UniProtKB - Q08945 (SSRP1_HUMAN)
FACT complex subunit SSRP1
SSRP1
Functioni
Miscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 547 – 615 | HMG boxPROSITE-ProRule annotationAdd BLAST | 69 |
GO - Molecular functioni
- DNA binding Source: ProtInc
- histone binding Source: GO_Central
- nucleosome binding Source: GO_Central
- RNA binding Source: UniProtKB
GO - Biological processi
- DNA repair Source: UniProtKB-KW
- DNA replication Source: UniProtKB-KW
- regulation of chromatin organization Source: CACAO
- regulation of signal transduction by p53 class mediator Source: Reactome
- transcription by RNA polymerase II Source: Reactome
- transcription elongation from RNA polymerase II promoter Source: Reactome
- viral process Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA repair, DNA replication, Host-virus interaction, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q08945 |
Reactomei | R-HSA-112382, Formation of RNA Pol II elongation complex R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation R-HSA-167243, Tat-mediated HIV elongation arrest and recovery R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript R-HSA-167287, HIV elongation arrest and recovery R-HSA-167290, Pausing and recovery of HIV elongation R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes R-HSA-6804756, Regulation of TP53 Activity through Phosphorylation R-HSA-75955, RNA Polymerase II Transcription Elongation |
SIGNORi | Q08945 |
Names & Taxonomyi
Protein namesi | Recommended name: FACT complex subunit SSRP1Alternative name(s): Chromatin-specific transcription elongation factor 80 kDa subunit Facilitates chromatin transcription complex 80 kDa subunit Short name: FACT 80 kDa subunit Short name: FACTp80 Facilitates chromatin transcription complex subunit SSRP1 Recombination signal sequence recognition protein 1 Structure-specific recognition protein 1 Short name: hSSRP1 T160 |
Gene namesi | Name:SSRP1 Synonyms:FACT80 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11327, SSRP1 |
MIMi | 604328, gene |
neXtProti | NX_Q08945 |
VEuPathDBi | HostDB:ENSG00000149136.8 |
Subcellular locationi
Nucleus
Other locations
- Chromosome 1 Publication
Note: Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.By similarity
Nucleus
- FACT complex Source: GO_Central
- nucleolus Source: UniProtKB-SubCell
- nucleoplasm Source: Reactome
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 450 | D → A: Abolishes cleavage by caspase. 1 Publication | 1 | |
Mutagenesisi | 510 | S → A: Unable to bind DNA; when associated with A-657 and A-688. 1 Publication | 1 | |
Mutagenesisi | 657 | S → A: Unable to bind DNA; when associated with A-510 and A-688. Still able to bind DNA; when associated with A-688. 1 Publication | 1 | |
Mutagenesisi | 688 | S → A: Unable to bind DNA; when associated with A-510 and A-657. Still able to bind DNA; when associated with A-657. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6749 |
OpenTargetsi | ENSG00000149136 |
PharmGKBi | PA36151 |
Miscellaneous databases
Pharosi | Q08945, Tbio |
Genetic variation databases
BioMutai | SSRP1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000048606 | 2 – 709 | FACT complex subunit SSRP1Add BLAST | 708 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | |
Cross-linki | 90 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 170 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 233 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 296 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 364 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 413 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 437 | PhosphoserineCombined sources | 1 | |
Modified residuei | 441 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 444 | PhosphoserineCombined sources | 1 | |
Modified residuei | 452 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 471 | PhosphoserineCombined sources | 1 | |
Modified residuei | 510 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 542 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 657 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 659 | PhosphoserineCombined sources | 1 | |
Modified residuei | 667 | PhosphoserineCombined sources | 1 | |
Modified residuei | 668 | PhosphoserineCombined sources | 1 | |
Modified residuei | 671 | PhosphoserineCombined sources | 1 | |
Modified residuei | 672 | PhosphoserineCombined sources | 1 | |
Modified residuei | 673 | PhosphoserineCombined sources | 1 | |
Modified residuei | 688 | Phosphoserine; by CK21 Publication | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 450 – 451 | Cleavage; by caspase-3 and/or caspase-7 | 2 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q08945 |
jPOSTi | Q08945 |
MassIVEi | Q08945 |
MaxQBi | Q08945 |
PaxDbi | Q08945 |
PeptideAtlasi | Q08945 |
PRIDEi | Q08945 |
ProteomicsDBi | 58651 |
PTM databases
iPTMneti | Q08945 |
MetOSitei | Q08945 |
PhosphoSitePlusi | Q08945 |
SwissPalmi | Q08945 |
Expressioni
Gene expression databases
Bgeei | ENSG00000149136, Expressed in tendon of biceps brachii and 240 other tissues |
ExpressionAtlasi | Q08945, baseline and differential |
Genevisiblei | Q08945, HS |
Organism-specific databases
HPAi | ENSG00000149136, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with MYOG (via C-terminal region) (By similarity).
Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H (PubMed:10421373). Also component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B (PubMed:11239457, PubMed:12393879). Binds to histone H3-H4 tetramers, but not to intact nucleosomes.
Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1 (PubMed:27499292).
Interacts with isoform gamma of TP63 (PubMed:12374749).
Interacts with FYTTD1/UIF (PubMed:19836239).
Interacts with SRF (By similarity).
Interacts with NEK9 (PubMed:14660563).
By similarity7 Publications(Microbial infection) Interacts with Herpes simplex virus 1 (HHV-1) protein ICP22; this interaction relocalizes the FACT complex to viral genomes in infected cells.
1 PublicationBinary interactionsi
Hide detailsQ08945
GO - Molecular functioni
- histone binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 112627, 201 interactors |
ComplexPortali | CPX-419, FACT complex |
CORUMi | Q08945 |
DIPi | DIP-169N |
IntActi | Q08945, 91 interactors |
MINTi | Q08945 |
STRINGi | 9606.ENSP00000278412 |
Miscellaneous databases
RNActi | Q08945, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q08945 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 439 – 496 | Asp/Glu-rich (acidic)Add BLAST | 58 | |
Compositional biasi | 497 – 511 | Ser-richAdd BLAST | 15 | |
Compositional biasi | 512 – 534 | Arg/Lys-rich (basic)Add BLAST | 23 | |
Compositional biasi | 623 – 640 | Arg/Lys-rich (basic)Add BLAST | 18 | |
Compositional biasi | 641 – 709 | Ser-richAdd BLAST | 69 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0526, Eukaryota |
GeneTreei | ENSGT00940000157117 |
HOGENOMi | CLU_017374_2_1_1 |
InParanoidi | Q08945 |
OMAi | PPIHIRF |
OrthoDBi | 915055at2759 |
PhylomeDBi | Q08945 |
TreeFami | TF315228 |
Family and domain databases
Gene3Di | 1.10.30.10, 1 hit 2.30.29.220, 1 hit 2.30.29.30, 2 hits |
InterProi | View protein in InterPro IPR013719, DUF1747 IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf IPR011993, PH-like_dom_sf IPR035417, POB3_N IPR000969, SSrcognition IPR024954, SSRP1_dom IPR038167, SSRP1_sf |
Pfami | View protein in Pfam PF00505, HMG_box, 1 hit PF17292, POB3_N, 1 hit PF08512, Rtt106, 1 hit PF03531, SSrecog, 1 hit |
PRINTSi | PR00887, SSRCOGNITION |
SMARTi | View protein in SMART SM00398, HMG, 1 hit SM01287, Rtt106, 1 hit |
SUPFAMi | SSF47095, SSF47095, 1 hit |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAETLEFNDV YQEVKGSMND GRLRLSRQGI IFKNSKTGKV DNIQAGELTE
60 70 80 90 100
GIWRRVALGH GLKLLTKNGH VYKYDGFRES EFEKLSDFFK THYRLELMEK
110 120 130 140 150
DLCVKGWNWG TVKFGGQLLS FDIGDQPVFE IPLSNVSQCT TGKNEVTLEF
160 170 180 190 200
HQNDDAEVSL MEVRFYVPPT QEDGVDPVEA FAQNVLSKAD VIQATGDAIC
210 220 230 240 250
IFRELQCLTP RGRYDIRIYP TFLHLHGKTF DYKIPYTTVL RLFLLPHKDQ
260 270 280 290 300
RQMFFVISLD PPIKQGQTRY HFLILLFSKD EDISLTLNMN EEEVEKRFEG
310 320 330 340 350
RLTKNMSGSL YEMVSRVMKA LVNRKITVPG NFQGHSGAQC ITCSYKASSG
360 370 380 390 400
LLYPLERGFI YVHKPPVHIR FDEISFVNFA RGTTTTRSFD FEIETKQGTQ
410 420 430 440 450
YTFSSIEREE YGKLFDFVNA KKLNIKNRGL KEGMNPSYDE YADSDEDQHD
460 470 480 490 500
AYLERMKEEG KIREENANDS SDDSGEETDE SFNPGEEEED VAEEFDSNAS
510 520 530 540 550
ASSSSNEGDS DRDEKKRKQL KKAKMAKDRK SRKKPVEVKK GKDPNAPKRP
560 570 580 590 600
MSAYMLWLNA SREKIKSDHP GISITDLSKK AGEIWKGMSK EKKEEWDRKA
610 620 630 640 650
EDARRDYEKA MKEYEGGRGE SSKRDKSKKK KKVKVKMEKK STPSRGSSSK
660 670 680 690 700
SSSRQLSESF KSKEFVSSDE SSSGENKSKK KRRRSEDSEE EELASTPPSS
EDSASGSDE
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PMD4 | E9PMD4_HUMAN | FACT complex subunit SSRP1 | SSRP1 | 173 | Annotation score: | ||
A0A0U1RRK2 | A0A0U1RRK2_HUMAN | FACT complex subunit SSRP1 | SSRP1 | 225 | Annotation score: | ||
E9PPZ7 | E9PPZ7_HUMAN | FACT complex subunit SSRP1 | SSRP1 | 89 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052495 | 225 | L → V. Corresponds to variant dbSNP:rs768436Ensembl. | 1 | |
Natural variantiVAR_052496 | 458 | E → Q. Corresponds to variant dbSNP:rs11540304Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86737 mRNA Translation: AAA58660.1 BC005116 mRNA Translation: AAH05116.1 BC091486 mRNA Translation: AAH91486.1 Sequence problems. |
CCDSi | CCDS7952.1 |
PIRi | A41976 |
RefSeqi | NP_003137.1, NM_003146.2 |
Genome annotation databases
Ensembli | ENST00000278412; ENSP00000278412; ENSG00000149136 |
GeneIDi | 6749 |
KEGGi | hsa:6749 |
UCSCi | uc001njt.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86737 mRNA Translation: AAA58660.1 BC005116 mRNA Translation: AAH05116.1 BC091486 mRNA Translation: AAH91486.1 Sequence problems. |
CCDSi | CCDS7952.1 |
PIRi | A41976 |
RefSeqi | NP_003137.1, NM_003146.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4IFS | X-ray | 1.93 | A | 196-430 | [»] | |
5UMR | X-ray | 1.50 | A | 1-100 | [»] | |
5UMS | X-ray | 1.57 | A | 174-437 | [»] | |
5VWE | NMR | - | A | 551-617 | [»] | |
6L1E | X-ray | 2.09 | A | 196-430 | [»] | |
6L1R | X-ray | 1.80 | A | 1-100 | [»] | |
6L34 | X-ray | 2.00 | A | 548-615 | [»] | |
6UPK | electron microscopy | 4.90 | H | 1-640 | [»] | |
6UPL | electron microscopy | 7.40 | H | 1-640 | [»] | |
SMRi | Q08945 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112627, 201 interactors |
ComplexPortali | CPX-419, FACT complex |
CORUMi | Q08945 |
DIPi | DIP-169N |
IntActi | Q08945, 91 interactors |
MINTi | Q08945 |
STRINGi | 9606.ENSP00000278412 |
PTM databases
iPTMneti | Q08945 |
MetOSitei | Q08945 |
PhosphoSitePlusi | Q08945 |
SwissPalmi | Q08945 |
Genetic variation databases
BioMutai | SSRP1 |
Proteomic databases
EPDi | Q08945 |
jPOSTi | Q08945 |
MassIVEi | Q08945 |
MaxQBi | Q08945 |
PaxDbi | Q08945 |
PeptideAtlasi | Q08945 |
PRIDEi | Q08945 |
ProteomicsDBi | 58651 |
Protocols and materials databases
Antibodypediai | 1060, 344 antibodies |
DNASUi | 6749 |
Genome annotation databases
Ensembli | ENST00000278412; ENSP00000278412; ENSG00000149136 |
GeneIDi | 6749 |
KEGGi | hsa:6749 |
UCSCi | uc001njt.3, human |
Organism-specific databases
CTDi | 6749 |
DisGeNETi | 6749 |
GeneCardsi | SSRP1 |
HGNCi | HGNC:11327, SSRP1 |
HPAi | ENSG00000149136, Low tissue specificity |
MIMi | 604328, gene |
neXtProti | NX_Q08945 |
OpenTargetsi | ENSG00000149136 |
PharmGKBi | PA36151 |
VEuPathDBi | HostDB:ENSG00000149136.8 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0526, Eukaryota |
GeneTreei | ENSGT00940000157117 |
HOGENOMi | CLU_017374_2_1_1 |
InParanoidi | Q08945 |
OMAi | PPIHIRF |
OrthoDBi | 915055at2759 |
PhylomeDBi | Q08945 |
TreeFami | TF315228 |
Enzyme and pathway databases
PathwayCommonsi | Q08945 |
Reactomei | R-HSA-112382, Formation of RNA Pol II elongation complex R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation R-HSA-167243, Tat-mediated HIV elongation arrest and recovery R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript R-HSA-167287, HIV elongation arrest and recovery R-HSA-167290, Pausing and recovery of HIV elongation R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes R-HSA-6804756, Regulation of TP53 Activity through Phosphorylation R-HSA-75955, RNA Polymerase II Transcription Elongation |
SIGNORi | Q08945 |
Miscellaneous databases
BioGRID-ORCSi | 6749, 761 hits in 1030 CRISPR screens |
ChiTaRSi | SSRP1, human |
GeneWikii | Structure_specific_recognition_protein_1 |
GenomeRNAii | 6749 |
Pharosi | Q08945, Tbio |
PROi | PR:Q08945 |
RNActi | Q08945, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000149136, Expressed in tendon of biceps brachii and 240 other tissues |
ExpressionAtlasi | Q08945, baseline and differential |
Genevisiblei | Q08945, HS |
Family and domain databases
Gene3Di | 1.10.30.10, 1 hit 2.30.29.220, 1 hit 2.30.29.30, 2 hits |
InterProi | View protein in InterPro IPR013719, DUF1747 IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf IPR011993, PH-like_dom_sf IPR035417, POB3_N IPR000969, SSrcognition IPR024954, SSRP1_dom IPR038167, SSRP1_sf |
Pfami | View protein in Pfam PF00505, HMG_box, 1 hit PF17292, POB3_N, 1 hit PF08512, Rtt106, 1 hit PF03531, SSrecog, 1 hit |
PRINTSi | PR00887, SSRCOGNITION |
SMARTi | View protein in SMART SM00398, HMG, 1 hit SM01287, Rtt106, 1 hit |
SUPFAMi | SSF47095, SSF47095, 1 hit |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SSRP1_HUMAN | |
Accessioni | Q08945Primary (citable) accession number: Q08945 Secondary accession number(s): Q5BJG8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 215 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families