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UniProtKB - Q0S7V5 (FAD3_RHOJR)
Protein
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl:CoA ligase
Gene
fadD3
Organism
Rhodococcus jostii (strain RHA1)
Status
Functioni
Involved in the catabolism of the rings C and D of cholesterol. Catalyzes the ATP-dependent CoA thioesterification of 3aalpha-H-4alpha(3'-propanoate)-7abeta-methylhexahydro-1,5-indanedione (HIP).
1 PublicationCatalytic activityi
- 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + ATP + CoA = 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA + AMP + diphosphate1 PublicationEC:6.2.1.411 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 398 | ATPBy similarity | 1 | |
Binding sitei | 413 | ATPBy similarity | 1 | |
Binding sitei | 504 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 185 – 193 | ATPBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ligase activity Source: UniProtKB-KW
GO - Biological processi
- cholesterol metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl:CoA ligase1 Publication (EC:6.2.1.411 Publication)Short name: HIP:CoA ligase1 Publication |
Gene namesi | Name:fadD31 Publication Ordered Locus Names:RHA1_ro04595Imported |
Organismi | Rhodococcus jostii (strain RHA1) |
Taxonomic identifieri | 101510 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Nocardiaceae › Rhodococcus › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene completely degrade cholesterol to half the yield of wild-type and accumulate HIP.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000430641 | 1 – 515 | 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl:CoA ligaseAdd BLAST | 515 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Phylogenomic databases
eggNOGi | COG0318, Bacteria |
HOGENOMi | CLU_000022_59_7_11 |
OMAi | YIMPKFD |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
Q0S7V5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEQPTTTPS ALKRAAREFG ELTAVADGDV RLTFTQLHDR VRDFAAALSS
60 70 80 90 100
QDVRPGDHVA VWSPNTYHWV VAALGIHYAG ATLVPINTRY TATEALDILE
110 120 130 140 150
RTKTTALVVA GNFLGTDRYA SLRDESSTFD LPTVVRVPVD GGDAELPGVF
160 170 180 190 200
DFDDFLALAD EDTRAEADAR AAAVSPDDVS DVMFTSGTTG RSKGVMSAHR
210 220 230 240 250
QSVGIAQAWG ECAEVTSDDN YLIINPFFHT FGYKAGFLVC LLNGATVVPM
260 270 280 290 300
AVFDVPKVMA TVHDEQITVL PGAPTIFQSI LDHPDRPKYD LSSLRVAITG
310 320 330 340 350
AAAVPVALVE RMQSELSFDA VLTAYGQTEA VVVTMCRTDD DPVTVSTTSG
360 370 380 390 400
RAIPGMEVRI GDQGEILVRG ENVMLGYLDD PESTAKTIDA DGWLHTGDVG
410 420 430 440 450
TLDDRGYVDI TDRLKDMYIS GGFNVYPAEV ENALARLDGV AESAVIGVPD
460 470 480 490 500
ERMGEVGRAY VVAKPGVTLA EDDVVAFCKE RLANFKVPRS VRFVDSLPRN
510
PSGKVMKNVL REEKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000431 Genomic DNA Translation: ABG96381.1 |
RefSeqi | WP_011596962.1, NC_008268.1 |
Genome annotation databases
EnsemblBacteriai | ABG96381; ABG96381; RHA1_ro04595 |
KEGGi | rha:RHA1_ro04595 |
PATRICi | fig|101510.16.peg.4634 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000431 Genomic DNA Translation: ABG96381.1 |
RefSeqi | WP_011596962.1, NC_008268.1 |
3D structure databases
SMRi | Q0S7V5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 101510.RHA1_ro04595 |
Genome annotation databases
EnsemblBacteriai | ABG96381; ABG96381; RHA1_ro04595 |
KEGGi | rha:RHA1_ro04595 |
PATRICi | fig|101510.16.peg.4634 |
Phylogenomic databases
eggNOGi | COG0318, Bacteria |
HOGENOMi | CLU_000022_59_7_11 |
OMAi | YIMPKFD |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FAD3_RHOJR | |
Accessioni | Q0S7V5Primary (citable) accession number: Q0S7V5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 29, 2014 |
Last sequence update: | September 5, 2006 | |
Last modified: | February 23, 2022 | |
This is version 96 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families