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Protein

Inverted formin-2

Gene

Inf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Severs actin filaments and accelerates their polymerization and depolymerization.1 Publication

Activity regulationi

Phosphate inhibits both the depolymerization and severing activities.1 Publication

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: InterPro
  • regulation of cellular component size Source: MGI
  • regulation of mitochondrial fission Source: MGI

Keywordsi

Molecular functionActin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inverted formin-2
Gene namesi
Name:Inf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1917685 Inf2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1009L → A: Strongly inhibits depolymerization; when associated with A-1010 and A-1019. 1 Publication1
Mutagenesisi1010L → A: Strongly inhibits depolymerization; when associated with A-1009 and A-1019. 1 Publication1
Mutagenesisi1019L → A: Strongly inhibits depolymerization; when associated with A-1009 and A-1010. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002598902 – 1273Inverted formin-2Add BLAST1272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei351PhosphoserineBy similarity1
Modified residuei1172PhosphoserineCombined sources1
Modified residuei1174PhosphoserineCombined sources1
Modified residuei1203PhosphothreonineCombined sources1
Modified residuei1216PhosphoserineBy similarity1
Modified residuei1218PhosphoserineBy similarity1
Modified residuei1223PhosphothreonineCombined sources1
Modified residuei1230PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ0GNC1
MaxQBiQ0GNC1
PaxDbiQ0GNC1
PeptideAtlasiQ0GNC1
PRIDEiQ0GNC1

PTM databases

iPTMnetiQ0GNC1
PhosphoSitePlusiQ0GNC1

Interactioni

Subunit structurei

Interacts with profilin and actin at the FH1 and FH2 domains respectively.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214049, 1 interactor
DIPiDIP-61548N
IntActiQ0GNC1, 3 interactors
STRINGi10090.ENSMUSP00000098591

Structurei

3D structure databases

ProteinModelPortaliQ0GNC1
SMRiQ0GNC1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 330GBD/FH3PROSITE-ProRule annotationAdd BLAST329
Domaini421 – 564FH1Add BLAST144
Domaini589 – 979FH2PROSITE-ProRule annotationAdd BLAST391
Domaini1007 – 1022WH2PROSITE-ProRule annotationAdd BLAST16

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili907 – 984Sequence analysisAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi421 – 563Pro-richAdd BLAST143

Domaini

The WH2 domain acts as the DAD (diaphanous autoregulatory) domain and binds to actin monomers.1 Publication
Regulated by autoinhibition due to intramolecular GBD-DAD binding.1 Publication
The severing activity is dependent on covalent attachment of the FH2 domain to the C-terminus.1 Publication

Sequence similaritiesi

Belongs to the formin homology family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1922 Eukaryota
ENOG410XQR0 LUCA
HOGENOMiHOG000113072
HOVERGENiHBG081794
InParanoidiQ0GNC1
PhylomeDBiQ0GNC1

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
IPR027649 Inf2
IPR003124 WH2_dom
PANTHERiPTHR23213:SF5 PTHR23213:SF5, 2 hits
PfamiView protein in Pfam
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 1 hit
PF02181 FH2, 1 hit
PF02205 WH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit
PS51082 WH2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q0GNC1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSVKEGAQRK WAALKEKLGP QDSDPTEANL ESAEPELCIR LLQMPSVVNY
60 70 80 90 100
SGLRKRLESS DGGWMVQFLE QSGLDLLLEA LARLSGRGVA RISDALLQLT
110 120 130 140 150
CISCVRAVMN SQQGIEYILS NQGYVRQLSQ ALDTSNVMVK KQVFELLAAL
160 170 180 190 200
CIYSPEGHAL TLDALDHYKM VCSQQYRFSV IMSELSDSDN VPYVVTLLSV
210 220 230 240 250
INAIILGPED LRSRAQLRSE FIGLQLLDIL TRLRDLEDAD LLIQLEAFEE
260 270 280 290 300
AKAEDEEELQ RISDGINMNS HQEVFASLFH KVSCSPASAQ LLSVLQGLMH
310 320 330 340 350
LEPAGRSGQL LWEALENLVN RAVLLASDAQ ACTLEEVVER LLSIKGRPRP
360 370 380 390 400
SPLDKAHKSV QTNSVQNQGS SSQNTTTPTT KVEGQQPVVA SPCQHVGSIQ
410 420 430 440 450
SSSVDIAPQP VALEQCITAL PLPTPPLSSS TPVLPPTPPP LPGPGATSPL
460 470 480 490 500
PPPPPPLPPP LPGSGTTSPP PPPPPPPPLP PPLPGSGTIS PPPPPPPPPL
510 520 530 540 550
PGTGAVSPPP PPPLPSLPDS HKTQPPPPPP PPLPGMCPVP PPPPLPRAGQ
560 570 580 590 600
IPPPPPLPGF SVPSMMGGVE EIIVAQVDHS LGSAWVPSHR RVNPPTLRMK
610 620 630 640 650
KLNWQKLPSN VARERNSMWA TLGSPCTAAV EPDFSSIEQL FSFPTAKPKE
660 670 680 690 700
PSAAPARKEP KEVTFLDSKK SLNLNIFLKQ FKCSNEEVTS MIQAGDTSKF
710 720 730 740 750
DVEVLKQLLK LLPEKHEIEN LRAFTEERAK LSNADQFYVL LLDIPCYPLR
760 770 780 790 800
VECMMLCEGT AIVLDMVRPK AQLVLTACES LLTSQRLPVF CQLILKIGNF
810 820 830 840 850
LNYGSHTGDA DGFKISTLLK LTETKSQQSR VTLLHHVLEE VEKSHPDLLQ
860 870 880 890 900
LSRDLEPPSQ AAGINVEIIH SEASANLKKL LEAERKVSAS IPEVQKQYAE
910 920 930 940 950
RLQASIEASQ ELDKVFDAIE QKKLELADYL CEDPQQLSLE DTFSTMKTFR
960 970 980 990 1000
DLFTRALKEN KDRKEQMAKA ERRKQQLAEE EARRPRDEDG KPIRKGPGKQ
1010 1020 1030 1040 1050
EEVCVIDALL ADIRKGFQLR KTARGRGDTE ASGRVAPTDP PKATEPATAS
1060 1070 1080 1090 1100
NPTQGTNHPA SEPLDTTAAD EPQGWDLVDA VTPSPQPSKE EDGPPALERR
1110 1120 1130 1140 1150
SSWYVDAIDF LDPEDTPDAQ PSEGVWPVTL GDGQALNPLE FSSNKPPGVK
1160 1170 1180 1190 1200
SSHQDATDPE ALWGVHQTEA DSTSEGPEDE AQRGQSTHLP RTGPGEDEDG
1210 1220 1230 1240 1250
EDTAPESALD TSLDRSFSED AVTDSSGSGT LPRVQGRVSK GTSKRRKKRP
1260 1270
SRNQEEFVPD SDDIKAKRLC VIQ
Length:1,273
Mass (Da):138,560
Last modified:October 3, 2006 - v1
Checksum:iC4438AD8A7041476
GO
Isoform 2 (identifier: Q0GNC1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1256-1273: EFVPDSDDIKAKRLCVIQ → GLRSRPKAK

Note: No experimental confirmation available.
Show »
Length:1,264
Mass (Da):137,496
Checksum:iCC8EEF55F65801CB
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QLA5E9QLA5_MOUSE
Inverted formin-2
Inf2
1,271Annotation score:
A0A1Y7VM80A0A1Y7VM80_MOUSE
Inverted formin-2
Inf2
720Annotation score:
A0A1Y7VLQ6A0A1Y7VLQ6_MOUSE
Inverted formin-2
Inf2
24Annotation score:

Sequence cautioni

The sequence AAH60610 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH99931 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1038T → A in AAH60610 (PubMed:15489334).Curated1
Sequence conflicti1038T → A in AAI15423 (PubMed:15489334).Curated1
Sequence conflicti1167Q → R in AAH60610 (PubMed:15489334).Curated1
Sequence conflicti1167Q → R in AAI15423 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0215601256 – 1273EFVPD…LCVIQ → GLRSRPKAK in isoform 2. 1 PublicationAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ834374 mRNA Translation: ABI20145.1
BC060610 mRNA Translation: AAH60610.1 Different initiation.
BC099931 mRNA Translation: AAH99931.1 Different initiation.
BC115422 mRNA Translation: AAI15423.2
BC115423 mRNA Translation: AAI15424.2
RefSeqiNP_940803.2, NM_198411.2
UniGeneiMm.250193

Genome annotation databases

GeneIDi70435
KEGGimmu:70435

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ834374 mRNA Translation: ABI20145.1
BC060610 mRNA Translation: AAH60610.1 Different initiation.
BC099931 mRNA Translation: AAH99931.1 Different initiation.
BC115422 mRNA Translation: AAI15423.2
BC115423 mRNA Translation: AAI15424.2
RefSeqiNP_940803.2, NM_198411.2
UniGeneiMm.250193

3D structure databases

ProteinModelPortaliQ0GNC1
SMRiQ0GNC1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214049, 1 interactor
DIPiDIP-61548N
IntActiQ0GNC1, 3 interactors
STRINGi10090.ENSMUSP00000098591

PTM databases

iPTMnetiQ0GNC1
PhosphoSitePlusiQ0GNC1

Proteomic databases

EPDiQ0GNC1
MaxQBiQ0GNC1
PaxDbiQ0GNC1
PeptideAtlasiQ0GNC1
PRIDEiQ0GNC1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi70435
KEGGimmu:70435

Organism-specific databases

CTDi64423
MGIiMGI:1917685 Inf2

Phylogenomic databases

eggNOGiKOG1922 Eukaryota
ENOG410XQR0 LUCA
HOGENOMiHOG000113072
HOVERGENiHBG081794
InParanoidiQ0GNC1
PhylomeDBiQ0GNC1

Miscellaneous databases

PROiPR:Q0GNC1
SOURCEiSearch...

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
IPR027649 Inf2
IPR003124 WH2_dom
PANTHERiPTHR23213:SF5 PTHR23213:SF5, 2 hits
PfamiView protein in Pfam
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 1 hit
PF02181 FH2, 1 hit
PF02205 WH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit
PS51082 WH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiINF2_MOUSE
AccessioniPrimary (citable) accession number: Q0GNC1
Secondary accession number(s): Q14C56, Q499F7, Q6P9T3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: October 3, 2006
Last modified: September 12, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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