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Protein

Surfactin synthase subunit 3

Gene

srfAC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably activates a leucine.

Caution

The phosphoserine observed at Ser-1003 in PubMed:17218307 may result from the secondary neutral loss of pantetheine from the phosphodiester linked cofactor.Curated

Cofactori

pantetheine 4'-phosphateNote: Binds 1 phosphopantetheine covalently.

Pathwayi: surfactin biosynthesis

This protein is involved in the pathway surfactin biosynthesis, which is part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the pathway surfactin biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11201
Active sitei11471
Active sitei12471

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAntibiotic biosynthesis, Sporulation

Enzyme and pathway databases

BioCyciBSUB:BSU03510-MONOMER
SABIO-RKiQ08787
UniPathwayi
UPA00181

Protein family/group databases

ESTHERibacsu-srfac Thioesterase

Names & Taxonomyi

Protein namesi
Recommended name:
Surfactin synthase subunit 3
Gene namesi
Name:srfAC
Synonyms:srfA3
Ordered Locus Names:BSU03510
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931011 – 1275Surfactin synthase subunit 3Add BLAST1275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1003O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1 Publication1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

PaxDbiQ08787
PRIDEiQ08787

Interactioni

Protein-protein interaction databases

IntActiQ08787, 2 interactors
MINTiQ08787
STRINGi224308.Bsubs1_010100001993

Structurei

Secondary structure

11275
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ08787
SMRiQ08787
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08787

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini968 – 1043CarrierPROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1059 – 1271ThioesteraseAdd BLAST213

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3319 LUCA
HOGENOMiHOG000229993
InParanoidiQ08787
KOiK15656
OMAiFSVLARY
PhylomeDBiQ08787

Family and domain databases

Gene3Di1.10.1200.10, 1 hit
3.30.559.10, 2 hits
3.40.50.1820, 2 hits
InterProiView protein in InterPro
IPR010071 AA_adenyl_domain
IPR029058 AB_hydrolase
IPR036736 ACP-like_sf
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR023213 CAT-like_dom_sf
IPR001242 Condensatn
IPR020806 PKS_PP-bd
IPR009081 PP-bd_ACP
IPR006162 Ppantetheine_attach_site
IPR001031 Thioesterase
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PF00668 Condensation, 1 hit
PF00550 PP-binding, 1 hit
PF00975 Thioesterase, 1 hit
SMARTiView protein in SMART
SM00823 PKS_PP, 1 hit
SUPFAMiSSF47336 SSF47336, 1 hit
SSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR01733 AA-adenyl-dom, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
PS50075 CARRIER, 1 hit
PS00012 PHOSPHOPANTETHEINE, 1 hit

Sequencei

Sequence statusi: Complete.

Q08787-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSQFSKDQVQ DMYYLSPMQE GMLFHAILNP GQSFYLEQIT MKVKGSLNIK
60 70 80 90 100
CLEESMNVIM DRYDVFRTVF IHEKVKRPVQ VVLKKRQFHI EEIDLTHLTG
110 120 130 140 150
SEQTAKINEY KEQDKIRGFD LTRDIPMRAA IFKKAEESFE WVWSYHHIIL
160 170 180 190 200
DGWCFGIVVQ DLFKVYNALR EQKPYSLPPV KPYKDYIKWL EKQDKQASLR
210 220 230 240 250
YWREYLEGFE GQTTFAEQRK KQKDGYEPKE LLFSLSEAET KAFTELAKSQ
260 270 280 290 300
HTTLSTALQA VWSVLISRYQ QSGDLAFGTV VSGRPAEIKG VEHMVGLFIN
310 320 330 340 350
VVPRRVKLSE GITFNGLLKR LQEQSLQSEP HQYVPLYDIQ SQADQPKLID
360 370 380 390 400
HIIVFENYPL QDAKNEESSE NGFDMVDVHV FEKSNYDLNL MASPGDEMLI
410 420 430 440 450
KLAYNENVFD EAFILRLKSQ LLTAIQQLIQ NPDQPVSTIN LVDDREREFL
460 470 480 490 500
LTGLNPPAQA HETKPLTYWF KEAVNANPDA PALTYSGQTL SYRELDEEAN
510 520 530 540 550
RIARRLQKHG AGKGSVVALY TKRSLELVIG ILGVLKAGAA YLPVDPKLPE
560 570 580 590 600
DRISYMLADS AAACLLTHQE MKEQAAELPY TGTTLFIDDQ TRFEEQASDP
610 620 630 640 650
ATAIDPNDPA YIMYTSGTTG KPKGNITTHA NIQGLVKHVD YMAFSDQDTF
660 670 680 690 700
LSVSNYAFDA FTFDFYASML NAARLIIADE HTLLDTERLT DLILQENVNV
710 720 730 740 750
MFATTALFNL LTDAGEDWMK GLRCILFGGE RASVPHVRKA LRIMGPGKLI
760 770 780 790 800
NCYGPTEGTV FATAHVVHDL PDSISSLPIG KPISNASVYI LNEQSQLQPF
810 820 830 840 850
GAVGELCISG MGVSKGYVNR ADLTKEKFIE NPFKPGETLY RTGDLARWLP
860 870 880 890 900
DGTIEYAGRI DDQVKIRGHR IELEEIEKQL QEYPGVKDAV VVADRHESGD
910 920 930 940 950
ASINAYLVNR TQLSAEDVKA HLKKQLPAYM VPQTFTFLDE LPLTTNGKVN
960 970 980 990 1000
KRLLPKPDQD QLAEEWIGPR NEMEETIAQI WSEVLGRKQI GIHDDFFALG
1010 1020 1030 1040 1050
GHSLKAMTAA SRIKKELGID LPVKLLFEAP TIAGISAYLK NGGSDGLQDV
1060 1070 1080 1090 1100
TIMNQDQEQI IFAFPPVLGY GLMYQNLSSR LPSYKLCAFD FIEEEDRLDR
1110 1120 1130 1140 1150
YADLIQKLQP EGPLTLFGYS AGCSLAFEAA KKLEEQGRIV QRIIMVDSYK
1160 1170 1180 1190 1200
KQGVSDLDGR TVESDVEALM NVNRDNEALN SEAVKHGLKQ KTHAFYSYYV
1210 1220 1230 1240 1250
NLISTGQVKA DIDLLTSGAD FDMPEWLASW EEATTGVYRV KRGFGTHAEM
1260 1270
LQGETLDRNA EILLEFLNTQ TVTVS
Length:1,275
Mass (Da):143,872
Last modified:June 16, 2009 - v2
Checksum:iC17C1685127BAF56
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26A → P in CAA49818 (PubMed:8355609).Curated1
Sequence conflicti26A → P in BAA08985 (PubMed:8969502).Curated1
Sequence conflicti33S → T in CAA49818 (PubMed:8355609).Curated1
Sequence conflicti33S → T in BAA08985 (PubMed:8969502).Curated1
Sequence conflicti1010 – 1015ASRIKK → VPHQQ in CAA49818 (PubMed:8355609).Curated6
Sequence conflicti1010 – 1015ASRIKK → VPHQQ in BAA08985 (PubMed:8969502).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70356 Genomic DNA Translation: CAA49818.1
D50453 Genomic DNA Translation: BAA08985.1
AL009126 Genomic DNA Translation: CAB12145.2
PIRiI40487
RefSeqiNP_388233.2, NC_000964.3
WP_003234570.1, NZ_JNCM01000031.1

Genome annotation databases

EnsemblBacteriaiCAB12145; CAB12145; BSU03510
GeneIDi938308
KEGGibsu:BSU03510
PATRICifig|224308.179.peg.368

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70356 Genomic DNA Translation: CAA49818.1
D50453 Genomic DNA Translation: BAA08985.1
AL009126 Genomic DNA Translation: CAB12145.2
PIRiI40487
RefSeqiNP_388233.2, NC_000964.3
WP_003234570.1, NZ_JNCM01000031.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JMKX-ray1.71C/O1042-1271[»]
2VSQX-ray2.60A1-1275[»]
ProteinModelPortaliQ08787
SMRiQ08787
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ08787, 2 interactors
MINTiQ08787
STRINGi224308.Bsubs1_010100001993

Protein family/group databases

ESTHERibacsu-srfac Thioesterase

Proteomic databases

PaxDbiQ08787
PRIDEiQ08787

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12145; CAB12145; BSU03510
GeneIDi938308
KEGGibsu:BSU03510
PATRICifig|224308.179.peg.368

Phylogenomic databases

eggNOGiCOG3319 LUCA
HOGENOMiHOG000229993
InParanoidiQ08787
KOiK15656
OMAiFSVLARY
PhylomeDBiQ08787

Enzyme and pathway databases

UniPathwayi
UPA00181

BioCyciBSUB:BSU03510-MONOMER
SABIO-RKiQ08787

Miscellaneous databases

EvolutionaryTraceiQ08787

Family and domain databases

Gene3Di1.10.1200.10, 1 hit
3.30.559.10, 2 hits
3.40.50.1820, 2 hits
InterProiView protein in InterPro
IPR010071 AA_adenyl_domain
IPR029058 AB_hydrolase
IPR036736 ACP-like_sf
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR023213 CAT-like_dom_sf
IPR001242 Condensatn
IPR020806 PKS_PP-bd
IPR009081 PP-bd_ACP
IPR006162 Ppantetheine_attach_site
IPR001031 Thioesterase
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PF00668 Condensation, 1 hit
PF00550 PP-binding, 1 hit
PF00975 Thioesterase, 1 hit
SMARTiView protein in SMART
SM00823 PKS_PP, 1 hit
SUPFAMiSSF47336 SSF47336, 1 hit
SSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR01733 AA-adenyl-dom, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
PS50075 CARRIER, 1 hit
PS00012 PHOSPHOPANTETHEINE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSRFAC_BACSU
AccessioniPrimary (citable) accession number: Q08787
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 16, 2009
Last modified: September 12, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
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Main funding by: National Institutes of Health

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