UniProtKB - Q0P4F7 (ACSF2_DANRE)
Protein
Medium-chain acyl-CoA ligase ACSF2, mitochondrial
Gene
acsf2
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA. Has some preference toward medium-chain substrates. Plays a role in adipocyte differentiation.By similarity
Catalytic activityi
- a medium chain fatty acid + ATP + CoA = a medium-chain fatty acyl-CoA + AMP + diphosphateBy similarityEC:6.2.1.2By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 484 | ATPBy similarity | 1 | |
Binding sitei | 499 | ATPBy similarity | 1 | |
Binding sitei | 590 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 256 – 264 | ATPBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- butyrate-CoA ligase activity Source: UniProtKB-EC
- medium-chain fatty acid-CoA ligase activity Source: UniProtKB
GO - Biological processi
- fatty acid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DRE-77289 Mitochondrial Fatty Acid Beta-Oxidation |
Names & Taxonomyi
Protein namesi | Recommended name: Medium-chain acyl-CoA ligase ACSF2, mitochondrialBy similarity (EC:6.2.1.2By similarity) |
Gene namesi | Name:acsf2By similarity ORF Names:zgc:152887 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-060825-7 acsf2 |
Subcellular locationi
Mitochondrion
- Mitochondrion Curated
Mitochondrion
- mitochondrion Source: UniProtKB-SubCell
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 13 | MitochondrionSequence analysisAdd BLAST | 13 | |
ChainiPRO_0000315798 | 14 – 606 | Medium-chain acyl-CoA ligase ACSF2, mitochondrialAdd BLAST | 593 |
Proteomic databases
PaxDbi | Q0P4F7 |
PRIDEi | Q0P4F7 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1177 Eukaryota COG0318 LUCA |
HOGENOMi | HOG000229999 |
InParanoidi | Q0P4F7 |
PhylomeDBi | Q0P4F7 |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110 AMP-bd_C IPR020845 AMP-binding_CS IPR000873 AMP-dep_Synth/Lig IPR042099 AMP-dep_Synthh-like_sf |
Pfami | View protein in Pfam PF00501 AMP-binding, 1 hit PF13193 AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455 AMP_BINDING, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q0P4F7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSKILLTNL RTSASFCKTL KFPQRPRTPF IASQQSCAIH VDNPPSIPTL
60 70 80 90 100
TTSYVHGLSS HPLQSSTVDQ CLQATVERYP DREAMVFVQD GIRKTFAEFY
110 120 130 140 150
QDVEKAAAGL LAAGLKRGDR LGMWGPNIYE WVLMQFATAK AGIILVAVNP
160 170 180 190 200
AYQLQEVEFA LRKVQCNAVV CPTKFKSQHY CDMLKQLCPE METASPGGIK
210 220 230 240 250
SSRLPDLHTV IVTDSQQPGS FLLKDLMQAG SSQHYQQLQD LQKKLVCDDP
260 270 280 290 300
INIQFTSGTT GKPKGATLSH HNIVNNAYFT GMRIGYNWRK NVRICLPVPL
310 320 330 340 350
YHCFGSVGGG VIMALYGTTV IFPSTGYDGR ANLRAIEKEK CTFVYGTPTM
360 370 380 390 400
YIDMLGQPDL AKFDLSSVRG GIAAGSPCPP EVMRKILNVM GIKEMVIGYG
410 420 430 440 450
TTENSPVTFC GFPVDSAERK IVTVGCISPH TEAKVVDPTT GEIVPLGAQG
460 470 480 490 500
ELMIRGYCVM LEYWQDEEKT RECITKDRWY KTGDIASLDQ FAYCKIEGRI
510 520 530 540 550
KDLIIRGGEN IYPAEIEQFL HTHPKILEAQ VVGVKDERMG EEVCACIRLK
560 570 580 590 600
EGQECTVEEI KAYCKGKIAH YKVPRYILFV QDYPLTITGK IQKHKLRERT
EKQLGL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1R1C0 | F1R1C0_DANRE | Acyl-CoA synthetase family member 2... | acsf2 | 606 | Annotation score: | ||
E9QDF8 | E9QDF8_DANRE | Medium-chain acyl-CoA ligase ACSF2,... | acsf2 | 231 | Annotation score: | ||
E9QEB7 | E9QEB7_DANRE | Medium-chain acyl-CoA ligase ACSF2,... | acsf2 | 181 | Annotation score: | ||
E9QHF9 | E9QHF9_DANRE | Medium-chain acyl-CoA ligase ACSF2,... | acsf2 | 135 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC122098 mRNA Translation: AAI22099.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC122098 mRNA Translation: AAI22099.1 |
3D structure databases
SMRi | Q0P4F7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000081474 |
Proteomic databases
PaxDbi | Q0P4F7 |
PRIDEi | Q0P4F7 |
Organism-specific databases
ZFINi | ZDB-GENE-060825-7 acsf2 |
Phylogenomic databases
eggNOGi | KOG1177 Eukaryota COG0318 LUCA |
HOGENOMi | HOG000229999 |
InParanoidi | Q0P4F7 |
PhylomeDBi | Q0P4F7 |
Enzyme and pathway databases
Reactomei | R-DRE-77289 Mitochondrial Fatty Acid Beta-Oxidation |
Miscellaneous databases
PROi | PR:Q0P4F7 |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110 AMP-bd_C IPR020845 AMP-binding_CS IPR000873 AMP-dep_Synth/Lig IPR042099 AMP-dep_Synthh-like_sf |
Pfami | View protein in Pfam PF00501 AMP-binding, 1 hit PF13193 AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455 AMP_BINDING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACSF2_DANRE | |
Accessioni | Q0P4F7Primary (citable) accession number: Q0P4F7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | September 19, 2006 | |
Last modified: | September 18, 2019 | |
This is version 60 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families