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Protein

Surfactin synthase subunit 3

Gene

srfAC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably activates a leucine.

Caution

The phosphoserine observed at Ser-1003 in PubMed:17218307 may result from the secondary neutral loss of pantetheine from the phosphodiester linked cofactor.Curated

Cofactori

pantetheine 4'-phosphateNote: Binds 1 phosphopantetheine covalently.

Pathwayi: surfactin biosynthesis

This protein is involved in the pathway surfactin biosynthesis, which is part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the pathway surfactin biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11201
Active sitei11471
Active sitei12471

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAntibiotic biosynthesis, Sporulation

Enzyme and pathway databases

BioCyciBSUB:BSU03510-MONOMER
SABIO-RKiQ08787
UniPathwayiUPA00181

Protein family/group databases

ESTHERibacsu-srfac Thioesterase

Names & Taxonomyi

Protein namesi
Recommended name:
Surfactin synthase subunit 3
Gene namesi
Name:srfAC
Synonyms:srfA3
Ordered Locus Names:BSU03510
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931011 – 1275Surfactin synthase subunit 3Add BLAST1275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1003O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1 Publication1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

PaxDbiQ08787
PRIDEiQ08787

Interactioni

Protein-protein interaction databases

IntActiQ08787, 2 interactors
MINTiQ08787
STRINGi224308.Bsubs1_010100001993

Structurei

Secondary structure

11275
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Beta strandi9 – 14Combined sources6
Helixi17 – 25Combined sources9
Helixi291 – 293Combined sources3
Beta strandi300 – 307Combined sources8
Helixi314 – 328Combined sources15
Helixi329 – 331Combined sources3
Helixi336 – 341Combined sources6
Beta strandi342 – 344Combined sources3
Beta strandi346 – 348Combined sources3
Beta strandi352 – 355Combined sources4
Helixi360 – 363Combined sources4
Helixi368 – 371Combined sources4
Beta strandi372 – 380Combined sources9
Beta strandi386 – 393Combined sources8
Beta strandi395 – 397Combined sources3
Beta strandi399 – 405Combined sources7
Turni406 – 408Combined sources3
Helixi411 – 430Combined sources20
Helixi436 – 438Combined sources3
Helixi444 – 451Combined sources8
Turni452 – 454Combined sources3
Helixi466 – 476Combined sources11
Beta strandi480 – 487Combined sources8
Beta strandi489 – 491Combined sources3
Helixi492 – 508Combined sources17
Beta strandi516 – 519Combined sources4
Helixi525 – 536Combined sources12
Beta strandi540 – 543Combined sources4
Helixi550 – 560Combined sources11
Beta strandi564 – 567Combined sources4
Beta strandi582 – 588Combined sources7
Helixi590 – 594Combined sources5
Beta strandi608 – 615Combined sources8
Beta strandi618 – 621Combined sources4
Beta strandi623 – 628Combined sources6
Helixi629 – 636Combined sources8
Beta strandi649 – 652Combined sources4
Helixi660 – 669Combined sources10
Turni670 – 672Combined sources3
Beta strandi674 – 677Combined sources4
Helixi680 – 682Combined sources3
Helixi686 – 696Combined sources11
Beta strandi700 – 704Combined sources5
Helixi705 – 714Combined sources10
Helixi717 – 720Combined sources4
Beta strandi723 – 730Combined sources8
Helixi734 – 744Combined sources11
Beta strandi749 – 753Combined sources5
Helixi756 – 758Combined sources3
Beta strandi762 – 766Combined sources5
Beta strandi780 – 782Combined sources3
Beta strandi786 – 791Combined sources6
Beta strandi804 – 810Combined sources7
Helixi821 – 827Combined sources7
Beta strandi828 – 830Combined sources3
Beta strandi838 – 848Combined sources11
Beta strandi854 – 859Combined sources6
Helixi860 – 862Combined sources3
Beta strandi863 – 866Combined sources4
Beta strandi869 – 872Combined sources4
Helixi873 – 882Combined sources10
Beta strandi883 – 885Combined sources3
Beta strandi888 – 894Combined sources7
Beta strandi897 – 899Combined sources3
Beta strandi902 – 908Combined sources7
Beta strandi910 – 913Combined sources4
Helixi915 – 925Combined sources11
Beta strandi933 – 936Combined sources4
Beta strandi944 – 947Combined sources4
Helixi951 – 954Combined sources4
Beta strandi970 – 973Combined sources4
Beta strandi983 – 985Combined sources3
Helixi1001 – 1014Combined sources14
Beta strandi1030 – 1034Combined sources5
Helixi1037 – 1040Combined sources4
Beta strandi1044 – 1046Combined sources3
Beta strandi1050 – 1054Combined sources5
Beta strandi1058 – 1064Combined sources7
Helixi1071 – 1074Combined sources4
Helixi1075 – 1080Combined sources6
Beta strandi1084 – 1089Combined sources6
Helixi1097 – 1108Combined sources12
Beta strandi1114 – 1119Combined sources6
Helixi1121 – 1135Combined sources15
Beta strandi1140 – 1147Combined sources8
Beta strandi1156 – 1163Combined sources8
Helixi1166 – 1172Combined sources7
Turni1173 – 1175Combined sources3
Helixi1178 – 1180Combined sources3
Helixi1182 – 1201Combined sources20
Beta strandi1208 – 1216Combined sources9
Beta strandi1225 – 1228Combined sources4
Helixi1231 – 1233Combined sources3
Beta strandi1234 – 1236Combined sources3
Beta strandi1238 – 1242Combined sources5
Helixi1247 – 1249Combined sources3
Helixi1253 – 1267Combined sources15

3D structure databases

ProteinModelPortaliQ08787
SMRiQ08787
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08787

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini968 – 1043CarrierPROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1059 – 1271ThioesteraseAdd BLAST213

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3319 LUCA
HOGENOMiHOG000229993
InParanoidiQ08787
KOiK15656
OMAiFSVLARY
PhylomeDBiQ08787

Family and domain databases

Gene3Di1.10.1200.10, 1 hit
3.30.559.10, 2 hits
3.40.50.1820, 2 hits
InterProiView protein in InterPro
IPR010071 AA_adenyl_domain
IPR029058 AB_hydrolase
IPR036736 ACP-like_sf
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR023213 CAT-like_dom_sf
IPR001242 Condensatn
IPR020806 PKS_PP-bd
IPR009081 PP-bd_ACP
IPR006162 Ppantetheine_attach_site
IPR001031 Thioesterase
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PF00668 Condensation, 1 hit
PF00550 PP-binding, 1 hit
PF00975 Thioesterase, 1 hit
SMARTiView protein in SMART
SM00823 PKS_PP, 1 hit
SUPFAMiSSF47336 SSF47336, 1 hit
SSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR01733 AA-adenyl-dom, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
PS50075 CARRIER, 1 hit
PS00012 PHOSPHOPANTETHEINE, 1 hit

Sequencei

Sequence statusi: Complete.

Q08787-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQFSKDQVQ DMYYLSPMQE GMLFHAILNP GQSFYLEQIT MKVKGSLNIK
60 70 80 90 100
CLEESMNVIM DRYDVFRTVF IHEKVKRPVQ VVLKKRQFHI EEIDLTHLTG
110 120 130 140 150
SEQTAKINEY KEQDKIRGFD LTRDIPMRAA IFKKAEESFE WVWSYHHIIL
160 170 180 190 200
DGWCFGIVVQ DLFKVYNALR EQKPYSLPPV KPYKDYIKWL EKQDKQASLR
210 220 230 240 250
YWREYLEGFE GQTTFAEQRK KQKDGYEPKE LLFSLSEAET KAFTELAKSQ
260 270 280 290 300
HTTLSTALQA VWSVLISRYQ QSGDLAFGTV VSGRPAEIKG VEHMVGLFIN
310 320 330 340 350
VVPRRVKLSE GITFNGLLKR LQEQSLQSEP HQYVPLYDIQ SQADQPKLID
360 370 380 390 400
HIIVFENYPL QDAKNEESSE NGFDMVDVHV FEKSNYDLNL MASPGDEMLI
410 420 430 440 450
KLAYNENVFD EAFILRLKSQ LLTAIQQLIQ NPDQPVSTIN LVDDREREFL
460 470 480 490 500
LTGLNPPAQA HETKPLTYWF KEAVNANPDA PALTYSGQTL SYRELDEEAN
510 520 530 540 550
RIARRLQKHG AGKGSVVALY TKRSLELVIG ILGVLKAGAA YLPVDPKLPE
560 570 580 590 600
DRISYMLADS AAACLLTHQE MKEQAAELPY TGTTLFIDDQ TRFEEQASDP
610 620 630 640 650
ATAIDPNDPA YIMYTSGTTG KPKGNITTHA NIQGLVKHVD YMAFSDQDTF
660 670 680 690 700
LSVSNYAFDA FTFDFYASML NAARLIIADE HTLLDTERLT DLILQENVNV
710 720 730 740 750
MFATTALFNL LTDAGEDWMK GLRCILFGGE RASVPHVRKA LRIMGPGKLI
760 770 780 790 800
NCYGPTEGTV FATAHVVHDL PDSISSLPIG KPISNASVYI LNEQSQLQPF
810 820 830 840 850
GAVGELCISG MGVSKGYVNR ADLTKEKFIE NPFKPGETLY RTGDLARWLP
860 870 880 890 900
DGTIEYAGRI DDQVKIRGHR IELEEIEKQL QEYPGVKDAV VVADRHESGD
910 920 930 940 950
ASINAYLVNR TQLSAEDVKA HLKKQLPAYM VPQTFTFLDE LPLTTNGKVN
960 970 980 990 1000
KRLLPKPDQD QLAEEWIGPR NEMEETIAQI WSEVLGRKQI GIHDDFFALG
1010 1020 1030 1040 1050
GHSLKAMTAA SRIKKELGID LPVKLLFEAP TIAGISAYLK NGGSDGLQDV
1060 1070 1080 1090 1100
TIMNQDQEQI IFAFPPVLGY GLMYQNLSSR LPSYKLCAFD FIEEEDRLDR
1110 1120 1130 1140 1150
YADLIQKLQP EGPLTLFGYS AGCSLAFEAA KKLEEQGRIV QRIIMVDSYK
1160 1170 1180 1190 1200
KQGVSDLDGR TVESDVEALM NVNRDNEALN SEAVKHGLKQ KTHAFYSYYV
1210 1220 1230 1240 1250
NLISTGQVKA DIDLLTSGAD FDMPEWLASW EEATTGVYRV KRGFGTHAEM
1260 1270
LQGETLDRNA EILLEFLNTQ TVTVS
Length:1,275
Mass (Da):143,872
Last modified:June 16, 2009 - v2
Checksum:iC17C1685127BAF56
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26A → P in CAA49818 (PubMed:8355609).Curated1
Sequence conflicti26A → P in BAA08985 (PubMed:8969502).Curated1
Sequence conflicti33S → T in CAA49818 (PubMed:8355609).Curated1
Sequence conflicti33S → T in BAA08985 (PubMed:8969502).Curated1
Sequence conflicti1010 – 1015ASRIKK → VPHQQ in CAA49818 (PubMed:8355609).Curated6
Sequence conflicti1010 – 1015ASRIKK → VPHQQ in BAA08985 (PubMed:8969502).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70356 Genomic DNA Translation: CAA49818.1
D50453 Genomic DNA Translation: BAA08985.1
AL009126 Genomic DNA Translation: CAB12145.2
PIRiI40487
RefSeqiNP_388233.2, NC_000964.3
WP_003234570.1, NZ_JNCM01000031.1

Genome annotation databases

EnsemblBacteriaiCAB12145; CAB12145; BSU03510
GeneIDi938308
KEGGibsu:BSU03510
PATRICifig|224308.179.peg.368

Similar proteinsi

Entry informationi

Entry nameiSRFAC_BACSU
AccessioniPrimary (citable) accession number: Q08787
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 16, 2009
Last modified: July 18, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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