UniProtKB - Q09163 (DLK1_MOUSE)
Protein delta homolog 1
Dlk1
Functioni
May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.
2 PublicationsGO - Molecular functioni
- calcium ion binding Source: InterPro
GO - Biological processi
- bone mineralization Source: MGI
- cell differentiation Source: MGI
- embryonic skeletal system development Source: MGI
- multicellular organism growth Source: MGI
- negative regulation of fat cell differentiation Source: HGNC
- negative regulation of Notch signaling pathway Source: MGI
- negative regulation of ossification Source: MGI
- negative regulation of osteoblast differentiation Source: MGI
- negative regulation of vascular endothelial cell proliferation Source: BHF-UCL
- ossification Source: MGI
- osteoblast differentiation Source: MGI
- positive regulation of bone resorption Source: MGI
- positive regulation of cytokine production Source: MGI
- positive regulation of osteoclast differentiation Source: MGI
- post-embryonic development Source: MGI
- regulation of bone remodeling Source: MGI
- regulation of gene expression Source: MGI
Names & Taxonomyi
Protein namesi | Recommended name: Protein delta homolog 1Short name: DLK-1 Alternative name(s): Adipocyte differentiation inhibitor protein Preadipocyte factor 1 Short name: Pref-1 Cleaved into the following chain: |
Gene namesi | Name:Dlk1 Synonyms:Dlk, Pref1, Scp-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:94900, Dlk1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
Extracellular region or secreted
- extracellular space Source: MGI
Plasma Membrane
- external side of plasma membrane Source: MGI
- plasma membrane Source: Reactome
Other locations
- cytoplasm Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 24 – 305 | ExtracellularSequence analysisAdd BLAST | 282 | |
Transmembranei | 306 – 329 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 330 – 385 | CytoplasmicSequence analysisAdd BLAST | 56 |
Keywords - Cellular componenti
Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000007520 | 24 – 385 | Protein delta homolog 1Add BLAST | 362 | |
ChainiPRO_0000007521 | 24 – 305 | Fetal antigen 1Add BLAST | 282 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 26 ↔ 37 | PROSITE-ProRule annotation | ||
Disulfide bondi | 30 ↔ 43 | PROSITE-ProRule annotation | ||
Disulfide bondi | 45 ↔ 54 | PROSITE-ProRule annotation | ||
Disulfide bondi | 57 ↔ 68 | PROSITE-ProRule annotation | ||
Disulfide bondi | 63 ↔ 74 | PROSITE-ProRule annotation | ||
Disulfide bondi | 76 ↔ 85 | PROSITE-ProRule annotation | ||
Disulfide bondi | 92 ↔ 103 | PROSITE-ProRule annotation | ||
GlycosylationiCAR_000160 | 94 | O-linked (GalNAc...) serine1 Publication | 1 | |
Disulfide bondi | 97 ↔ 113 | PROSITE-ProRule annotation | ||
GlycosylationiCAR_000183 | 100 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 115 ↔ 124 | PROSITE-ProRule annotation | ||
Disulfide bondi | 131 ↔ 144 | PROSITE-ProRule annotation | ||
Disulfide bondi | 138 ↔ 156 | PROSITE-ProRule annotation | ||
Disulfide bondi | 158 ↔ 167 | PROSITE-ProRule annotation | ||
Glycosylationi | 165 | N-linked (GlcNAc...) asparagine; atypical; partial1 Publication | 1 | |
Glycosylationi | 174 | N-linked (GlcNAc...) asparagine; atypical1 Publication | 1 | |
Disulfide bondi | 176 ↔ 187 | PROSITE-ProRule annotation | ||
Disulfide bondi | 181 ↔ 196 | PROSITE-ProRule annotation | ||
Disulfide bondi | 198 ↔ 207 | PROSITE-ProRule annotation | ||
Disulfide bondi | 214 ↔ 225 | PROSITE-ProRule annotation | ||
GlycosylationiCAR_000161 | 216 | O-linked (GalNAc...) serine1 Publication | 1 | |
Disulfide bondi | 219 ↔ 235 | PROSITE-ProRule annotation | ||
GlycosylationiCAR_000162 | 224 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Disulfide bondi | 237 ↔ 246 | PROSITE-ProRule annotation | ||
Glycosylationi | 258 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 267 | O-linked (GalNAc...) threonine; partial1 Publication | 1 | |
Glycosylationi | 271 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 295 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | Q09163 |
PaxDbi | Q09163 |
PRIDEi | Q09163 |
ProteomicsDBi | 279427 [Q09163-1] 279428 [Q09163-2] 279429 [Q09163-3] 279430 [Q09163-4] 279431 [Q09163-5] 279432 [Q09163-6] |
PTM databases
GlyConnecti | 153, 9 N-Linked glycans (1 site), 4 O-Linked glycans (3 sites) |
GlyGeni | Q09163, 11 sites, 18 N-linked glycans (3 sites), 6 O-linked glycans (7 sites) |
iPTMneti | Q09163 |
PhosphoSitePlusi | Q09163 |
Expressioni
Tissue specificityi
Developmental stagei
Interactioni
Subunit structurei
Monomer.
Interacts with SH3RF2 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 199231, 1 interactor |
DIPi | DIP-6010N |
IntActi | Q09163, 1 interactor |
STRINGi | 10090.ENSMUSP00000105470 |
Miscellaneous databases
RNActi | Q09163, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 55 | EGF-like 1PROSITE-ProRule annotationAdd BLAST | 32 | |
Domaini | 53 – 86 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 88 – 125 | EGF-like 3PROSITE-ProRule annotationAdd BLAST | 38 | |
Domaini | 127 – 168 | EGF-like 4PROSITE-ProRule annotationAdd BLAST | 42 | |
Domaini | 172 – 208 | EGF-like 5PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 210 – 247 | EGF-like 6PROSITE-ProRule annotationAdd BLAST | 38 |
Keywords - Domaini
EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1217, Eukaryota |
InParanoidi | Q09163 |
OrthoDBi | 619882at2759 |
PhylomeDBi | Q09163 |
Family and domain databases
InterProi | View protein in InterPro IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site |
Pfami | View protein in Pfam PF00008, EGF, 4 hits |
SMARTi | View protein in SMART SM00181, EGF, 6 hits SM00179, EGF_CA, 4 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS00022, EGF_1, 5 hits PS01186, EGF_2, 6 hits PS50026, EGF_3, 6 hits |
s (6+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE
60 70 80 90 100
GPLCDKCVTA PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN
110 120 130 140 150
GTCVDLEKGQ YECSCTPGFS GKDCQHKAGP CVINGSPCQH GGACVDDEGQ
160 170 180 190 200
ASHASCLCPP GFSGNFCEIV AATNSCTPNP CENDGVCTDI GGDFRCRCPA
210 220 230 240 250
GFVDKTCSRP VSNCASGPCQ NGGTCLQHTQ VSFECLCKPP FMGPTCAKKR
260 270 280 290 300
GASPVQVTHL PSGYGLTYRL TPGVHELPVQ QPEQHILKVS MKELNKSTPL
310 320 330 340 350
LTEGQAICFT ILGVLTSLVV LGTVAIVFLN KCETWVSNLR YNHTFRKKKN
360 370 380
LLLQYNSGEE LAVNIIFPEK IDMTTFNKEA GDEEI
The sequence of this isoform differs from the canonical sequence as follows:
231-305: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
211-305: Missing.
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ925U3 | Q925U3_MOUSE | Delta-like | Dlk1 dlk | 385 | Annotation score: | ||
G3UWU8 | G3UWU8_MOUSE | Protein delta homolog 1 | Dlk1 | 310 | Annotation score: | ||
B4YB46 | B4YB46_MOUSE | Dlk1 | Dlk1 | 312 | Annotation score: | ||
B4YB48 | B4YB48_MOUSE | Dlk1 | Dlk1 | 334 | Annotation score: | ||
Q9DAU5 | Q9DAU5_MOUSE | Protein delta homolog 1 | Dlk1 | 290 | Annotation score: | ||
E9PVN3 | E9PVN3_MOUSE | Protein delta homolog 1 | Dlk1 | 292 | Annotation score: | ||
G3UX30 | G3UX30_MOUSE | Protein delta homolog 1 | Dlk1 | 185 | Annotation score: | ||
G3UZ17 | G3UZ17_MOUSE | Protein delta homolog 1 | Dlk1 | 122 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 250 | R → P in AAA37175 (PubMed:8500166).Curated | 1 | |
Sequence conflicti | 320 – 385 | VLGTV…GDEEI → CWAPWPSSFSTSAKPGCPTC ATTTCFARRRTSCCSITAAR SWRSISSSPRRLT in AAA37175 (PubMed:8500166).CuratedAdd BLAST | 66 | |
Sequence conflicti | 344 – 345 | TF → ML in BAA04121 (Ref. 4) Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 347 | Missing 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001379 | 211 – 305 | Missing in isoform D2. CuratedAdd BLAST | 95 | |
Alternative sequenceiVSP_001378 | 211 – 303 | Missing in isoform D. CuratedAdd BLAST | 93 | |
Alternative sequenceiVSP_001382 | 231 – 305 | Missing in isoform C2. CuratedAdd BLAST | 75 | |
Alternative sequenceiVSP_001381 | 231 – 303 | Missing in isoform C. CuratedAdd BLAST | 73 | |
Alternative sequenceiVSP_001380 | 231 – 281 | Missing in isoform B. CuratedAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z12171 mRNA Translation: CAA78162.1 U15980 mRNA Translation: AAB60495.1 L12721 mRNA Translation: AAA37175.1 S71340 Genomic DNA No translation available. D16847 mRNA Translation: BAA04121.1 |
CCDSi | CCDS26168.1 [Q09163-1] CCDS49173.1 [Q09163-6] CCDS49174.1 [Q09163-2] CCDS56863.1 [Q09163-3] |
PIRi | A54785 S53718 |
RefSeqi | NP_034182.2, NM_010052.5 |
Genome annotation databases
GeneIDi | 13386 |
KEGGi | mmu:13386 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z12171 mRNA Translation: CAA78162.1 U15980 mRNA Translation: AAB60495.1 L12721 mRNA Translation: AAA37175.1 S71340 Genomic DNA No translation available. D16847 mRNA Translation: BAA04121.1 |
CCDSi | CCDS26168.1 [Q09163-1] CCDS49173.1 [Q09163-6] CCDS49174.1 [Q09163-2] CCDS56863.1 [Q09163-3] |
PIRi | A54785 S53718 |
RefSeqi | NP_034182.2, NM_010052.5 |
3D structure databases
SMRi | Q09163 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199231, 1 interactor |
DIPi | DIP-6010N |
IntActi | Q09163, 1 interactor |
STRINGi | 10090.ENSMUSP00000105470 |
PTM databases
GlyConnecti | 153, 9 N-Linked glycans (1 site), 4 O-Linked glycans (3 sites) |
GlyGeni | Q09163, 11 sites, 18 N-linked glycans (3 sites), 6 O-linked glycans (7 sites) |
iPTMneti | Q09163 |
PhosphoSitePlusi | Q09163 |
Proteomic databases
jPOSTi | Q09163 |
PaxDbi | Q09163 |
PRIDEi | Q09163 |
ProteomicsDBi | 279427 [Q09163-1] 279428 [Q09163-2] 279429 [Q09163-3] 279430 [Q09163-4] 279431 [Q09163-5] 279432 [Q09163-6] |
Protocols and materials databases
DNASUi | 13386 |
Genome annotation databases
GeneIDi | 13386 |
KEGGi | mmu:13386 |
Organism-specific databases
CTDi | 8788 |
MGIi | MGI:94900, Dlk1 |
Phylogenomic databases
eggNOGi | KOG1217, Eukaryota |
InParanoidi | Q09163 |
OrthoDBi | 619882at2759 |
PhylomeDBi | Q09163 |
Miscellaneous databases
BioGRID-ORCSi | 13386, 1 hit in 64 CRISPR screens |
ChiTaRSi | Dlk1, mouse |
PROi | PR:Q09163 |
RNActi | Q09163, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site |
Pfami | View protein in Pfam PF00008, EGF, 4 hits |
SMARTi | View protein in SMART SM00181, EGF, 6 hits SM00179, EGF_CA, 4 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS00022, EGF_1, 5 hits PS01186, EGF_2, 6 hits PS50026, EGF_3, 6 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | DLK1_MOUSE | |
Accessioni | Q09163Primary (citable) accession number: Q09163 Secondary accession number(s): Q07645, Q62208 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot