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Entry version 142 (11 Dec 2019)
Sequence version 1 (01 Nov 1996)
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Protein

Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21

Gene

THI21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and also probably that of HMP to HMP-P.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20 (THI20), Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21 (THI21)
  3. Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20 (THI20), Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21 (THI21)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei64SubstrateBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • thiamine biosynthetic process Source: SGD
  • thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:MONOMER3O-81
YEAST:MONOMER3O-81

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00060;UER00137
UPA00060;UER00138

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21 (EC:2.7.1.49Curated, EC:2.7.4.7Curated)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:THI21
Ordered Locus Names:YPL258C
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XVI

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YPL258C

Saccharomyces Genome Database

More...
SGDi
S000006179 THI21

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001920421 – 551Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21Add BLAST551

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q08975

PRoteomics IDEntifications database

More...
PRIDEi
Q08975

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

By absence of thiamine.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
P399403EBI-30327,EBI-16219

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
35954, 52 interactors

Database of interacting proteins

More...
DIPi
DIP-5635N

Protein interaction database and analysis system

More...
IntActi
Q08975, 41 interactors

Molecular INTeraction database

More...
MINTi
Q08975

STRING: functional protein association networks

More...
STRINGi
4932.YPL258C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q08975 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q08975

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the N-terminal section; belongs to the ThiD family.Curated
In the C-terminal section; belongs to the thiaminase-2 family.Curated

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000225275

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q08975

KEGG Orthology (KO)

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KOi
K00877

Identification of Orthologs from Complete Genome Data

More...
OMAi
AHSVYGM

Family and domain databases

Conserved Domains Database

More...
CDDi
cd01169 HMPP_kinase, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.20.910.10, 1 hit
3.40.1190.20, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR016084 Haem_Oase-like_multi-hlx
IPR004399 HMP/HMP-P_kinase
IPR013749 PM/HMP-P_kinase-1
IPR029056 Ribokinase-like
IPR004305 Thiaminase-2/PQQC
IPR027574 Thiaminase_II

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF08543 Phos_pyr_kin, 1 hit
PF03070 TENA_THI-4, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48613 SSF48613, 1 hit
SSF53613 SSF53613, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00097 HMP-P_kinase, 1 hit
TIGR04306 salvage_TenA, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q08975-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTYSTVNINT PPPYLALASN EKLPTVLSIA GTDPSGGAGV EADVKTITAH
60 70 80 90 100
RCYAMTCITA LNAQTPVKVY SINNTPKEVV SQILDANLQD MKCDVIKTGM
110 120 130 140 150
LTTAAIEVLH EKLLQLGENR PKLVVDPVLV ATSGSSLAGK DIASLITEKI
160 170 180 190 200
APFADILTPN IPECFKLLGE DREISKLRDI FEVAKDLAKI TKCSNILVKG
210 220 230 240 250
GHIPWNDEEG KYITDVLYLG AEQRFITFKG NFVNTTHTHG TGCTLASAIA
260 270 280 290 300
SNLARGYSLP QSVYGGIEYV QNAVAIGCDV TKETVKDNGP INHVYAIEIP
310 320 330 340 350
LEKMLSDECF TASDAVHKKP VKSSLNKIPG GSFYKYLINH PKVKPHWDSY
360 370 380 390 400
VNHDFVRKVA DGSLEPKKFQ FFIEQDYLYL VNYARISCIA GSKSPCLEDL
410 420 430 440 450
EKELVIVECV RNGLCQHERR LREEFGIKDP DYLQKIQRGP ALRAYCRYFN
460 470 480 490 500
DVSRRGNWQE LVIALNPCLM GYVHALTKIK DEVTAAEGSV YREWCETYSS
510 520 530 540 550
SWCHEAMLEG EKLLNHILET YPPEKLDTLV TIYAEVCELE ANFWTAALEY

E
Length:551
Mass (Da):61,334
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB8FD8D7DF6A25961
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Z73614 Genomic DNA Translation: CAA97986.1
BK006949 Genomic DNA Translation: DAA11177.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S65289

NCBI Reference Sequences

More...
RefSeqi
NP_015065.1, NM_001184072.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YPL258C_mRNA; YPL258C; YPL258C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
855870

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YPL258C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73614 Genomic DNA Translation: CAA97986.1
BK006949 Genomic DNA Translation: DAA11177.1
PIRiS65289
RefSeqiNP_015065.1, NM_001184072.1

3D structure databases

SMRiQ08975
ModBaseiSearch...

Protein-protein interaction databases

BioGridi35954, 52 interactors
DIPiDIP-5635N
IntActiQ08975, 41 interactors
MINTiQ08975
STRINGi4932.YPL258C

Proteomic databases

PaxDbiQ08975
PRIDEiQ08975

Genome annotation databases

EnsemblFungiiYPL258C_mRNA; YPL258C; YPL258C
GeneIDi855870
KEGGisce:YPL258C

Organism-specific databases

EuPathDBiFungiDB:YPL258C
SGDiS000006179 THI21

Phylogenomic databases

HOGENOMiHOG000225275
InParanoidiQ08975
KOiK00877
OMAiAHSVYGM

Enzyme and pathway databases

UniPathwayiUPA00060;UER00137
UPA00060;UER00138
BioCyciMetaCyc:MONOMER3O-81
YEAST:MONOMER3O-81

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q08975
RNActiQ08975 protein

Family and domain databases

CDDicd01169 HMPP_kinase, 1 hit
Gene3Di1.20.910.10, 1 hit
3.40.1190.20, 1 hit
InterProiView protein in InterPro
IPR016084 Haem_Oase-like_multi-hlx
IPR004399 HMP/HMP-P_kinase
IPR013749 PM/HMP-P_kinase-1
IPR029056 Ribokinase-like
IPR004305 Thiaminase-2/PQQC
IPR027574 Thiaminase_II
PfamiView protein in Pfam
PF08543 Phos_pyr_kin, 1 hit
PF03070 TENA_THI-4, 1 hit
SUPFAMiSSF48613 SSF48613, 1 hit
SSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00097 HMP-P_kinase, 1 hit
TIGR04306 salvage_TenA, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTHI21_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q08975
Secondary accession number(s): D6W3B1
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: December 11, 2019
This is version 142 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
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