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Protein

20S-pre-rRNA D-site endonuclease NOB1

Gene

NOB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Required for the synthesis of 40S ribosome subunits. Has a role in processing 20S pre-rRNA into the mature 18S rRNA, where it is required for cleavage at the 3' end of the mature 18S rRNA (D-site). Accompanies the 20S pre-rRNA from the nucleus to the cytoplasm. In association with NIN1, may promote the recruitment of the proteasome to the ribosomal subunits stalled in maturation.2 Publications

Miscellaneous

Present with 4490 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi304ZincBy similarity1
Metal bindingi307ZincBy similarity1
Metal bindingi323ZincBy similarity1
Metal bindingi326ZincBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Nuclease
Biological processRibosome biogenesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-33596-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
20S-pre-rRNA D-site endonuclease NOB1 (EC:3.1.-.-)
Alternative name(s):
NIN1-binding protein
Pre-rRNA-processing endonuclease NOB1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NOB1
Ordered Locus Names:YOR056C
ORF Names:YOR29-07
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XV

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000005582 NOB1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi92D → N: No 20S cleavage. 1 Publication1
Mutagenesisi110D → N: No change in activity or growth. 1 Publication1
Mutagenesisi279L → R: Temperature-sensitive. 1 Publication1
Mutagenesisi280Q → G: Temperature-sensitive. 1 Publication1
Mutagenesisi281M → G: Temperature-sensitive. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002705541 – 45920S-pre-rRNA D-site endonuclease NOB1Add BLAST459

Proteomic databases

MaxQB - The MaxQuant DataBase

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MaxQBi
Q08444

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q08444

PRoteomics IDEntifications database

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PRIDEi
Q08444

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
Q08444

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the small ribosomal subunit, ribosomal RNA processing complex (SSU RRP complex). Interacts with PNO1.2 Publications

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
34454, 230 interactors

Database of interacting proteins

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DIPi
DIP-6440N

Protein interaction database and analysis system

More...
IntActi
Q08444, 35 interactors

Molecular INTeraction database

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MINTi
Q08444

STRING: functional protein association networks

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STRINGi
4932.YOR056C

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

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ProteinModelPortali
Q08444

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q08444

Database of comparative protein structure models

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ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini10 – 115PINcAdd BLAST106

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the NOB1 family.Curated

Phylogenomic databases

Ensembl GeneTree

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GeneTreei
ENSGT00390000015857

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
HOG000159692

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q08444

KEGG Orthology (KO)

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KOi
K11883

Identification of Orthologs from Complete Genome Data

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OMAi
KVISLTY

Database of Orthologous Groups

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OrthoDBi
EOG092C221B

Family and domain databases

Conserved Domains Database

More...
CDDi
cd09876 PIN_Nob1, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.40.120, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR039907 NOB1
IPR017117 Nob1_euk
IPR036283 NOB1_Zf-like_sf
IPR014881 NOB1_Zn-bd
IPR002716 PIN_dom
IPR039909 PIN_NOB1
IPR033411 Ribonuclease_PIN

The PANTHER Classification System

More...
PANTHERi
PTHR12814 PTHR12814, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF08772 NOB1_Zn_bind, 1 hit
PF17146 PIN_6, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF037125 D-site_20S_pre-rRNA_nuclease, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00670 PINc, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF144206 SSF144206, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q08444-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTENQTAHVR ALILDATPLI TQSYTHYQNY AQSFYTTPTV FQEIKDAQAR
60 70 80 90 100
KNLEIWQSLG TLKLVHPSEN SIAKVSTFAK LTGDYSVLSA NDLHILALTY
110 120 130 140 150
ELEIKLNNGD WRLRKKPGDA LDASKADVGT DGKQKLTEDN KKEEDSESVP
160 170 180 190 200
KKKNKRRGGK KQKAKREARE AREAENANLE LESKAEEHVE EAGSKEQICN
210 220 230 240 250
DENIKESSDL NEVFEDADDD GDWITPENLT EAIIKDSGED TTGSLGVEAS
260 270 280 290 300
EEDRHVALNR PENQVALATG DFAVQNVALQ MNLNLMNFMS GLKIKRIRNY
310 320 330 340 350
MLRCHACFKI FPLPKDGKPK HFCASCGGQG TLLRCAVSVD SRTGNVTPHL
360 370 380 390 400
KSNFQWNNRG NRYSVASPLS KNSQKRYGKK GHVHSKPQEN VILREDQKEY
410 420 430 440 450
EKVIKQEEWT RRHNEKILNN WIGGGSADNY ISPFAITGLK QHNVRIGKGR

YVNSSKRRS
Length:459
Mass (Da):51,742
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i96DEB818BB490A98
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Z70678 Genomic DNA Translation: CAA94541.1
Z74964 Genomic DNA Translation: CAA99249.1
BK006948 Genomic DNA Translation: DAA10835.1

Protein sequence database of the Protein Information Resource

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PIRi
S66939

NCBI Reference Sequences

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RefSeqi
NP_014699.1, NM_001183475.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YOR056C_mRNA; YOR056C_mRNA; YOR056C

Database of genes from NCBI RefSeq genomes

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GeneIDi
854221

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YOR056C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA Translation: CAA94541.1
Z74964 Genomic DNA Translation: CAA99249.1
BK006948 Genomic DNA Translation: DAA10835.1
PIRiS66939
RefSeqiNP_014699.1, NM_001183475.1

3D structure databases

ProteinModelPortaliQ08444
SMRiQ08444
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34454, 230 interactors
DIPiDIP-6440N
IntActiQ08444, 35 interactors
MINTiQ08444
STRINGi4932.YOR056C

PTM databases

iPTMnetiQ08444

Proteomic databases

MaxQBiQ08444
PaxDbiQ08444
PRIDEiQ08444

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR056C_mRNA; YOR056C_mRNA; YOR056C
GeneIDi854221
KEGGisce:YOR056C

Organism-specific databases

SGDiS000005582 NOB1

Phylogenomic databases

GeneTreeiENSGT00390000015857
HOGENOMiHOG000159692
InParanoidiQ08444
KOiK11883
OMAiKVISLTY
OrthoDBiEOG092C221B

Enzyme and pathway databases

BioCyciYEAST:G3O-33596-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q08444

Family and domain databases

CDDicd09876 PIN_Nob1, 1 hit
Gene3Di3.30.40.120, 1 hit
InterProiView protein in InterPro
IPR039907 NOB1
IPR017117 Nob1_euk
IPR036283 NOB1_Zf-like_sf
IPR014881 NOB1_Zn-bd
IPR002716 PIN_dom
IPR039909 PIN_NOB1
IPR033411 Ribonuclease_PIN
PANTHERiPTHR12814 PTHR12814, 1 hit
PfamiView protein in Pfam
PF08772 NOB1_Zn_bind, 1 hit
PF17146 PIN_6, 1 hit
PIRSFiPIRSF037125 D-site_20S_pre-rRNA_nuclease, 1 hit
SMARTiView protein in SMART
SM00670 PINc, 1 hit
SUPFAMiSSF144206 SSF144206, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNOB1_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q08444
Secondary accession number(s): D6W2B9, O00021
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: December 5, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names
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