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Protein

Cytosolic carboxypeptidase 6

Gene

Agbl4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Metallocarboxypeptidase that mediates deglutamylation of target proteins (PubMed:17244818, PubMed:21074048, PubMed:25103237, PubMed:26829768). Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins (PubMed:17244818). Also removes polyglutamates from the carboxy-terminus of target proteins such as MYLK (PubMed:21074048). Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS (PubMed:26829768). Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5 (PubMed:25103237).4 Publications

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi230Zinc1 Publication1
Metal bindingi233Zinc1 Publication1
Active sitei280NucleophileBy similarity1
Metal bindingi328ZincBy similarity1

GO - Molecular functioni

  • metallocarboxypeptidase activity Source: UniProtKB
  • tubulin binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • C-terminal protein deglutamylation Source: UniProtKB
  • defense response to virus Source: UniProtKB
  • protein deglutamylation Source: UniProtKB
  • protein side chain deglutamylation Source: UniProtKB

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic carboxypeptidase 6 (EC:3.4.17.-)
Alternative name(s):
ATP/GTP-binding protein-like 4
Gene namesi
Name:Agbl4
Synonyms:Ccp6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1918244 Agbl4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus

Pathology & Biotechi

Disruption phenotypei

Mice are more vulnerable to DNA virus infection due to impaired immune response.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi230H → S: Abolishes deglutamylase activity; when associated with Q-233. 2 Publications1
Mutagenesisi233E → Q: Abolishes deglutamylase activity; when associated with S-230. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002848361 – 540Cytosolic carboxypeptidase 6Add BLAST540

Proteomic databases

PaxDbiQ09LZ8
PRIDEiQ09LZ8

PTM databases

iPTMnetiQ09LZ8
PhosphoSitePlusiQ09LZ8

Expressioni

Tissue specificityi

Widely expressed. Expressed abundantly in testis, pituitary and brain and to a lower extent in eye, stomach, adrenal and kidney. In brain, expressed at low level in cerebellum as compared to cortex.3 Publications

Gene expression databases

BgeeiENSMUSG00000061298 Expressed in 83 organ(s), highest expression level in bone marrow
ExpressionAtlasiQ09LZ8 baseline and differential
GenevisibleiQ09LZ8 MM

Interactioni

Subunit structurei

Interacts with MYLK.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095533

Structurei

3D structure databases

ProteinModelPortaliQ09LZ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Phylogenomic databases

eggNOGiKOG3641 Eukaryota
COG2866 LUCA
GeneTreeiENSGT00550000074405
HOGENOMiHOG000231164
HOVERGENiHBG107588
InParanoidiQ09LZ8
OMAiKWQRIPR
OrthoDBiEOG091G0FXK
PhylomeDBiQ09LZ8
TreeFamiTF333009

Family and domain databases

InterProiView protein in InterPro
IPR000834 Peptidase_M14
PfamiView protein in Pfam
PF00246 Peptidase_M14, 1 hit
SMARTiView protein in SMART
SM00631 Zn_pept, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q09LZ8-1) [UniParc]FASTAAdd to basket
Also known as: 2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAERSQTAPE AGNDTGNEDA IGGNVNKYIV LPNGYSGQPK KGHLTFDACF
60 70 80 90 100
ESGNLGRVEQ VSDFEYDLFI RPDTCNPRFR VWFNFTVENV KELQRVIFNI
110 120 130 140 150
VNFSKTKSLY RDGMAPMVKS TSRPKWQRLP PKNVYYYRCP DHRKNYVMSF
160 170 180 190 200
AFCFDREDDI YQFAYCYPYT YTRFQHYLDS LQKKNMDYFF REQLGQSVQQ
210 220 230 240 250
RQLDLLTITS PENLREGSEK KVIFITGRVH PGETPSSFVC QGIIDFLVSQ
260 270 280 290 300
HPIARVLREH LVFKIAPMLN PDGVYLGNYR CSLMGFDLNR HWLDPSPWAH
310 320 330 340 350
PTLHGVKQLI IKMYNDPKTS LEFYIDIHAH STMMNGFMYG NIFEDEERFQ
360 370 380 390 400
RQSIFPKLLC QNAEDFSYTS TSFNRDAVKA GTGRRFLGGL LDHSSYCYTL
410 420 430 440 450
EVSFYSYIIG GTTAAVPYTE EAYMKLGRNV ARTFLDYYRL NSLVEKIAVP
460 470 480 490 500
MPRLRSKEER RLGWEHPSCL RAEQPLEVLG IPMCSGKALN EHLGNDIQWH
510 520 530 540
LDCGSSTLPL GLISCSPSSS ASWNDMAMSN SILLPDHSFH
Length:540
Mass (Da):62,210
Last modified:October 17, 2006 - v1
Checksum:iD9549A180D906E24
GO
Isoform 2 (identifier: Q09LZ8-2) [UniParc]FASTAAdd to basket
Also known as: 1

The sequence of this isoform differs from the canonical sequence as follows:
     456-463: SKEERRLG → FRLQRTHL
     464-540: Missing.

Note: No experimental confirmation available.
Show »
Length:463
Mass (Da):53,952
Checksum:iC97B18D1E6D12A9B
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A9S7A2A9S7_MOUSE
Cytosolic carboxypeptidase 6
Agbl4
346Annotation score:
E4W7Y4E4W7Y4_MOUSE
CCP6
Agbl4
494Annotation score:
F6VQN0F6VQN0_MOUSE
Cytosolic carboxypeptidase 6
Agbl4
277Annotation score:
A2A9S6A2A9S6_MOUSE
Cytosolic carboxypeptidase 6
Agbl4
164Annotation score:
A2A9S5A2A9S5_MOUSE
Cytosolic carboxypeptidase 6
Agbl4
63Annotation score:

Sequence cautioni

The sequence CAM13964 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM16884 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM16953 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM17710 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM17858 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM19669 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM21591 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM21666 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024671456 – 463SKEERRLG → FRLQRTHL in isoform 2. 1 Publication8
Alternative sequenceiVSP_024672464 – 540Missing in isoform 2. 1 PublicationAdd BLAST77

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ867039 mRNA Translation: ABI51958.1
DQ867040 mRNA Translation: ABI51959.1
AL627347
, AL627183, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAM13964.1 Sequence problems.
AL669965
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL671895 Genomic DNA Translation: CAM16884.1 Sequence problems.
AL662829
, AL627183, AL627347, AL627425, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAM16953.1 Sequence problems.
AL669940
, AL627183, AL627347, AL627425, AL662829, AL669959, AL669965, AL671895 Genomic DNA Translation: CAM17710.1 Sequence problems.
AL669959
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669965, AL671895 Genomic DNA Translation: CAM17858.1 Sequence problems.
AL627425
, AL627183, AL627347, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAM19669.1 Sequence problems.
AL671895
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965 Genomic DNA Translation: CAM21591.1 Sequence problems.
AL627183
, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAM21666.1 Sequence problems.
AL627425
, AL627183, AL627347, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO77735.1
AL669940
, AL627183, AL627347, AL627425, AL662829, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO77918.1
AL669959
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669965, AL671895 Genomic DNA Translation: CAO77920.1
AL671895
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965 Genomic DNA Translation: CAO78078.1
AL662829
, AL627183, AL627347, AL627425, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO78121.1
AL627183
, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAP19222.1
AL627347
, AL627183, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAQ11261.1
AL669965
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL671895 Genomic DNA Translation: CAQ13051.1
AL627425
, AL627183, AL627347, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO77734.1
AL669940
, AL627183, AL627347, AL627425, AL662829, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO77917.1
AL669959
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669965, AL671895 Genomic DNA Translation: CAO77919.1
AL671895
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965 Genomic DNA Translation: CAO78077.1
AL662829
, AL627183, AL627347, AL627425, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO78120.1
AL627183
, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAP19221.1
AL627347
, AL627183, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAQ11260.1
AL669965
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL671895 Genomic DNA Translation: CAQ13050.1
CCDSiCCDS51263.1 [Q09LZ8-2]
CCDS51264.1 [Q09LZ8-1]
RefSeqiNP_001041654.2, NM_001048189.4 [Q09LZ8-2]
NP_084507.1, NM_030231.3 [Q09LZ8-1]
UniGeneiMm.435620

Genome annotation databases

EnsembliENSMUST00000080744; ENSMUSP00000079568; ENSMUSG00000061298 [Q09LZ8-2]
ENSMUST00000097920; ENSMUSP00000095533; ENSMUSG00000061298 [Q09LZ8-1]
GeneIDi78933
KEGGimmu:78933
UCSCiuc008udc.2 mouse [Q09LZ8-1]
uc008udd.2 mouse [Q09LZ8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ867039 mRNA Translation: ABI51958.1
DQ867040 mRNA Translation: ABI51959.1
AL627347
, AL627183, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAM13964.1 Sequence problems.
AL669965
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL671895 Genomic DNA Translation: CAM16884.1 Sequence problems.
AL662829
, AL627183, AL627347, AL627425, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAM16953.1 Sequence problems.
AL669940
, AL627183, AL627347, AL627425, AL662829, AL669959, AL669965, AL671895 Genomic DNA Translation: CAM17710.1 Sequence problems.
AL669959
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669965, AL671895 Genomic DNA Translation: CAM17858.1 Sequence problems.
AL627425
, AL627183, AL627347, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAM19669.1 Sequence problems.
AL671895
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965 Genomic DNA Translation: CAM21591.1 Sequence problems.
AL627183
, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAM21666.1 Sequence problems.
AL627425
, AL627183, AL627347, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO77735.1
AL669940
, AL627183, AL627347, AL627425, AL662829, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO77918.1
AL669959
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669965, AL671895 Genomic DNA Translation: CAO77920.1
AL671895
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965 Genomic DNA Translation: CAO78078.1
AL662829
, AL627183, AL627347, AL627425, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO78121.1
AL627183
, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAP19222.1
AL627347
, AL627183, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAQ11261.1
AL669965
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL671895 Genomic DNA Translation: CAQ13051.1
AL627425
, AL627183, AL627347, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO77734.1
AL669940
, AL627183, AL627347, AL627425, AL662829, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO77917.1
AL669959
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669965, AL671895 Genomic DNA Translation: CAO77919.1
AL671895
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965 Genomic DNA Translation: CAO78077.1
AL662829
, AL627183, AL627347, AL627425, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAO78120.1
AL627183
, AL627347, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAP19221.1
AL627347
, AL627183, AL627425, AL662829, AL669940, AL669959, AL669965, AL671895 Genomic DNA Translation: CAQ11260.1
AL669965
, AL627183, AL627347, AL627425, AL662829, AL669940, AL669959, AL671895 Genomic DNA Translation: CAQ13050.1
CCDSiCCDS51263.1 [Q09LZ8-2]
CCDS51264.1 [Q09LZ8-1]
RefSeqiNP_001041654.2, NM_001048189.4 [Q09LZ8-2]
NP_084507.1, NM_030231.3 [Q09LZ8-1]
UniGeneiMm.435620

3D structure databases

ProteinModelPortaliQ09LZ8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095533

PTM databases

iPTMnetiQ09LZ8
PhosphoSitePlusiQ09LZ8

Proteomic databases

PaxDbiQ09LZ8
PRIDEiQ09LZ8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080744; ENSMUSP00000079568; ENSMUSG00000061298 [Q09LZ8-2]
ENSMUST00000097920; ENSMUSP00000095533; ENSMUSG00000061298 [Q09LZ8-1]
GeneIDi78933
KEGGimmu:78933
UCSCiuc008udc.2 mouse [Q09LZ8-1]
uc008udd.2 mouse [Q09LZ8-2]

Organism-specific databases

CTDi84871
MGIiMGI:1918244 Agbl4

Phylogenomic databases

eggNOGiKOG3641 Eukaryota
COG2866 LUCA
GeneTreeiENSGT00550000074405
HOGENOMiHOG000231164
HOVERGENiHBG107588
InParanoidiQ09LZ8
OMAiKWQRIPR
OrthoDBiEOG091G0FXK
PhylomeDBiQ09LZ8
TreeFamiTF333009

Miscellaneous databases

ChiTaRSiAgbl4 mouse
PROiPR:Q09LZ8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000061298 Expressed in 83 organ(s), highest expression level in bone marrow
ExpressionAtlasiQ09LZ8 baseline and differential
GenevisibleiQ09LZ8 MM

Family and domain databases

InterProiView protein in InterPro
IPR000834 Peptidase_M14
PfamiView protein in Pfam
PF00246 Peptidase_M14, 1 hit
SMARTiView protein in SMART
SM00631 Zn_pept, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCBPC6_MOUSE
AccessioniPrimary (citable) accession number: Q09LZ8
Secondary accession number(s): A2A979
, A6PWC5, A6PWC6, Q09LZ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 17, 2006
Last modified: September 12, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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