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UniProtKB - Q0KJL4 (LIGU_NOVK1)
Protein
(4E)-oxalomesaconate Delta-isomerase
Gene
ligU
Organism
Novosphingobium sp. (strain KA1) (Sphingomonas sp. (strain KA1))
Status
Functioni
Contributes to the degradation of lignin at the level of the protocatechuate 4,5-cleavage pathway. Catalyzes the isomerization of the double bond between C4 and C5 in (4E)-oxalomesaconate (OMA) to (3Z)-2-keto-4-carboxy-3-hexenedioate (KCH), where the double bond has migrated between C3 and C4 via a 1,3-allylic isomerization.
1 PublicationCatalytic activityi
Kineticsi
kcat is 1300 sec(-1) (at pH 8.0).1 Publication
- KM=170 µM for (4E)-oxalomesaconate (at pH 8.0)1 Publication
: lignin degradation Pathwayi
This protein is involved in the pathway lignin degradation, which is part of Secondary metabolite metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway lignin degradation and in Secondary metabolite metabolism.
GO - Molecular functioni
- isomerase activity Source: UniProtKB-KW
GO - Biological processi
- lignin catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Isomerase |
Biological process | Aromatic hydrocarbons catabolism |
Enzyme and pathway databases
SABIO-RKi | Q0KJL4 |
UniPathwayi | UPA00892 |
Names & Taxonomyi
Protein namesi | Recommended name: (4E)-oxalomesaconate Delta-isomerase1 Publication (EC:5.3.3.-1 Publication)Short name: OMA isomerase1 Publication Alternative name(s): 1,3-allylic isomerase LigU1 Publication |
Gene namesi | Name:ligU1 Publication ORF Names:ORF100Imported |
Encoded oni | Plasmid pCAR3Imported |
Organismi | Novosphingobium sp. (strain KA1) (Sphingomonas sp. (strain KA1)) |
Taxonomic identifieri | 164608 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Novosphingobium › unclassified Novosphingobium |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000446300 | 1 – 357 | (4E)-oxalomesaconate Delta-isomeraseAdd BLAST | 357 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q0KJL4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the PrpF family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR007400, PrpF_protein |
PANTHERi | PTHR43709, PTHR43709, 1 hit |
Pfami | View protein in Pfam PF04303, PrpF, 1 hit |
i Sequence
Sequence statusi: Complete.
Q0KJL4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPRRDRNMDS APCMWMRGGT SKGGYFLRAD LPADTAARDA FLLAVMGSPD
60 70 80 90 100
PRQIDGMGGA DPLTSKVAVV SKSERPGIDV DYLFLQVFVD QAIVTDAQNC
110 120 130 140 150
GNILAGVGPF AIERGLVAAS GDETRVAIFM ENTGQVAVAT VRTPGGSVTY
160 170 180 190 200
AGDAAIDGVP GTHAPIPTEF RDTAGSSCGA LLPSGNAVDV VNGLPVTLID
210 220 230 240 250
NGMPCVVMKA ADVGITGYED RDSLDANAEL KAKIEAIRLA VGELMNLGDV
260 270 280 290 300
TEKSVPKMML VAPPRDGGAV CVRSFIPHRA HATIGVLGAV SVATACLIPG
310 320 330 340 350
SPAAEVAVVP EGARKTLSIE HPTGEMSCVL EVDDAGNVVS AALLRTARKL
MDGVVFV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB270530 Genomic DNA Translation: BAF03328.1 |
RefSeqi | WP_011607995.1, NC_008308.1 YP_718040.1, NC_008308.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB270530 Genomic DNA Translation: BAF03328.1 |
RefSeqi | WP_011607995.1, NC_008308.1 YP_718040.1, NC_008308.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6P3H | X-ray | 1.62 | A/B/C/D | 1-357 | [»] | |
6P3J | X-ray | 2.02 | A/B | 1-357 | [»] | |
6P3K | X-ray | 1.88 | A/B | 1-357 | [»] | |
SMRi | Q0KJL4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00892 |
SABIO-RKi | Q0KJL4 |
Family and domain databases
InterProi | View protein in InterPro IPR007400, PrpF_protein |
PANTHERi | PTHR43709, PTHR43709, 1 hit |
Pfami | View protein in Pfam PF04303, PrpF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LIGU_NOVK1 | |
Accessioni | Q0KJL4Primary (citable) accession number: Q0KJL4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 13, 2019 |
Last sequence update: | October 3, 2006 | |
Last modified: | June 2, 2021 | |
This is version 47 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, PlasmidDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families