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Protein

Deoxyhypusine hydroxylase

Gene

DOHH

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.UniRule annotation1 Publication

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation1 Publication

Cofactori

Fe2+UniRule annotationBy similarityNote: Binds 2 Fe2+ ions per subunit.UniRule annotationBy similarity

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56Iron 1UniRule annotationBy similarity1
Metal bindingi89Iron 2UniRule annotationBy similarity1
Metal bindingi90Iron 2UniRule annotationBy similarity1
Metal bindingi208Iron 2UniRule annotationBy similarity1
Metal bindingi241Iron 1UniRule annotationBy similarity1
Metal bindingi242Iron 1UniRule annotationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processHypusine biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.99.29 908
ReactomeiR-BTA-204626 Hypusine synthesis from eIF5A-lysine
UniPathwayi
UPA00354

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation1 Publication)
Short name:
DOHHUniRule annotation
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
Gene namesi
Name:DOHHUniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Organism-specific databases

VGNCiVGNC:28164 DOHH

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57E → G: Loss of deoxyhypusine monooxygenase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003264901 – 303Deoxyhypusine hydroxylaseAdd BLAST303

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ0VC53
PRIDEiQ0VC53

Expressioni

Gene expression databases

BgeeiENSBTAG00000005272 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006935

Structurei

3D structure databases

ProteinModelPortaliQ0VC53
SMRiQ0VC53
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati23 – 49HEAT-like PBS-type 1Add BLAST27
Repeati54 – 80HEAT-like PBS-type 2Add BLAST27
Repeati87 – 113HEAT-like PBS-type 3Add BLAST27
Repeati175 – 201HEAT-like PBS-type 4Add BLAST27
Repeati206 – 232HEAT-like PBS-type 5Add BLAST27
Repeati239 – 265HEAT-like PBS-type 6Add BLAST27

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0567 Eukaryota
COG1413 LUCA
GeneTreeiENSGT00500000044957
HOGENOMiHOG000248665
HOVERGENiHBG081460
InParanoidiQ0VC53
KOiK06072
OMAiPEFQYAD
OrthoDBiEOG091G0E2R
TreeFamiTF105626

Family and domain databases

Gene3Di1.25.10.10, 2 hits
HAMAPiMF_03101 Deoxyhypusine_hydroxylase, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR027517 Deoxyhypusine_hydroxylase
IPR021133 HEAT_type_2
IPR004155 PBS_lyase_HEAT
SMARTiView protein in SMART
SM00567 EZ_HEAT, 6 hits
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50077 HEAT_REPEAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q0VC53-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVTEQEVEAV GQTLVDPGQP LQARFRALFT LRGLGGPVAI SWISRAFDDD
60 70 80 90 100
SALLKHELAY CLGQMQDRRA IPVLLDVLRD TRQEPMVRHE AGEALGAIGD
110 120 130 140 150
PEVLEILKQY STDPVVEVAE TCQLAVRRLE WLQQHGGESA VRGPYLSVDP
160 170 180 190 200
APPAEERDLG QLREALLDEA RPLFDRYRAM FALRDAGGKE AALALAEGLR
210 220 230 240 250
CGSALFRHEI GYVLGQMQHE AAVPQLAAAL AQPTENPMVR HECAEALGAI
260 270 280 290 300
ARPACLAALR AHVADPERVV RESCEVALDM YEYETGSTFQ YADGLERLRS

PLS
Length:303
Mass (Da):33,261
Last modified:September 5, 2006 - v1
Checksum:i4487BA5BDFC61AB9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti266P → S in ABL86660 (PubMed:17391984).Curated1
Sequence conflicti266P → S in ABL86661 (PubMed:17391984).Curated1
Sequence conflicti266P → S in ABL86662 (PubMed:17391984).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ990881 mRNA Translation: ABL86660.1
DQ990882 mRNA Translation: ABL86661.1
DQ990883 mRNA Translation: ABL86662.1
BC120351 mRNA Translation: AAI20352.1
RefSeqiNP_001069354.1, NM_001075886.1
XP_005209005.1, XM_005208948.2
XP_010805282.1, XM_010806980.1
UniGeneiBt.10480

Genome annotation databases

EnsembliENSBTAT00000006935; ENSBTAP00000006935; ENSBTAG00000005272
GeneIDi526521
KEGGibta:526521

Similar proteinsi

Entry informationi

Entry nameiDOHH_BOVIN
AccessioniPrimary (citable) accession number: Q0VC53
Secondary accession number(s): A5H2K6, A5H2K8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: September 5, 2006
Last modified: September 12, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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