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Protein

Eukaryotic translation initiation factor 3 subunit J

Gene

EIF3J

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit JUniRule annotation
Short name:
eIF3jUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 1UniRule annotation
eIF-3-alphaUniRule annotation
eIF3 p35UniRule annotation
Gene namesi
Name:EIF3JUniRule annotation
Synonyms:EIF3S1UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002840601 – 256Eukaryotic translation initiation factor 3 subunit JAdd BLAST256

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineUniRule annotationBy similarity1
Modified residuei11PhosphoserineUniRule annotationBy similarity1
Modified residuei18PhosphoserineUniRule annotationBy similarity1
Cross-linki104Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei107PhosphothreonineUniRule annotationBy similarity1
Modified residuei125PhosphoserineUniRule annotationBy similarity1
Modified residuei252PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.UniRule annotation

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ0VCU8
PRIDEiQ0VCU8

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000465

Structurei

3D structure databases

ProteinModelPortaliQ0VCU8
SMRiQ0VCU8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 67Sufficient for interaction with EIF3BUniRule annotationAdd BLAST67
Regioni241 – 256Promotes stable association with the 40S ribosomeUniRule annotationAdd BLAST16

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili68 – 133UniRule annotationAdd BLAST66

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 6Poly-Ala5
Compositional biasi27 – 30Poly-Gly4
Compositional biasi51 – 55Poly-Asp5
Compositional biasi216 – 222Poly-Lys7

Sequence similaritiesi

Belongs to the eIF-3 subunit J family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4813 Eukaryota
ENOG4111U5G LUCA
HOGENOMiHOG000238746
HOVERGENiHBG066230
InParanoidiQ0VCU8
KOiK03245

Family and domain databases

Gene3Di1.10.246.60, 1 hit
HAMAPiMF_03009 eIF3j, 1 hit
InterProiView protein in InterPro
IPR023194 eIF3-like_dom_sf
IPR013906 eIF3j
PANTHERiPTHR21681 PTHR21681, 1 hit
PfamiView protein in Pfam
PF08597 eIF3_subunit, 1 hit

Sequencei

Sequence statusi: Complete.

Q0VCU8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAAAGDSD SWDADTFSVE DPVRKVGGGG TAGGDRWEGE DEDEDVKDNW
60 70 80 90 100
DDDDDEKKEE AEVKPEVKIS EKKKIAEKIK EKERQQKKRQ EEIKKRLEEP
110 120 130 140 150
EEPKVLTPEE QLADKLRLKK LQEESDLELA KETFGVNNTV YGIDAMNPSS
160 170 180 190 200
RDDFTEFGKL LKDKITQYEK SLYYANFLEA LVRDVCISLE IDDLKKITNS
210 220 230 240 250
LTVLCSEKQK QEKQSKAKKK KKGVVPGGGL KATMKDDLAD YGGYDGGYAQ

DYEDFM
Length:256
Mass (Da):28,951
Last modified:September 5, 2006 - v1
Checksum:i787F639B7BB49D14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC119989 mRNA Translation: AAI19990.1
BC149417 mRNA Translation: AAI49418.1
RefSeqiNP_001069611.1, NM_001076143.1
UniGeneiBt.63473

Genome annotation databases

GeneIDi539052
KEGGibta:539052

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC119989 mRNA Translation: AAI19990.1
BC149417 mRNA Translation: AAI49418.1
RefSeqiNP_001069611.1, NM_001076143.1
UniGeneiBt.63473

3D structure databases

ProteinModelPortaliQ0VCU8
SMRiQ0VCU8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000465

Proteomic databases

PaxDbiQ0VCU8
PRIDEiQ0VCU8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi539052
KEGGibta:539052

Organism-specific databases

CTDi8669

Phylogenomic databases

eggNOGiKOG4813 Eukaryota
ENOG4111U5G LUCA
HOGENOMiHOG000238746
HOVERGENiHBG066230
InParanoidiQ0VCU8
KOiK03245

Family and domain databases

Gene3Di1.10.246.60, 1 hit
HAMAPiMF_03009 eIF3j, 1 hit
InterProiView protein in InterPro
IPR023194 eIF3-like_dom_sf
IPR013906 eIF3j
PANTHERiPTHR21681 PTHR21681, 1 hit
PfamiView protein in Pfam
PF08597 eIF3_subunit, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3J_BOVIN
AccessioniPrimary (citable) accession number: Q0VCU8
Secondary accession number(s): A6QPP4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: September 5, 2006
Last modified: October 10, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Translation initiation factors
    List of translation initiation factor entries
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Main funding by: National Institutes of Health

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