Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - Q09028 (RBBP4_HUMAN)

Protein

Histone-binding protein RBBP4

Gene

RBBP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.1 Publication

GO - Molecular functioni

  • histone binding Source: BHF-UCL
  • histone deacetylase activity Source: Reactome
  • histone deacetylase binding Source: BHF-UCL
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionChromatin regulator, Repressor
Biological processCell cycle, DNA replication, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1362277 Transcription of E2F targets under negative control by DREAM complex
R-HSA-1362300 Transcription of E2F targets under negative control by p107 (RBL1) and p130 (RBL2) in complex with HDAC1
R-HSA-1538133 G0 and Early G1
R-HSA-156711 Polo-like kinase mediated events
R-HSA-212300 PRC2 methylates histones and DNA
R-HSA-2559580 Oxidative Stress Induced Senescence
R-HSA-3214815 HDACs deacetylate histones
R-HSA-3214841 PKMTs methylate histone lysines
R-HSA-427389 ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression
R-HSA-539107 Activation of E2F1 target genes at G1/S
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-HSA-6804758 Regulation of TP53 Activity through Acetylation
R-HSA-69202 Cyclin E associated events during G1/S transition
R-HSA-69656 Cyclin A:Cdk2-associated events at S phase entry
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-8943724 Regulation of PTEN gene transcription
R-HSA-8953750 Transcriptional Regulation by E2F6
SignaLinkiQ09028
SIGNORiQ09028

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-binding protein RBBP4
Alternative name(s):
Chromatin assembly factor 1 subunit C
Short name:
CAF-1 subunit C
Chromatin assembly factor I p48 subunit
Short name:
CAF-I 48 kDa subunit
Short name:
CAF-I p48
Nucleosome-remodeling factor subunit RBAP48
Retinoblastoma-binding protein 4
Short name:
RBBP-4
Retinoblastoma-binding protein p48
Gene namesi
Name:RBBP4
Synonyms:RBAP48
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000162521.18
HGNCiHGNC:9887 RBBP4
MIMi602923 gene
neXtProtiNX_Q09028

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5928
OpenTargetsiENSG00000162521
PharmGKBiPA34251

Chemistry databases

ChEMBLiCHEMBL3137287

Polymorphism and mutation databases

BioMutaiRBBP4
DMDMi1172846

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000511862 – 425Histone-binding protein RBBP4Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei4N6-acetyllysine; alternateCombined sources1
Cross-linki4Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki4Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate
Modified residuei110PhosphoserineCombined sources1
Modified residuei160N6-acetyllysine; alternateBy similarity1
Cross-linki160Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei355PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ09028
MaxQBiQ09028
PaxDbiQ09028
PeptideAtlasiQ09028
PRIDEiQ09028
ProteomicsDBi58712
58713 [Q09028-2]
58714 [Q09028-3]
58715 [Q09028-4]

PTM databases

iPTMnetiQ09028
PhosphoSitePlusiQ09028
SwissPalmiQ09028

Expressioni

Gene expression databases

BgeeiENSG00000162521 Expressed in 209 organ(s), highest expression level in lung
CleanExiHS_RBBP4
ExpressionAtlasiQ09028 baseline and differential
GenevisibleiQ09028 HS

Organism-specific databases

HPAiCAB006264
HPA060710
HPA060724

Interactioni

Subunit structurei

Interacts with SUV39H1 and HDAC7 (By similarity). Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the chromatin assembly factor 1 (CAF-1) complex, which is composed of RBBP4, CHAF1B and CHAF1A. Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SUDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex. The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex). The NuRD complex may also interact with MBD3L1 and MBD3L2. Interacts with MTA1. Subunit of the PRC2/EED-EZH2 complex, which is composed of at least EED, EZH2, RBBP4, RBBP7 and SUZ12. The PRC2/EED-EZH2 complex may also associate with HDAC1. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SPEN/MINT. Interacts with BRCA1. Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with PHF6 (By similarity). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1 (By similarity).By similarity25 Publications

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi111863, 197 interactors
ComplexPortaliCPX-3321 SIN3A histone deacetylase complex
CPX-3322 SIN3B histone deacetylase complex
CPX-3323 SIN3A histone deacetylase complex, ES cell-specific variant
CPX-569 Chromatin assembly factor 1 complex
CPX-688 NuRF chromatin remodelling complex
CPX-880 MBD2/NuRD nucleosome remodeling and deacetylase complex
CPX-922 MBD3/NuRD nucleosome remodeling and deacetylase complex
CORUMiQ09028
DIPiDIP-33495N
IntActiQ09028, 122 interactors
MINTiQ09028
STRINGi9606.ENSP00000362592

Chemistry databases

BindingDBiQ09028

Structurei

Secondary structure

1425
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ09028
SMRiQ09028
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ09028

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati122 – 155WD 1Add BLAST34
Repeati175 – 206WD 2Add BLAST32
Repeati225 – 256WD 3Add BLAST32
Repeati271 – 302WD 4Add BLAST32
Repeati315 – 346WD 5Add BLAST32
Repeati372 – 403WD 6Add BLAST32

Sequence similaritiesi

Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0264 Eukaryota
ENOG410XNU9 LUCA
GeneTreeiENSGT00570000079069
HOVERGENiHBG053236
InParanoidiQ09028
KOiK10752
OMAiTNHLADF
OrthoDBiEOG091G0A20
PhylomeDBiQ09028
TreeFamiTF106485

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR022052 Histone-bd_RBBP4_N
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12265 CAF1C_H4-bd, 1 hit
PF00400 WD40, 5 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 5 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 9 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q09028-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MADKEAAFDD AVEERVINEE YKIWKKNTPF LYDLVMTHAL EWPSLTAQWL 
        60         70         80         90        100
PDVTRPEGKD FSIHRLVLGT HTSDEQNHLV IASVQLPNDD AQFDASHYDS 
       110        120        130        140        150
EKGEFGGFGS VSGKIEIEIK INHEGEVNRA RYMPQNPCII ATKTPSSDVL 
       160        170        180        190        200
VFDYTKHPSK PDPSGECNPD LRLRGHQKEG YGLSWNPNLS GHLLSASDDH 
       210        220        230        240        250
TICLWDISAV PKEGKVVDAK TIFTGHTAVV EDVSWHLLHE SLFGSVADDQ 
       260        270        280        290        300
KLMIWDTRSN NTSKPSHSVD AHTAEVNCLS FNPYSEFILA TGSADKTVAL 
       310        320        330        340        350
WDLRNLKLKL HSFESHKDEI FQVQWSPHNE TILASSGTDR RLNVWDLSKI 
       360        370        380        390        400
GEEQSPEDAE DGPPELLFIH GGHTAKISDF SWNPNEPWVI CSVSEDNIMQ 
       410        420 
VWQMAENIYN DEDPEGSVDP EGQGS
Length:425
Mass (Da):47,656
Last modified:January 23, 2007 - v3
Checksum:iB71E2D55A444C360
GO
Isoform 2 (identifier: Q09028-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     7-7: Missing.

Note: No experimental confirmation available.
Show »
Length:424
Mass (Da):47,585
Checksum:i52055B5C74BCF3D8
GO
Isoform 3 (identifier: Q09028-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     405-425: AENIYNDEDPEGSVDPEGQGS → ELVLDH

Note: No experimental confirmation available.
Show »
Length:410
Mass (Da):46,158
Checksum:i59B630D6055A70B2
GO
Isoform 4 (identifier: Q09028-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.

Note: No experimental confirmation available.
Show »
Length:390
Mass (Da):43,482
Checksum:iA61A43F6AC30C8CE
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YDK2H0YDK2_HUMAN
Histone-binding protein RBBP4
RBBP4
262Annotation score:
H0YEU5H0YEU5_HUMAN
Histone-binding protein RBBP4
RBBP4
167Annotation score:
H0YF10H0YF10_HUMAN
Histone-binding protein RBBP4
RBBP4
207Annotation score:
H0YCT5H0YCT5_HUMAN
Histone-binding protein RBBP4
RBBP4
180Annotation score:
C9JPP3C9JPP3_HUMAN
Histone-binding protein RBBP4
RBBP4
87Annotation score:
E9PNS2E9PNS2_HUMAN
Histone-binding protein RBBP4
RBBP4
62Annotation score:
E9PNS6E9PNS6_HUMAN
Histone-binding protein RBBP4
RBBP4
104Annotation score:
E9PND5E9PND5_HUMAN
Histone-binding protein RBBP4
RBBP4
68Annotation score:
E9PIC4E9PIC4_HUMAN
Histone-binding protein RBBP4
RBBP4
87Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61F → G in AAH15123 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0400871 – 35Missing in isoform 4. 1 PublicationAdd BLAST35
Alternative sequenceiVSP_0400887Missing in isoform 2. 1 Publication1
Alternative sequenceiVSP_040089405 – 425AENIY…EGQGS → ELVLDH in isoform 3. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74262 mRNA Translation: CAA52321.1
X71810 mRNA Translation: CAA50685.1
BT007309 mRNA Translation: AAP35973.1
AK299251 mRNA Translation: BAG61282.1
AK312571 mRNA Translation: BAG35466.1
AK222779 mRNA Translation: BAD96499.1
AC114489 Genomic DNA No translation available.
CH471059 Genomic DNA Translation: EAX07513.1
CH471059 Genomic DNA Translation: EAX07514.1
BC003092 mRNA Translation: AAH03092.1
BC015123 mRNA Translation: AAH15123.1
BC053904 mRNA Translation: AAH53904.1
BC075836 mRNA Translation: AAH75836.1
CCDSiCCDS366.1 [Q09028-1]
CCDS44105.1 [Q09028-2]
CCDS44106.1 [Q09028-4]
PIRiS36112
RefSeqiNP_001128727.1, NM_001135255.1 [Q09028-2]
NP_001128728.1, NM_001135256.1 [Q09028-4]
NP_005601.1, NM_005610.2 [Q09028-1]
UniGeneiHs.16003

Genome annotation databases

EnsembliENST00000373485; ENSP00000362584; ENSG00000162521 [Q09028-3]
ENST00000373493; ENSP00000362592; ENSG00000162521 [Q09028-1]
ENST00000414241; ENSP00000398242; ENSG00000162521 [Q09028-2]
ENST00000458695; ENSP00000396057; ENSG00000162521 [Q09028-4]
GeneIDi5928
KEGGihsa:5928
UCSCiuc001bvr.3 human [Q09028-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRBBP4_HUMAN
AccessioniPrimary (citable) accession number: Q09028
Secondary accession number(s): B2R6G9
, B4DRH0, D3DPQ3, P31149, Q53H02, Q96BV9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 204 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again