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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Rhodococcus jostii (strain RHA1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurS (purS), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurL (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei58UniRule annotation1
Binding sitei61ATPUniRule annotation1
Binding sitei105ATPUniRule annotation1
Metal bindingi107Magnesium 1UniRule annotation1
Active sitei109Proton acceptorUniRule annotation1
Binding sitei130SubstrateUniRule annotation1
Metal bindingi131Magnesium 2UniRule annotation1
Binding sitei259SubstrateUniRule annotation1
Metal bindingi287Magnesium 2UniRule annotation1
Binding sitei519ATPUniRule annotation1
Binding sitei556ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi557Magnesium 1UniRule annotation1
Binding sitei559SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00128

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Short name:
FGAR amidotransferase IIUniRule annotation
Short name:
FGAR-AT IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:RHA1_ro04835
OrganismiRhodococcus jostii (strain RHA1)
Taxonomic identifieri101510 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus
Proteomesi
  • UP000008710 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000503461 – 761Phosphoribosylformylglycinamidine synthase subunit PurLAdd BLAST761

Proteomic databases

PRIDEiQ0S766

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi101510.RHA1_ro04835

Structurei

3D structure databases

ProteinModelPortaliQ0S766
SMRiQ0S766
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 111Substrate bindingUniRule annotation4
Regioni331 – 333Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JIU Bacteria
COG0046 LUCA
HOGENOMiHOG000238227
KOiK01952
OMAiFIEPYQG
OrthoDBiPOG091H009J

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.90.650.10, 4 hits
HAMAPiMF_00420 PurL_2, 1 hit
InterProiView protein in InterPro
IPR010074 PRibForGlyAmidine_synth_PurL
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
PANTHERiPTHR43555 PTHR43555, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PIRSFiPIRSF001587 FGAM_synthase_II, 1 hit
SUPFAMiSSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR01736 FGAM_synth_II, 1 hit

Sequencei

Sequence statusi: Complete.

Q0S766-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSAHPVDTVT AATATPDVAQ PFKELGLKDD EYARIKEILG RRPTEAELAM
60 70 80 90 100
YSVMWSEHCS YKSSKVHLKY FGETTTDEMR SSMLAGIGEN AGVVDIGDGW
110 120 130 140 150
AVTFKVESHN HPSYVEPYQG AATGVGGIVR DIMAMGARPI AVMDQLRFGA
160 170 180 190 200
ADAPDTRRVV DGVVRGVGGY GNSLGLPNVG GETVFDESYA GNPLVNALCA
210 220 230 240 250
GAMRVEDLHL AFASGAGNKI ILFGARTGLD GIGGVSVLAS ETFDGDESGT
260 270 280 290 300
GRKKLPAVQV GDPFTEKVLI ECCLELYAAK LVVGIQDLGG AGLSCATSEL
310 320 330 340 350
AAAGDGGMHI NLEQVPMRAT GMTAAEVLSS ESQERMCAVV TPDNVDAFME
360 370 380 390 400
VCKKWDVLAT VIGEVTDGER LTISWHGETV VDVPPRTVAH EGPVYERPVQ
410 420 430 440 450
RPASQDALVA NTTAGLTRPE SADELKATLL KMLASPALCS RKWITEQYDR
460 470 480 490 500
YVRGNTVLAE NADSGVIRVD EKTGRGIALA TDASGRYTAL DPYAGAQLAL
510 520 530 540 550
AEAFRNVAVT GATPKAVSNC LNFGSPEDPG VMWQFQQAVR GLADGCAKLG
560 570 580 590 600
IPVTGGNVSF YNQTGSTPIL PTPVVAVLGV IDDVHRRIPT GLGLEPGETL
610 620 630 640 650
ILLGDTRDEF DGSIWSQVEH GHLGGVPPKV DLEREQLLAD ILLAASRDGL
660 670 680 690 700
VSAAHDLSEG GLAQTVVEAA LAGETGCRIL LPDDADPFVT LFSESSGRVL
710 720 730 740 750
VAVPRTEETR FTGMCTARNL PWVRIGVVDE GSDSVEVQGQ FSVSLAELRT
760
TFEGTLPALF G
Length:761
Mass (Da):80,289
Last modified:September 5, 2006 - v1
Checksum:iA5FC68D989EAF25A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA Translation: ABG96620.1
RefSeqiWP_009477899.1, NC_008268.1

Genome annotation databases

EnsemblBacteriaiABG96620; ABG96620; RHA1_ro04835
GeneIDi4222386
KEGGirha:RHA1_ro04835

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA Translation: ABG96620.1
RefSeqiWP_009477899.1, NC_008268.1

3D structure databases

ProteinModelPortaliQ0S766
SMRiQ0S766
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi101510.RHA1_ro04835

Proteomic databases

PRIDEiQ0S766

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG96620; ABG96620; RHA1_ro04835
GeneIDi4222386
KEGGirha:RHA1_ro04835

Phylogenomic databases

eggNOGiENOG4108JIU Bacteria
COG0046 LUCA
HOGENOMiHOG000238227
KOiK01952
OMAiFIEPYQG
OrthoDBiPOG091H009J

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00128

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.90.650.10, 4 hits
HAMAPiMF_00420 PurL_2, 1 hit
InterProiView protein in InterPro
IPR010074 PRibForGlyAmidine_synth_PurL
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
PANTHERiPTHR43555 PTHR43555, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PIRSFiPIRSF001587 FGAM_synthase_II, 1 hit
SUPFAMiSSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR01736 FGAM_synth_II, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPURL_RHOJR
AccessioniPrimary (citable) accession number: Q0S766
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: September 12, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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