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UniProtKB - Q09969 (BNIP3_CAEEL)
Protein
NIP3 homolog
Gene
dct-1
Organism
Caenorhabditis elegans
Status
Functioni
Initiates apoptosis in a BH3-independent mechanism possibly by recruiting ced-3 to mitochondria and other cytoplasmic membranes (PubMed:11114722).
Has a role in lifespan and tumor growth (PubMed:16380712, PubMed:17934462).
Required for the induction of mitophagy under stress conditions (PubMed:25896323).
4 PublicationsGO - Molecular functioni
- protease binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- apoptotic process Source: UniProtKB
- autophagy of mitochondrion Source: WormBase
- determination of adult lifespan Source: UniProtKB
- mitochondrial outer membrane permeabilization Source: GO_Central
- positive regulation of apoptotic process Source: InterPro
- positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis Source: WormBase
- regulation of cell cycle Source: UniProtKB
- regulation of programmed cell death Source: GO_Central
Keywordsi
Biological process | Apoptosis, Autophagy |
Protein family/group databases
TCDBi | 1.A.20.2.1, the bcl2/adenovirus e1b-interacting protein 3 (bnip3) family |
Names & Taxonomyi
Protein namesi | Recommended name: NIP3 homologShort name: CeBNIP3 Alternative name(s): Daf-16/FOXO controlled germline tumor affecting-1Imported |
Gene namesi | Name:dct-1Imported ORF Names:C14F5.1Imported |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | C14F5.1a ; CE47388 ; WBGene00015776 ; dct-1 C14F5.1b ; CE47263 ; WBGene00015776 ; dct-1 |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane 1 Publication; Single-pass membrane protein Curated
Mitochondrion
- mitochondrial membrane Source: UniProtKB
- mitochondrial outer membrane Source: WormBase
Nucleus
- nucleus Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
- protein-containing complex Source: WormBase
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 189 – 209 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Disruption phenotypei
RNAi-mediated knockdown causes an increased sensitivity to stress and inhibits mitophagy which leads to an accumulation of defective mitochondria that have increased mass, altered network morphology, decreased ATP levels, increased reactive oxygen species (ROS) generation, membrane depolarization, increased oxygen consumption and increased cytoplasmic Ca2+ that intensifies under stress conditions. RNAi-mediated knockdown in daf-2, isp-1, or clk-1 mutant backgrounds suppresses their increased lifespan phenotype.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064963 | 1 – 221 | NIP3 homologAdd BLAST | 221 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 26 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Post-translational modificationi
Ubiquitinated and degraded by the proteasome (PubMed:11114722). Under oxidative stress conditions, ubiquitinated at Lys-26 in a pink-1 dependent manner. Colocalizes with pdr-1 and may be ubiquitinated by it (PubMed:25896323).2 Publications
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q09969 |
PeptideAtlasi | Q09969 |
PTM databases
iPTMneti | Q09969 |
Expressioni
Tissue specificityi
Expressed in all somatic tissues including neurons, pharynx, intestine, body wall muscles and vulva muscles.1 Publication
Developmental stagei
Expressed in the embryo and throughout all stages of development to adulthood.1 Publication
Gene expression databases
Bgeei | WBGene00015776, Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues |
Interactioni
Subunit structurei
GO - Molecular functioni
- protease binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 45977, 5 interactors |
DIPi | DIP-26299N |
STRINGi | 6239.C14F5.1a |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 24 – 55 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 189 – 209 | Required for initiation of apoptosisAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 25 – 55 | Polar residuesSequence analysisAdd BLAST | 31 |
Sequence similaritiesi
Belongs to the NIP3 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502SF13, Eukaryota |
GeneTreei | ENSGT00390000013415 |
HOGENOMi | CLU_1251645_0_0_1 |
InParanoidi | Q09969 |
OMAi | MSESWVE |
OrthoDBi | 1250566at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR010548, BNIP3 |
PANTHERi | PTHR15186, PTHR15186, 1 hit |
Pfami | View protein in Pfam PF06553, BNIP3, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform a (identifier: Q09969-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSFLEFAKP KMLDIKRKIN FASGEKTDES VQPQQQTEQS SAQQTTPSAK
60 70 80 90 100
AVSNPFITPL TESTPGMSES WVELAPSRTS LCSSVDINMV IIDEKDKDSR
110 120 130 140 150
LSPVSIAQSP HVEFESLEQV KYKLVREMLP PGKNTDWIWD WSSRPENTPP
160 170 180 190 200
KTVRMVQYGS NLTTPPNSPE PELYQYLPCE SDSLFNVRVV FGFLVTNIFS
210 220
FVVGAAVGFA VCRKLIKHHR Q
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037301 | 66 – 68 | Missing in isoform b. Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF133832 mRNA Translation: AAD32265.1 FO080456 Genomic DNA Translation: CCD63843.2 FO080456 Genomic DNA Translation: CCD63844.2 AF080583 mRNA Translation: AAC31574.1 |
PIRi | T43338 |
RefSeqi | NP_509339.4, NM_076938.4 [Q09969-1] NP_509340.2, NM_076939.3 [Q09969-2] |
Genome annotation databases
EnsemblMetazoai | C14F5.1a.1; C14F5.1a.1; WBGene00015776 [Q09969-1] C14F5.1b.1; C14F5.1b.1; WBGene00015776 [Q09969-2] |
GeneIDi | 181053 |
KEGGi | cel:CELE_C14F5.1 |
UCSCi | C14F5.1a, c. elegans |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF133832 mRNA Translation: AAD32265.1 FO080456 Genomic DNA Translation: CCD63843.2 FO080456 Genomic DNA Translation: CCD63844.2 AF080583 mRNA Translation: AAC31574.1 |
PIRi | T43338 |
RefSeqi | NP_509339.4, NM_076938.4 [Q09969-1] NP_509340.2, NM_076939.3 [Q09969-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 45977, 5 interactors |
DIPi | DIP-26299N |
STRINGi | 6239.C14F5.1a |
Protein family/group databases
TCDBi | 1.A.20.2.1, the bcl2/adenovirus e1b-interacting protein 3 (bnip3) family |
PTM databases
iPTMneti | Q09969 |
Proteomic databases
PaxDbi | Q09969 |
PeptideAtlasi | Q09969 |
Genome annotation databases
EnsemblMetazoai | C14F5.1a.1; C14F5.1a.1; WBGene00015776 [Q09969-1] C14F5.1b.1; C14F5.1b.1; WBGene00015776 [Q09969-2] |
GeneIDi | 181053 |
KEGGi | cel:CELE_C14F5.1 |
UCSCi | C14F5.1a, c. elegans |
Organism-specific databases
CTDi | 181053 |
WormBasei | C14F5.1a ; CE47388 ; WBGene00015776 ; dct-1 C14F5.1b ; CE47263 ; WBGene00015776 ; dct-1 |
Phylogenomic databases
eggNOGi | ENOG502SF13, Eukaryota |
GeneTreei | ENSGT00390000013415 |
HOGENOMi | CLU_1251645_0_0_1 |
InParanoidi | Q09969 |
OMAi | MSESWVE |
OrthoDBi | 1250566at2759 |
Miscellaneous databases
PROi | PR:Q09969 |
Gene expression databases
Bgeei | WBGene00015776, Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR010548, BNIP3 |
PANTHERi | PTHR15186, PTHR15186, 1 hit |
Pfami | View protein in Pfam PF06553, BNIP3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BNIP3_CAEEL | |
Accessioni | Q09969Primary (citable) accession number: Q09969 Secondary accession number(s): Q95QW5, Q9UB15 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | October 3, 2012 | |
Last modified: | June 2, 2021 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families