UniProtKB - Q08495 (DEMA_HUMAN)
Dematin
DMTN
Functioni
GO - Molecular functioni
- actin binding Source: UniProtKB
- actin filament binding Source: GO_Central
- protein self-association Source: UniProtKB
- signaling receptor binding Source: UniProtKB
- spectrin binding Source: UniProtKB
GO - Biological processi
- actin cytoskeleton organization Source: UniProtKB
- actin filament bundle assembly Source: UniProtKB
- actin filament capping Source: UniProtKB-KW
- actin filament reorganization Source: UniProtKB
- calcium-mediated signaling using extracellular calcium source Source: UniProtKB
- calcium-mediated signaling using intracellular calcium source Source: UniProtKB
- cellular response to calcium ion Source: UniProtKB
- cellular response to cAMP Source: UniProtKB
- cytoskeleton organization Source: UniProtKB
- erythrocyte development Source: UniProtKB
- lamellipodium assembly Source: GO_Central
- negative regulation of cell-substrate adhesion Source: UniProtKB
- negative regulation of focal adhesion assembly Source: UniProtKB
- negative regulation of peptidyl-serine phosphorylation Source: UniProtKB
- negative regulation of peptidyl-threonine phosphorylation Source: UniProtKB
- negative regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
- negative regulation of protein targeting to membrane Source: UniProtKB
- negative regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
- positive regulation of blood coagulation Source: UniProtKB
- positive regulation of fibroblast migration Source: UniProtKB
- positive regulation of integrin-mediated signaling pathway Source: UniProtKB
- positive regulation of platelet aggregation Source: UniProtKB
- positive regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
- positive regulation of wound healing Source: UniProtKB
- protein-containing complex assembly Source: UniProtKB
- protein secretion by platelet Source: UniProtKB
- regulation of actin cytoskeleton organization Source: UniProtKB
- regulation of cell shape Source: UniProtKB
- regulation of filopodium assembly Source: UniProtKB
- regulation of lamellipodium assembly Source: UniProtKB
- transmembrane transport Source: Reactome
Keywordsi
Molecular function | Actin capping, Actin-binding |
Enzyme and pathway databases
PathwayCommonsi | Q08495 |
Reactomei | R-HSA-5223345, Miscellaneous transport and binding events |
Names & Taxonomyi
Protein namesi | Recommended name: DematinAlternative name(s): Dematin actin-binding protein Erythrocyte membrane protein band 4.9 |
Gene namesi | Name:DMTN Synonyms:DMT, EPB49 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:3382, DMTN |
MIMi | 125305, gene |
neXtProti | NX_Q08495 |
VEuPathDBi | HostDB:ENSG00000158856.17 |
Subcellular locationi
Cytoskeleton
Cytoplasm and Cytosol
Plasma membrane
Other locations
- perinuclear region By similarity
- Membrane By similarity
- Endomembrane system
- Cell projection By similarity
Note: Localized at the spectrin-actin junction of erythrocyte plasma membrane. Localized to intracellular membranes and the cytoskeletal network. Localized at intracellular membrane-bounded organelle compartment in platelets that likely represent the dense tubular network membrane. Detected at the cell membrane and at the parasitophorous vacuole in malaria-infected erythrocytes at late stages of plasmodium berghei or falciparum development.
Cytoskeleton
- actin cytoskeleton Source: GO_Central
- actin filament Source: UniProtKB
- cortical cytoskeleton Source: Ensembl
- spectrin-associated cytoskeleton Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB
Plasma Membrane
- cell projection membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- cytoplasmic vesicle Source: UniProtKB
- endomembrane system Source: UniProtKB-SubCell
- perinuclear region of cytoplasm Source: UniProtKB
- platelet dense tubular network membrane Source: UniProtKB
- postsynaptic density Source: Ensembl
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 124 | S → A: Reduces interaction with plasmodium berghei 14-3-3 protein. Inhibits phosphorylation and interaction with plasmodium berghei 14-3-3 protein; when associated with A-333 and A-403. 1 Publication | 1 | |
Mutagenesisi | 333 | S → A: Reduces interaction with plasmodium berghei 14-3-3 protein. Inhibits phosphorylation and interaction with plasmodium berghei 14-3-3 protein; when associated with A-124 and A-403. 1 Publication | 1 | |
Mutagenesisi | 403 | S → A: Inhibits phosphorylation and interaction with plasmodium berghei 14-3-3 protein; when associated with A-124 and A-333. 2 Publications | 1 | |
Mutagenesisi | 403 | S → E: Reduces F-actin bundling but not F-actin binding activity. 2 Publications | 1 |
Keywords - Diseasei
Tumor suppressorOrganism-specific databases
DisGeNETi | 2039 |
OpenTargetsi | ENSG00000158856 |
PharmGKBi | PA27815 |
Miscellaneous databases
Pharosi | Q08495, Tbio |
Genetic variation databases
BioMutai | DMTN |
DMDMi | 22654240 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218755 | 1 – 405 | DematinAdd BLAST | 405 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 16 | PhosphoserineCombined sources | 1 | |
Modified residuei | 18 | PhosphoserineCombined sources | 1 | |
Modified residuei | 26 | PhosphoserineCombined sources | 1 | |
Modified residuei | 92 | PhosphoserineCombined sources | 1 | |
Modified residuei | 96 | PhosphoserineCombined sources | 1 | |
Modified residuei | 105 | PhosphoserineCombined sources | 1 | |
Modified residuei | 110 | PhosphoserineBy similarity | 1 | |
Modified residuei | 113 | PhosphoserineBy similarity | 1 | |
Modified residuei | 156 | PhosphoserineCombined sources | 1 | |
Modified residuei | 226 | PhosphoserineCombined sources | 1 | |
Modified residuei | 269 | PhosphoserineCombined sources | 1 | |
Modified residuei | 279 | PhosphoserineCombined sources | 1 | |
Modified residuei | 289 | PhosphoserineCombined sources | 1 | |
Modified residuei | 303 | PhosphoserineCombined sources | 1 | |
Modified residuei | 315 | PhosphoserineBy similarity | 1 | |
Modified residuei | 333 | PhosphoserineCombined sources | 1 | |
Modified residuei | 372 | PhosphoserineCombined sources | 1 | |
Modified residuei | 383 | PhosphoserineCombined sources | 1 | |
Modified residuei | 403 | Phosphoserine; by PKA3 Publications | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
EPDi | Q08495 |
jPOSTi | Q08495 |
MassIVEi | Q08495 |
MaxQBi | Q08495 |
PaxDbi | Q08495 |
PeptideAtlasi | Q08495 |
PRIDEi | Q08495 |
ProteomicsDBi | 19903 4281 58618 [Q08495-1] 58619 [Q08495-2] |
PTM databases
iPTMneti | Q08495 |
PhosphoSitePlusi | Q08495 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000158856, Expressed in right frontal lobe and 228 other tissues |
ExpressionAtlasi | Q08495, baseline and differential |
Genevisiblei | Q08495, HS |
Organism-specific databases
HPAi | ENSG00000158856, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Monomeric (isoform 2); under reducing conditions. Self-associates. Exists under oxidizing condition as a trimer of two isoforms 2 and isoform 1 linked by disulfide bonds (Probable).
Found in a complex with DMTN, F-actin and spectrin.
Found in a complex with ADD2, DMTN and SLC2A1.
Interacts with F-actin, ITPKB, RASGRF2 and spectrin.
Isoform 2 interacts with SLC2A1 (via C-terminus cytoplasmic region). Isoform 1 and isoform 2 interact (phosphorylated form) with plasmodium berghei 14-3-3 protein; the interaction occurs in a PKA-dependent manner.
Curated8 PublicationsBinary interactionsi
Hide detailsQ08495
With | #Exp. | IntAct |
---|---|---|
CEP76 [Q8TAP6] | 3 | EBI-715275,EBI-742887 |
GOLGA2 [Q08379] | 3 | EBI-715275,EBI-618309 |
TEX11 - isoform 3 [Q8IYF3-3] | 3 | EBI-715275,EBI-11523345 |
TRAF2 [Q12933] | 3 | EBI-715275,EBI-355744 |
GO - Molecular functioni
- actin binding Source: UniProtKB
- actin filament binding Source: GO_Central
- protein self-association Source: UniProtKB
- signaling receptor binding Source: UniProtKB
- spectrin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 108353, 25 interactors |
CORUMi | Q08495 |
IntActi | Q08495, 16 interactors |
STRINGi | 9606.ENSP00000427866 |
Miscellaneous databases
RNActi | Q08495, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q08495 |
SMRi | Q08495 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q08495 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 337 – 405 | HPPROSITE-ProRule annotationAdd BLAST | 69 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 224 – 308 | Interaction with RASGRF21 PublicationAdd BLAST | 85 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 216 – 222 | Poly-Glu | 7 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1044, Eukaryota |
GeneTreei | ENSGT00950000182850 |
HOGENOMi | CLU_001357_12_1_1 |
InParanoidi | Q08495 |
OMAi | DWLENRS |
OrthoDBi | 1083894at2759 |
PhylomeDBi | Q08495 |
TreeFami | TF318042 |
Family and domain databases
Gene3Di | 1.10.950.10, 1 hit |
InterProi | View protein in InterPro IPR032402, AbLIM_anchor IPR003128, Villin_headpiece IPR036886, Villin_headpiece_dom_sf |
Pfami | View protein in Pfam PF16182, AbLIM_anchor, 1 hit PF02209, VHP, 1 hit |
SMARTi | View protein in SMART SM00153, VHP, 1 hit |
SUPFAMi | SSF47050, SSF47050, 1 hit |
PROSITEi | View protein in PROSITE PS51089, HP, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MERLQKQPLT SPGSVSPSRD SSVPGSPSSI VAKMDNQVLG YKDLAAIPKD
60 70 80 90 100
KAILDIERPD LMIYEPHFTY SLLEHVELPR SRERSLSPKS TSPPPSPEVW
110 120 130 140 150
ADSRSPGIIS QASAPRTTGT PRTSLPHFHH PETSRPDSNI YKKPPIYKQR
160 170 180 190 200
ESVGGSPQTK HLIEDLIIES SKFPAAQPPD PNQPAKIETD YWPCPPSLAV
210 220 230 240 250
VETEWRKRKA SRRGAEEEEE EEDDDSGEEM KALRERQREE LSKVTSNLGK
260 270 280 290 300
MILKEEMEKS LPIRRKTRSL PDRTPFHTSL HQGTSKSSSL PAYGRTTLSR
310 320 330 340 350
LQSTEFSPSG SETGSPGLQN GEGQRGRMDR GNSLPCVLEQ KIYPYEMLVV
360 370 380 390 400
TNKGRTKLPP GVDRMRLERH LSAEDFSRVF AMSPEEFGKL ALWKRNELKK
KASLF
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE5RFK4 | E5RFK4_HUMAN | Dematin | DMTN | 218 | Annotation score: | ||
E5RGQ7 | E5RGQ7_HUMAN | Dematin | DMTN | 148 | Annotation score: | ||
E5RJ61 | E5RJ61_HUMAN | Dematin | DMTN | 110 | Annotation score: | ||
A0A087WT94 | A0A087WT94_HUMAN | Dematin | DMTN | 71 | Annotation score: | ||
E5RJC0 | E5RJC0_HUMAN | Dematin | DMTN | 162 | Annotation score: | ||
E5RGQ0 | E5RGQ0_HUMAN | Dematin | DMTN | 201 | Annotation score: | ||
A0A087WUC0 | A0A087WUC0_HUMAN | Dematin | DMTN | 73 | Annotation score: | ||
E5RFK6 | E5RFK6_HUMAN | Dematin | DMTN | 71 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 81 | S → Q in AAA58438 (PubMed:8341682).Curated | 1 | |
Sequence conflicti | 81 | S → Q in AAC50223 (PubMed:7615546).Curated | 1 | |
Sequence conflicti | 155 | G → A in BAG52385 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 292 | A → R in AAA58438 (PubMed:8341682).Curated | 1 | |
Sequence conflicti | 292 | A → R in AAC50223 (PubMed:7615546).Curated | 1 | |
Sequence conflicti | 347 | M → V in AAA58438 (PubMed:8341682).Curated | 1 | |
Sequence conflicti | 380 | F → S in AAA58438 (PubMed:8341682).Curated | 1 | |
Sequence conflicti | 403 | S → P in BAG52385 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044803 | 7 – 31 | Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST | 25 | |
Alternative sequenceiVSP_057428 | 83 – 97 | Missing in isoform 4. 1 PublicationAdd BLAST | 15 | |
Alternative sequenceiVSP_004189 | 320 – 341 | Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST | 22 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QZP | NMR | - | A | 342-405 | [»] | |
1ZV6 | NMR | - | A | 342-405 | [»] | |
BMRBi | Q08495 | |||||
SMRi | Q08495 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108353, 25 interactors |
CORUMi | Q08495 |
IntActi | Q08495, 16 interactors |
STRINGi | 9606.ENSP00000427866 |
PTM databases
iPTMneti | Q08495 |
PhosphoSitePlusi | Q08495 |
Genetic variation databases
BioMutai | DMTN |
DMDMi | 22654240 |
Proteomic databases
EPDi | Q08495 |
jPOSTi | Q08495 |
MassIVEi | Q08495 |
MaxQBi | Q08495 |
PaxDbi | Q08495 |
PeptideAtlasi | Q08495 |
PRIDEi | Q08495 |
ProteomicsDBi | 19903 4281 58618 [Q08495-1] 58619 [Q08495-2] |
Protocols and materials databases
Antibodypediai | 9250, 243 antibodies |
DNASUi | 2039 |
Genome annotation databases
Organism-specific databases
CTDi | 2039 |
DisGeNETi | 2039 |
GeneCardsi | DMTN |
HGNCi | HGNC:3382, DMTN |
HPAi | ENSG00000158856, Tissue enhanced (brain) |
MIMi | 125305, gene |
neXtProti | NX_Q08495 |
OpenTargetsi | ENSG00000158856 |
PharmGKBi | PA27815 |
VEuPathDBi | HostDB:ENSG00000158856.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1044, Eukaryota |
GeneTreei | ENSGT00950000182850 |
HOGENOMi | CLU_001357_12_1_1 |
InParanoidi | Q08495 |
OMAi | DWLENRS |
OrthoDBi | 1083894at2759 |
PhylomeDBi | Q08495 |
TreeFami | TF318042 |
Enzyme and pathway databases
PathwayCommonsi | Q08495 |
Reactomei | R-HSA-5223345, Miscellaneous transport and binding events |
Miscellaneous databases
BioGRID-ORCSi | 2039, 6 hits in 956 CRISPR screens |
ChiTaRSi | DMTN, human |
EvolutionaryTracei | Q08495 |
GeneWikii | EPB49 |
GenomeRNAii | 2039 |
Pharosi | Q08495, Tbio |
PROi | PR:Q08495 |
RNActi | Q08495, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000158856, Expressed in right frontal lobe and 228 other tissues |
ExpressionAtlasi | Q08495, baseline and differential |
Genevisiblei | Q08495, HS |
Family and domain databases
Gene3Di | 1.10.950.10, 1 hit |
InterProi | View protein in InterPro IPR032402, AbLIM_anchor IPR003128, Villin_headpiece IPR036886, Villin_headpiece_dom_sf |
Pfami | View protein in Pfam PF16182, AbLIM_anchor, 1 hit PF02209, VHP, 1 hit |
SMARTi | View protein in SMART SM00153, VHP, 1 hit |
SUPFAMi | SSF47050, SSF47050, 1 hit |
PROSITEi | View protein in PROSITE PS51089, HP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DEMA_HUMAN | |
Accessioni | Q08495Primary (citable) accession number: Q08495 Secondary accession number(s): A8K0T5 Q9BRE3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | August 30, 2002 | |
Last modified: | April 7, 2021 | |
This is version 205 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families