UniProtKB - Q08773 (ISW2_YEAST)
ISWI chromatin-remodeling complex ATPase ISW2
ISW2
Functioni
Catalytic component of the ISW2 complex, which acts in remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. The ISW2 complex is involved in coordinating transcriptional repression and in inheritance of telomeric silencing. It is involved in repression of MAT a-specific genes, INO1, and early meiotic genes during mitotic growth dependent upon transcription factor UME6 and in a parallel pathway to the RPD3-SIN3 histone deacetylase complex.
7 PublicationsMiscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 209 – 216 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP-dependent activity, acting on DNA Source: GO_Central
- ATP-dependent chromatin remodeler activity Source: InterPro
- centromeric DNA binding Source: SGD
- chromatin binding Source: SGD
- DNA binding Source: GO_Central
- DNA translocase activity Source: SGD
- helicase activity Source: UniProtKB-KW
- hydrolase activity Source: UniProtKB-KW
- nucleosome binding Source: InterPro
- single-stranded DNA binding Source: SGD
GO - Biological processi
- chromatin remodeling Source: SGD
- negative regulation of antisense RNA transcription Source: SGD
- negative regulation of transcription from RNA polymerase II promoter by pheromones Source: SGD
- nucleosome positioning Source: SGD
- positive regulation of transcription by RNA polymerase II Source: GO_Central
- rDNA heterochromatin assembly Source: SGD
- regulation of transcription, DNA-templated Source: ComplexPortal
- subtelomeric heterochromatin assembly Source: SGD
- termination of RNA polymerase II transcription Source: SGD
Keywordsi
Molecular function | Chromatin regulator, DNA-binding, Helicase, Hydrolase, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: ISWI chromatin-remodeling complex ATPase ISW2 (EC:3.6.4.-)Alternative name(s): Imitation switch protein 2 |
Gene namesi | Name:ISW2 Ordered Locus Names:YOR304W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005831, ISW2 |
VEuPathDBi | FungiDB:YOR304W |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Cytoskeleton
- nuclear microtubule Source: SGD
Nucleus
- CHRAC Source: SGD
- nuclear microtubule Source: SGD
- nucleus Source: SGD
Other locations
- chromosome, telomeric region Source: GOC
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 215 | K → A: Abolishes ATPase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000240453 | 1 – 1120 | ISWI chromatin-remodeling complex ATPase ISW2Add BLAST | 1120 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 17 | PhosphoserineCombined sources | 1 | |
Modified residuei | 19 | PhosphoserineCombined sources | 1 | |
Modified residuei | 831 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1079 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 1082 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q08773 |
PaxDbi | Q08773 |
PRIDEi | Q08773 |
PTM databases
iPTMneti | Q08773 |
Interactioni
Subunit structurei
Component of the ISW2 complex, which at least consists of ISW2, ITC1, DLS1 and DPB4. May form a stable subcomplex with ITC1.
3 PublicationsBinary interactionsi
Q08773
With | #Exp. | IntAct |
---|---|---|
ITC1 [P53125] | 2 | EBI-31118,EBI-23967 |
Protein-protein interaction databases
BioGRIDi | 34692, 343 interactors |
ComplexPortali | CPX-728, ISW2 chromatin remodeling complex CPX-734, ISW2 chromatin remodeling complex variant 2 |
DIPi | DIP-6603N |
IntActi | Q08773, 40 interactors |
MINTi | Q08773 |
STRINGi | 4932.YOR304W |
Miscellaneous databases
RNActi | Q08773, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 196 – 361 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 166 | |
Domaini | 494 – 645 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 152 | |
Domaini | 886 – 938 | SANTPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 58 | DisorderedSequence analysisAdd BLAST | 58 | |
Regioni | 129 – 153 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 764 – 783 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 828 – 853 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 1062 – 1120 | DisorderedSequence analysisAdd BLAST | 59 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 312 – 315 | DEAH box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 17 – 41 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 43 – 58 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 837 – 853 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 1076 – 1104 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 1105 – 1120 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0385, Eukaryota |
GeneTreei | ENSGT00940000176603 |
HOGENOMi | CLU_000315_0_0_1 |
InParanoidi | Q08773 |
OMAi | IHDWQFF |
Family and domain databases
CDDi | cd17997, DEXHc_SMARCA1_SMARCA5, 1 hit cd00167, SANT, 1 hit |
Gene3Di | 1.10.1040.30, 1 hit 3.40.50.10810, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR009057, Homeobox-like_sf IPR044754, Isw1/2_DEXHc IPR015194, ISWI_HAND-dom IPR036306, ISWI_HAND-dom_sf IPR027417, P-loop_NTPase IPR001005, SANT/Myb IPR017884, SANT_dom IPR015195, SLIDE IPR038718, SNF2-like_sf IPR000330, SNF2_N |
Pfami | View protein in Pfam PF09110, HAND, 1 hit PF00271, Helicase_C, 1 hit PF09111, SLIDE, 1 hit PF00176, SNF2-rel_dom, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00717, SANT, 2 hits |
SUPFAMi | SSF101224, SSF101224, 1 hit SSF46689, SSF46689, 2 hits SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51293, SANT, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTTQQEEQRS DTKNSKSESP SEVLVDTLDS KSNGSSDDDN IGQSEELSDK
60 70 80 90 100
EIYTVEDRPP EYWAQRKKKF VLDVDPKYAK QKDKSDTYKR FKYLLGVTDL
110 120 130 140 150
FRHFIGIKAK HDKNIQKLLK QLDSDANKLS KSHSTVSSSS RHHRKTEKEE
160 170 180 190 200
DAELMADEEE EIVDTYQEDI FVSESPSFVK SGKLRDYQVQ GLNWLISLHE
210 220 230 240 250
NKLSGILADE MGLGKTLQTI SFLGYLRYVK QIEGPFLIIV PKSTLDNWRR
260 270 280 290 300
EFLKWTPNVN VLVLHGDKDT RADIVRNIIL EARFDVLITS YEMVIREKNA
310 320 330 340 350
LKRLAWQYIV IDEAHRIKNE QSALSQIIRL FYSKNRLLIT GTPLQNNLHE
360 370 380 390 400
LWALLNFLLP DIFGDSELFD EWFEQNNSEQ DQEIVIQQLH SVLNPFLLRR
410 420 430 440 450
VKADVEKSLL PKIETNVYVG MTDMQIQWYK SLLEKDIDAV NGAVGKREGK
460 470 480 490 500
TRLLNIVMQL RKCCNHPYLF EGAEPGPPYT TDEHLIFNSG KMIILDKLLK
510 520 530 540 550
RLKEKGSRVL IFSQMSRLLD ILEDYCYFRD FEYCRIDGST SHEERIEAID
560 570 580 590 600
EYNKPNSEKF VFLLTTRAGG LGINLVTADT VILFDSDWNP QADLQAMDRA
610 620 630 640 650
HRIGQKKQVH VYRFVTENAI EEKVIERAAQ KLRLDQLVIQ QGTGKKTASL
660 670 680 690 700
GNSKDDLLDM IQFGAKNMFE KKASKVTVDA DIDDILKKGE QKTQELNAKY
710 720 730 740 750
QSLGLDDLQK FNGIENQSAY EWNGKSFQKK SNDKVVEWIN PSRRERRREQ
760 770 780 790 800
TTYSVDDYYK EIIGGGSKSA SKQTPQPKAP RAPKVIHGQD FQFFPKELDA
810 820 830 840 850
LQEKEQLYFK KKVNYKVTSY DITGDIRNEG SDAEEEEGEY KNAANTEGHK
860 870 880 890 900
GHEELKRRIE EEQEKINSAP DFTQEDELRK QELISKAFTN WNKRDFMAFI
910 920 930 940 950
NACAKYGRDD MENIKKSIDS KTPEEVEVYA KIFWERLKEI NGWEKYLHNV
960 970 980 990 1000
ELGEKKNEKL KFQETLLRQK IEQCKHPLHE LIIQYPPNNA RRTYNTLEDK
1010 1020 1030 1040 1050
FLLLAVNKYG LRADKLYEKL KQEIMMSDLF TFDWFIKTRT VHELSKRVHT
1060 1070 1080 1090 1100
LLTLIVREYE QPDANKKKRS RTSATREDTP LSQNESTRAS TVPNLPTTMV
1110 1120
TNQKDTNDHV DKRTKIDQEA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75212 Genomic DNA Translation: CAA99622.1 BK006948 Genomic DNA Translation: DAA11069.1 |
PIRi | S67208 |
RefSeqi | NP_014948.1, NM_001183724.1 |
Genome annotation databases
EnsemblFungii | YOR304W_mRNA; YOR304W; YOR304W |
GeneIDi | 854480 |
KEGGi | sce:YOR304W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75212 Genomic DNA Translation: CAA99622.1 BK006948 Genomic DNA Translation: DAA11069.1 |
PIRi | S67208 |
RefSeqi | NP_014948.1, NM_001183724.1 |
3D structure databases
AlphaFoldDBi | Q08773 |
SMRi | Q08773 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34692, 343 interactors |
ComplexPortali | CPX-728, ISW2 chromatin remodeling complex CPX-734, ISW2 chromatin remodeling complex variant 2 |
DIPi | DIP-6603N |
IntActi | Q08773, 40 interactors |
MINTi | Q08773 |
STRINGi | 4932.YOR304W |
PTM databases
iPTMneti | Q08773 |
Proteomic databases
MaxQBi | Q08773 |
PaxDbi | Q08773 |
PRIDEi | Q08773 |
Genome annotation databases
EnsemblFungii | YOR304W_mRNA; YOR304W; YOR304W |
GeneIDi | 854480 |
KEGGi | sce:YOR304W |
Organism-specific databases
SGDi | S000005831, ISW2 |
VEuPathDBi | FungiDB:YOR304W |
Phylogenomic databases
eggNOGi | KOG0385, Eukaryota |
GeneTreei | ENSGT00940000176603 |
HOGENOMi | CLU_000315_0_0_1 |
InParanoidi | Q08773 |
OMAi | IHDWQFF |
Miscellaneous databases
PROi | PR:Q08773 |
RNActi | Q08773, protein |
Family and domain databases
CDDi | cd17997, DEXHc_SMARCA1_SMARCA5, 1 hit cd00167, SANT, 1 hit |
Gene3Di | 1.10.1040.30, 1 hit 3.40.50.10810, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR009057, Homeobox-like_sf IPR044754, Isw1/2_DEXHc IPR015194, ISWI_HAND-dom IPR036306, ISWI_HAND-dom_sf IPR027417, P-loop_NTPase IPR001005, SANT/Myb IPR017884, SANT_dom IPR015195, SLIDE IPR038718, SNF2-like_sf IPR000330, SNF2_N |
Pfami | View protein in Pfam PF09110, HAND, 1 hit PF00271, Helicase_C, 1 hit PF09111, SLIDE, 1 hit PF00176, SNF2-rel_dom, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00717, SANT, 2 hits |
SUPFAMi | SSF101224, SSF101224, 1 hit SSF46689, SSF46689, 2 hits SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51293, SANT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ISW2_YEAST | |
Accessioni | Q08773Primary (citable) accession number: Q08773 Secondary accession number(s): D6W303 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - SIMILARITY comments
Index of protein domains and families