UniProtKB - Q0E940 (EIF3B_DROME)
Protein
Eukaryotic translation initiation factor 3 subunit B
Gene
eIF3b
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.UniRule annotation
GO - Molecular functioni
- mRNA binding Source: FlyBase
- translation initiation factor activity Source: UniProtKB
- translation initiation factor binding Source: InterPro
GO - Biological processi
- formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
- ovarian follicle cell development Source: FlyBase
- regulation of translational initiation Source: UniProtKB
- translational initiation Source: FlyBase
Keywordsi
Molecular function | Initiation factor, RNA-binding |
Biological process | Protein biosynthesis |
Enzyme and pathway databases
Reactomei | R-DME-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-DME-72649, Translation initiation complex formation R-DME-72689, Formation of a pool of free 40S subunits R-DME-72695, Formation of the ternary complex, and subsequently, the 43S complex R-DME-72702, Ribosomal scanning and start codon recognition R-DME-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 3 subunit BUniRule annotationShort name: eIF3bUniRule annotation Alternative name(s): Eukaryotic translation initiation factor 3 subunit 9UniRule annotation |
Gene namesi | ORF Names:CG4878Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0034237, eIF3b |
Subcellular locationi
Other locations
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: FlyBase
Other locations
- eukaryotic 43S preinitiation complex Source: UniProtKB-UniRule
- eukaryotic 48S preinitiation complex Source: UniProtKB-UniRule
- eukaryotic translation initiation factor 3 complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000363797 | 1 – 690 | Eukaryotic translation initiation factor 3 subunit BAdd BLAST | 690 |
Proteomic databases
PaxDbi | Q0E940 |
PRIDEi | Q0E940 |
Expressioni
Gene expression databases
Bgeei | FBgn0034237, Expressed in wing disc (Drosophila) and 50 other tissues |
ExpressionAtlasi | Q0E940, baseline and differential |
Genevisiblei | Q0E940, DM |
Interactioni
Subunit structurei
GO - Molecular functioni
- translation initiation factor binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 62670, 29 interactors |
IntActi | Q0E940, 9 interactors |
STRINGi | 7227.FBpp0086098 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 57 – 141 | RRMUniRule annotationAdd BLAST | 85 | |
Repeati | 207 – 246 | WD 1Add BLAST | 40 | |
Repeati | 293 – 331 | WD 2Add BLAST | 39 | |
Repeati | 334 – 369 | WD 3Add BLAST | 36 | |
Repeati | 442 – 484 | WD 4Add BLAST | 43 | |
Repeati | 530 – 575 | WD 5Add BLAST | 46 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 595 – 645 | UniRule annotationAdd BLAST | 51 |
Sequence similaritiesi
Belongs to the eIF-3 subunit B family.UniRule annotation
Keywords - Domaini
Coiled coil, Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG2314, Eukaryota |
GeneTreei | ENSGT00550000074913 |
HOGENOMi | CLU_011152_1_0_1 |
InParanoidi | Q0E940 |
OMAi | LIDFSPN |
OrthoDBi | 1194797at2759 |
PhylomeDBi | Q0E940 |
Family and domain databases
CDDi | cd12278, RRM_eIF3B, 1 hit |
Gene3Di | 2.130.10.10, 1 hit 3.30.70.330, 1 hit |
HAMAPi | MF_03001, eIF3b, 1 hit |
InterProi | View protein in InterPro IPR011400, EIF3B IPR034363, eIF3B_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR013979, TIF_beta_prop-like IPR015943, WD40/YVTN_repeat-like_dom_sf |
PANTHERi | PTHR14068, PTHR14068, 1 hit |
Pfami | View protein in Pfam PF08662, eIF2A, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF036424, eIF3b, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q0E940-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKKKSEEHS GTDANDSDYQ EEPNFDDPPG FVDNISDEDL LGDMLAQRPS
60 70 80 90 100
EADGVESVVV VDNIPKVEPV RLEKLKLVIN KLFSNYGEIV NVVYPVDEEG
110 120 130 140 150
KTKGYAFMEY KQARQAEEAV KKLNNHRLDK NHTFAVNLFT DFQKYENIPE
160 170 180 190 200
KWEPPTVQTF KVQSDLYNFI NDPDTYDQYC VAAETAPNCV QVGFWQNVLP
210 220 230 240 250
EPFELETRER FTDTFVKWSP LGTYVVTFHK PGVAIWGGSS FQKIQKFPHP
260 270 280 290 300
GTQFVEFSPC ENYLVTYGPT PTGQKIIIWD IRTGAEKRSF VADGMSVLSM
310 320 330 340 350
FRWSHDDKFV ARMGENSIHI YETPSFYLLD LKSIKIPGIR GFSWSPTDNV
360 370 380 390 400
IAYWVEEQNQ IPARVTLMEI PKKREIRNKN LFHVADCKLH WQKSGDYLCV
410 420 430 440 450
KVDRYSKLKK DKKDLDVKFL GMFYNFEIFH MREKEIPVDS VEIRELILAF
460 470 480 490 500
AWEPVGNKFS IIHGETNSSN VSFYEVNKGV KPSLVKRLEK KSCTHLFWSP
510 520 530 540 550
RGQFIVMANL TMGTFEFVDS TNDYIITASP DHFRASEVEW DPTGRYVVTG
560 570 580 590 600
VSSWKVKEDT GFNMYTFQGR IIKRTILKNF VQFLWRPRPP TLLSEEKQKE
610 620 630 640 650
IKKNLKKYYA AFEQKDRLRL TRASKELLEK RSQLRETFME YRNKRIAEWA
660 670 680 690
EQKSRRIMLR GHVDTDNLET DEVDEEIVEF LVKEEVTLLE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE2QCG7 | E2QCG7_DROME | Eukaryotic translation initiation f... | eIF3b cg4878, Dmel\CG4878, eIF3 S9, eIF3-S9, eIF3-s9 | 690 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 12 | T → A in AAM52578 (PubMed:12537569).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF57842.1 AY122066 mRNA Translation: AAM52578.1 BT072816 mRNA Translation: ACN62419.1 |
RefSeqi | NP_611228.1, NM_137384.3 NP_725691.1, NM_166239.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0086941; FBpp0086097; FBgn0034237 FBtr0086942; FBpp0086098; FBgn0034237 |
GeneIDi | 36981 |
KEGGi | dme:Dmel_CG4878 |
UCSCi | CG4878-RA, d. melanogaster CG4878-RB, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF57842.1 AY122066 mRNA Translation: AAM52578.1 BT072816 mRNA Translation: ACN62419.1 |
RefSeqi | NP_611228.1, NM_137384.3 NP_725691.1, NM_166239.4 |
3D structure databases
SMRi | Q0E940 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 62670, 29 interactors |
IntActi | Q0E940, 9 interactors |
STRINGi | 7227.FBpp0086098 |
Proteomic databases
PaxDbi | Q0E940 |
PRIDEi | Q0E940 |
Genome annotation databases
EnsemblMetazoai | FBtr0086941; FBpp0086097; FBgn0034237 FBtr0086942; FBpp0086098; FBgn0034237 |
GeneIDi | 36981 |
KEGGi | dme:Dmel_CG4878 |
UCSCi | CG4878-RA, d. melanogaster CG4878-RB, d. melanogaster |
Organism-specific databases
CTDi | 8662 |
FlyBasei | FBgn0034237, eIF3b |
Phylogenomic databases
eggNOGi | KOG2314, Eukaryota |
GeneTreei | ENSGT00550000074913 |
HOGENOMi | CLU_011152_1_0_1 |
InParanoidi | Q0E940 |
OMAi | LIDFSPN |
OrthoDBi | 1194797at2759 |
PhylomeDBi | Q0E940 |
Enzyme and pathway databases
Reactomei | R-DME-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-DME-72649, Translation initiation complex formation R-DME-72689, Formation of a pool of free 40S subunits R-DME-72695, Formation of the ternary complex, and subsequently, the 43S complex R-DME-72702, Ribosomal scanning and start codon recognition R-DME-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
Miscellaneous databases
BioGRID-ORCSi | 36981, 1 hit in 1 CRISPR screen |
GenomeRNAii | 36981 |
PROi | PR:Q0E940 |
Gene expression databases
Bgeei | FBgn0034237, Expressed in wing disc (Drosophila) and 50 other tissues |
ExpressionAtlasi | Q0E940, baseline and differential |
Genevisiblei | Q0E940, DM |
Family and domain databases
CDDi | cd12278, RRM_eIF3B, 1 hit |
Gene3Di | 2.130.10.10, 1 hit 3.30.70.330, 1 hit |
HAMAPi | MF_03001, eIF3b, 1 hit |
InterProi | View protein in InterPro IPR011400, EIF3B IPR034363, eIF3B_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR013979, TIF_beta_prop-like IPR015943, WD40/YVTN_repeat-like_dom_sf |
PANTHERi | PTHR14068, PTHR14068, 1 hit |
Pfami | View protein in Pfam PF08662, eIF2A, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF036424, eIF3b, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EIF3B_DROME | |
Accessioni | Q0E940Primary (citable) accession number: Q0E940 Secondary accession number(s): C0PUW0, Q8MR84 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 10, 2009 |
Last sequence update: | October 17, 2006 | |
Last modified: | February 10, 2021 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families - Translation initiation factors
List of translation initiation factor entries