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Protein

Phosphoribosylformylglycinamidine synthase subunit PurQ

Gene

purQ

Organism
Rhodococcus jostii (strain RHA1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurL (purL), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (purS)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87NucleophileUniRule annotation1
Active sitei196UniRule annotation1
Active sitei198UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ligase
Biological processPurine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00128

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurQUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IUniRule annotation
Short name:
FGAR amidotransferase IUniRule annotation
Short name:
FGAR-AT IUniRule annotation
Glutaminase PurQUniRule annotation (EC:3.5.1.2UniRule annotation)
Phosphoribosylformylglycinamidine synthase subunit IUniRule annotation
Gene namesi
Name:purQUniRule annotation
Ordered Locus Names:RHA1_ro04827
OrganismiRhodococcus jostii (strain RHA1)
Taxonomic identifieri101510 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus
Proteomesi
  • UP000008710 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002527231 – 225Phosphoribosylformylglycinamidine synthase subunit PurQAdd BLAST225

Proteomic databases

PRIDEiQ0S774

Interactioni

Subunit structurei

Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi101510.RHA1_ro04827

Structurei

3D structure databases

ProteinModelPortaliQ0S774
SMRiQ0S774
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 225Glutamine amidotransferase type-1UniRule annotationAdd BLAST222

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105D21 Bacteria
COG0047 LUCA
HOGENOMiHOG000238240
KOiK01952
OMAiNCDRDVA
OrthoDBiPOG091H0A3G

Family and domain databases

Gene3Di3.40.50.880, 1 hit
HAMAPiMF_00421 PurQ, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR010075 PRibForGlyAmidine_synth_PurQ
PIRSFiPIRSF001586 FGAM_synth_I, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR01737 FGAM_synth_I, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q0S774-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSARVGVITF PGTLDDVDAA RAVTLAGGEA VSLWHGDADL KGVDAVIVPG
60 70 80 90 100
GFSYGDYLRC GAIARFAPVM GKVVQAAQGG MPVLGICNGF QVLCEAGLLP
110 120 130 140 150
GALTRNEGLH FICRDEWLKV EATSTAWTSR YESGAEILVP LKSGEGRYQA
160 170 180 190 200
SENVLDELEG EGRVVFRYVG DNPNGSQRGI AGISSANGRV VGLMPHPEHA
210 220
TEALTGPSDD GLGMFYSVLD SVISA
Length:225
Mass (Da):23,500
Last modified:September 5, 2006 - v1
Checksum:iE0657F2C05EC05D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA Translation: ABG96612.1
RefSeqiWP_009477892.1, NC_008268.1

Genome annotation databases

EnsemblBacteriaiABG96612; ABG96612; RHA1_ro04827
GeneIDi4222378
KEGGirha:RHA1_ro04827

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA Translation: ABG96612.1
RefSeqiWP_009477892.1, NC_008268.1

3D structure databases

ProteinModelPortaliQ0S774
SMRiQ0S774
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi101510.RHA1_ro04827

Proteomic databases

PRIDEiQ0S774

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG96612; ABG96612; RHA1_ro04827
GeneIDi4222378
KEGGirha:RHA1_ro04827

Phylogenomic databases

eggNOGiENOG4105D21 Bacteria
COG0047 LUCA
HOGENOMiHOG000238240
KOiK01952
OMAiNCDRDVA
OrthoDBiPOG091H0A3G

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00128

Family and domain databases

Gene3Di3.40.50.880, 1 hit
HAMAPiMF_00421 PurQ, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR010075 PRibForGlyAmidine_synth_PurQ
PIRSFiPIRSF001586 FGAM_synth_I, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR01737 FGAM_synth_I, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPURQ_RHOJR
AccessioniPrimary (citable) accession number: Q0S774
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: September 5, 2006
Last modified: November 7, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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