Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent DNA helicase hus2/rqh1

Gene

rqh1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a role in the repair of UV-induced DNA damage in G2 via recombination-mediated repair. Also has a role in the repair of infrared-induced double DNA strand breaks. Exhibits an ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi555 – 562ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: PomBase
  • ATP-dependent 3'-5' DNA helicase activity Source: PomBase
  • DNA binding Source: PomBase
  • four-way junction helicase activity Source: GO_Central

GO - Biological processi

  • cellular response to DNA damage stimulus Source: PomBase
  • DNA duplex unwinding Source: PomBase
  • double-strand break repair via homologous recombination Source: PomBase
  • intra-S DNA damage checkpoint Source: PomBase
  • maintenance of rDNA Source: PomBase
  • mitotic recombination-dependent replication fork processing Source: PomBase
  • postreplication repair Source: PomBase
  • recombinational repair Source: PomBase
  • regulation of mitotic recombination involved in replication fork processing Source: PomBase
  • replication fork processing Source: PomBase
  • resolution of mitotic recombination intermediates Source: PomBase
  • UV-damage excision repair Source: PomBase

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-3108214 SUMOylation of DNA damage response and repair proteins

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase hus2/rqh1 (EC:3.6.4.12)
Gene namesi
Name:rqh1
Synonyms:hus2, rad12, rec9
ORF Names:SPAC2G11.12
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC2G11.12
PomBaseiSPAC2G11.12 rqh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi543T → I: Loss of helicase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002050581 – 1328ATP-dependent DNA helicase hus2/rqh1Add BLAST1328

Proteomic databases

MaxQBiQ09811
PaxDbiQ09811
PRIDEiQ09811

PTM databases

iPTMnetiQ09811

Interactioni

Subunit structurei

Interacts with top3.1 Publication

Protein-protein interaction databases

BioGridi278117, 88 interactors
DIPiDIP-61056N
IntActiQ09811, 1 interactor
STRINGi4896.SPAC2G11.12.1

Structurei

3D structure databases

ProteinModelPortaliQ09811
SMRiQ09811
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini528 – 707Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST180
Domaini728 – 876Helicase C-terminalPROSITE-ProRule annotationAdd BLAST149
Domaini1115 – 1195HRDCPROSITE-ProRule annotationAdd BLAST81

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi651 – 654DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi225 – 236Poly-AsnAdd BLAST12
Compositional biasi321 – 332Poly-AsnAdd BLAST12

Sequence similaritiesi

Belongs to the helicase family. RecQ subfamily.Curated

Phylogenomic databases

InParanoidiQ09811
KOiK10901
OrthoDBiEOG092C0HS8
PhylomeDBiQ09811

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR004589 DNA_helicase_ATP-dep_RecQ
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR010997 HRDC-like_sf
IPR002121 HRDC_dom
IPR027417 P-loop_NTPase
IPR032284 RecQ_Zn-bd
IPR018982 RQC_domain
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF00570 HRDC, 1 hit
PF16124 RecQ_Zn_bind, 1 hit
PF09382 RQC, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00341 HRDC, 1 hit
SM00956 RQC, 1 hit
SUPFAMiSSF47819 SSF47819, 1 hit
SSF52540 SSF52540, 3 hits
TIGRFAMsiTIGR00614 recQ_fam, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50967 HRDC, 1 hit

Sequencei

Sequence statusi: Complete.

Q09811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTKTNLNR HLDWFFRESP QKIENVTSPI KTLDFVKVKV SSSDIVVKDS
60 70 80 90 100
IPHKSKNVFD DFDDGYAIDL TEEHQSSSLN NLKWKDVEGP NILKPIKKIA
110 120 130 140 150
VPASESEEDF DDVDEEMLRA AEMEVFQSCQ PLAVNTADTT VSHSTSSSNV
160 170 180 190 200
PRSLNKIHDP SRFIKDNDVE NRIHVSSASK VASISNTSKP NPIVSENPIS
210 220 230 240 250
ATSVSIEIPI KPKELSNNLP FPRLNNNNTN NNNDNNAIEK RDSASPTPSS
260 270 280 290 300
VSSQISIDFS TWPHQNLLQY LDILRDEKSE ISDRIIEVME RYPFSSRFKE
310 320 330 340 350
WIPKRDILSQ KISSVLEVLS NNNNSNNNNG NNGTVPNAKT FFTPPSSITQ
360 370 380 390 400
QVPFPSTIIP ESTVKENSTR PYVNSHLVAN DKITATPFHS EAVVSPLQSN
410 420 430 440 450
IRNSDIAEFD EFDIDDADFT FNTTDPINDE SGASSDVVVI DDEEDDIENR
460 470 480 490 500
PLNQALKASK AAVSNASLLQ SSSLDRPLLG EMKDKNHKVL MPSLDDPMLS
510 520 530 540 550
YPWSKEVLGC LKHKFHLKGF RKNQLEAING TLSGKDVFIL MPTGGGKSLC
560 570 580 590 600
YQLPAVIEGG ASRGVTLVIS PLLSLMQDQL DHLRKLNIPS LPLSGEQPAD
610 620 630 640 650
ERRQVISFLM AKNVLVKLLY VTPEGLASNG AITRVLKSLY ERKLLARIVI
660 670 680 690 700
DEAHCVSHWG HDFRPDYKQL GLLRDRYQGI PFMALTATAN EIVKKDIINT
710 720 730 740 750
LRMENCLELK SSFNRPNLFY EIKPKKDLYT ELYRFISNGH LHESGIIYCL
760 770 780 790 800
SRTSCEQVAA KLRNDYGLKA WHYHAGLEKV ERQRIQNEWQ SGSYKIIVAT
810 820 830 840 850
IAFGMGVDKG DVRFVIHHSF PKSLEGYYQE TGRAGRDGKP AHCIMFYSYK
860 870 880 890 900
DHVTFQKLIM SGDGDAETKE RQRQMLRQVI QFCENKTDCR RKQVLAYFGE
910 920 930 940 950
NFDKVHCRKG CDICCEEATY IKQDMTEFSL QAIKLLKSIS GKATLLQLMD
960 970 980 990 1000
IFRGSKSAKI VENGWDRLEG AGVGKLLNRG DSERLFHHLV SEGVFVEKVE
1010 1020 1030 1040 1050
ANRRGFVSAY VVPGRQTIIN SVLAGKRRII LDVKESSSKP DTSSRSLSRS
1060 1070 1080 1090 1100
KTLPALREYQ LKSTTASVDC SIGTREVDEI YDSQMPPVKP SLIHSRNKID
1110 1120 1130 1140 1150
LEELSGQKFM SEYEIDVMTR CLKDLKLLRS NLMAIDDSRV SSYFTDSVLL
1160 1170 1180 1190 1200
SMAKKLPRNV KELKEIHGVS NEKAVNLGPK FLQVIQKFID EKEQNLEGTE
1210 1220 1230 1240 1250
LDPSLQSLDT DYPIDTNALS LDHEQGFSDD SDSVYEPSSP IEEGDEEVDG
1260 1270 1280 1290 1300
QRKDILNFMN SQSLTQTGSV PKRKSTSYTR PSKSYRHKRG STSYSRKRKY
1310 1320
STSQKDSRKT SKSANTSFIH PMVKQNYR
Length:1,328
Mass (Da):149,646
Last modified:November 1, 1995 - v1
Checksum:i13A9A5B94E5A9BD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09426 Genomic DNA Translation: CAA70577.1
CU329670 Genomic DNA Translation: CAA91177.1
PIRiS62467
RefSeqiNP_593092.1, NM_001018490.2

Genome annotation databases

EnsemblFungiiSPAC2G11.12.1; SPAC2G11.12.1:pep; SPAC2G11.12
GeneIDi2541620
KEGGispo:SPAC2G11.12

Similar proteinsi

Entry informationi

Entry nameiHUS2_SCHPO
AccessioniPrimary (citable) accession number: Q09811
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health