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Protein

F-BAR domain only protein 2

Gene

FCHO2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP-2-dependent endocytosis of the transferrin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor.4 Publications

Miscellaneous

Deforms liposomes into a range of tubule diameters from 20 to 130 nm in vitro.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis

Enzyme and pathway databases

ReactomeiR-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
F-BAR domain only protein 2
Gene namesi
Name:FCHO2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000157107.13
HGNCiHGNC:25180 FCHO2
MIMi613438 gene
neXtProtiNX_Q0JRZ9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Coated pit, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10F → E: Binds preferentially to larger liposomes. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000157107
PharmGKBiPA134911830

Polymorphism and mutation databases

BioMutaiFCHO2
DMDMi119369487

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002660051 – 810F-BAR domain only protein 2Add BLAST810

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi147Interchain (with C-273)1 Publication
Disulfide bondi273Interchain (with C-147)1 Publication
Cross-linki297Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei312PhosphoserineBy similarity1
Modified residuei385PhosphothreonineCombined sources1
Modified residuei387PhosphoserineCombined sources1
Modified residuei394PhosphoserineCombined sources1
Modified residuei403PhosphoserineCombined sources1
Modified residuei488PhosphoserineCombined sources1
Modified residuei493PhosphoserineBy similarity1
Modified residuei496PhosphoserineCombined sources1
Modified residuei508PhosphoserineCombined sources1
Modified residuei510PhosphoserineBy similarity1
Modified residuei511PhosphoserineCombined sources1
Modified residuei533PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated. Mainly undergoes monoubiquitination but also polyubiquitination (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ0JRZ9
MaxQBiQ0JRZ9
PaxDbiQ0JRZ9
PeptideAtlasiQ0JRZ9
PRIDEiQ0JRZ9
ProteomicsDBi58761
58762 [Q0JRZ9-2]

PTM databases

iPTMnetiQ0JRZ9
PhosphoSitePlusiQ0JRZ9

Expressioni

Gene expression databases

BgeeiENSG00000157107 Expressed in 204 organ(s), highest expression level in mouth mucosa
CleanExiHS_FCHO2
ExpressionAtlasiQ0JRZ9 baseline and differential
GenevisibleiQ0JRZ9 HS

Organism-specific databases

HPAiHPA037685
HPA037686
HPA077850

Interactioni

Subunit structurei

Homodimer; disulfide-linked. May form homotetramer. Interacts with AP2A1. Interacts with EPS15, EPS15R, ITSN1 and ITSN2; recruit those scaffolding proteins which in turn may interact with the adaptor protein complex AP-2 at the plasma membrane. Interacts with DAB2 (via DPF motifs); mediates LDL receptor/LDLR endocytosis.5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi125437, 29 interactors
DIPiDIP-29488N
IntActiQ0JRZ9, 31 interactors
STRINGi9606.ENSP00000393776

Structurei

Secondary structure

1810
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ0JRZ9
SMRiQ0JRZ9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ0JRZ9

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 250F-BARPROSITE-ProRule annotationAdd BLAST248
Domaini542 – 809MHDPROSITE-ProRule annotationAdd BLAST268

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni3 – 274Mediates dimerization and binding to membranes enriched in Pi(4,5)-P2 and induces their tubulationAdd BLAST272
Regioni521 – 810Mediates interaction with DAB2, EPS15, EPS15R and ITSN1Add BLAST290

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili87 – 156Sequence analysisAdd BLAST70

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi434 – 519Ser-richAdd BLAST86

Sequence similaritiesi

Belongs to the FCHO family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2398 Eukaryota
ENOG410Y7RY LUCA
GeneTreeiENSGT00930000150876
HOGENOMiHOG000231544
HOVERGENiHBG081524
InParanoidiQ0JRZ9
KOiK20042
OMAiCFRVKNT
OrthoDBiEOG091G0AB1
PhylomeDBiQ0JRZ9
TreeFamiTF328986

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR030122 FCHo2
IPR028565 MHD
IPR018808 Muniscin_C
PANTHERiPTHR23065:SF8 PTHR23065:SF8, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF10291 muHD, 1 hit
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS51072 MHD, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q0JRZ9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVMAYFVENF WGEKNSGFDV LYHNMKHGQI STKELADFVR ERATIEEAYS
60 70 80 90 100
RSMTKLAKSA SNYSQLGTFA PVWDVFKTST EKLANCHLDL VRKLQELIKE
110 120 130 140 150
VQKYGEEQVK SHKKTKEEVA GTLEAVQTIQ SITQALQKSK ENYNAKCVEQ
160 170 180 190 200
ERLKKEGATQ REIEKAAVKS KKATDTYKLY VEKYALAKAD FEQKMTETAQ
210 220 230 240 250
KFQDIEETHL IHIKEIIGSL SNAIKEIHLQ IGQVHEEFIN NMANTTVESL
260 270 280 290 300
IQKFAESKGT GKERPGLIEF EECDTASAVE GIKPRKRKTF ALPGIIKKEK
310 320 330 340 350
DAESVECPDA DSLNIPDVDE EGYSIKPETN QNDTKENHFY SSSDSDSEDE
360 370 380 390 400
EPKKYRIEIK PMHPNNSHHT MASLDELKVS IGNITLSPAI SRHSPVQMNR
410 420 430 440 450
NLSNEELTKS KPSAPPNEKG TSDLLAWDPL FGPSLDSSSS SSLTSSSSAR
460 470 480 490 500
PTTPLSVGTI VPPPRPASRP KLTSGKLSGI NEIPRPFSPP VTSNTSPPPA
510 520 530 540 550
APLARAESSS SISSSASLSA ANTPTVGVSR GPSPVSLGNQ DTLPVAVALT
560 570 580 590 600
ESVNAYFKGA DPTKCIVKIT GDMTMSFPSG IIKVFTSNPT PAVLCFRVKN
610 620 630 640 650
ISRLEQILPN AQLVFSDPSQ CDSNTKDFWM NMQAVTVYLK KLSEQNPAAS
660 670 680 690 700
YYNVDVLKYQ VSSNGIQSTP LNLATYWKCS ASTTDLRVDY KYNPEAMVAP
710 720 730 740 750
SVLSNIQVVV PVDGGVTNMQ SLPPAIWNAE QMKAFWKLSS ISEKSENGGS
760 770 780 790 800
GSLRAKFDLS EGPSKPTTLA VQFLSEGSTL SGVDFELVGT GYRLSLIKKR
810
FATGRYLADC
Length:810
Mass (Da):88,924
Last modified:December 12, 2006 - v2
Checksum:i0FCFBC5D814B5242
GO
Isoform 2 (identifier: Q0JRZ9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     306-373: ECPDADSLNI...PNNSHHTMAS → WSFTVVAQVG...PYWPGWSRTP
     374-810: Missing.

Note: No experimental confirmation available.
Show »
Length:375
Mass (Da):43,036
Checksum:i070F5C2EE86C95CF
GO
Isoform 3 (identifier: Q0JRZ9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     200-232: Missing.

Note: No experimental confirmation available.
Show »
Length:777
Mass (Da):85,146
Checksum:iD316D1D723C3698A
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7BXJ4H7BXJ4_HUMAN
F-BAR domain only protein 2
FCHO2
378Annotation score:
H0YAE2H0YAE2_HUMAN
F-BAR domain only protein 2
FCHO2
231Annotation score:

Sequence cautioni

The sequence AAH14311 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6F → L in AL831971 (PubMed:17974005).Curated1
Sequence conflicti286K → E in BAG58162 (PubMed:14702039).Curated1
Sequence conflicti439S → P in BAF84471 (PubMed:14702039).Curated1
Sequence conflicti462P → Q in AL831971 (PubMed:17974005).Curated1
Sequence conflicti617D → Y in BAG58162 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_029636371M → V. Corresponds to variant dbSNP:rs185435Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044812200 – 232Missing in isoform 3. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_021910306 – 373ECPDA…HTMAS → WSFTVVAQVGMQWRDLGLLH SPPPRFKRFSSYLSLPSSWN YGAHHHIWLIFCIFSRDRVS PYWPGWSRTP in isoform 2. 1 PublicationAdd BLAST68
Alternative sequenceiVSP_021911374 – 810Missing in isoform 2. 1 PublicationAdd BLAST437

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK291782 mRNA Translation: BAF84471.1
AK295141 mRNA Translation: BAG58162.1
AL831971 mRNA No translation available.
AC008972 Genomic DNA No translation available.
AC020893 Genomic DNA No translation available.
AC020942 Genomic DNA No translation available.
BC014311 mRNA Translation: AAH14311.1 Different initiation.
BC137070 mRNA Translation: AAI37071.1
CCDSiCCDS47230.1 [Q0JRZ9-1]
CCDS54868.1 [Q0JRZ9-3]
RefSeqiNP_001139504.1, NM_001146032.1 [Q0JRZ9-3]
NP_620137.2, NM_138782.2 [Q0JRZ9-1]
UniGeneiHs.165762

Genome annotation databases

EnsembliENST00000430046; ENSP00000393776; ENSG00000157107 [Q0JRZ9-1]
ENST00000512348; ENSP00000427296; ENSG00000157107 [Q0JRZ9-3]
GeneIDi115548
KEGGihsa:115548
UCSCiuc003kcl.3 human [Q0JRZ9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK291782 mRNA Translation: BAF84471.1
AK295141 mRNA Translation: BAG58162.1
AL831971 mRNA No translation available.
AC008972 Genomic DNA No translation available.
AC020893 Genomic DNA No translation available.
AC020942 Genomic DNA No translation available.
BC014311 mRNA Translation: AAH14311.1 Different initiation.
BC137070 mRNA Translation: AAI37071.1
CCDSiCCDS47230.1 [Q0JRZ9-1]
CCDS54868.1 [Q0JRZ9-3]
RefSeqiNP_001139504.1, NM_001146032.1 [Q0JRZ9-3]
NP_620137.2, NM_138782.2 [Q0JRZ9-1]
UniGeneiHs.165762

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V0OX-ray2.30A/B/C3-274[»]
ProteinModelPortaliQ0JRZ9
SMRiQ0JRZ9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125437, 29 interactors
DIPiDIP-29488N
IntActiQ0JRZ9, 31 interactors
STRINGi9606.ENSP00000393776

PTM databases

iPTMnetiQ0JRZ9
PhosphoSitePlusiQ0JRZ9

Polymorphism and mutation databases

BioMutaiFCHO2
DMDMi119369487

Proteomic databases

EPDiQ0JRZ9
MaxQBiQ0JRZ9
PaxDbiQ0JRZ9
PeptideAtlasiQ0JRZ9
PRIDEiQ0JRZ9
ProteomicsDBi58761
58762 [Q0JRZ9-2]

Protocols and materials databases

DNASUi115548
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000430046; ENSP00000393776; ENSG00000157107 [Q0JRZ9-1]
ENST00000512348; ENSP00000427296; ENSG00000157107 [Q0JRZ9-3]
GeneIDi115548
KEGGihsa:115548
UCSCiuc003kcl.3 human [Q0JRZ9-1]

Organism-specific databases

CTDi115548
EuPathDBiHostDB:ENSG00000157107.13
GeneCardsiFCHO2
H-InvDBiHIX0004941
HGNCiHGNC:25180 FCHO2
HPAiHPA037685
HPA037686
HPA077850
MIMi613438 gene
neXtProtiNX_Q0JRZ9
OpenTargetsiENSG00000157107
PharmGKBiPA134911830
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2398 Eukaryota
ENOG410Y7RY LUCA
GeneTreeiENSGT00930000150876
HOGENOMiHOG000231544
HOVERGENiHBG081524
InParanoidiQ0JRZ9
KOiK20042
OMAiCFRVKNT
OrthoDBiEOG091G0AB1
PhylomeDBiQ0JRZ9
TreeFamiTF328986

Enzyme and pathway databases

ReactomeiR-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

ChiTaRSiFCHO2 human
EvolutionaryTraceiQ0JRZ9
GeneWikiiFCHO2
GenomeRNAii115548
PROiPR:Q0JRZ9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000157107 Expressed in 204 organ(s), highest expression level in mouth mucosa
CleanExiHS_FCHO2
ExpressionAtlasiQ0JRZ9 baseline and differential
GenevisibleiQ0JRZ9 HS

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR030122 FCHo2
IPR028565 MHD
IPR018808 Muniscin_C
PANTHERiPTHR23065:SF8 PTHR23065:SF8, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF10291 muHD, 1 hit
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS51072 MHD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFCHO2_HUMAN
AccessioniPrimary (citable) accession number: Q0JRZ9
Secondary accession number(s): A8K6W7
, B2RNQ9, B4DHK0, E9PG79, Q0JTJ3, Q96CF5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: November 7, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
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