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Entry version 191 (12 Aug 2020)
Sequence version 2 (21 Dec 2004)
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Protein

Cytochrome P450 4F3

Gene

CYP4F3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and their oxygenated derivatives (oxylipins) (PubMed:8486631, PubMed:9675028, PubMed:11461919, PubMed:15145985, PubMed:16547005, PubMed:16820285, PubMed:18182499, PubMed:18065749, PubMed:18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). May play a role in inactivation of proinflammatory and anti-inflammatory oxylipins during the resolution of inflammation (PubMed:8486631, PubMed:9675028, PubMed:11461919, PubMed:15145985, PubMed:15364545, PubMed:16547005, PubMed:16820285, PubMed:18182499, PubMed:18065749, PubMed:18577768).10 Publications
Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of oxylipins in myeloid cells, displaying higher affinity for arachidonate metabolite leukotriene B4 (LTB4) (PubMed:8486631, PubMed:9675028, PubMed:11461919, PubMed:15364545). Inactivates LTB4 via three successive oxidative transformations to 20-hydroxy-LTB4, then to 20-oxo-LTB4 and to 20-carboxy-LTB4 (PubMed:9675028). Exhibits omega-hydroxylase activity toward long-chain fatty acid epoxides with preference for 8,9-epoxy-(5Z,11Z,14Z)-eicosatrienoate (EET) and 9,10-epoxyoctadecanoate (PubMed:15145985). Omega-hydroxylates monohydroxy polyunsaturated fatty acids (PUFAs), including hydroxyeicosatetraenoates (HETEs) and hydroxyeicosapentaenoates (HEPEs), to dihydroxy compounds (PubMed:15364545, PubMed:9675028). Contributes to the degradation of saturated very long-chain fatty acids (VLCFAs) such as docosanoic acid, by catalyzing successive omega-oxidations to the corresponding dicarboxylic acid, thereby initiating chain shortening (PubMed:18182499). Has low hydroxylase activity toward PUFAs (PubMed:18577768, PubMed:11461919).7 Publications
Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of polyunsaturated fatty acids (PUFAs) (PubMed:11461919, PubMed:16820285, PubMed:18577768). Participates in the conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure (PubMed:11461919, PubMed:16820285, PubMed:18577768). Exhibits high omega-hydroxylase activity toward other PUFAs, including eicosatrienoic acid (ETA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (PubMed:16820285, PubMed:18577768). Can also catalyze the oxidation of the penultimate carbon (omega-1 oxidation) of PUFAs with lower efficiency (PubMed:18577768). Contributes to the degradation of saturated very long-chain fatty acids (VLCFAs) such as docosanoic acid and hexacosanoic acid, by catalyzing successive omega-oxidations and to the corresponding dicarboxylic acids, thereby initiating chain shortening (PubMed:16547005, PubMed:18182499). Omega-hydroxylates long-chain 3-hydroxy fatty acids, likely initiating the oxidative conversion to the corresponding 3-hydroxydicarboxylic fatty acids (PubMed:18065749). Has omega-hydroxylase activity toward long-chain fatty acid epoxides with preference for 8,9-epoxy-(5Z,11Z,14Z)-eicosatrienoate (EET) and 9,10-epoxyoctadecanoate (PubMed:15145985). Has lower affinity for leukotriene B4 (LTB4) compared with CYP4F3A, yet may contribute to the inactivation of LTB4 in liver (PubMed:15145985).7 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

hemeBy similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by carbon monoxide (CO).1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

The omega-hydroxylation of VLCFAs follows dual-enzyme Michaelis-Menten kinetics, suggesting simultaneous binding of two substrate molecules. The high affinity Michaelis-Menten constants are shown.1 Publication
  1. KM=0.64 µM for leukotriene B4 (isoform CYP4F3A)1 Publication
  2. KM=6.5 µM for 20-hydroxy-leukotriene B4 (isoform CYP4F3A)1 Publication
  3. KM=17.9 µM for lipoxin B4 (isoform CYP4F3A)1 Publication
  4. KM=25.7 µM for 5-HETE (isoform CYP4F3A)1 Publication
  5. KM=40.9 µM for 12-HETE (isoform CYP4F3A)1 Publication
  6. KM=49.5 µM for 5-HEPE (isoform CYP4F3A)1 Publication
  7. KM=14.2 µM for 12-hydroxyoctadecanoate (isoform CYP4F3A)1 Publication
  8. KM=22.8 µM for (12R)-hydroxy-(9Z)-octadecenoate (isoform CYP4F3A)1 Publication
  9. KM=0.68 µM for leukotriene B4 (isoform CYP4F3A)1 Publication
  10. KM=20.6 µM for leukotriene B4 (isoform CYP4F3B)1 Publication
  11. KM=185.6 µM for arachidonate (isoform CYP4F3A)1 Publication
  12. KM=22.0 µM for arachidonate (isoform CYP4F3B)1 Publication
  13. KM=6.3 µM for 9(10)-epoxyoctadecanoate (isoform CYP4F3A)1 Publication
  14. KM=35.7 µM for 9(10)-epoxyoctadecanoate (isoform CYP4F3B)1 Publication
  15. KM=46.6 µM for 9(10)-epoxy-(12Z)-octadecenoate (isoform CYP4F3A)1 Publication
  16. KM=108.1 µM for 9(10)-epoxy-(12Z)-octadecenoate (isoform CYP4F3B)1 Publication
  17. KM=61.8 µM for 12(13)-epoxy-(9Z)-octadecenoate (isoform CYP4F3A)1 Publication
  18. KM=100.6 µM for 12(13)-epoxy-(9Z)-octadecenoate (isoform CYP4F3B)1 Publication
  19. KM=1.6 µM for docosanoate (isoform CYP4F3B)1 Publication
  20. KM=3.8 µM for tetracosanoate (isoform CYP4F3B)1 Publication
  21. KM=1.3 µM for hexacosanoate (isoform CYP4F3B)1 Publication
  22. KM=0.6 µM for 22-hydroxydocosanoate (isoform CYP4F3A)1 Publication
  23. KM=13.1 µM for 22-hydroxydocosanoate (isoform CYP4F3B)1 Publication
  24. KM=5.2 µM for 26-hydroxyhexacosanoate (isoform CYP4F3B)1 Publication
  1. Vmax=34.0 nmol/min/nmol enzyme toward leukotriene B41 Publication
  2. Vmax=66.7 nmol/min/nmol enzyme toward 20-hydroxy-leukotriene B41 Publication
  3. Vmax=38.5 nmol/min/nmol enzyme toward lipoxin B4 (isoform CYP4F3A)1 Publication
  4. Vmax=21.6 nmol/min/nmol enzyme toward 5-HETE (isoform CYP4F3A)1 Publication
  5. Vmax=43.2 nmol/min/nmol enzyme toward 12-HETE (isoform CYP4F3A)1 Publication
  6. Vmax=95.1 nmol/min/nmol enzyme toward 5-HEPE (isoform CYP4F3A)1 Publication
  7. Vmax=54.5 nmol/min/nmol enzyme toward 12-hydroxyoctadecanoate (isoform CYP4F3A)1 Publication
  8. Vmax=19.0 nmol/min/nmol enzyme toward (12R)-hydroxy-(9Z)-octadecenoate1 Publication
  9. Vmax=32.8 pmol/min/pmol enzyme toward leukotriene B4 (isoform CYP4F3A)1 Publication
  10. Vmax=23.3 pmol/min/pmol enzyme toward leukotriene B4 (isoform CYP4F3B)1 Publication
  11. Vmax=11.5 pmol/min/pmol enzyme toward arachidonate (isoform CYP4F3A)1 Publication
  12. Vmax=13.3 pmol/min/pmol enzyme toward arachidonate (isoform CYP4F3B)1 Publication
  13. Vmax=10.8 nmol/min/nmol enzyme toward 9(10)-epoxyoctadecanoate (isoform CYP4F3A)1 Publication
  14. Vmax=13.2 nmol/min/nmol enzyme toward 9(10)-epoxyoctadecanoate (isoform CYP4F3B)1 Publication
  15. Vmax=21.2 nmol/min/nmol enzyme toward 9(10)-epoxy-(12Z)-octadecenoate (isoform CYP4F3A)1 Publication
  16. Vmax=15.02 nmol/min/nmol enzyme toward 9(10)-epoxy-(12Z)-octadecenoate (isoform CYP4F3B)1 Publication
  17. Vmax=3 nmol/min/nmol enzyme toward 12(13)-epoxy-(9Z)-octadecenoate (isoform CYP4F3A)1 Publication
  18. Vmax=3.93 nmol/min/nmol enzyme toward 12(13)-epoxy-(9Z)-octadecenoate (isoform CYP4F3B)1 Publication
  19. Vmax=5.0 pmol/min/pmol enzyme toward docosanoate (isoform CYP4F3B)1 Publication
  20. Vmax=9.8 pmol/min/pmol enzyme toward tetracosanoate (isoform CYP4F3B)1 Publication
  21. Vmax=2.2 pmol/min/pmol enzyme toward hexacosanoate (isoform CYP4F3B)1 Publication
  22. Vmax=0.2 pmol/min/pmol enzyme toward 22-hydroxydocosanoate (isoform CYP4F3A)1 Publication
  23. Vmax=0.3 pmol/min/pmol enzyme toward 22-hydroxydocosanoate (isoform CYP4F3B)1 Publication
  24. Vmax=0.8 pmol/min/pmol enzyme toward 26-hydroxyhexacosanoate (isoform CYP4F3B)1 Publication

pH dependencei

Optimum pH is 7.5.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: leukotriene B4 degradation

This protein is involved in the pathway leukotriene B4 degradation, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway leukotriene B4 degradation and in Lipid metabolism.

Pathwayi: arachidonate metabolism

This protein is involved in the pathway arachidonate metabolism, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway arachidonate metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei328Heme (covalent; via 1 link)By similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi468Iron (heme axial ligand)By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processFatty acid metabolism, Lipid metabolism
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

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PathwayCommonsi
Q08477

Reactome - a knowledgebase of biological pathways and processes

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Reactomei
R-HSA-211935, Fatty acids
R-HSA-211958, Miscellaneous substrates
R-HSA-211979, Eicosanoids
R-HSA-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX)

SABIO-RK: Biochemical Reaction Kinetics Database

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SABIO-RKi
Q08477

UniPathway: a resource for the exploration and annotation of metabolic pathways

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UniPathwayi
UPA00383
UPA00883

Chemistry databases

SwissLipids knowledge resource for lipid biology

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SwissLipidsi
SLP:000000422 [Q08477-1]
SLP:000000423 [Q08477-2]

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Cytochrome P450 4F3 (EC:1.14.14.13 Publications)
Alternative name(s):
20-hydroxyeicosatetraenoic acid synthase1 Publication
Short name:
20-HETE synthase1 Publication
CYPIVF3
Cytochrome P450-LTB-omega
Docosahexaenoic acid omega-hydroxylase CYP4F31 Publication (EC:1.14.14.791 Publication)
Leukotriene-B(4) 20-monooxygenase 2
Leukotriene-B(4) omega-hydroxylase 2 (EC:1.14.14.942 Publications)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:CYP4F31 PublicationImported
Synonyms:LTB4H
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 19

Organism-specific databases

Eukaryotic Pathogen Database Resources

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EuPathDBi
HostDB:ENSG00000186529.14

Human Gene Nomenclature Database

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HGNCi
HGNC:2646, CYP4F3

Online Mendelian Inheritance in Man (OMIM)

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MIMi
601270, gene

neXtProt; the human protein knowledge platform

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neXtProti
NX_Q08477

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei11 – 31HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

DisGeNET

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DisGeNETi
4051

Open Targets

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OpenTargetsi
ENSG00000186529

The Pharmacogenetics and Pharmacogenomics Knowledge Base

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PharmGKBi
PA234

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

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Pharosi
Q08477, Tbio

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

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ChEMBLi
CHEMBL3508692

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

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BioMutai
CYP4F3

Domain mapping of disease mutations (DMDM)

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DMDMi
56757430

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000518511 – 520Cytochrome P450 4F3Add BLAST520

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q08477

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
Q08477

MaxQB - The MaxQuant DataBase

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MaxQBi
Q08477

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
Q08477

PeptideAtlas

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PeptideAtlasi
Q08477

PRoteomics IDEntifications database

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PRIDEi
Q08477

ProteomicsDB: a multi-organism proteome resource

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ProteomicsDBi
58614 [Q08477-1]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
Q08477

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
Q08477

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Isoform CYP4F3A is expressed in the polymorphonuclear leukocytes as well as leukocytes and bone marrow. Isoform CYP4F3B is selectively expressed in liver and kidney and is also the predominant CYP4F isoform in trachea and tissues of the gastrointestinal tract.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000186529, Expressed in right lobe of liver and 148 other tissues

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q08477, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q08477, HS

Organism-specific databases

Human Protein Atlas

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HPAi
ENSG00000186529, Tissue enriched (liver)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Protein interaction database and analysis system

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IntActi
Q08477, 4 interactors

STRING: functional protein association networks

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STRINGi
9606.ENSP00000221307

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

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RNActi
Q08477, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q08477

Database of comparative protein structure models

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ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG0157, Eukaryota

Ensembl GeneTree

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GeneTreei
ENSGT00940000163407

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q08477

KEGG Orthology (KO)

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KOi
K17726

Identification of Orthologs from Complete Genome Data

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OMAi
IAWNISG

Database for complete collections of gene phylogenies

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PhylomeDBi
Q08477

TreeFam database of animal gene trees

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TreeFami
TF105088

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
1.10.630.10, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR001128, Cyt_P450
IPR017972, Cyt_P450_CS
IPR002401, Cyt_P450_E_grp-I
IPR036396, Cyt_P450_sf

Pfam protein domain database

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Pfami
View protein in Pfam
PF00067, p450, 1 hit

Protein Motif fingerprint database; a protein domain database

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PRINTSi
PR00463, EP450I
PR00385, P450

Superfamily database of structural and functional annotation

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SUPFAMi
SSF48264, SSF48264, 1 hit

PROSITE; a protein domain and family database

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PROSITEi
View protein in PROSITE
PS00086, CYTOCHROME_P450, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform CYP4F3A (identifier: Q08477-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPQLSLSSLG LWPMAASPWL LLLLVGASWL LARILAWTYT FYDNCCRLRC
60 70 80 90 100
FPQPPKRNWF LGHLGLIHSS EEGLLYTQSL ACTFGDMCCW WVGPWHAIVR
110 120 130 140 150
IFHPTYIKPV LFAPAAIVPK DKVFYSFLKP WLGDGLLLSA GEKWSRHRRM
160 170 180 190 200
LTPAFHFNIL KPYMKIFNES VNIMHAKWQL LASEGSARLD MFEHISLMTL
210 220 230 240 250
DSLQKCVFSF DSHCQEKPSE YIAAILELSA LVTKRHQQIL LYIDFLYYLT
260 270 280 290 300
PDGQRFRRAC RLVHDFTDAV IQERRRTLPS QGVDDFLQAK AKSKTLDFID
310 320 330 340 350
VLLLSKDEDG KKLSDEDIRA EADTFMFEGH DTTASGLSWV LYHLAKHPEY
360 370 380 390 400
QERCRQEVQE LLKDREPKEI EWDDLAQLPF LTMCIKESLR LHPPVPAVSR
410 420 430 440 450
CCTQDIVLPD GRVIPKGIIC LISVFGTHHN PAVWPDPEVY DPFRFDPKNI
460 470 480 490 500
KERSPLAFIP FSAGPRNCIG QAFAMAEMKV VLGLTLLRFR VLPDHTEPRR
510 520
KPELVLRAEG GLWLRVEPLS
Length:520
Mass (Da):59,847
Last modified:December 21, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i2519D875280CF9DC
GO
Isoform CYP4F3B (identifier: Q08477-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-114: IHSSEEGLLY...TYIKPVLFAP → VTPTEQGMRV...NIIRSVINAS

Show »
Length:520
Mass (Da):59,739
Checksum:iEA737D889AD662C2
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
V9GYA1V9GYA1_HUMAN
Cytochrome P450 4F3
CYP4F3
74Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
K7EJZ8K7EJZ8_HUMAN
Cytochrome P450 4F3
CYP4F3
79Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti184 – 186EGS → KGY in AAC08589 (Ref. 3) Curated3
Sequence conflicti488R → A in BAA02144 (PubMed:8486631).Curated1
Sequence conflicti488R → A in BAA25990 (PubMed:9539102).Curated1
Sequence conflicti488R → A in BAA25991 (PubMed:9539102).Curated1
Sequence conflicti512L → I in AAC08589 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_04845796H → Q. Corresponds to variant dbSNP:rs34923393Ensembl.1
Natural variantiVAR_048458106Y → C. Corresponds to variant dbSNP:rs35888783Ensembl.1
Natural variantiVAR_001258269A → D3 PublicationsCorresponds to variant dbSNP:rs1805040Ensembl.1
Natural variantiVAR_020664270V → I1 PublicationCorresponds to variant dbSNP:rs28371536Ensembl.1
Natural variantiVAR_020665271I → T1 PublicationCorresponds to variant dbSNP:rs28371479Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_04719367 – 114IHSSE…VLFAP → VTPTEQGMRVLTQLVATYPQ GFKVWMGPIFPVIRFCHPNI IRSVINAS in isoform CYP4F3B. 3 PublicationsAdd BLAST48

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
D12620 mRNA Translation: BAA02144.1
D12621 mRNA Translation: BAA02145.1
AB002454 mRNA Translation: BAA25990.1
AB002461 Genomic DNA Translation: BAA25991.1
AF054821 mRNA Translation: AAC08589.1
AK304200 mRNA Translation: BAH14129.1
AK316136 mRNA Translation: BAH14507.1
AY792513 Genomic DNA Translation: AAV40834.1
AD000685 Genomic DNA No translation available.
CH471106 Genomic DNA Translation: EAW84489.1
BC136299 mRNA Translation: AAI36300.1

The Consensus CDS (CCDS) project

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CCDSi
CCDS12332.1 [Q08477-1]
CCDS59362.1 [Q08477-2]

Protein sequence database of the Protein Information Resource

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PIRi
A46661

NCBI Reference Sequences

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RefSeqi
NP_000887.2, NM_000896.2 [Q08477-1]
NP_001186137.1, NM_001199208.1 [Q08477-2]
NP_001186138.1