UniProtKB - Q0S1H1 (DXS_RHOJR)
Protein
1-deoxy-D-xylulose-5-phosphate synthase
Gene
dxs
Organism
Rhodococcus jostii (strain RHA1)
Status
Functioni
Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).UniRule annotation
Catalytic activityi
- D-glyceraldehyde 3-phosphate + H+ + pyruvate = 1-deoxy-D-xylulose 5-phosphate + CO2UniRule annotationEC:2.2.1.7UniRule annotation
Cofactori
Protein has several cofactor binding sites:- Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
- thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation
: 1-deoxy-D-xylulose 5-phosphate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- 1-deoxy-D-xylulose-5-phosphate synthase (dxs)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate, the pathway 1-deoxy-D-xylulose 5-phosphate biosynthesis and in Metabolic intermediate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 73 | Thiamine pyrophosphateUniRule annotation | 1 | |
Metal bindingi | 145 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 175 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 175 | Thiamine pyrophosphateUniRule annotation | 1 | |
Binding sitei | 286 | Thiamine pyrophosphateUniRule annotation | 1 | |
Binding sitei | 367 | Thiamine pyrophosphateUniRule annotation | 1 |
GO - Molecular functioni
- 1-deoxy-D-xylulose-5-phosphate synthase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
- thiamine pyrophosphate binding Source: UniProtKB-UniRule
GO - Biological processi
- 1-deoxy-D-xylulose 5-phosphate biosynthetic process Source: UniProtKB-UniPathway
- terpenoid biosynthetic process Source: UniProtKB-UniRule
- thiamine biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Transferase |
Biological process | Isoprene biosynthesis, Thiamine biosynthesis |
Ligand | Magnesium, Metal-binding, Thiamine pyrophosphate |
Enzyme and pathway databases
BioCyci | RJOS101510:G1G71-6823-MONOMER |
UniPathwayi | UPA00064;UER00091 |
Names & Taxonomyi
Protein namesi | Recommended name: 1-deoxy-D-xylulose-5-phosphate synthaseUniRule annotation (EC:2.2.1.7UniRule annotation)Alternative name(s): 1-deoxyxylulose-5-phosphate synthaseUniRule annotation Short name: DXP synthaseUniRule annotation Short name: DXPSUniRule annotation |
Gene namesi | Name:dxsUniRule annotation Ordered Locus Names:RHA1_ro06843 |
Organismi | Rhodococcus jostii (strain RHA1) |
Taxonomic identifieri | 101510 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Nocardiaceae › Rhodococcus › |
Proteomesi |
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PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000256477 | 1 – 649 | 1-deoxy-D-xylulose-5-phosphate synthaseAdd BLAST | 649 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 101510.RHA1_ro06843 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 114 – 116 | Thiamine pyrophosphate bindingUniRule annotation | 3 | |
Regioni | 146 – 147 | Thiamine pyrophosphate bindingUniRule annotation | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1154, Bacteria |
HOGENOMi | CLU_009227_1_4_11 |
OMAi | QVGYHAQ |
Family and domain databases
CDDi | cd02007, TPP_DXS, 1 hit |
Gene3Di | 3.40.50.920, 1 hit |
HAMAPi | MF_00315, DXP_synth, 1 hit |
InterProi | View protein in InterPro IPR005477, Dxylulose-5-P_synthase IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR020826, Transketolase_BS IPR033248, Transketolase_C IPR005474, Transketolase_N |
PANTHERi | PTHR43322, PTHR43322, 1 hit |
Pfami | View protein in Pfam PF13292, DXP_synthase_N, 1 hit PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
TIGRFAMsi | TIGR00204, dxs, 1 hit |
PROSITEi | View protein in PROSITE PS00801, TRANSKETOLASE_1, 1 hit PS00802, TRANSKETOLASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q0S1H1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGVLARIQTP DDLRQLNSAE MKQLAAEIRE FLVQKVAATG GHLGPNLGVV
60 70 80 90 100
ELTLALHRIF DSPADPIIFD TGHQAYVHKI LTGRKDDFDS LRKQGGLSGY
110 120 130 140 150
PCRAESDHDW VESSHASASL SYADGLAKAF ELTGQDRHVV AVVGDGALTG
160 170 180 190 200
GMCWEALNNI AAGKDRSVVI VVNDNGRSYA PTIGGLADHL AALRLQPGYE
210 220 230 240 250
RILDSGRRMV KKLPWVGRTA YSVLHGMKAG LKDAVAPQVM FTDLGIKYLG
260 270 280 290 300
PVDGHDEAAL ESALRRAKAF GGPVIVHAVT RKGMGYAPAE NHVADQMHST
310 320 330 340 350
GVIDPITGRG TGSASADWTS VFSAELIDQA SHRQDIVAIT AAMAGPTGLA
360 370 380 390 400
AFGEKYPDRM FDVGIAEQHA VTSAAGLALG GLHPVVAVYS TFLNRAFDQL
410 420 430 440 450
LMDVALLKLP VTLVLDRAGI TGNDGASHNG MWDMSLLGIV PGMKVAAPRD
460 470 480 490 500
TATLREELDE ALAVDDGPTA LRFPKGTVGD DVPAVSRLDG VVDILRAPSG
510 520 530 540 550
RNDVLIVSVG AFAGLALAAA ERLEQQGISA TVVDPRWVLP VPESLVKLAE
560 570 580 590 600
DYTMVVTVED SGLHGGVGST VSAALRAAGV DVPCRDLGVP QQFLDHASRA
610 620 630 640
QIHAELGLTA QDVARQITGW FAGLGNLRPE QQNGVVADLD AQRAEKQQG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000431 Genomic DNA Translation: ABG98615.1 |
RefSeqi | WP_009480093.1, NC_008268.1 |
Genome annotation databases
EnsemblBacteriai | ABG98615; ABG98615; RHA1_ro06843 |
KEGGi | rha:RHA1_ro06843 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000431 Genomic DNA Translation: ABG98615.1 |
RefSeqi | WP_009480093.1, NC_008268.1 |
3D structure databases
SMRi | Q0S1H1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 101510.RHA1_ro06843 |
Genome annotation databases
EnsemblBacteriai | ABG98615; ABG98615; RHA1_ro06843 |
KEGGi | rha:RHA1_ro06843 |
Phylogenomic databases
eggNOGi | COG1154, Bacteria |
HOGENOMi | CLU_009227_1_4_11 |
OMAi | QVGYHAQ |
Enzyme and pathway databases
UniPathwayi | UPA00064;UER00091 |
BioCyci | RJOS101510:G1G71-6823-MONOMER |
Family and domain databases
CDDi | cd02007, TPP_DXS, 1 hit |
Gene3Di | 3.40.50.920, 1 hit |
HAMAPi | MF_00315, DXP_synth, 1 hit |
InterProi | View protein in InterPro IPR005477, Dxylulose-5-P_synthase IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR020826, Transketolase_BS IPR033248, Transketolase_C IPR005474, Transketolase_N |
PANTHERi | PTHR43322, PTHR43322, 1 hit |
Pfami | View protein in Pfam PF13292, DXP_synthase_N, 1 hit PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
TIGRFAMsi | TIGR00204, dxs, 1 hit |
PROSITEi | View protein in PROSITE PS00801, TRANSKETOLASE_1, 1 hit PS00802, TRANSKETOLASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DXS_RHOJR | |
Accessioni | Q0S1H1Primary (citable) accession number: Q0S1H1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | September 5, 2006 | |
Last modified: | April 7, 2021 | |
This is version 101 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families