UniProtKB - Q08647 (PUS7_YEAST)
Protein
Multisubstrate pseudouridine synthase 7
Gene
PUS7
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes pseudouridylation at position 35 in U2 snRNA stem-loop II region which induces particular conformation of the mRNA-U2 snRNA duplex and places the nucleophile in an accessible position for the first step of splicing (PubMed:12426583, PubMed:12682021, PubMed:15611063, PubMed:18435545, PubMed:19114708, PubMed:25219674). Also catalyzes pseudouridylation at position 56 in U2 snRNA (PubMed:21131909). Catalyzes also pseudouridylation at position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr) (PubMed:14561887, PubMed:18332121, PubMed:25219674). Pseudouridine residues in tRNAs may stabilize the local RNA conformation, favor interactions with protein partners and play an important role in the stabilization of the codon-anticodon interaction with mRNA (PubMed:14561887). Also catalyzes pseudouridylation of mRNAs in response to heat shock: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAR-3' (PubMed:25219674).9 Publications
Miscellaneous
Present with 4150 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:5.4.99.272 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 256 | Nucleophile1 Publication | 1 |
GO - Molecular functioni
- pseudouridine synthase activity Source: SGD
- RNA binding Source: InterPro
- tRNA pseudouridine synthase activity Source: UniProtKB-EC
GO - Biological processi
- enzyme-directed rRNA pseudouridine synthesis Source: SGD
- mRNA processing Source: UniProtKB-KW
- mRNA pseudouridine synthesis Source: SGD
- pseudouridine synthesis Source: GO_Central
- RNA splicing Source: UniProtKB-KW
- snRNA pseudouridine synthesis Source: UniProtKB
- tRNA modification Source: Reactome
- tRNA pseudouridine synthesis Source: SGD
Keywordsi
Molecular function | Isomerase |
Biological process | mRNA processing, mRNA splicing, Ribosome biogenesis, rRNA processing, tRNA processing |
Enzyme and pathway databases
BRENDAi | 5.4.99.27, 984 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PUS7 Ordered Locus Names:YOR243C ORF Names:O5254 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YOR243C |
SGDi | S000005769, PUS7 |
Subcellular locationi
Nucleus
- box H/ACA snoRNP complex Source: UniProtKB
- nucleoplasm Source: Reactome
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Decreased level of pseudouridylated mRNAs.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 256 | D → A: Abolishes enzymatic activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000152561 | 2 – 676 | Multisubstrate pseudouridine synthase 7Add BLAST | 675 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | Q08647 |
PaxDbi | Q08647 |
PRIDEi | Q08647 |
TopDownProteomicsi | Q08647 |
PTM databases
iPTMneti | Q08647 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34634, 155 interactors |
DIPi | DIP-6762N |
IntActi | Q08647, 11 interactors |
MINTi | Q08647 |
STRINGi | 4932.YOR243C |
Miscellaneous databases
RNActi | Q08647, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 338 – 582 | TRUDPROSITE-ProRule annotationAdd BLAST | 245 |
Sequence similaritiesi
Belongs to the pseudouridine synthase TruD family.Curated
Phylogenomic databases
eggNOGi | KOG2339, Eukaryota |
GeneTreei | ENSGT00530000063554 |
HOGENOMi | CLU_005281_0_2_1 |
InParanoidi | Q08647 |
OMAi | MALREVT |
Family and domain databases
Gene3Di | 3.30.2350.20, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR001656, PsdUridine_synth_TruD IPR020119, PsdUridine_synth_TruD_CS IPR011760, PsdUridine_synth_TruD_insert IPR042214, TruD_catalytic |
PANTHERi | PTHR13326, PTHR13326, 1 hit |
Pfami | View protein in Pfam PF01142, TruD, 1 hit |
PIRSFi | PIRSF037016, Pseudouridin_synth_euk_prd, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00094, tRNA_TruD_broad, 1 hit |
PROSITEi | View protein in PROSITE PS50984, TRUD, 1 hit PS01268, UPF0024, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q08647-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDSSEATVK RPLDAHVGPS ENAAKKLKIE QRTQADGIHE ADVGITLFLS
60 70 80 90 100
PELPGFRGQI KQRYTDFLVN EIDQEGKVIH LTDKGFKMPK KPQRSKEEVN
110 120 130 140 150
AEKESEAARR QEFNVDPELR NQLVEIFGEE DVLKIESVYR TANKMETAKN
160 170 180 190 200
FEDKSVRTKI HQLLREAFKN ELESVTTDTN TFKIARSNRN SRTNKQEKIN
210 220 230 240 250
QTRDANGVEN WGYGPSKDFI HFTLHKENKD TMEAVNVITK LLRVPSRVIR
260 270 280 290 300
YAGTKDRRAV TCQRVSISKI GLDRLNALNR TLKGMIIGNY NFSDASLNLG
310 320 330 340 350
DLKGNEFVVV IRDVTTGNSE VSLEEIVSNG CKSLSENGFI NYFGMQRFGT
360 370 380 390 400
FSISTHTIGR ELLLSNWKKA AELILSDQDN VLPKSKEARK IWAETKDAAL
410 420 430 440 450
ALKQMPRQCL AENALLYSLS NQRKEEDGTY SENAYYTAIM KIPRNLRTMY
460 470 480 490 500
VHAYQSYVWN SIASKRIELH GLKLVVGDLV IDTSEKSPLI SGIDDEDFDE
510 520 530 540 550
DVREAQFIRA KAVTQEDIDS VKYTMEDVVL PSPGFDVLYP SNEELKQLYV
560 570 580 590 600
DILKADNMDP FNMRRKVRDF SLAGSYRTVI QKPKSLEYRI IHYDDPSQQL
610 620 630 640 650
VNTDLDILNN TRAKESGQKY MKAKLDRYMP DKGGEKTAVV LKFQLGTSAY
660 670
ATMALRELMK LETSRRGDMC DVKENI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75151 Genomic DNA Translation: CAA99464.1 BK006948 Genomic DNA Translation: DAA11011.1 |
PIRi | S67136 |
RefSeqi | NP_014886.1, NM_001183662.1 |
Genome annotation databases
EnsemblFungii | YOR243C_mRNA; YOR243C; YOR243C |
GeneIDi | 854417 |
KEGGi | sce:YOR243C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75151 Genomic DNA Translation: CAA99464.1 BK006948 Genomic DNA Translation: DAA11011.1 |
PIRi | S67136 |
RefSeqi | NP_014886.1, NM_001183662.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 34634, 155 interactors |
DIPi | DIP-6762N |
IntActi | Q08647, 11 interactors |
MINTi | Q08647 |
STRINGi | 4932.YOR243C |
PTM databases
iPTMneti | Q08647 |
Proteomic databases
MaxQBi | Q08647 |
PaxDbi | Q08647 |
PRIDEi | Q08647 |
TopDownProteomicsi | Q08647 |
Genome annotation databases
EnsemblFungii | YOR243C_mRNA; YOR243C; YOR243C |
GeneIDi | 854417 |
KEGGi | sce:YOR243C |
Organism-specific databases
EuPathDBi | FungiDB:YOR243C |
SGDi | S000005769, PUS7 |
Phylogenomic databases
eggNOGi | KOG2339, Eukaryota |
GeneTreei | ENSGT00530000063554 |
HOGENOMi | CLU_005281_0_2_1 |
InParanoidi | Q08647 |
OMAi | MALREVT |
Enzyme and pathway databases
BRENDAi | 5.4.99.27, 984 |
Miscellaneous databases
PROi | PR:Q08647 |
RNActi | Q08647, protein |
Family and domain databases
Gene3Di | 3.30.2350.20, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR001656, PsdUridine_synth_TruD IPR020119, PsdUridine_synth_TruD_CS IPR011760, PsdUridine_synth_TruD_insert IPR042214, TruD_catalytic |
PANTHERi | PTHR13326, PTHR13326, 1 hit |
Pfami | View protein in Pfam PF01142, TruD, 1 hit |
PIRSFi | PIRSF037016, Pseudouridin_synth_euk_prd, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00094, tRNA_TruD_broad, 1 hit |
PROSITEi | View protein in PROSITE PS50984, TRUD, 1 hit PS01268, UPF0024, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUS7_YEAST | |
Accessioni | Q08647Primary (citable) accession number: Q08647 Secondary accession number(s): D6W2U5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD