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Protein

RNA-binding protein 15

Gene

Rbm15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as hematopoietic cell homeostasis, alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA (PubMed:29535189). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29535189). Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex (PubMed:29535189). Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist (By similarity). Required for the development of multiple tissues, such as the maintenance of the homeostasis of long-term hematopoietic stem cells and for megakaryocyte (MK) and B-cell differentiation (PubMed:17283045, PubMed:17376872, PubMed:18981216, PubMed:25468569). Regulates megakaryocyte differentiation by regulating alternative splicing of genes important for megakaryocyte differentiation; probably regulates alternative splicing via m6A regulation (By similarity). Required for placental vascular branching morphogenesis and embryonic development of the heart and spleen (PubMed:18981216). Acts as a regulator of thrombopoietin response in hematopoietic stem cells by regulating alternative splicing of MPL (PubMed:25468569). May also function as an mRNA export factor, stimulating export and expression of RTE-containing mRNAs which are present in many retrotransposons that require to be exported prior to splicing (By similarity). High affinity binding of pre-mRNA to RBM15 may allow targeting of the mRNP to the export helicase DBP5 in a manner that is independent of splicing-mediated NXF1 deposition, resulting in export prior to splicing (By similarity). May be implicated in HOX gene regulation (By similarity).By similarity5 Publications

GO - Molecular functioni

  • mRNA binding Source: UniProtKB
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein 151 Publication
Alternative name(s):
One-twenty two protein 11 Publication
RNA-binding motif protein 151 Publication
Gene namesi
Name:Rbm151 PublicationImported
Synonyms:Kiaa42571 Publication, Ott11 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2443205 Rbm15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality around E9.5 (PubMed:17376872, PubMed:18981216). Early embryos show growth retardation and incomplete closure of the notochord, as well as placental defects in the spongiotrophoblast and syncytiotrophoblast layers, resulting in an arrest of vascular branching morphogenesis (PubMed:18981216). Conditional knockout mice lacking Rbm15 within the hematopoietic compartment display a loss of peripheral B-cells due to a block in pro/pre-B differentiation, as well as a myeloid and megakaryocytic expansion in spleen and bone marrow (PubMed:17376872).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004446111 – 962RNA-binding protein 15Add BLAST962

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei108PhosphoserineBy similarity1
Modified residuei178PhosphoserineBy similarity1
Modified residuei207PhosphoserineBy similarity1
Modified residuei209PhosphoserineBy similarity1
Cross-linki245Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei252PhosphoserineBy similarity1
Modified residuei256PhosphoserineBy similarity1
Modified residuei258PhosphoserineBy similarity1
Modified residuei265PhosphotyrosineBy similarity1
Modified residuei291PhosphoserineBy similarity1
Modified residuei293PhosphoserineBy similarity1
Modified residuei364PhosphoserineBy similarity1
Cross-linki405Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki419Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki444Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei449N6-acetyllysineBy similarity1
Modified residuei567PhosphothreonineBy similarity1
Modified residuei577Asymmetric dimethylarginine; alternate; by PRMT1By similarity1
Modified residuei577Omega-N-methylarginine; alternate; by PRMT1By similarity1
Modified residuei621PhosphoserineBy similarity1
Modified residuei655PhosphoserineBy similarity1
Modified residuei670PhosphoserineBy similarity1
Modified residuei674PhosphoserineBy similarity1
Modified residuei701PhosphoserineBy similarity1
Cross-linki745Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei768PhosphoserineBy similarity1
Modified residuei782PhosphoserineBy similarity1
Modified residuei936PhosphoserineBy similarity1

Post-translational modificationi

Methylated at Arg-577 by PRMT1, leading to promote ubiquitination by CNOT4 and subsequent degradation by the proteasome.By similarity
Ubiquitinated by CNOT4 following methylation at Arg-577 by PRMT1.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ0VBL3
PaxDbiQ0VBL3
PeptideAtlasiQ0VBL3
PRIDEiQ0VBL3

Expressioni

Gene expression databases

BgeeiENSMUSG00000048109 Expressed in 252 organ(s), highest expression level in thymus

Interactioni

Subunit structurei

Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM (PubMed:29535189). The MAC subcomplex is composed of METTL3 and METTL14 (PubMed:29535189). The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B) (PubMed:29535189). Also component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3 (By similarity). Interacts with RBPJ (PubMed:17283045). Interacts (via SPOC domain) with SETD1B (By similarity). Interacts with NXF1, the interaction is required to promote mRNA export (By similarity). Interacts with SF3B1 (By similarity).By similarity2 Publications

Protein-protein interaction databases

IntActiQ0VBL3, 3 interactors
MINTiQ0VBL3
STRINGi10090.ENSMUSP00000054424

Structurei

3D structure databases

ProteinModelPortaliQ0VBL3
SMRiQ0VBL3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini169 – 251RRM 1PROSITE-ProRule annotationAdd BLAST83
Domaini373 – 450RRM 2PROSITE-ProRule annotationAdd BLAST78
Domaini454 – 528RRM 3PROSITE-ProRule annotationAdd BLAST75
Domaini778 – 957SPOCPROSITE-ProRule annotationAdd BLAST180

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi60 – 165Gly-richPROSITE-ProRule annotationAdd BLAST106
Compositional biasi64 – 158Ser-richPROSITE-ProRule annotationAdd BLAST95
Compositional biasi284 – 288Poly-GlySequence analysis5
Compositional biasi315 – 323Poly-ProSequence analysis9
Compositional biasi587 – 591Poly-ProSequence analysis5
Compositional biasi615 – 733Arg-richPROSITE-ProRule annotationAdd BLAST119

Sequence similaritiesi

Belongs to the RRM Spen family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0112 Eukaryota
ENOG410XSAC LUCA
GeneTreeiENSGT00530000063730
HOGENOMiHOG000006547
HOVERGENiHBG082873
InParanoidiQ0VBL3
KOiK13190
OMAiVMIIVRV
OrthoDBiEOG091G0615
PhylomeDBiQ0VBL3
TreeFamiTF315637

Family and domain databases

CDDicd12553 RRM1_RBM15, 1 hit
cd12555 RRM2_RBM15, 1 hit
cd12557 RRM3_RBM15, 1 hit
Gene3Di2.40.290.10, 1 hit
3.30.70.330, 3 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR034470 RBM15_RRM1
IPR034472 RBM15_RRM2
IPR034473 RBM15_RRM3
IPR000504 RRM_dom
IPR016194 SPOC-like_C_dom_sf
IPR012921 SPOC_C
IPR010912 SPOC_met
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
PF07744 SPOC, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 3 hits
SUPFAMiSSF100939 SSF100939, 1 hit
SSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 3 hits
PS50917 SPOC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q0VBL3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRSAGREPLP RRSPRWRRAS PLCETSAGWR VSQLRRDDLR RPSTMKGKER
60 70 80 90 100
SPVKPKRSRG GEDSSSRGER SKKLGGSGGS NGSSSGKTDS GGSRRSLHLD
110 120 130 140 150
KSSSRGGSRE YETGGGSSSS RLHSYSSPST KNSSGGGESR SSSRGGGGES
160 170 180 190 200
RSSGAASSAP GGGDGVEYKT LKISELGSQL SDEAVEDGLF HEFKRFGDVS
210 220 230 240 250
VKISHLSGSG SGDERVAFVN FRRPEDARAA KHARGRLVLY DRPLKIEAVY
260 270 280 290 300
VSRRRSRSPL DKDAYAPSSS VVGTSVGSHR HAPGGGGGQR SLSPGGAALG
310 320 330 340 350
YRDYRLQQLA LGRLPPPPPP PLPRELERER DYPFYDRVRP AYSLEPRVGA
360 370 380 390 400
GAGAAPFREV DEISPEDDQR ANRTLFLGNL DITVTENDLR RAFDRFGVIT
410 420 430 440 450
EVDIKRPSRG QTSTYGFLKF ENLDMSHRAK LAMSGKIIIR NPIKIGYGKA
460 470 480 490 500
TPTTRLWVGG LGPWVPLAAL AREFDRFGTI RTIDYRKGDS WAYIQYESLD
510 520 530 540 550
AAHAAWTHMR GFPLGGPDRR LRVDFADTEH RYQQQYLQPL PLTHYELVTD
560 570 580 590 600
TFGHRAPDPL RSARDRTPPL LYRDRDRDLY TDSDWVPPPP PVRERSARAA
610 620 630 640 650
TSAVTAYEPL DSLDRRRDGW SLDRDRGDRD LPSSRDQPRK RRLPEESGGR
660 670 680 690 700
HLDRSPESER PRKQRHCTPS PDRSPELSSN RDRYNSDNDR SSRLLLLERS
710 720 730 740 750
SPVRDRRGSL EKSQSDKRDR KNSASAERDR KHRTAAPTEG KNPLKKEDRS
760 770 780 790 800
DGNAPSASTS SSKQKPPSQK QDGGTAPVAA SSPKLCLAWQ GMLLLKNSNF
810 820 830 840 850
PSNMHLLQGD LQVASSLLVE GSTGGKVAQL KITQRLRLDQ PKLDEVTRRI
860 870 880 890 900
KVAGPNGYAI LLAVPGSSDS RSSSSSATSD TAASTQRPLR NLVSYLKQKQ
910 920 930 940 950
AAGVISLPVG GNKDKENTGV LHAFPPCEFS QQFLDSPAKA LAKSEEDYLV
960
MIIVRAKLVN SG
Length:962
Mass (Da):105,722
Last modified:September 5, 2006 - v1
Checksum:iD740EDBF23279C5D
GO
Isoform 2 (identifier: Q0VBL3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     956-962: AKLVNSG → GAS

Show »
Length:958
Mass (Da):105,267
Checksum:i51B979FDCC4F9D14
GO

Sequence cautioni

The sequence AAH80828 differs from that shown. Contaminating sequence.Curated
The sequence BAD90348 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41R → P in BAD90348 (Ref. 1) Curated1
Sequence conflicti78G → S in BAE40192 (PubMed:16141072).Curated1
Sequence conflicti157S → A in BAE38672 (PubMed:16141072).Curated1
Sequence conflicti619G → S in BAE40192 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_059625956 – 962AKLVNSG → GAS in isoform 2. 7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220162 mRNA Translation: BAD90348.1 Different initiation.
AC132405 Genomic DNA No translation available.
BC028452 mRNA Translation: AAH28452.1
BC051409 mRNA Translation: AAH51409.1
BC057038 mRNA Translation: AAH57038.1
BC080828 mRNA Translation: AAH80828.1 Sequence problems.
BC120590 mRNA Translation: AAI20591.1
BC137741 mRNA Translation: AAI37742.1
AK166271 mRNA Translation: BAE38672.1
AK168242 mRNA Translation: BAE40192.1
CCDSiCCDS38590.1 [Q0VBL3-1]
RefSeqiNP_001039272.1, NM_001045807.1 [Q0VBL3-1]
UniGeneiMm.27966
Mm.472803

Genome annotation databases

EnsembliENSMUST00000061772; ENSMUSP00000054424; ENSMUSG00000048109 [Q0VBL3-1]
GeneIDi229700
KEGGimmu:229700
UCSCiuc008qwz.1 mouse [Q0VBL3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220162 mRNA Translation: BAD90348.1 Different initiation.
AC132405 Genomic DNA No translation available.
BC028452 mRNA Translation: AAH28452.1
BC051409 mRNA Translation: AAH51409.1
BC057038 mRNA Translation: AAH57038.1
BC080828 mRNA Translation: AAH80828.1 Sequence problems.
BC120590 mRNA Translation: AAI20591.1
BC137741 mRNA Translation: AAI37742.1
AK166271 mRNA Translation: BAE38672.1
AK168242 mRNA Translation: BAE40192.1
CCDSiCCDS38590.1 [Q0VBL3-1]
RefSeqiNP_001039272.1, NM_001045807.1 [Q0VBL3-1]
UniGeneiMm.27966
Mm.472803

3D structure databases

ProteinModelPortaliQ0VBL3
SMRiQ0VBL3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ0VBL3, 3 interactors
MINTiQ0VBL3
STRINGi10090.ENSMUSP00000054424

Proteomic databases

MaxQBiQ0VBL3
PaxDbiQ0VBL3
PeptideAtlasiQ0VBL3
PRIDEiQ0VBL3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061772; ENSMUSP00000054424; ENSMUSG00000048109 [Q0VBL3-1]
GeneIDi229700
KEGGimmu:229700
UCSCiuc008qwz.1 mouse [Q0VBL3-1]

Organism-specific databases

CTDi64783
MGIiMGI:2443205 Rbm15
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0112 Eukaryota
ENOG410XSAC LUCA
GeneTreeiENSGT00530000063730
HOGENOMiHOG000006547
HOVERGENiHBG082873
InParanoidiQ0VBL3
KOiK13190
OMAiVMIIVRV
OrthoDBiEOG091G0615
PhylomeDBiQ0VBL3
TreeFamiTF315637

Miscellaneous databases

ChiTaRSiRbm15 mouse
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048109 Expressed in 252 organ(s), highest expression level in thymus

Family and domain databases

CDDicd12553 RRM1_RBM15, 1 hit
cd12555 RRM2_RBM15, 1 hit
cd12557 RRM3_RBM15, 1 hit
Gene3Di2.40.290.10, 1 hit
3.30.70.330, 3 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR034470 RBM15_RRM1
IPR034472 RBM15_RRM2
IPR034473 RBM15_RRM3
IPR000504 RRM_dom
IPR016194 SPOC-like_C_dom_sf
IPR012921 SPOC_C
IPR010912 SPOC_met
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
PF07744 SPOC, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 3 hits
SUPFAMiSSF100939 SSF100939, 1 hit
SSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 3 hits
PS50917 SPOC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRBM15_MOUSE
AccessioniPrimary (citable) accession number: Q0VBL3
Secondary accession number(s): A0PJG5
, Q3THK4, Q3TLX0, Q571M7, Q66JP8, Q6PGG1, Q7TT82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2018
Last sequence update: September 5, 2006
Last modified: November 7, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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