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Entry version 160 (18 Sep 2019)
Sequence version 1 (01 Nov 1996)
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Protein

Multisubstrate pseudouridine synthase 7

Gene

PUS7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes pseudouridylation at position 35 in U2 snRNA stem-loop II region which induces particular conformation of the mRNA-U2 snRNA duplex and places the nucleophile in an accessible position for the first step of splicing (PubMed:12426583, PubMed:12682021, PubMed:15611063, PubMed:18435545, PubMed:19114708, PubMed:25219674). Also catalyzes pseudouridylation at position 56 in U2 snRNA (PubMed:21131909). Catalyzes also pseudouridylation at position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr) (PubMed:14561887, PubMed:18332121, PubMed:25219674). Pseudouridine residues in tRNAs may stabilize the local RNA conformation, favor interactions with protein partners and play an important role in the stabilization of the codon-anticodon interaction with mRNA (PubMed:14561887). Also catalyzes pseudouridylation of mRNAs in response to heat shock: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAR-3' (PubMed:25219674).9 Publications

Miscellaneous

Present with 4150 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei256Nucleophile1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionIsomerase
Biological processmRNA processing, mRNA splicing, Ribosome biogenesis, rRNA processing, tRNA processing

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-33737-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
5.4.99.27 984

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Multisubstrate pseudouridine synthase 7 (EC:5.4.99.-2 Publications, EC:5.4.99.272 Publications)
Alternative name(s):
RNA pseudouridylate synthase 7
RNA-uridine isomerase 7
tRNA pseudouridine(13) synthase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:PUS7
Ordered Locus Names:YOR243C
ORF Names:O5254
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XV

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YOR243C

Saccharomyces Genome Database

More...
SGDi
S000005769 PUS7

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Decreased level of pseudouridylated mRNAs.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi256D → A: Abolishes enzymatic activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCombined sources
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001525612 – 676Multisubstrate pseudouridine synthase 7Add BLAST675

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q08647

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q08647

PRoteomics IDEntifications database

More...
PRIDEi
Q08647

Consortium for Top Down Proteomics

More...
TopDownProteomicsi
Q08647

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q08647

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
34634, 155 interactors

Database of interacting proteins

More...
DIPi
DIP-6762N

Protein interaction database and analysis system

More...
IntActi
Q08647, 11 interactors

Molecular INTeraction database

More...
MINTi
Q08647

STRING: functional protein association networks

More...
STRINGi
4932.YOR243C

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q08647

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini338 – 582TRUDPROSITE-ProRule annotationAdd BLAST245

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the pseudouridine synthase TruD family.Curated

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000203484

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q08647

KEGG Orthology (KO)

More...
KOi
K06176

Identification of Orthologs from Complete Genome Data

More...
OMAi
MALREVT

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.2350.20, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR001656 PsdUridine_synth_TruD
IPR020119 PsdUridine_synth_TruD_CS
IPR011760 PsdUridine_synth_TruD_insert
IPR042214 TruD_catalytic

The PANTHER Classification System

More...
PANTHERi
PTHR13326 PTHR13326, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01142 TruD, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF037016 Pseudouridin_synth_euk_prd, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55120 SSF55120, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00094 tRNA_TruD_broad, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50984 TRUD, 1 hit
PS01268 UPF0024, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

Q08647-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDSSEATVK RPLDAHVGPS ENAAKKLKIE QRTQADGIHE ADVGITLFLS
60 70 80 90 100
PELPGFRGQI KQRYTDFLVN EIDQEGKVIH LTDKGFKMPK KPQRSKEEVN
110 120 130 140 150
AEKESEAARR QEFNVDPELR NQLVEIFGEE DVLKIESVYR TANKMETAKN
160 170 180 190 200
FEDKSVRTKI HQLLREAFKN ELESVTTDTN TFKIARSNRN SRTNKQEKIN
210 220 230 240 250
QTRDANGVEN WGYGPSKDFI HFTLHKENKD TMEAVNVITK LLRVPSRVIR
260 270 280 290 300
YAGTKDRRAV TCQRVSISKI GLDRLNALNR TLKGMIIGNY NFSDASLNLG
310 320 330 340 350
DLKGNEFVVV IRDVTTGNSE VSLEEIVSNG CKSLSENGFI NYFGMQRFGT
360 370 380 390 400
FSISTHTIGR ELLLSNWKKA AELILSDQDN VLPKSKEARK IWAETKDAAL
410 420 430 440 450
ALKQMPRQCL AENALLYSLS NQRKEEDGTY SENAYYTAIM KIPRNLRTMY
460 470 480 490 500
VHAYQSYVWN SIASKRIELH GLKLVVGDLV IDTSEKSPLI SGIDDEDFDE
510 520 530 540 550
DVREAQFIRA KAVTQEDIDS VKYTMEDVVL PSPGFDVLYP SNEELKQLYV
560 570 580 590 600
DILKADNMDP FNMRRKVRDF SLAGSYRTVI QKPKSLEYRI IHYDDPSQQL
610 620 630 640 650
VNTDLDILNN TRAKESGQKY MKAKLDRYMP DKGGEKTAVV LKFQLGTSAY
660 670
ATMALRELMK LETSRRGDMC DVKENI
Length:676
Mass (Da):77,002
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4B63F137B781FCCB
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Z75151 Genomic DNA Translation: CAA99464.1
BK006948 Genomic DNA Translation: DAA11011.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S67136

NCBI Reference Sequences

More...
RefSeqi
NP_014886.1, NM_001183662.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YOR243C_mRNA; YOR243C; YOR243C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
854417

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YOR243C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75151 Genomic DNA Translation: CAA99464.1
BK006948 Genomic DNA Translation: DAA11011.1
PIRiS67136
RefSeqiNP_014886.1, NM_001183662.1

3D structure databases

SMRiQ08647
ModBaseiSearch...

Protein-protein interaction databases

BioGridi34634, 155 interactors
DIPiDIP-6762N
IntActiQ08647, 11 interactors
MINTiQ08647
STRINGi4932.YOR243C

PTM databases

iPTMnetiQ08647

Proteomic databases

MaxQBiQ08647
PaxDbiQ08647
PRIDEiQ08647
TopDownProteomicsiQ08647

Genome annotation databases

EnsemblFungiiYOR243C_mRNA; YOR243C; YOR243C
GeneIDi854417
KEGGisce:YOR243C

Organism-specific databases

EuPathDBiFungiDB:YOR243C
SGDiS000005769 PUS7

Phylogenomic databases

HOGENOMiHOG000203484
InParanoidiQ08647
KOiK06176
OMAiMALREVT

Enzyme and pathway databases

BioCyciYEAST:G3O-33737-MONOMER
BRENDAi5.4.99.27 984

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q08647

Family and domain databases

Gene3Di3.30.2350.20, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR001656 PsdUridine_synth_TruD
IPR020119 PsdUridine_synth_TruD_CS
IPR011760 PsdUridine_synth_TruD_insert
IPR042214 TruD_catalytic
PANTHERiPTHR13326 PTHR13326, 1 hit
PfamiView protein in Pfam
PF01142 TruD, 1 hit
PIRSFiPIRSF037016 Pseudouridin_synth_euk_prd, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit
TIGRFAMsiTIGR00094 tRNA_TruD_broad, 1 hit
PROSITEiView protein in PROSITE
PS50984 TRUD, 1 hit
PS01268 UPF0024, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPUS7_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q08647
Secondary accession number(s): D6W2U5
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 18, 2019
This is version 160 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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