UniProtKB - Q09137 (AAPK2_RAT)
5'-AMP-activated protein kinase catalytic subunit alpha-2
Prkaa2
Functioni
Catalytic activityi
- EC:2.7.11.12 Publications
- EC:2.7.11.12 Publications
- ATP + L-seryl-[acetyl-CoA carboxylase] = ADP + H+ + O-phospho-L-seryl-[acetyl-CoA carboxylase]1 PublicationEC:2.7.11.271 Publication
- ATP + L-seryl-[3-hydroxy-3-methylglutaryl-coenzyme A reductase] = ADP + H+ + O-phospho-L-seryl-[3-hydroxy-3-methylglutaryl-coenzyme A reductase]1 PublicationEC:2.7.11.311 Publication
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 45 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 139 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 22 – 30 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- [acetyl-CoA carboxylase] kinase activity Source: UniProtKB-EC
- [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity Source: UniProtKB-EC
- AMP-activated protein kinase activity Source: UniProtKB
- ATP binding Source: RGD
- chromatin binding Source: UniProtKB
- histone serine kinase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- protein-containing complex binding Source: RGD
- protein kinase activity Source: RGD
- protein-macromolecule adaptor activity Source: RGD
- protein serine/threonine/tyrosine kinase activity Source: RGD
- protein serine/threonine kinase activity Source: RGD
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- autophagy Source: UniProtKB-KW
- cellular response to calcium ion Source: ARUK-UCL
- cellular response to drug Source: RGD
- cellular response to glucose starvation Source: UniProtKB
- cellular response to glucose stimulus Source: ARUK-UCL
- cellular response to nutrient levels Source: UniProtKB
- cellular response to organonitrogen compound Source: RGD
- cellular response to oxidative stress Source: RGD
- cellular response to prostaglandin E stimulus Source: RGD
- cholesterol biosynthetic process Source: UniProtKB-KW
- energy homeostasis Source: UniProtKB
- fatty acid biosynthetic process Source: UniProtKB-KW
- fatty acid homeostasis Source: UniProtKB
- glucose homeostasis Source: UniProtKB
- intracellular signal transduction Source: GO_Central
- lipid biosynthetic process Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of gene expression Source: RGD
- negative regulation of TOR signaling Source: UniProtKB
- negative regulation of tubulin deacetylation Source: RGD
- positive regulation of autophagy Source: UniProtKB
- positive regulation of cellular protein localization Source: RGD
- positive regulation of glycolytic process Source: UniProtKB
- positive regulation of peptidyl-lysine acetylation Source: RGD
- protein phosphorylation Source: RGD
- regulation of circadian rhythm Source: UniProtKB
- regulation of gene expression Source: RGD
- regulation of lipid metabolic process Source: RGD
- regulation of macroautophagy Source: UniProtKB
- regulation of microtubule cytoskeleton organization Source: RGD
- regulation of stress granule assembly Source: RGD
- response to activity Source: RGD
- response to caffeine Source: RGD
- response to muscle activity Source: RGD
- rhythmic process Source: UniProtKB-KW
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 5301 2.7.11.31, 5301 |
Reactomei | R-RNO-1632852, Macroautophagy [Q09137-2] R-RNO-163680, AMPK inhibits chREBP transcriptional activation activity [Q09137-2] R-RNO-200425, Carnitine metabolism [Q09137-2] R-RNO-380972, Energy dependent regulation of mTOR by LKB1-AMPK [Q09137-2] R-RNO-5628897, TP53 Regulates Metabolic Genes [Q09137-2] R-RNO-6804756, Regulation of TP53 Activity through Phosphorylation [Q09137-2] |
SABIO-RKi | Q09137 |
Names & Taxonomyi
Protein namesi | Recommended name: 5'-AMP-activated protein kinase catalytic subunit alpha-2 (EC:2.7.11.12 Publications)Short name: AMPK subunit alpha-2 Alternative name(s): |
Gene namesi | Name:Prkaa2 Synonyms:Ampk, Ampk2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620893, Prkaa2 |
Subcellular locationi
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: RGD
Plasma Membrane
- apical plasma membrane Source: UniProtKB
Other locations
- axon Source: ARUK-UCL
- cytoplasm Source: UniProtKB
- cytoplasmic stress granule Source: RGD
- dendrite Source: ARUK-UCL
- neuronal cell body Source: ARUK-UCL
- nucleotide-activated protein kinase complex Source: RGD
- protein-containing complex Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085597 | 1 – 552 | 5'-AMP-activated protein kinase catalytic subunit alpha-2Add BLAST | 552 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 172 | Phosphothreonine; by LKB1 and CaMKK25 Publications | 1 | |
Modified residuei | 258 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 377 | PhosphoserineCombined sources | 1 | |
Modified residuei | 491 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q09137 |
PaxDbi | Q09137 |
PRIDEi | Q09137 |
PTM databases
iPTMneti | Q09137 |
PhosphoSitePlusi | Q09137 |
Expressioni
Tissue specificityi
Inductioni
Interactioni
Subunit structurei
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3).
Interacts with FNIP1 and FNIP2. Associates with internalized INSR complexes on Golgi/endosomal membranes; PRKAA2/AMPK2 together with ATIC and HACD3/PTPLAD1 is proposed to be part of a signaling network regulating INSR autophosphorylation and endocytosis (PubMed:25687571).
Interacts with DUSP29 (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 249382, 7 interactors |
CORUMi | Q09137 |
IntActi | Q09137, 1 interactor |
STRINGi | 10116.ENSRNOP00000010680 |
Chemistry databases
BindingDBi | Q09137 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 16 – 268 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 253 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 291 – 376 | AISBy similarityAdd BLAST | 86 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
InParanoidi | Q09137 |
OrthoDBi | 1127668at2759 |
PhylomeDBi | Q09137 |
Family and domain databases
CDDi | cd12200, AMPKA2_C, 1 hit cd14404, UBA_AID_AAPK2, 1 hit |
InterProi | View protein in InterPro IPR032270, AMPK_C IPR039148, AMPKA2_C IPR028375, KA1/Ssp2_C IPR011009, Kinase-like_dom_sf IPR028783, PRKAA2 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF16579, AdenylateSensor, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF103243, SSF103243, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEKQKHDGR VKIGHYVLGD TLGVGTFGKV KIGEHQLTGH KVAVKILNRQ
60 70 80 90 100
KIRSLDVVGK IKREIQNLKL FRHPHIIKLY QVISTPTDFF MVMEYVSGGE
110 120 130 140 150
LFDYICKHGR VEEVEARRLF QQILSAVDYC HRHMVVHRDL KPENVLLDAQ
160 170 180 190 200
MNAKIADFGL SNMMSDGEFL RTSCGSPNYA APEVISGRLY AGPEVDIWSC
210 220 230 240 250
GVILYALLCG TLPFDDEHVP TLFKKIRGGV FYIPEYLNRS IATLLMHMLQ
260 270 280 290 300
VDPLKRATIK DIREHEWFKQ DLPSYLFPED PSYDANVIDD EAVKEVCEKF
310 320 330 340 350
ECTESEVMNS LYSGDPQDQL AVAYHLIIDN RRIMNQASEF YLASSPPTGS
360 370 380 390 400
FMDDMAMHIP PGLKPHPERM PPLIADSPKA RCPLDALNTT KPKSLAVKKA
410 420 430 440 450
KWHLGIRSQS KPYDIMAEVY RAMKQLDFEW KVVNAYHLRV RRKNPVTGNY
460 470 480 490 500
VKMSLQLYLV DNRSYLLDFK SIDDEVVEQR SGSSTPQRSC SAAGLHRPRS
510 520 530 540 550
SVDSSTAENH SLSGSLTGSL TGSTLSSASP RLGSHTMDFF EMCASLITAL
AR
The sequence of this isoform differs from the canonical sequence as follows:
32-388: Missing.
392-552: Missing.
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V715 | G3V715_RAT | 5'-AMP-activated protein kinase cat... | Prkaa2 rCG_53426 | 552 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 355 | M → S in AAA85033 (PubMed:7718624).Curated | 1 | |
Sequence conflicti | 462 | N → D in AAA85033 (PubMed:7718624).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004949 | 32 – 388 | Missing in isoform Short. 1 PublicationAdd BLAST | 357 | |
Alternative sequenceiVSP_004950 | 392 – 552 | Missing in isoform Short. 1 PublicationAdd BLAST | 161 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z29486 mRNA Translation: CAA82620.1 U12149 mRNA Translation: AAA85033.1 |
PIRi | A53621 |
RefSeqi | NP_076481.1, NM_023991.1 [Q09137-1] |
Genome annotation databases
GeneIDi | 78975 |
KEGGi | rno:78975 |
UCSCi | RGD:620893, rat [Q09137-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z29486 mRNA Translation: CAA82620.1 U12149 mRNA Translation: AAA85033.1 |
PIRi | A53621 |
RefSeqi | NP_076481.1, NM_023991.1 [Q09137-1] |
3D structure databases
BMRBi | Q09137 |
SMRi | Q09137 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249382, 7 interactors |
CORUMi | Q09137 |
IntActi | Q09137, 1 interactor |
STRINGi | 10116.ENSRNOP00000010680 |
Chemistry databases
BindingDBi | Q09137 |
ChEMBLi | CHEMBL4637 |
PTM databases
iPTMneti | Q09137 |
PhosphoSitePlusi | Q09137 |
Proteomic databases
jPOSTi | Q09137 |
PaxDbi | Q09137 |
PRIDEi | Q09137 |
Genome annotation databases
GeneIDi | 78975 |
KEGGi | rno:78975 |
UCSCi | RGD:620893, rat [Q09137-1] |
Organism-specific databases
CTDi | 5563 |
RGDi | 620893, Prkaa2 |
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
InParanoidi | Q09137 |
OrthoDBi | 1127668at2759 |
PhylomeDBi | Q09137 |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 5301 2.7.11.31, 5301 |
Reactomei | R-RNO-1632852, Macroautophagy [Q09137-2] R-RNO-163680, AMPK inhibits chREBP transcriptional activation activity [Q09137-2] R-RNO-200425, Carnitine metabolism [Q09137-2] R-RNO-380972, Energy dependent regulation of mTOR by LKB1-AMPK [Q09137-2] R-RNO-5628897, TP53 Regulates Metabolic Genes [Q09137-2] R-RNO-6804756, Regulation of TP53 Activity through Phosphorylation [Q09137-2] |
SABIO-RKi | Q09137 |
Miscellaneous databases
PROi | PR:Q09137 |
Family and domain databases
CDDi | cd12200, AMPKA2_C, 1 hit cd14404, UBA_AID_AAPK2, 1 hit |
InterProi | View protein in InterPro IPR032270, AMPK_C IPR039148, AMPKA2_C IPR028375, KA1/Ssp2_C IPR011009, Kinase-like_dom_sf IPR028783, PRKAA2 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF16579, AdenylateSensor, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF103243, SSF103243, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AAPK2_RAT | |
Accessioni | Q09137Primary (citable) accession number: Q09137 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families