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Protein

5-methylcytosine rRNA methyltransferase NSUN4

Gene

NSUN4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei237S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei255S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei310NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

  • methyltransferase activity Source: GO_Central
  • small ribosomal subunit rRNA binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, rRNA-binding, Transferase
Biological processRibosome biogenesis, rRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
5-methylcytosine rRNA methyltransferase NSUN4 (EC:2.1.1.-)
Alternative name(s):
NOL1/NOP2/Sun domain family member 4
Gene namesi
Name:NSUN4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Organism-specific databases

VGNCiVGNC:55638 NSUN4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000289233? – 3845-methylcytosine rRNA methyltransferase NSUN4
Transit peptidei1 – ?MitochondrionBy similarity

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei206PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ0V8R7
PRIDEiQ0V8R7

Expressioni

Gene expression databases

BgeeiENSBTAG00000015891 Expressed in 10 organ(s), highest expression level in testis

Interactioni

Subunit structurei

Heterodimer with MTERF4/MTERFD2; this interaction may be required for NSUN4 recruitment to the mitochondrial large ribosomal subunit.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021120

Structurei

3D structure databases

ProteinModelPortaliQ0V8R7
SMRiQ0V8R7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 187S-adenosyl-L-methionine bindingPROSITE-ProRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2198 Eukaryota
COG0144 LUCA
GeneTreeiENSGT00660000095341
HOGENOMiHOG000017426
HOVERGENiHBG106892
InParanoidiQ0V8R7
KOiK21970
OMAiCKMCRLT
OrthoDBiEOG091G07PM
TreeFamiTF321304

Family and domain databases

InterProiView protein in InterPro
IPR001678 MeTrfase_RsmB/NOP2
IPR023267 RCMT
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51686 SAM_MT_RSMB_NOP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0V8R7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAALVVRGVR DMLKRADFAT VPRRQRHKKK WASTEPKFPA TRLALQNFDM
60 70 80 90 100
TYSVQFGDLW PSIRVSLLSE QKYGALVNNF AAGDRVSAEL EQLKARDFVN
110 120 130 140 150
EAVFHREPEP ENSQTAAPSP ASWACSPNLR CFTFTRGDVS RFPPARLGSL
160 170 180 190 200
GLMDYYLMDA ASLLPVLALG LQPGDTVLDL CAAPGGKTLA LLQTGCCRNL
210 220 230 240 250
AANDLSTSRT SRLQRVLHSY VPQDVRDKNR VRVTSWDGRK WGELEGDTYD
260 270 280 290 300
RVLVDVPCTT DRHSLHEEEN SIFQRSRKKE RQMLPVLQVQ LLAAGLLATK
310 320 330 340 350
PGGHIVYSTC SLSHLQNEYV VQGAIELLDN QYSIKVQVED LTHFRKLFMN
360 370 380
TFSFFPSCQV GELVIPNLMA NFGPMYFCKM CRMT
Length:384
Mass (Da):43,199
Last modified:May 29, 2007 - v2
Checksum:iC457DAD9CDED2BD3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12 – 13ML → IP in DY454541 (PubMed:16305752).Curated2
Sequence conflicti43L → P in DY454541 (PubMed:16305752).Curated1
Sequence conflicti167L → P in DY454541 (PubMed:16305752).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT026151 mRNA Translation: ABG66990.1
DY454541 mRNA No translation available.
RefSeqiNP_001178310.1, NM_001191381.1
UniGeneiBt.36843

Genome annotation databases

EnsembliENSBTAT00000021120; ENSBTAP00000021120; ENSBTAG00000015891
GeneIDi618779
KEGGibta:618779

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT026151 mRNA Translation: ABG66990.1
DY454541 mRNA No translation available.
RefSeqiNP_001178310.1, NM_001191381.1
UniGeneiBt.36843

3D structure databases

ProteinModelPortaliQ0V8R7
SMRiQ0V8R7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021120

Proteomic databases

PaxDbiQ0V8R7
PRIDEiQ0V8R7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000021120; ENSBTAP00000021120; ENSBTAG00000015891
GeneIDi618779
KEGGibta:618779

Organism-specific databases

CTDi387338
VGNCiVGNC:55638 NSUN4

Phylogenomic databases

eggNOGiKOG2198 Eukaryota
COG0144 LUCA
GeneTreeiENSGT00660000095341
HOGENOMiHOG000017426
HOVERGENiHBG106892
InParanoidiQ0V8R7
KOiK21970
OMAiCKMCRLT
OrthoDBiEOG091G07PM
TreeFamiTF321304

Gene expression databases

BgeeiENSBTAG00000015891 Expressed in 10 organ(s), highest expression level in testis

Family and domain databases

InterProiView protein in InterPro
IPR001678 MeTrfase_RsmB/NOP2
IPR023267 RCMT
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51686 SAM_MT_RSMB_NOP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNSUN4_BOVIN
AccessioniPrimary (citable) accession number: Q0V8R7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: November 7, 2018
This is version 66 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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