UniProtKB - Q08602 (PGTA_RAT)
Geranylgeranyl transferase type-2 subunit alpha
Rabggta
Functioni
Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A.
6 PublicationsCatalytic activityi
- geranylgeranyl diphosphate + L-cysteinyl-[protein] = diphosphate + S-geranylgeranyl-L-cysteinyl-[protein]4 PublicationsEC:2.5.1.604 Publications
Activity regulationi
GO - Molecular functioni
- Rab geranylgeranyltransferase activity Source: UniProtKB
- small GTPase binding Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- protein geranylgeranylation Source: UniProtKB
Keywordsi
Molecular function | Prenyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-RNO-6803205, TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain R-RNO-8873719, RAB geranylgeranylation |
Names & Taxonomyi
Protein namesi | Recommended name: Geranylgeranyl transferase type-2 subunit alpha (EC:2.5.1.60)Alternative name(s): Geranylgeranyl transferase type II subunit alpha Rab geranyl-geranyltransferase subunit alpha Short name: Rab GG transferase alpha Short name: Rab GGTase alpha Rab geranylgeranyltransferase subunit alpha |
Gene namesi | Name:Rabggta Synonyms:Ggta |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621697, Rabggta |
Subcellular locationi
Other locations
- cytoplasm Source: GO_Central
- Rab-protein geranylgeranyltransferase complex Source: UniProtKB
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119759 | 1 – 567 | Geranylgeranyl transferase type-2 subunit alphaAdd BLAST | 567 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 98 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q08602 |
PaxDbi | Q08602 |
PRIDEi | Q08602 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000030483, Expressed in skeletal muscle tissue and 22 other tissues |
Genevisiblei | Q08602, RN |
Interactioni
Subunit structurei
Heterotrimer composed of RABGGTA, RABGGTB and CHM; within this trimer, RABGGTA and RABGGTB form the catalytic component B, while CHM (component A) mediates peptide substrate binding (PubMed:11675392, PubMed:10745007, PubMed:12620235, PubMed:18399557, PubMed:18756270, PubMed:19894725, PubMed:21520375, PubMed:22963166, PubMed:8505342). The Rab GGTase dimer (RGGT) interacts with CHM (component A) prior to Rab protein binding; the association is stabilized by geranylgeranyl pyrophosphate (GGpp) (PubMed:12620235). The CHM:RGGT:Rab complex is destabilized by GGpp (PubMed:12620235).
Interacts with non-phosphorylated form of RAB8A; phosphorylation of RAB8A at 'Thr-72' disrupts this interaction (By similarity).
By similarity9 PublicationsGO - Molecular functioni
- small GTPase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 248714, 3 interactors |
ComplexPortali | CPX-2249, Protein geranylgeranyltransferase type II complex |
DIPi | DIP-6137N |
IntActi | Q08602, 1 interactor |
STRINGi | 10116.ENSRNOP00000049261 |
Chemistry databases
BindingDBi | Q08602 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q08602 |
SMRi | Q08602 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q08602 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 44 – 78 | PFTA 1Add BLAST | 35 | |
Repeati | 88 – 122 | PFTA 2Add BLAST | 35 | |
Repeati | 124 – 158 | PFTA 3Add BLAST | 35 | |
Repeati | 159 – 193 | PFTA 4Add BLAST | 35 | |
Repeati | 207 – 241 | PFTA 5Add BLAST | 35 | |
Repeati | 363 – 397 | PFTA 6Add BLAST | 35 | |
Repeati | 442 – 463 | LRR 1Add BLAST | 22 | |
Repeati | 464 – 486 | LRR 2Add BLAST | 23 | |
Repeati | 487 – 508 | LRR 3Add BLAST | 22 | |
Repeati | 509 – 530 | LRR 4Add BLAST | 22 | |
Repeati | 534 – 555 | LRR 5Add BLAST | 22 |
Sequence similaritiesi
Keywords - Domaini
Leucine-rich repeat, RepeatPhylogenomic databases
eggNOGi | KOG0529, Eukaryota |
GeneTreei | ENSGT00550000075121 |
HOGENOMi | CLU_031996_3_2_1 |
InParanoidi | Q08602 |
OMAi | YNAWHHR |
OrthoDBi | 1527547at2759 |
PhylomeDBi | Q08602 |
TreeFami | TF315057 |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR032675, LRR_dom_sf IPR002088, Prenyl_trans_a IPR036254, RabGGT_asu_insert-dom_sf IPR009087, RabGGT_asu_insert-domain IPR032955, RabGGTase_alpha |
PANTHERi | PTHR11129:SF2, PTHR11129:SF2, 1 hit |
Pfami | View protein in Pfam PF01239, PPTA, 5 hits PF07711, RabGGT_insert, 1 hit |
SUPFAMi | SSF49594, SSF49594, 1 hit |
PROSITEi | View protein in PROSITE PS51147, PFTA, 6 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MHGRLKVKTS EEQAEAKRLE REQKLKLYQS ATQAVFQKRQ AGELDESVLE
60 70 80 90 100
LTSQILGANP DFATLWNCRR EVLQHLETEK SPEESAALVK AELGFLESCL
110 120 130 140 150
RVNPKSYGTW HHRCWLLSRL PEPNWARELE LCARFLEADE RNFHCWDYRR
160 170 180 190 200
FVAAQAAVAP AEELAFTDSL ITRNFSNYSS WHYRSCLLPQ LHPQPDSGPQ
210 220 230 240 250
GRLPENVLLK ELELVQNAFF TDPNDQSAWF YHRWLLGRAE PHDVLCCVHV
260 270 280 290 300
SREEACLSVC FSRPLTVGSR MGTLLLMVDE APLSVEWRTP DGRNRPSHVW
310 320 330 340 350
LCDLPAASLN DQLPQHTFRV IWTGSDSQKE CVLLKDRPEC WCRDSATDEQ
360 370 380 390 400
LFRCELSVEK STVLQSELES CKELQELEPE NKWCLLTIIL LMRALDPLLY
410 420 430 440 450
EKETLQYFST LKAVDPMRAA YLDDLRSKFL LENSVLKMEY ADVRVLHLAH
460 470 480 490 500
KDLTVLCHLE QLLLVTHLDL SHNRLRALPP ALAALRCLEV LQASDNALEN
510 520 530 540 550
VDGVANLPRL QELLLCNNRL QQSAAIQPLV SCPRLVLLNL QGNSLCQEEG
560
IQERLAEMLP SVSSILT
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A8I5ZK93 | A0A8I5ZK93_RAT | Geranylgeranyl transferase type-2 s... | Rabggta | 564 | Annotation score: | ||
A0A8I6GJ12 | A0A8I6GJ12_RAT | Geranylgeranyl transferase type-2 s... | Rabggta | 564 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 169 | S → D AA sequence (PubMed:8505342).Curated | 1 | |
Sequence conflicti | 227 | S → Q AA sequence (PubMed:8505342).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10415 mRNA Translation: AAA41998.1 S62096 mRNA Translation: AAB27018.1 BC086547 mRNA Translation: AAH86547.1 |
PIRi | A45977 |
RefSeqi | NP_113842.1, NM_031654.2 XP_006252071.1, XM_006252009.3 XP_006252072.1, XM_006252010.3 |
Genome annotation databases
GeneIDi | 58983 |
KEGGi | rno:58983 |
UCSCi | RGD:621697, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10415 mRNA Translation: AAA41998.1 S62096 mRNA Translation: AAB27018.1 BC086547 mRNA Translation: AAH86547.1 |
PIRi | A45977 |
RefSeqi | NP_113842.1, NM_031654.2 XP_006252071.1, XM_006252009.3 XP_006252072.1, XM_006252010.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DCE | X-ray | 2.00 | A/C | 1-567 | [»] | |
1LTX | X-ray | 2.70 | A | 1-567 | [»] | |
3C72 | X-ray | 2.30 | A | 1-236 | [»] | |
A | 353-441 | [»] | ||||
3DSS | X-ray | 1.80 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3DST | X-ray | 1.90 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3DSU | X-ray | 1.90 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3DSV | X-ray | 2.10 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3DSW | X-ray | 2.15 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3DSX | X-ray | 2.10 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3HXB | X-ray | 2.25 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3HXC | X-ray | 1.95 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3HXD | X-ray | 1.95 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3HXE | X-ray | 1.95 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3HXF | X-ray | 1.90 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3PZ1 | X-ray | 1.95 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3PZ2 | X-ray | 2.35 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
3PZ3 | X-ray | 2.00 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
4EHM | X-ray | 2.20 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
4GTS | X-ray | 2.45 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
4GTT | X-ray | 2.05 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
4GTV | X-ray | 1.95 | A | 1-237 | [»] | |
A | 353-441 | [»] | ||||
AlphaFoldDBi | Q08602 | |||||
SMRi | Q08602 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248714, 3 interactors |
ComplexPortali | CPX-2249, Protein geranylgeranyltransferase type II complex |
DIPi | DIP-6137N |
IntActi | Q08602, 1 interactor |
STRINGi | 10116.ENSRNOP00000049261 |
Chemistry databases
BindingDBi | Q08602 |
ChEMBLi | CHEMBL4523995 |
Proteomic databases
jPOSTi | Q08602 |
PaxDbi | Q08602 |
PRIDEi | Q08602 |
Genome annotation databases
GeneIDi | 58983 |
KEGGi | rno:58983 |
UCSCi | RGD:621697, rat |
Organism-specific databases
CTDi | 5875 |
RGDi | 621697, Rabggta |
Phylogenomic databases
eggNOGi | KOG0529, Eukaryota |
GeneTreei | ENSGT00550000075121 |
HOGENOMi | CLU_031996_3_2_1 |
InParanoidi | Q08602 |
OMAi | YNAWHHR |
OrthoDBi | 1527547at2759 |
PhylomeDBi | Q08602 |
TreeFami | TF315057 |
Enzyme and pathway databases
Reactomei | R-RNO-6803205, TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain R-RNO-8873719, RAB geranylgeranylation |
Miscellaneous databases
EvolutionaryTracei | Q08602 |
PROi | PR:Q08602 |
Gene expression databases
Bgeei | ENSRNOG00000030483, Expressed in skeletal muscle tissue and 22 other tissues |
Genevisiblei | Q08602, RN |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR032675, LRR_dom_sf IPR002088, Prenyl_trans_a IPR036254, RabGGT_asu_insert-dom_sf IPR009087, RabGGT_asu_insert-domain IPR032955, RabGGTase_alpha |
PANTHERi | PTHR11129:SF2, PTHR11129:SF2, 1 hit |
Pfami | View protein in Pfam PF01239, PPTA, 5 hits PF07711, RabGGT_insert, 1 hit |
SUPFAMi | SSF49594, SSF49594, 1 hit |
PROSITEi | View protein in PROSITE PS51147, PFTA, 6 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PGTA_RAT | |
Accessioni | Q08602Primary (citable) accession number: Q08602 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families