPQASY SQAHPQLSPN IVSGSLNQFS
1260 1270 1280 1290 1300
PSCSNMAAKP SHLGLPQQME VVPNATIMNG HQREHGVPNS SLAAVSQPHP
1310 1320 1330 1340 1350
VLSYPQQDSY QQGSNLLSSH QPGFMESQQN AGFGLMQPRP PLEPNTASRH
1360 1370 1380 1390 1400
RGVRSGQQQL YARTTGQAMV TSANQETAEA MPKGPAGTMV SLAPQPSQDT
1410 1420 1430 1440 1450
GRAQDQNTLY YYGQIHMYEQ NGGCPAVQPQ PPQPQACSDS IQPEPLPSPG
1460 1470 1480 1490 1500
VNQVSSTVDS QLLEPPQIDF DAIMDDGDHS SLFSGALSPT LLHNLSQNSS
1510 1520 1530 1540
RLTTPRNSLT LPSIPAGISN MAVGDMSSML TSLAEESKFL NMMT
Length:1,544
Mass (Da):165,031
Last modified:July 27, 2011 - v2
Checksum:iEA290773B3DE1D6A
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A5AA27A5AA27_MOUSE
GLI-Kruppel family member
Gli2
447Annotation score:
A5A9W5A5A9W5_MOUSE
GLI-Kruppel family member
Gli2
85Annotation score:
A5AA25A5AA25_MOUSE
GLI-Kruppel family member
Gli2
92Annotation score:
F6UUE3F6UUE3_MOUSE
Zinc finger protein GLI2
Gli2
270Annotation score:
A5AA26A5AA26_MOUSE
GLI-Kruppel family member
Gli2
49Annotation score:
A0A1B0GXK7A0A1B0GXK7_MOUSE
Zinc finger protein GLI2
Gli2
244Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71Y → S in AAH85190 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC109271 Genomic DNA No translation available.
AC122287 Genomic DNA No translation available.
AC132589 Genomic DNA No translation available.
BC085190 mRNA Translation: AAH85190.1
CCDSiCCDS35692.1
RefSeqiNP_001074594.1, NM_001081125.1
UniGeneiMm.273292

Genome annotation databases

EnsembliENSMUST00000062483; ENSMUSP00000054837; ENSMUSG00000048402
GeneIDi14633
KEGGimmu:14633
UCSCiuc007cim.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGLI2_MOUSE
AccessioniPrimary (citable) accession number: Q0VGT2
Secondary accession number(s): E9PYJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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