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Protein

Complement C1s subcomponent

Gene

C1S

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4 (By similarity).By similarity

Catalytic activityi

Cleavage of Arg-|-Ala bond in complement component C4 to form C4a and C4b, and Lys(or Arg)-|-Lys bond in complement component C2 to form C2a and C2b: the 'classical' pathway C3 convertase.

Enzyme regulationi

Inhibited by SERPING1.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60CalciumBy similarity1
Metal bindingi68CalciumBy similarity1
Metal bindingi113CalciumBy similarity1
Metal bindingi131CalciumBy similarity1
Metal bindingi132Calcium; via carbonyl oxygenBy similarity1
Metal bindingi134CalciumBy similarity1
Metal bindingi149CalciumBy similarity1
Metal bindingi150Calcium; via carbonyl oxygenBy similarity1
Metal bindingi153Calcium; via carbonyl oxygenBy similarity1
Active sitei475Charge relay systemBy similarity1
Active sitei529Charge relay systemBy similarity1
Active sitei632Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processComplement pathway, Immunity, Innate immunity
LigandCalcium, Metal-binding

Protein family/group databases

MEROPSiS01.193

Names & Taxonomyi

Protein namesi
Recommended name:
Complement C1s subcomponent (EC:3.4.21.42)
Alternative name(s):
C1 esterase
Complement component 1 subcomponent s
Cleaved into the following 2 chains:
Gene namesi
Name:C1S
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
ChainiPRO_000028586716 – 689Complement C1s subcomponentAdd BLAST674
ChainiPRO_000028586816 – 437Complement C1s subcomponent heavy chainBy similarityAdd BLAST422
ChainiPRO_0000285869438 – 689Complement C1s subcomponent light chainBy similarityAdd BLAST252

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi65 ↔ 83By similarity
Disulfide bondi135 ↔ 147By similarity
Disulfide bondi143 ↔ 156By similarity
Modified residuei149(3R)-3-hydroxyasparagineBy similarity1
Disulfide bondi158 ↔ 171By similarity
Glycosylationi174N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi175 ↔ 202By similarity
Disulfide bondi234 ↔ 251By similarity
Disulfide bondi294 ↔ 341By similarity
Disulfide bondi321 ↔ 354By similarity
Disulfide bondi359 ↔ 403By similarity
Disulfide bondi386 ↔ 421By similarity
Glycosylationi406N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi425 ↔ 549Interchain (between heavy and light chains)PROSITE-ProRule annotation
Disulfide bondi595 ↔ 618By similarity
Disulfide bondi628 ↔ 660By similarity

Post-translational modificationi

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation

Proteomic databases

PaxDbiQ0VCX1
PeptideAtlasiQ0VCX1
PRIDEiQ0VCX1

Interactioni

Subunit structurei

C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. Activated C1s is an disulfide-linked heterodimer of a heavy chain and a light chain (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006358

Structurei

3D structure databases

ProteinModelPortaliQ0VCX1
SMRiQ0VCX1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 130CUB 1PROSITE-ProRule annotationAdd BLAST115
Domaini131 – 172EGF-like; calcium-bindingSequence analysisAdd BLAST42
Domaini175 – 290CUB 2PROSITE-ProRule annotationAdd BLAST116
Domaini292 – 356Sushi 1PROSITE-ProRule annotationAdd BLAST65
Domaini357 – 423Sushi 2PROSITE-ProRule annotationAdd BLAST67
Domaini438 – 681Peptidase S1PROSITE-ProRule annotationAdd BLAST244

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
HOGENOMiHOG000237311
HOVERGENiHBG000559
InParanoidiQ0VCX1
KOiK01331

Family and domain databases

CDDicd00033 CCP, 2 hits
cd00041 CUB, 2 hits
cd00190 Tryp_SPc, 1 hit
Gene3Di2.60.120.290, 2 hits
InterProiView protein in InterPro
IPR035708 Complement_C1s_subcomponent
IPR000859 CUB_dom
IPR001881 EGF-like_Ca-bd_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR035914 Sperma_CUB_dom_sf
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
IPR001254 Trypsin_dom
IPR033116 TRYPSIN_SER
PANTHERiPTHR24255:SF18 PTHR24255:SF18, 1 hit
PfamiView protein in Pfam
PF00431 CUB, 2 hits
PF00084 Sushi, 2 hits
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00032 CCP, 2 hits
SM00042 CUB, 2 hits
SM00179 EGF_CA, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF49854 SSF49854, 2 hits
SSF50494 SSF50494, 1 hit
SSF57535 SSF57535, 2 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS01180 CUB, 2 hits
PS01187 EGF_CA, 1 hit
PS50923 SUSHI, 2 hits
PS50240 TRYPSIN_DOM, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0VCX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWCIVLFSLV AWVYAEPTMY GEILSPNYPQ VYPNEVEKSW DIEVPAGYGI
60 70 80 90 100
HLYFTHLDIE LSENCSYDSV QIMSGGHEEG KLCGRRTNKN SNSPVVKEFH
110 120 130 140 150
IPYSKLQVIF RSDFSNEERF TGFAAYYVAE DIDECTAFAD APCSHFCNNF
160 170 180 190 200
LGGYFCSCPP EYFLHEDKKN CGVNCSGNVF TTMTGEVESP NYPSPYPESS
210 220 230 240 250
RCDYQIQLEE GFRVVVTMRR EDFDVEPADS EGHCPDSLLF VAGDQHFGPY
260 270 280 290 300
CGNGFPGPLT IETQSSALNI IFQTDGSEQR KGWKFRYHGD PIPCPKEVTA
310 320 330 340 350
NSFWEPERAK YVFRDVVKIT CVDGFEVVQG SVGSPSFYST CQSNGKWSNS
360 370 380 390 400
KLRCQPVDCG APEPIQHGRV EDPESTLFGS ITRYSCEMPY YSMECEGSEV
410 420 430 440 450
YHCSGNGSWV NKVLGIEPPK CIAVCGTPSE PFRSTQRIFG GSIAKIENFP
460 470 480 490 500
WQVFFSNPWA GGALIDEYWV LTAAHVVEGN DIPVMYVGSS SVVTSQLSNA
510 520 530 540 550
QMLTAERVFI HPGWEVLDPS ITRKNFDNDI ALVRLRDPVK MGPKVAPICL
560 570 580 590 600
PGTSSEYDPP ENVLGLISGW GRTNVKSHVI KLRGAKLPVA PLSKCREMKG
610 620 630 640 650
VNPGIDISSF VFTENMICAG NDKGVDSCDG DSGGAFAVQD PKENKPKFYV
660 670 680
AGLVSWGPQC GTYGIYTRVK NYVDWIRKTM QEYSAPSVD
Length:689
Mass (Da):76,609
Last modified:May 1, 2007 - v2
Checksum:iB10A00EC4B15465C
GO

Sequence cautioni

The sequence AAI19957 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC119956 mRNA Translation: AAI19957.1 Different initiation.
RefSeqiNP_001070018.1, NM_001076550.1
UniGeneiBt.1491

Genome annotation databases

GeneIDi767827
KEGGibta:767827

Similar proteinsi

Entry informationi

Entry nameiC1S_BOVIN
AccessioniPrimary (citable) accession number: Q0VCX1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: November 22, 2017
This is version 89 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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