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Protein

Nuclear cap-binding protein subunit 2

Gene

CBC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the CBC complex, which binds co-transcriptionally to the cap of pre-mRNAs and is involved in maturation, export and degradation of nuclear mRNAs. The CBC complex is required for efficient pre-mRNA splicing through efficient commitment complex and spliceosome formation. Together with NPL3, the CBC complex is required for export of mRNAs out of the nucleus. The CBC complex is also involved in nuclear mRNA degradation, probably by directing the mRNAs to the sites of degradation. Affects replication of the positive-strand RNA virus BMV.10 Publications

Miscellaneous

In contrast to metazoans, where the CBC complex is involved in the nuclear export of capped U snRNAs, it is believed that in yeast, U snRNAs are not exported from the nucleus and U snRNPs are assembled in the nucleus from RNAs and imported proteins.
Present with 6200 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei24mRNA capBy similarity1
Binding sitei49mRNA capBy similarity1

GO - Molecular functioni

  • RNA cap binding Source: SGD

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34073-MONOMER
ReactomeiR-SCE-113418 Formation of the Early Elongation Complex
R-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-72086 mRNA Capping
R-SCE-72165 mRNA Splicing - Minor Pathway
R-SCE-77595 Processing of Intronless Pre-mRNAs
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear cap-binding protein subunit 2
Alternative name(s):
20 kDa nuclear cap-binding protein
NCBP 20 kDa subunit
Short name:
CBP20
Gene namesi
Name:CBC2
Synonyms:CBP20, MUD13, SAE1
Ordered Locus Names:YPL178W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL178W
SGDiS000006099 CBC2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002329901 – 208Nuclear cap-binding protein subunit 2Add BLAST208

Proteomic databases

MaxQBiQ08920
PaxDbiQ08920
PRIDEiQ08920

Interactioni

Subunit structurei

Component of the nuclear cap-binding complex (CBC), a heterodimer composed of STO1/CBC1 and CBC2 that interacts with capped RNAs. The complex interacts strongly with the importin subunit alpha SRP1. The SRP1-CBC trimer also binds to capped RNAs, but formation of the importin alpha/beta heterodimer upon binding of KAP95 to SRP1 in the cytoplasm causes dissociation of CBC from the RNA. The CBC complex is part of the commitment complex 1 (CC1), binding to the cap of pre-mRNA and interacting with U1 snRNP subunits MUD2 and SNU56. The CBC complex is part of the NRD1 complex, composed of CBC2, NAB1, NRD1, SEN1 and STO1/CBC2. The CBC complex also interacts with NPL3 and eIF4G (TIF4631 and TIF4632).7 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi36005, 671 interactors
ComplexPortaliCPX-1657 Nuclear cap-binding complex
DIPiDIP-1117N
IntActiQ08920, 27 interactors
MINTiQ08920
STRINGi4932.YPL178W

Structurei

3D structure databases

ProteinModelPortaliQ08920
SMRiQ08920
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 124RRMPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 122mRNA cap-bindingBy similarity5
Regioni129 – 133mRNA cap-bindingBy similarity5
Regioni139 – 140mRNA cap-bindingBy similarity2

Sequence similaritiesi

Belongs to the RRM NCBP2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003197
HOGENOMiHOG000217589
InParanoidiQ08920
KOiK12883
OMAiRFKFTPC
OrthoDBiEOG092C4JSY

Family and domain databases

CDDicd12240 RRM_NCBP2, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR027157 NCBP2
IPR034148 NCBP2_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR18847 PTHR18847, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequencei

Sequence statusi: Complete.

Q08920-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLEEFDEVK YDHSTKRLDT PSRYLLRKAR RNPNGLQELR ESMKSSTIYV
60 70 80 90 100
GNLSFYTSEE QIYELFSKCG TIKRIIMGLD RFKFTPCGFC FIIYSCPDEA
110 120 130 140 150
LNALKYLSDT KLDEKTITID LDPGFEDGRQ FGRGKSGGQV SDELRFDFDA
160 170 180 190 200
SRGGFAIPFA ERVGVPHSRF DNSSSQSNTN NYIPPPDAMG TFRPGFDEER

EDDNYVPQ
Length:208
Mass (Da):23,774
Last modified:November 1, 1996 - v1
Checksum:iB77E93BDDC429160
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39665 mRNA Translation: AAF21454.1
Z73534 Genomic DNA Translation: CAA97885.1
AY692949 Genomic DNA Translation: AAT92968.1
BK006949 Genomic DNA Translation: DAA11256.1
PIRiS65190
RefSeqiNP_015147.1, NM_001183992.1

Genome annotation databases

EnsemblFungiiYPL178W; YPL178W; YPL178W
GeneIDi855925
KEGGisce:YPL178W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39665 mRNA Translation: AAF21454.1
Z73534 Genomic DNA Translation: CAA97885.1
AY692949 Genomic DNA Translation: AAT92968.1
BK006949 Genomic DNA Translation: DAA11256.1
PIRiS65190
RefSeqiNP_015147.1, NM_001183992.1

3D structure databases

ProteinModelPortaliQ08920
SMRiQ08920
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36005, 671 interactors
ComplexPortaliCPX-1657 Nuclear cap-binding complex
DIPiDIP-1117N
IntActiQ08920, 27 interactors
MINTiQ08920
STRINGi4932.YPL178W

Proteomic databases

MaxQBiQ08920
PaxDbiQ08920
PRIDEiQ08920

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL178W; YPL178W; YPL178W
GeneIDi855925
KEGGisce:YPL178W

Organism-specific databases

EuPathDBiFungiDB:YPL178W
SGDiS000006099 CBC2

Phylogenomic databases

GeneTreeiENSGT00390000003197
HOGENOMiHOG000217589
InParanoidiQ08920
KOiK12883
OMAiRFKFTPC
OrthoDBiEOG092C4JSY

Enzyme and pathway databases

BioCyciYEAST:G3O-34073-MONOMER
ReactomeiR-SCE-113418 Formation of the Early Elongation Complex
R-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-72086 mRNA Capping
R-SCE-72165 mRNA Splicing - Minor Pathway
R-SCE-77595 Processing of Intronless Pre-mRNAs
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Miscellaneous databases

PROiPR:Q08920

Family and domain databases

CDDicd12240 RRM_NCBP2, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR027157 NCBP2
IPR034148 NCBP2_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR18847 PTHR18847, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNCBP2_YEAST
AccessioniPrimary (citable) accession number: Q08920
Secondary accession number(s): D6W3J0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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