UniProtKB - Q0DDE3 (SSY23_ORYSJ)
Protein
Soluble starch synthase 2-3, chloroplastic/amyloplastic
Gene
SSII-3
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Plays an important role during endosperm starch synthesis. Determines the type of amylopectin structure of starch grain. Synthesizes long B1 amylopectin chains by elongating short A and B1 chains, independently of the other soluble starch synthases. Barely active in japonica subspecies.2 Publications
Miscellaneous
Increased activity due to variations in subspecies indica is associated with the synthesis of the L-type amylopectin, which contains much lower proportion of short amylopectin chains than the S-type amylopectin found in most japonica varieties. The difference in amylopectin chain composition explains why starch from japonica subspecies has a lower gelatinisation temperature than starch from indica subspecies.
Catalytic activityi
- EC:2.4.1.21
: starch biosynthesis Pathwayi
This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 333 | ADP-glucoseBy similarity | 1 |
GO - Molecular functioni
- alpha-1,4-glucan synthase activity Source: UniProtKB-EC
- glycogen (starch) synthase activity Source: InterPro
- starch synthase activity Source: UniProtKB-EC
GO - Biological processi
- starch biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Starch biosynthesis |
Enzyme and pathway databases
PlantReactomei | R-OSA-9626305, Regulatory network of nutrient accumulation |
UniPathwayi | UPA00152 |
Protein family/group databases
CAZyi | GT5, Glycosyltransferase Family 5 |
Names & Taxonomyi
Protein namesi | Recommended name: Soluble starch synthase 2-3, chloroplastic/amyloplastic (EC:2.4.1.21)Alternative name(s): Soluble starch synthase II-3 Starch synthase IIa |
Gene namesi | Name:SSII-3 Synonyms:ALK Ordered Locus Names:Os06g0229800, LOC_Os06g12450 ORF Names:P0525F01.23 |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Subcellular locationi
Chloroplast
Other locations
Note: Amyloplast or chloroplast, granule-bound and soluble.
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Other locations
- amyloplast Source: UniProtKB-SubCell
Keywords - Cellular componenti
Amyloplast, Chloroplast, PlastidPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 88 | D → E: Increased activity; when associated with V-737. 1 Publication | 1 | |
Mutagenesisi | 604 | S → G: Increased activity; when associated with V-737. Slightly increased activity; when associated with V-737 and F-781. 1 Publication | 1 | |
Mutagenesisi | 737 | M → V: Increased activity. Increased activity; when associated with E-88 and/or G-604. Slightly increased activity; when associated with G-604 and F-781. 1 Publication | 1 | |
Mutagenesisi | 781 | L → F: Slightly increased activity; when associated with G-604 and V-737. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 16 | ChloroplastSequence analysisAdd BLAST | 16 | |
ChainiPRO_0000011141 | 17 – 810 | Soluble starch synthase 2-3, chloroplastic/amyloplasticAdd BLAST | 794 |
Proteomic databases
PaxDbi | Q0DDE3 |
PRIDEi | Q0DDE3 |
Expressioni
Tissue specificityi
Expressed most exclusively in endosperm.2 Publications
Developmental stagei
Expressed in developing caryopsis at 5 to 15 days after flowering. Expressed exclusively in the endosperm at 5 to 10 days after flowering.1 Publication
Gene expression databases
ExpressionAtlasi | Q0DDE3, baseline and differential |
Genevisiblei | Q0DDE3, OS |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 53 – 60 | Poly-Pro | 8 | |
Compositional biasi | 178 – 235 | Pro-richAdd BLAST | 58 | |
Compositional biasi | 278 – 309 | Asp-richAdd BLAST | 32 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | ENOG502QT35, Eukaryota |
HOGENOMi | CLU_009583_31_0_1 |
InParanoidi | Q0DDE3 |
OMAi | QDVGIRK |
OrthoDBi | 732319at2759 |
Family and domain databases
HAMAPi | MF_00484, Glycogen_synth, 1 hit |
InterProi | View protein in InterPro IPR001296, Glyco_trans_1 IPR011835, GS/SS IPR013534, Starch_synth_cat_dom |
Pfami | View protein in Pfam PF08323, Glyco_transf_5, 1 hit PF00534, Glycos_transf_1, 1 hit |
TIGRFAMsi | TIGR02095, glgA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q0DDE3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSAVVASST TFLVALASSA SRGGPRRGRV VGVAAPPALL YDGRAGRLAL
60 70 80 90 100
RAPPPPRPRP RRRDAGVVRR ADDGENEAAV ERAGEDDDEE EEFSSGAWQP
110 120 130 140 150
PRSRRGGVGK VLKRRGTVPP VGRYGSGGDA ARVRGAAAPA PAPTQDAASS
160 170 180 190 200
KNGALLSGRD DDTPASRNGS VVTGADKPAA ATPPVTITKL PAPDSPVILP
210 220 230 240 250
SVDKPQPEFV IPDATAPAPP PPGSNPRSSA PLPKPDNSEF AEDKSAKVVE
260 270 280 290 300
SAPKPKATRS SPIPAVEEET WDFKKYFDLN EPDAAEDGDD DDDWADSDAS
310 320 330 340 350
DSEIDQDDDS GPLAGENVMN VIVVAAECSP WCKTGGLGDV AGALPKALAR
360 370 380 390 400
RGHRVMVVVP RYGDYAEAQD VGIRKYYKAA GQDLEVKYFH AFIDGVDFVF
410 420 430 440 450
IDAPLFRHRQ DDIYGGNRQE IMKRMILFCK AAVEVPWHVP CGGVPYGDGN
460 470 480 490 500
LVFLANDWHT ALLPVYLKAY YRDNGMMQYT RSVLVIHNIA YQGRGPVDEF
510 520 530 540 550
PYMELPEHYL DHFKLYDPVG GEHANIFGAG LKMADRVVTV SPGYLWELKT
560 570 580 590 600
TEGGWGLHDI IRENDWKMNG IVNGIDYREW NPEVDVHLQS DGYANYTVAS
610 620 630 640 650
LDSSKPRCKA ALQRELGLEV RDDVPLIGFI GRLDGQKGVD IIGDAMPWIA
660 670 680 690 700
GQDVQLVLLG SGRRDLEVML QRFEAQHNSK VRGWVGFSVK MAHRITAGAD
710 720 730 740 750
VLVMPSRFEP CGLNQLYAMA YGTVPVVHAV GGLRDTMSAF DPFEDTGLGW
760 770 780 790 800
TFDRAEPHKL IEALGHCLET YRKYKESWRG LQVRGMSQDL SWDHAAELYE
810
EVLVKAKYQW
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 737 | M → V in strain: cv. Kinmaze. 1 Publication | 1 | |
Natural varianti | 781 | L → F in strain: cv. Kinmaze. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF419099 mRNA Translation: AAL16661.1 AB115915 mRNA Translation: BAD90591.1 AB115916 mRNA Translation: BAD90592.1 AP003509 Genomic DNA Translation: BAD37272.1 AP008212 Genomic DNA Translation: BAF19130.1 AP014962 Genomic DNA Translation: BAS96901.1 |
RefSeqi | XP_015644246.1, XM_015788760.1 |
Genome annotation databases
EnsemblPlantsi | Os06t0229800-01; Os06t0229800-01; Os06g0229800 |
GeneIDi | 4340567 |
Gramenei | Os06t0229800-01; Os06t0229800-01; Os06g0229800 |
KEGGi | osa:4340567 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF419099 mRNA Translation: AAL16661.1 AB115915 mRNA Translation: BAD90591.1 AB115916 mRNA Translation: BAD90592.1 AP003509 Genomic DNA Translation: BAD37272.1 AP008212 Genomic DNA Translation: BAF19130.1 AP014962 Genomic DNA Translation: BAS96901.1 |
RefSeqi | XP_015644246.1, XM_015788760.1 |
3D structure databases
SMRi | Q0DDE3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4530.OS06T0229800-01 |
Protein family/group databases
CAZyi | GT5, Glycosyltransferase Family 5 |
Proteomic databases
PaxDbi | Q0DDE3 |
PRIDEi | Q0DDE3 |
Genome annotation databases
EnsemblPlantsi | Os06t0229800-01; Os06t0229800-01; Os06g0229800 |
GeneIDi | 4340567 |
Gramenei | Os06t0229800-01; Os06t0229800-01; Os06g0229800 |
KEGGi | osa:4340567 |
Phylogenomic databases
eggNOGi | ENOG502QT35, Eukaryota |
HOGENOMi | CLU_009583_31_0_1 |
InParanoidi | Q0DDE3 |
OMAi | QDVGIRK |
OrthoDBi | 732319at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00152 |
PlantReactomei | R-OSA-9626305, Regulatory network of nutrient accumulation |
Gene expression databases
ExpressionAtlasi | Q0DDE3, baseline and differential |
Genevisiblei | Q0DDE3, OS |
Family and domain databases
HAMAPi | MF_00484, Glycogen_synth, 1 hit |
InterProi | View protein in InterPro IPR001296, Glyco_trans_1 IPR011835, GS/SS IPR013534, Starch_synth_cat_dom |
Pfami | View protein in Pfam PF08323, Glyco_transf_5, 1 hit PF00534, Glycos_transf_1, 1 hit |
TIGRFAMsi | TIGR02095, glgA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SSY23_ORYSJ | |
Accessioni | Q0DDE3Primary (citable) accession number: Q0DDE3 Secondary accession number(s): Q5DWW9 Q944W5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 24, 2007 |
Last sequence update: | October 17, 2006 | |
Last modified: | December 2, 2020 | |
This is version 88 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families