UniProtKB - Q08DY9 (CASP3_BOVIN)
Caspase-3
CASP3
Functioni
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity).
Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes (By similarity).
Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (By similarity).
By similarityCatalytic activityi
- Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.By similarity EC:3.4.22.56
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 121 | By similarity | 1 | |
Active sitei | 163 | By similarity | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: UniProtKB
- endopeptidase activity Source: UniProtKB
GO - Biological processi
- positive regulation of amyloid-beta formation Source: UniProtKB
- proteolysis Source: UniProtKB
- regulation of protein stability Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Apoptosis |
Protein family/group databases
MEROPSi | C14.003 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CASP3 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000261328 | 1 – 9 | By similarity | 9 | |
PropeptideiPRO_0000261329 | 10 – 28 | By similarityAdd BLAST | 19 | |
ChainiPRO_0000261330 | 29 – 175 | Caspase-3 subunit p17Add BLAST | 147 | |
ChainiPRO_0000261331 | 176 – 275 | Caspase-3 subunit p12Add BLAST | 100 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 11 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 26 | PhosphoserineBy similarity | 1 | |
Modified residuei | 163 | S-nitrosocysteine; in inhibited formBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, S-nitrosylation, ZymogenProteomic databases
PaxDbi | Q08DY9 |
PRIDEi | Q08DY9 |
Interactioni
Subunit structurei
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
Interacts with BIRC6/bruce.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000021099 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3573, Eukaryota |
InParanoidi | Q08DY9 |
OrthoDBi | 984395at2759 |
Family and domain databases
CDDi | cd00032, CASc, 1 hit |
InterProi | View protein in InterPro IPR015471, Casp3/7 IPR029030, Caspase-like_dom_sf IPR033139, Caspase_cys_AS IPR016129, Caspase_his_AS IPR002398, Pept_C14 IPR002138, Pept_C14_p10 IPR001309, Pept_C14_p20 IPR015917, Pept_C14A |
PANTHERi | PTHR10454, PTHR10454, 1 hit PTHR10454:SF31, PTHR10454:SF31, 1 hit |
PRINTSi | PR00376, IL1BCENZYME |
SMARTi | View protein in SMART SM00115, CASc, 1 hit |
SUPFAMi | SSF52129, SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS01122, CASPASE_CYS, 1 hit PS01121, CASPASE_HIS, 1 hit PS50207, CASPASE_P10, 1 hit PS50208, CASPASE_P20, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MENTENSVDS KSIKTSETKI LHGSKSMDSG ISLEESYKMD YPEMGLCIII
60 70 80 90 100
NNKNFHENTG MACRSGTDVD AANLRETFMN LKYEVRIKND LTCKEMLELM
110 120 130 140 150
SNVSKEDHSK RSSFICVLLS HGEEGIIFGT NGPVNLKKLA SFFRGDYCRS
160 170 180 190 200
LTGKPKLFII QACRGTELDC GIETDSGAED DMACQKIPVE ADFLYAYSTA
210 220 230 240 250
PGYFSWRNAK NGSWFIQALC EMLKKHAHRL ELMHILTRVN RKVAIEYESF
260 270
STDSAFHAKK QIPCIMSMLT KELYF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC123503 mRNA Translation: AAI23504.1 |
RefSeqi | NP_001071308.1, NM_001077840.1 |
Genome annotation databases
GeneIDi | 408016 |
KEGGi | bta:408016 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC123503 mRNA Translation: AAI23504.1 |
RefSeqi | NP_001071308.1, NM_001077840.1 |
3D structure databases
SMRi | Q08DY9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000021099 |
Protein family/group databases
MEROPSi | C14.003 |
Proteomic databases
PaxDbi | Q08DY9 |
PRIDEi | Q08DY9 |
Genome annotation databases
GeneIDi | 408016 |
KEGGi | bta:408016 |
Organism-specific databases
CTDi | 836 |
Phylogenomic databases
eggNOGi | KOG3573, Eukaryota |
InParanoidi | Q08DY9 |
OrthoDBi | 984395at2759 |
Family and domain databases
CDDi | cd00032, CASc, 1 hit |
InterProi | View protein in InterPro IPR015471, Casp3/7 IPR029030, Caspase-like_dom_sf IPR033139, Caspase_cys_AS IPR016129, Caspase_his_AS IPR002398, Pept_C14 IPR002138, Pept_C14_p10 IPR001309, Pept_C14_p20 IPR015917, Pept_C14A |
PANTHERi | PTHR10454, PTHR10454, 1 hit PTHR10454:SF31, PTHR10454:SF31, 1 hit |
PRINTSi | PR00376, IL1BCENZYME |
SMARTi | View protein in SMART SM00115, CASc, 1 hit |
SUPFAMi | SSF52129, SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS01122, CASPASE_CYS, 1 hit PS01121, CASPASE_HIS, 1 hit PS50207, CASPASE_P10, 1 hit PS50208, CASPASE_P20, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CASP3_BOVIN | |
Accessioni | Q08DY9Primary (citable) accession number: Q08DY9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | October 31, 2006 | |
Last modified: | February 23, 2022 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families