Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable glutathione-independent glyoxalase HSP33

Gene

HSP33

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of methylglyoxal (MG) to D-lactate in a single glutathione (GSH)-independent step. May play a role in detoxifying endogenously produced glyoxals. Involved in protection against reactive oxygen species (ROS) (By similarity). Important for viability in stationary phase. May negatively regulate TORC1 in response to nutrient limitation (PubMed:24706893).By similarity1 Publication

Catalytic activityi

(R)-lactate = methylglyoxal + H2O.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1381 Publication1
Active sitei1391 Publication1
Active sitei1701 Publication1

GO - Molecular functioni

  • cysteine-type peptidase activity Source: SGD
  • lyase activity Source: UniProtKB-KW
  • unfolded protein binding Source: SGD

GO - Biological processi

  • cellular response to nutrient levels Source: SGD

Keywordsi

Molecular functionLyase
Biological processStress response

Enzyme and pathway databases

BioCyciYEAST:G3O-33852-MONOMER

Protein family/group databases

MEROPSiC56.A03

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutathione-independent glyoxalase HSP33By similarity (EC:4.2.1.130By similarity)
Alternative name(s):
Glyoxalase 3 homolog 3By similarity
Heat shock protein 331 Publication
Gene namesi
Name:HSP331 Publication
Ordered Locus Names:YOR391CImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR391C
SGDiS000005918 HSP33

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Results in higher sensitivity to oxidative stress, reduced thermotolerance, accumulation of higher levels of reactive oxygen species, and reduced chronological life span.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002705561 – 237Probable glutathione-independent glyoxalase HSP33Add BLAST237

Proteomic databases

MaxQBiQ08914
PaxDbiQ08914
PRIDEiQ08914

Expressioni

Inductioni

Induced during entry into stationary phase.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi34772, 27 interactors
IntActiQ08914, 3 interactors
MINTiQ08914
STRINGi4932.YOR391C

Structurei

Secondary structure

1237
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ08914
SMRiQ08914
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08914

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000013431
HOGENOMiHOG000181653
InParanoidiQ08914
KOiK22211
OMAiCHGPVIL
OrthoDBiEOG092C4O4J

Family and domain databases

Gene3Di3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
SUPFAMiSSF52317 SSF52317, 1 hit

Sequencei

Sequence statusi: Complete.

Q08914-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTPKRALISL TSYHGPFYKD GAKTGVFVVE ILRSFDTFEK HGFEVDFVSE
60 70 80 90 100
TGGFGWDEHY LPKSFIGGED KMNFETKNSA FNKALARIKT ANEVNASDYK
110 120 130 140 150
VFFASAGHGA LFDYPKAKNL QDIASKIYAN GGVIAAICHG PLLFDGLIDI
160 170 180 190 200
KTTRPLIEGK AITGFPLEGE IALGVDDILR SRKLTTVERV ANKNGAKYLA
210 220 230
PIHPWDDYSI TDGKLVTGVN ANSSYSTTIR AINALYS
Length:237
Mass (Da):25,926
Last modified:November 1, 1996 - v1
Checksum:i0E817A44022231A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75299 Genomic DNA Translation: CAA99723.1
BK006948 Genomic DNA Translation: DAA11150.1
RefSeqiNP_015036.3, NM_001183811.3

Genome annotation databases

EnsemblFungiiYOR391C; YOR391C; YOR391C
GeneIDi854573
KEGGisce:YOR391C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75299 Genomic DNA Translation: CAA99723.1
BK006948 Genomic DNA Translation: DAA11150.1
RefSeqiNP_015036.3, NM_001183811.3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KKLX-ray2.03A/B1-237[»]
3MIIX-ray2.40A/B1-237[»]
ProteinModelPortaliQ08914
SMRiQ08914
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34772, 27 interactors
IntActiQ08914, 3 interactors
MINTiQ08914
STRINGi4932.YOR391C

Protein family/group databases

MEROPSiC56.A03

Proteomic databases

MaxQBiQ08914
PaxDbiQ08914
PRIDEiQ08914

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR391C; YOR391C; YOR391C
GeneIDi854573
KEGGisce:YOR391C

Organism-specific databases

EuPathDBiFungiDB:YOR391C
SGDiS000005918 HSP33

Phylogenomic databases

GeneTreeiENSGT00390000013431
HOGENOMiHOG000181653
InParanoidiQ08914
KOiK22211
OMAiCHGPVIL
OrthoDBiEOG092C4O4J

Enzyme and pathway databases

BioCyciYEAST:G3O-33852-MONOMER

Miscellaneous databases

EvolutionaryTraceiQ08914
PROiPR:Q08914

Family and domain databases

Gene3Di3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
SUPFAMiSSF52317 SSF52317, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHSP33_YEAST
AccessioniPrimary (citable) accession number: Q08914
Secondary accession number(s): D6W384
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again