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Protein

Protein spire homolog 1

Gene

SPIRE1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:11747823, PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (PubMed:11747823). Required for asymmetric spindle positioning and asymmetric cell division during meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with FMN2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PubMed:26287480).3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein spire homolog 1
Short name:
Spir-1
Gene namesi
Name:SPIRE1Imported
Synonyms:KIAA1135, SPIR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000134278.14
HGNCiHGNC:30622 SPIRE1
MIMi609216 gene
neXtProtiNX_Q08AE8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi131I → K: Strongly reduces interaction with FMN2. 1 Publication1
Mutagenesisi134Y → K: Abolishes interaction with FMN2. 1 Publication1
Mutagenesisi138D → N: Abolishes interaction with FMN2. 2 Publications1
Mutagenesisi146E → A or K: Abolishes interaction with FMN2. 1 Publication1

Organism-specific databases

DisGeNETi56907
OpenTargetsiENSG00000134278
PharmGKBiPA134895885

Polymorphism and mutation databases

BioMutaiSPIRE1
DMDMi425906061

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003095692 – 756Protein spire homolog 1Add BLAST755

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei387PhosphoserineCombined sources1
Modified residuei406PhosphoserineBy similarity1
Modified residuei464PhosphoserineCombined sources1
Modified residuei465PhosphoserineCombined sources1
Modified residuei467PhosphoserineCombined sources1
Modified residuei509PhosphothreonineCombined sources1
Modified residuei678PhosphoserineCombined sources1
Modified residuei682PhosphoserineBy similarity1
Modified residuei735PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ08AE8
PaxDbiQ08AE8
PeptideAtlasiQ08AE8
PRIDEiQ08AE8
ProteomicsDBi58658
58659 [Q08AE8-2]
58660 [Q08AE8-3]
58661 [Q08AE8-4]

PTM databases

iPTMnetiQ08AE8
PhosphoSitePlusiQ08AE8

Expressioni

Gene expression databases

BgeeiENSG00000134278 Expressed in 204 organ(s), highest expression level in medial globus pallidus
CleanExiHS_SPIRE1
ExpressionAtlasiQ08AE8 baseline and differential
GenevisibleiQ08AE8 HS

Organism-specific databases

HPAiHPA040737
HPA040942

Interactioni

Subunit structurei

Interacts with FMN2.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121237, 10 interactors
DIPiDIP-42378N
IntActiQ08AE8, 6 interactors
MINTiQ08AE8
STRINGi9606.ENSP00000387266

Structurei

Secondary structure

1756
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ08AE8
SMRiQ08AE8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 231KINDPROSITE-ProRule annotationAdd BLAST192
Domaini305 – 323WH2 1Add BLAST19
Domaini369 – 386WH2 2Add BLAST18

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 138Important for interaction with FMN28
Regioni556 – 576Spir-boxAdd BLAST21

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili229 – 257Sequence analysisAdd BLAST29

Domaini

Binds to actin monomers via the WH2 domain.By similarity
The Spir-box targets binding to intracellular membrane structures.1 Publication

Sequence similaritiesi

Belongs to the spire family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IK9H Eukaryota
ENOG4111E0J LUCA
GeneTreeiENSGT00390000003058
HOGENOMiHOG000013039
HOVERGENiHBG058898
InParanoidiQ08AE8
KOiK02098
OMAiQVPAQRK
OrthoDBiEOG091G0EAQ
PhylomeDBiQ08AE8
TreeFamiTF326239

Family and domain databases

InterProiView protein in InterPro
IPR011019 KIND_dom
IPR029901 Spire
IPR029905 Spire1
IPR011011 Znf_FYVE_PHD
PANTHERiPTHR21345 PTHR21345, 1 hit
PTHR21345:SF3 PTHR21345:SF3, 1 hit
PfamiView protein in Pfam
PF16474 KIND, 1 hit
SMARTiView protein in SMART
SM00750 KIND, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS51377 KIND, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 11 Publication (identifier: Q08AE8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAQAAGPAGG GEPRTEAVGG EGPREPGAAG GAAGGSRDAL SLEEILRLYN
60 70 80 90 100
QPINEEQAWA VCYQCCGSLR AAARRRQPRH RVRSAAQIRV WRDGAVTLAP
110 120 130 140 150
AADDAGEPPP VAGKLGYSQC METEVIESLG IIIYKALDYG LKENEERELS
160 170 180 190 200
PPLEQLIDHM ANTVEADGSN DEGYEAAEEG LGDEDEKRKI SAIRSYRDVM
210 220 230 240 250
KLCAAHLPTE SDAPNHYQAV CRALFAETME LHTFLTKIKS AKENLKKIQE
260 270 280 290 300
MEKSDESSTD LEELKNADWA RFWVQVMRDL RNGVKLKKVQ ERQYNPLPIE
310 320 330 340 350
YQLTPYEMLM DDIRCKRYTL RKVMVNGDIP PRLKKSAHEI ILDFIRSRPP
360 370 380 390 400
LNPVSARKLK PTPPRPRSLH ERILEEIKAE RKLRPVSPEE IRRSRLAMRP
410 420 430 440 450
LSMSYSFDLS DVTTPESTKN LVESSMVNGG LTSQTKENGL STSQQVPAQR
460 470 480 490 500
KKLLRAPTLA ELDSSESEEE TLHKSTSSSS VSPSFPEEPV LEAVSTRKKP
510 520 530 540 550
PKFLPISSTP QPERRQPPQR RHSIEKETPT NVRQFLPPSR QSSRSLEEFC
560 570 580 590 600
YPVECLALTV EEVMHIRQVL VKAELEKYQQ YKDIYTALKK GKLCFCCRTR
610 620 630 640 650
RFSFFTWSYT CQFCKRPVCS QCCKKMRLPS KPYSTLPIFS LGPSALQRGE
660 670 680 690 700
SSMRSEKPST AHHRPLRSIA RFSSKSKSMD KSDEELQFPK ELMEDWSTME
710 720 730 740 750
VCVDCKKFIS EIISSSRRSL VLANKRARLK RKTQSFYMSS PGPSEYCPSE

RTISEI
Note: No experimental confirmation available.Curated
Length:756
Mass (Da):85,544
Last modified:November 28, 2012 - v3
Checksum:i1379B39C8CA9DDB3
GO
Isoform 5 (identifier: Q08AE8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-120: Missing.
     397-410: Missing.

Note: No experimental confirmation available. Derived from EST data.
Show »
Length:622
Mass (Da):71,568
Checksum:iD4DF3C072A295F96
GO
Isoform 22 Publications (identifier: Q08AE8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     397-410: Missing.

Show »
Length:742
Mass (Da):83,957
Checksum:iF76F168197958220
GO
Isoform 3 (identifier: Q08AE8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.
     397-410: Missing.

Show »
Length:583
Mass (Da):67,057
Checksum:i89163C062A16AB3D
GO
Isoform 4 (identifier: Q08AE8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.
     397-410: Missing.
     616-756: RPVCSQCCKK...CPSERTISEI → SDCLFNGTAHCQFYLG

Note: No experimental confirmation available.
Show »
Length:458
Mass (Da):52,747
Checksum:iBCE16535DEA4326C
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KNG6J3KNG6_HUMAN
Protein spire homolog 1
SPIRE1
545Annotation score:
C9JYR7C9JYR7_HUMAN
Protein spire homolog 1
SPIRE1
197Annotation score:
K7ENV1K7ENV1_HUMAN
Protein spire homolog 1
SPIRE1
147Annotation score:
K7EQR2K7EQR2_HUMAN
Protein spire homolog 1
SPIRE1
122Annotation score:
K7EMJ7K7EMJ7_HUMAN
Protein spire homolog 1
SPIRE1
122Annotation score:

Sequence cautioni

The sequence AAI15006 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI25207 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI25208 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA86449 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_058695249Q → P. Corresponds to variant dbSNP:rs1785296Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0379251 – 159Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST159
Alternative sequenceiVSP_0544641 – 120Missing in isoform 5. CuratedAdd BLAST120
Alternative sequenceiVSP_052595397 – 410Missing in isoform 2, isoform 3, isoform 4 and isoform 5. 4 PublicationsAdd BLAST14
Alternative sequenceiVSP_037926616 – 756RPVCS…TISEI → SDCLFNGTAHCQFYLG in isoform 4. 1 PublicationAdd BLAST141

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032961 mRNA Translation: BAA86449.2 Different initiation.
AK290180 mRNA Translation: BAF82869.1
AL833817 mRNA Translation: CAD38680.1
AP001028 Genomic DNA No translation available.
AP001029 Genomic DNA No translation available.
AP005482 Genomic DNA No translation available.
CH471113 Genomic DNA Translation: EAX01546.1
BC115005 mRNA Translation: AAI15006.1 Different initiation.
BC125206 mRNA Translation: AAI25207.1 Different initiation.
BC125207 mRNA Translation: AAI25208.1 Different initiation.
AJ277587 mRNA Translation: CAB96370.1
CCDSiCCDS32790.2 [Q08AE8-2]
CCDS45829.1 [Q08AE8-1]
CCDS45830.1 [Q08AE8-5]
RefSeqiNP_001122098.1, NM_001128626.1 [Q08AE8-1]
NP_001122099.1, NM_001128627.1 [Q08AE8-5]
NP_064533.3, NM_020148.2 [Q08AE8-2]
UniGeneiHs.515283

Genome annotation databases

EnsembliENST00000409402; ENSP00000387266; ENSG00000134278 [Q08AE8-1]
ENST00000410092; ENSP00000387226; ENSG00000134278 [Q08AE8-2]
ENST00000440472; ENSP00000404752; ENSG00000134278 [Q08AE8-4]
ENST00000453447; ENSP00000407050; ENSG00000134278 [Q08AE8-5]
GeneIDi56907
KEGGihsa:56907
UCSCiuc002kre.4 human [Q08AE8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032961 mRNA Translation: BAA86449.2 Different initiation.
AK290180 mRNA Translation: BAF82869.1
AL833817 mRNA Translation: CAD38680.1
AP001028 Genomic DNA No translation available.
AP001029 Genomic DNA No translation available.
AP005482 Genomic DNA No translation available.
CH471113 Genomic DNA Translation: EAX01546.1
BC115005 mRNA Translation: AAI15006.1 Different initiation.
BC125206 mRNA Translation: AAI25207.1 Different initiation.
BC125207 mRNA Translation: AAI25208.1 Different initiation.
AJ277587 mRNA Translation: CAB96370.1
CCDSiCCDS32790.2 [Q08AE8-2]
CCDS45829.1 [Q08AE8-1]
CCDS45830.1 [Q08AE8-5]
RefSeqiNP_001122098.1, NM_001128626.1 [Q08AE8-1]
NP_001122099.1, NM_001128627.1 [Q08AE8-5]
NP_064533.3, NM_020148.2 [Q08AE8-2]
UniGeneiHs.515283

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YLEX-ray1.80A36-236[»]
2YLFX-ray2.05A36-236[»]
3R7GX-ray2.20A20-237[»]
3RBWX-ray3.20A/B/C/D20-237[»]
ProteinModelPortaliQ08AE8
SMRiQ08AE8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121237, 10 interactors
DIPiDIP-42378N
IntActiQ08AE8, 6 interactors
MINTiQ08AE8
STRINGi9606.ENSP00000387266

PTM databases

iPTMnetiQ08AE8
PhosphoSitePlusiQ08AE8

Polymorphism and mutation databases

BioMutaiSPIRE1
DMDMi425906061

Proteomic databases

MaxQBiQ08AE8
PaxDbiQ08AE8
PeptideAtlasiQ08AE8
PRIDEiQ08AE8
ProteomicsDBi58658
58659 [Q08AE8-2]
58660 [Q08AE8-3]
58661 [Q08AE8-4]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000409402; ENSP00000387266; ENSG00000134278 [Q08AE8-1]
ENST00000410092; ENSP00000387226; ENSG00000134278 [Q08AE8-2]
ENST00000440472; ENSP00000404752; ENSG00000134278 [Q08AE8-4]
ENST00000453447; ENSP00000407050; ENSG00000134278 [Q08AE8-5]
GeneIDi56907
KEGGihsa:56907
UCSCiuc002kre.4 human [Q08AE8-1]

Organism-specific databases

CTDi56907
DisGeNETi56907
EuPathDBiHostDB:ENSG00000134278.14
GeneCardsiSPIRE1
HGNCiHGNC:30622 SPIRE1
HPAiHPA040737
HPA040942
MIMi609216 gene
neXtProtiNX_Q08AE8
OpenTargetsiENSG00000134278
PharmGKBiPA134895885
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IK9H Eukaryota
ENOG4111E0J LUCA
GeneTreeiENSGT00390000003058
HOGENOMiHOG000013039
HOVERGENiHBG058898
InParanoidiQ08AE8
KOiK02098
OMAiQVPAQRK
OrthoDBiEOG091G0EAQ
PhylomeDBiQ08AE8
TreeFamiTF326239

Miscellaneous databases

ChiTaRSiSPIRE1 human
GenomeRNAii56907
PROiPR:Q08AE8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134278 Expressed in 204 organ(s), highest expression level in medial globus pallidus
CleanExiHS_SPIRE1
ExpressionAtlasiQ08AE8 baseline and differential
GenevisibleiQ08AE8 HS

Family and domain databases

InterProiView protein in InterPro
IPR011019 KIND_dom
IPR029901 Spire
IPR029905 Spire1
IPR011011 Znf_FYVE_PHD
PANTHERiPTHR21345 PTHR21345, 1 hit
PTHR21345:SF3 PTHR21345:SF3, 1 hit
PfamiView protein in Pfam
PF16474 KIND, 1 hit
SMARTiView protein in SMART
SM00750 KIND, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS51377 KIND, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPIR1_HUMAN
AccessioniPrimary (citable) accession number: Q08AE8
Secondary accession number(s): A8K2B5
, J3KQ50, J3KQR5, Q1RMD4, Q8NDP1, Q9NQ71, Q9ULT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 28, 2012
Last modified: October 10, 2018
This is version 111 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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