UniProtKB - Q08949 (DDC1_YEAST)
DNA damage checkpoint protein 1
DDC1
Functioni
Component of the checkpoint clamp complex involved in the surveillance mechanism that allows the DNA repair pathways to act to restore the integrity of the DNA prior to DNA synthesis or separation of the replicated chromosomes. Associates with sites of DNA damage and modulates the MEC1 signaling pathway and the activation of RAD53 in response to DNA damage at phase G1. The complex also physically regulates DNA polymerase zeta-dependent mutagenesis by controlling the access of polymerase zeta to damaged DNA.
10 PublicationsMiscellaneous
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- protein kinase activator activity Source: SGD
GO - Biological processi
- activation of protein kinase activity Source: SGD
- cellular response to ionizing radiation Source: GO_Central
- DNA damage checkpoint signaling Source: ComplexPortal
- DNA repair Source: GO_Central
- DNA replication checkpoint signaling Source: GO_Central
- meiotic cell cycle Source: SGD
- meiotic recombination checkpoint signaling Source: SGD
- mitotic G1 DNA damage checkpoint signaling Source: SGD
- mitotic G2 DNA damage checkpoint signaling Source: SGD
- mitotic intra-S DNA damage checkpoint signaling Source: SGD
- recombinational repair Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA repair |
Enzyme and pathway databases
Reactomei | R-SCE-176187, Activation of ATR in response to replication stress |
Names & Taxonomyi
Protein namesi | Recommended name: DNA damage checkpoint protein 1 |
Gene namesi | Name:DDC1 Ordered Locus Names:YPL194W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006115, DDC1 |
VEuPathDBi | FungiDB:YPL194W |
Subcellular locationi
Nucleus
- checkpoint clamp complex Source: SGD
- condensed nuclear chromosome Source: SGD
- nucleus Source: SGD
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000239638 | 1 – 612 | DNA damage checkpoint protein 1Add BLAST | 612 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 436 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q08949 |
PaxDbi | Q08949 |
PRIDEi | Q08949 |
PTM databases
iPTMneti | Q08949 |
Interactioni
Subunit structurei
Component of the checkpoint clamp complex composed of DDC1, MEC3 and RAD17. The interaction with MEC3 is performed in a RAD17-dependent manner. The checkpoint clamp complex loads onto DNA in an ATP-dependent manner through its interaction with the RFC-RAD4 checkpoint clamp loader complex.
Interacts with the DNA polymerase zeta subunit REV7 and DPB11.
9 PublicationsBinary interactionsi
Q08949
With | #Exp. | IntAct |
---|---|---|
MEC3 [Q02574] | 5 | EBI-30769,EBI-10658 |
RAD17 [P48581] | 4 | EBI-30769,EBI-14652 |
REV7 [P38927] | 3 | EBI-30769,EBI-14960 |
Protein-protein interaction databases
BioGRIDi | 35989, 223 interactors |
ComplexPortali | CPX-1806, Rad17-Mec3-Ddc1 checkpoint clamp complex |
DIPi | DIP-2323N |
IntActi | Q08949, 13 interactors |
MINTi | Q08949 |
STRINGi | 4932.YPL194W |
Miscellaneous databases
RNActi | Q08949, protein |
Structurei
3D structure databases
AlphaFoldDBi | Q08949 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 576 – 612 | DisorderedSequence analysisAdd BLAST | 37 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 586 – 601 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QT39, Eukaryota |
HOGENOMi | CLU_490204_0_0_1 |
InParanoidi | Q08949 |
OMAi | TTLCQVR |
Family and domain databases
InterProi | View protein in InterPro IPR026217, Ddc1 IPR007268, Rad9/Ddc1 |
PANTHERi | PTHR15237, PTHR15237, 1 hit |
Pfami | View protein in Pfam PF04139, Rad9, 2 hits |
PRINTSi | PR02063, DNADAMAGECP1 |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSFKATITES GKQNIWFRAI YVLSTIQDDI KITVTTNELI AWSMNETDTT
60 70 80 90 100
LCQVRFQKSF FEEYEFKPHE IVFGENGVQV IEDTYGNSHK LYSFRVNGRH
110 120 130 140 150
LTTISRKPDG DGIKSFTIAV NNTSTCPESL ANRLIVVIEM DSLIVKEYCP
160 170 180 190 200
QFQPIKYDPI IINLKYKRRF LDVFGTAASD RNPQEPLDPK LLDVFTNTER
210 220 230 240 250
ELTSALFNEE VESDIRKRNQ LTAADEINYI CCNSTLLKNF LDNCNVNVTD
260 270 280 290 300
EVKLEINVHR LSITAFTKAV YGKNNDLLRN ALSMSNTIST LDLEHYCLFT
310 320 330 340 350
TIEDEKQDKR SHSKRREHMK SIIFKLKDFK NFITIGPSWK TTQDGNDNIS
360 370 380 390 400
LWFCHPGDPI LMQMQKPGVK LELVEVTDSN INDDILEGKF IKTAISGSKE
410 420 430 440 450
EAGLKDNKES CESPLKSKTA LKRENLPHSV AGTRNSPLKV SYLTPDNGST
460 470 480 490 500
VAKTYRNNTA RKLFVEEQSQ STNYEQDKRF RQASSVHMNM NREQSFDIGT
510 520 530 540 550
THEVACPRNE SNSLKRSIAD ICNETEDPTQ QSTFAKRADT TVTWGKALPA
560 570 580 590 600
ADDEVSCSNI DRKGMLKKEK LKHMQGLLNS QNDTSNHKKQ DNKEMEDGLG
610
LTQVEKPRGI FD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z73550 Genomic DNA Translation: CAA97907.1 BK006949 Genomic DNA Translation: DAA11240.1 |
PIRi | S65213 |
RefSeqi | NP_015130.1, NM_001184008.1 |
Genome annotation databases
EnsemblFungii | YPL194W_mRNA; YPL194W; YPL194W |
GeneIDi | 855907 |
KEGGi | sce:YPL194W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z73550 Genomic DNA Translation: CAA97907.1 BK006949 Genomic DNA Translation: DAA11240.1 |
PIRi | S65213 |
RefSeqi | NP_015130.1, NM_001184008.1 |
3D structure databases
AlphaFoldDBi | Q08949 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 35989, 223 interactors |
ComplexPortali | CPX-1806, Rad17-Mec3-Ddc1 checkpoint clamp complex |
DIPi | DIP-2323N |
IntActi | Q08949, 13 interactors |
MINTi | Q08949 |
STRINGi | 4932.YPL194W |
PTM databases
iPTMneti | Q08949 |
Proteomic databases
MaxQBi | Q08949 |
PaxDbi | Q08949 |
PRIDEi | Q08949 |
Genome annotation databases
EnsemblFungii | YPL194W_mRNA; YPL194W; YPL194W |
GeneIDi | 855907 |
KEGGi | sce:YPL194W |
Organism-specific databases
SGDi | S000006115, DDC1 |
VEuPathDBi | FungiDB:YPL194W |
Phylogenomic databases
eggNOGi | ENOG502QT39, Eukaryota |
HOGENOMi | CLU_490204_0_0_1 |
InParanoidi | Q08949 |
OMAi | TTLCQVR |
Enzyme and pathway databases
Reactomei | R-SCE-176187, Activation of ATR in response to replication stress |
Miscellaneous databases
PROi | PR:Q08949 |
RNActi | Q08949, protein |
Family and domain databases
InterProi | View protein in InterPro IPR026217, Ddc1 IPR007268, Rad9/Ddc1 |
PANTHERi | PTHR15237, PTHR15237, 1 hit |
Pfami | View protein in Pfam PF04139, Rad9, 2 hits |
PRINTSi | PR02063, DNADAMAGECP1 |
MobiDBi | Search... |
Entry informationi
Entry namei | DDC1_YEAST | |
Accessioni | Q08949Primary (citable) accession number: Q08949 Secondary accession number(s): D6W3H4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 13, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - SIMILARITY comments
Index of protein domains and families