UniProtKB - Q0VBD2 (MCM10_MOUSE)
Protein
Protein MCM10 homolog
Gene
Mcm10
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38 (By similarity).By similarity
GO - Molecular functioni
- DNA replication origin binding Source: GO_Central
- double-stranded DNA binding Source: GO_Central
- enzyme binding Source: BHF-UCL
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- single-stranded DNA binding Source: GO_Central
GO - Biological processi
- cell population proliferation Source: MGI
- cellular response to DNA damage stimulus Source: UniProtKB
- DNA replication initiation Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA replication |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68962, Activation of the pre-replicative complex |
Names & Taxonomyi
Protein namesi | Recommended name: Protein MCM10 homolog |
Gene namesi | Name:Mcm10 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917274, Mcm10 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase (By similarity).By similarity
Nucleus
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
- replication fork protection complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000278321 | 1 – 885 | Protein MCM10 homologAdd BLAST | 885 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 85 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 639 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 656 | PhosphoserineCombined sources | 1 | |
Cross-linki | 772 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 773 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q0VBD2 |
MaxQBi | Q0VBD2 |
PaxDbi | Q0VBD2 |
PeptideAtlasi | Q0VBD2 |
PRIDEi | Q0VBD2 |
PTM databases
iPTMneti | Q0VBD2 |
PhosphoSitePlusi | Q0VBD2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026669, Expressed in zygote and 212 other tissues |
Genevisiblei | Q0VBD2, MM |
Interactioni
Subunit structurei
Self-associates (By similarity).
Interacts with ORC2. May interact with MCM2 and MCM6.
Interacts with the DNA polymerase alpha subunit POLA1.
Interacts with RECQL4; this interaction regulates RECQL4 unwinding activity.
Interacts with WDHD1 (By similarity).
By similarityGO - Molecular functioni
- enzyme binding Source: BHF-UCL
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 213824, 2 interactors |
STRINGi | 10090.ENSMUSP00000027980 |
Miscellaneous databases
RNActi | Q0VBD2, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 153 | N-terminal domainBy similarityAdd BLAST | 153 | |
Regioni | 251 – 405 | OB-fold domainBy similarityAdd BLAST | 155 | |
Regioni | 406 – 431 | Zinc finger-like 1Add BLAST | 26 | |
Regioni | 793 – 812 | Zinc finger-like 2By similarityAdd BLAST | 20 | |
Regioni | 826 – 846 | Zinc finger-like 3By similarityAdd BLAST | 21 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 101 – 139 | Sequence analysisAdd BLAST | 39 |
Domaini
Each zinc finger-like domain binds a zinc ion and is involved in both ssDNA and dsDNA binding, as is the OB-fold domain.By similarity
The N-terminal domain mediates homodimerization.By similarity
Sequence similaritiesi
Belongs to the MCM10 family.Curated
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG3056, Eukaryota |
GeneTreei | ENSGT00390000007134 |
HOGENOMi | CLU_014680_0_0_1 |
InParanoidi | Q0VBD2 |
OMAi | HIERGDL |
OrthoDBi | 758449at2759 |
PhylomeDBi | Q0VBD2 |
TreeFami | TF313330 |
Family and domain databases
InterProi | View protein in InterPro IPR040184, Mcm10 IPR015411, Rep_factor_Mcm10_C IPR015408, Znf_Mcm10/DnaG |
PANTHERi | PTHR13454, PTHR13454, 1 hit |
Pfami | View protein in Pfam PF09332, Mcm10, 1 hit PF09329, zf-primase, 1 hit |
SMARTi | View protein in SMART SM01280, Mcm10, 1 hit |
i Sequence
Sequence statusi: Complete.
Q0VBD2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVEEDDLCL LTSLLEENEA VLPCSSEKDK SLSLGDGDPD EFDELFDADG
60 70 80 90 100
DGESYTEEAG SGEEGKTGNQ EERLATLFGD VEDLTDDEVA TSKVGNSGPP
110 120 130 140 150
PAPSQEKTSE ELQDELKKLQ EQMKSLQEQL KAASIKQPPG TAPLQEPPDS
160 170 180 190 200
SLQPLLKEKR IRRIQESACF SAELDVPTLP KAKRVARKPK TPAESSSRMR
210 220 230 240 250
TPAQPLQVSS SFLEPNHSSS SRSSTPSPQA VPGNKCSRTI RNQNTVSPGN
260 270 280 290 300
SGDRPQQVSQ VSVEAFSGLR LRRPRVSSTE MSRKMAGRKL IRLPQIKEKM
310 320 330 340 350
ATENLEETDW VTFGVILRKV TPQSATSGQT FSIWKLNDLH DLTQCVSLFL
360 370 380 390 400
FGDVHKDLWK TEQGTVIGLL NANPMKPKDG LKEVCLSIDH PQKVLIMGEA
410 420 430 440 450
MDLGACKAKK KNGEPCTQTV NLHDCEYCQY HIQAQYKKLS AKRTDLQSTF
460 470 480 490 500
SGGRIPKKFR KGTSLKERLC QDGFYYGGVS SESFAASRAA AIAPKKKVQT
510 520 530 540 550
TLTNLVVRGT NSIIQETKQK LGIPQKSLSC SEEFRELMAL PTFGARNLQK
560 570 580 590 600
HLARAKASGS SKPAIQSISA SALLKQQKQQ MLEMRKRRSE DIQKRFLQSS
610 620 630 640 650
SEVQSPAVPS SSRQAAAQSP RTGAEFPRLE GTATPRMPKL GRGISEGDDV
660 670 680 690 700
LFFDDSPPPR PKLSAAAEAK KLAAIAKLRA KGQILTKVDP NNTVRKQMDG
710 720 730 740 750
RAMLGVKERV ENSNTVSPEE ELEPARKKRR EQLAYLESEE FQKILKAKSK
760 770 780 790 800
HTDILKEAEA ELQKSYFEPL VKKEQMEEKM RATREVKCRV VTCRTCTYTH
810 820 830 840 850
FKPLETCVSE QHNLHWHDGV KRFFKCPCGN RTISLDKLPN KHCRNCGLYK
860 870 880
WERDGMLKEK TGPKIGGETL LPRGEEHAKF LNSLK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 50 | G → R in AAH94576 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 673 | A → V in BAC27135 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 873 | R → I in AAH94576 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK030780 mRNA Translation: BAC27135.1 AK041406 mRNA Translation: BAC30933.1 AL928662 Genomic DNA No translation available. BC094576 mRNA Translation: AAH94576.1 BC120689 mRNA Translation: AAI20690.1 BC120687 mRNA Translation: AAI20688.1 |
CCDSi | CCDS15662.1 |
RefSeqi | NP_001292188.1, NM_001305259.1 NP_081566.2, NM_027290.3 |
Genome annotation databases
Ensembli | ENSMUST00000027980; ENSMUSP00000027980; ENSMUSG00000026669 ENSMUST00000102985; ENSMUSP00000100050; ENSMUSG00000026669 |
GeneIDi | 70024 |
KEGGi | mmu:70024 |
UCSCi | uc008ifh.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK030780 mRNA Translation: BAC27135.1 AK041406 mRNA Translation: BAC30933.1 AL928662 Genomic DNA No translation available. BC094576 mRNA Translation: AAH94576.1 BC120689 mRNA Translation: AAI20690.1 BC120687 mRNA Translation: AAI20688.1 |
CCDSi | CCDS15662.1 |
RefSeqi | NP_001292188.1, NM_001305259.1 NP_081566.2, NM_027290.3 |
3D structure databases
SMRi | Q0VBD2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 213824, 2 interactors |
STRINGi | 10090.ENSMUSP00000027980 |
PTM databases
iPTMneti | Q0VBD2 |
PhosphoSitePlusi | Q0VBD2 |
Proteomic databases
EPDi | Q0VBD2 |
MaxQBi | Q0VBD2 |
PaxDbi | Q0VBD2 |
PeptideAtlasi | Q0VBD2 |
PRIDEi | Q0VBD2 |
Protocols and materials databases
Antibodypediai | 11355, 141 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000027980; ENSMUSP00000027980; ENSMUSG00000026669 ENSMUST00000102985; ENSMUSP00000100050; ENSMUSG00000026669 |
GeneIDi | 70024 |
KEGGi | mmu:70024 |
UCSCi | uc008ifh.2, mouse |
Organism-specific databases
CTDi | 55388 |
MGIi | MGI:1917274, Mcm10 |
Phylogenomic databases
eggNOGi | KOG3056, Eukaryota |
GeneTreei | ENSGT00390000007134 |
HOGENOMi | CLU_014680_0_0_1 |
InParanoidi | Q0VBD2 |
OMAi | HIERGDL |
OrthoDBi | 758449at2759 |
PhylomeDBi | Q0VBD2 |
TreeFami | TF313330 |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68962, Activation of the pre-replicative complex |
Miscellaneous databases
BioGRID-ORCSi | 70024, 4 hits in 17 CRISPR screens |
PROi | PR:Q0VBD2 |
RNActi | Q0VBD2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026669, Expressed in zygote and 212 other tissues |
Genevisiblei | Q0VBD2, MM |
Family and domain databases
InterProi | View protein in InterPro IPR040184, Mcm10 IPR015411, Rep_factor_Mcm10_C IPR015408, Znf_Mcm10/DnaG |
PANTHERi | PTHR13454, PTHR13454, 1 hit |
Pfami | View protein in Pfam PF09332, Mcm10, 1 hit PF09329, zf-primase, 1 hit |
SMARTi | View protein in SMART SM01280, Mcm10, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM10_MOUSE | |
Accessioni | Q0VBD2Primary (citable) accession number: Q0VBD2 Secondary accession number(s): Q505F3, Q8BMJ1, Q8BYA7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | September 5, 2006 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families