UniProtKB - Q08963 (RU2A_YEAST)
U2 small nuclear ribonucleoprotein A'
LEA1
Functioni
Involved in pre-mRNA splicing. Associates to U2 snRNA in a MSL1 dependent manner and is required for normal accumulation of U2 snRNA. Required for the spliceosome assembly and the efficient addition of U2 snRNP onto the pre-mRNA.
1 PublicationMiscellaneous
GO - Molecular functioni
- U2 snRNA binding Source: GO_Central
GO - Biological processi
- mRNA splicing, via spliceosome Source: SGD
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Names & Taxonomyi
Protein namesi | Recommended name: U2 small nuclear ribonucleoprotein A'Short name: U2 snRNP A' Alternative name(s): Looks exceptionally like U2A protein 1 |
Gene namesi | Name:LEA1 Ordered Locus Names:YPL213W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006134, LEA1 |
VEuPathDBi | FungiDB:YPL213W |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- U2 snRNP Source: SGD
- U2-type prespliceosome Source: SGD
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074189 | 1 – 238 | U2 small nuclear ribonucleoprotein A'Add BLAST | 238 |
Proteomic databases
MaxQBi | Q08963 |
PaxDbi | Q08963 |
PRIDEi | Q08963 |
Interactioni
Subunit structurei
Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
Interacts with MSL1.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 35972, 693 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex CPX-26, U2 small nuclear ribonucleoprotein complex |
DIPi | DIP-2399N |
IntActi | Q08963, 30 interactors |
MINTi | Q08963 |
STRINGi | 4932.YPL213W |
Miscellaneous databases
RNActi | Q08963, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q08963 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 53 – 74 | LRR 1Add BLAST | 22 | |
Repeati | 75 – 95 | LRR 2Add BLAST | 21 | |
Repeati | 97 – 118 | LRR 3Add BLAST | 22 | |
Domaini | 132 – 170 | LRRCTAdd BLAST | 39 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 167 – 189 | DisorderedSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Keywords - Domaini
Leucine-rich repeat, RepeatPhylogenomic databases
eggNOGi | KOG1644, Eukaryota |
GeneTreei | ENSGT00940000153289 |
HOGENOMi | CLU_061027_3_0_1 |
OMAi | CHLEDYR |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf IPR044640, RU2A IPR003603, U2A'_phosphoprotein32A_C |
PANTHERi | PTHR10552, PTHR10552, 1 hit |
SMARTi | View protein in SMART SM00446, LRRcap, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 3 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL
60 70 80 90 100
TKPTHILDLT NNDLIMIPDL SRRDDIHTLL LGRNNIVEVD GRLLPMNVQN
110 120 130 140 150
LTLSNNSIRR FEDLQRLRRA PRTLKNLTLI GNQVCHLANY REHVLRLVPH
160 170 180 190 200
LETLDFQNVT AEERKSAMSF PRQADGDTLG PVNTAIRDNG SRDKTMEIMN
210 220 230
LVVSKMTVER RNELKKQLAE ATSLEEIARL EKLLSGGV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z73569 Genomic DNA Translation: CAA97928.1 AY558137 Genomic DNA Translation: AAS56463.1 BK006949 Genomic DNA Translation: DAA11223.1 |
PIRi | S65232 |
RefSeqi | NP_015111.1, NM_001184027.1 |
Genome annotation databases
EnsemblFungii | YPL213W_mRNA; YPL213W; YPL213W |
GeneIDi | 855888 |
KEGGi | sce:YPL213W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z73569 Genomic DNA Translation: CAA97928.1 AY558137 Genomic DNA Translation: AAS56463.1 BK006949 Genomic DNA Translation: DAA11223.1 |
PIRi | S65232 |
RefSeqi | NP_015111.1, NM_001184027.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5GMK | electron microscopy | 3.40 | b | 1-238 | [»] | |
5LJ3 | electron microscopy | 3.80 | W | 1-238 | [»] | |
5LJ5 | electron microscopy | 3.80 | W | 1-238 | [»] | |
5MQ0 | electron microscopy | 4.17 | W | 1-238 | [»] | |
5NRL | electron microscopy | 7.20 | W | 1-238 | [»] | |
5WSG | electron microscopy | 4.00 | Y | 1-238 | [»] | |
5Y88 | electron microscopy | 3.70 | o | 1-238 | [»] | |
5YLZ | electron microscopy | 3.60 | o | 1-238 | [»] | |
5ZWM | electron microscopy | 3.40 | o | 1-238 | [»] | |
5ZWO | electron microscopy | 3.90 | o | 1-238 | [»] | |
6BK8 | electron microscopy | 3.30 | s | 1-238 | [»] | |
6EXN | electron microscopy | 3.70 | W | 1-238 | [»] | |
6G90 | electron microscopy | 4.00 | W | 1-238 | [»] | |
6J6G | electron microscopy | 3.20 | b | 1-238 | [»] | |
6J6H | electron microscopy | 3.60 | b | 1-238 | [»] | |
6J6N | electron microscopy | 3.86 | b | 1-238 | [»] | |
6J6Q | electron microscopy | 3.70 | b | 1-238 | [»] | |
7OQB | electron microscopy | 9.00 | W | 1-238 | [»] | |
7OQE | electron microscopy | 5.90 | W | 1-238 | [»] | |
SMRi | Q08963 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35972, 693 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex CPX-26, U2 small nuclear ribonucleoprotein complex |
DIPi | DIP-2399N |
IntActi | Q08963, 30 interactors |
MINTi | Q08963 |
STRINGi | 4932.YPL213W |
Proteomic databases
MaxQBi | Q08963 |
PaxDbi | Q08963 |
PRIDEi | Q08963 |
Genome annotation databases
EnsemblFungii | YPL213W_mRNA; YPL213W; YPL213W |
GeneIDi | 855888 |
KEGGi | sce:YPL213W |
Organism-specific databases
SGDi | S000006134, LEA1 |
VEuPathDBi | FungiDB:YPL213W |
Phylogenomic databases
eggNOGi | KOG1644, Eukaryota |
GeneTreei | ENSGT00940000153289 |
HOGENOMi | CLU_061027_3_0_1 |
OMAi | CHLEDYR |
Miscellaneous databases
PROi | PR:Q08963 |
RNActi | Q08963, protein |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf IPR044640, RU2A IPR003603, U2A'_phosphoprotein32A_C |
PANTHERi | PTHR10552, PTHR10552, 1 hit |
SMARTi | View protein in SMART SM00446, LRRcap, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | RU2A_YEAST | |
Accessioni | Q08963Primary (citable) accession number: Q08963 Secondary accession number(s): D6W3F7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2005 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families