UniProtKB - Q08436 (PMA3_NICPL)
Protein
Plasma membrane ATPase 3
Gene
PMA3
Organism
Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Status
Functioni
The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
Catalytic activityi
- EC:7.1.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 333 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 592 | MagnesiumBy similarity | 1 | |
Metal bindingi | 596 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- proton-exporting ATPase activity, phosphorylative mechanism Source: UniProtKB-EC
GO - Biological processi
- proton export across plasma membrane Source: InterPro
Keywordsi
Molecular function | Translocase |
Biological process | Hydrogen ion transport, Ion transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PMA3 |
Organismi | Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco) |
Taxonomic identifieri | 4092 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana |
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 65 | CytoplasmicSequence analysisAdd BLAST | 65 | |
Transmembranei | 66 – 85 | Helical; Name=1Sequence analysisAdd BLAST | 20 | |
Topological domaini | 86 – 97 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 98 – 118 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 119 – 247 | CytoplasmicSequence analysisAdd BLAST | 129 | |
Transmembranei | 248 – 268 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 269 – 278 | ExtracellularSequence analysis | 10 | |
Transmembranei | 279 – 300 | Helical; Name=4Sequence analysisAdd BLAST | 22 | |
Topological domaini | 301 – 647 | CytoplasmicSequence analysisAdd BLAST | 347 | |
Transmembranei | 648 – 669 | Helical; Name=5Sequence analysisAdd BLAST | 22 | |
Topological domaini | 670 – 674 | ExtracellularSequence analysis | 5 | |
Transmembranei | 675 – 697 | Helical; Name=6Sequence analysisAdd BLAST | 23 | |
Topological domaini | 698 – 713 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 714 – 734 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 735 – 759 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 760 – 780 | Helical; Name=8Sequence analysisAdd BLAST | 21 | |
Topological domaini | 781 – 792 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 793 – 813 | Helical; Name=9Sequence analysisAdd BLAST | 21 | |
Topological domaini | 814 – 822 | ExtracellularSequence analysis | 9 | |
Transmembranei | 823 – 843 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Topological domaini | 844 – 956 | CytoplasmicSequence analysisAdd BLAST | 113 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046292 | 1 – 956 | Plasma membrane ATPase 3Add BLAST | 956 |
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | Q08436 |
Expressioni
Tissue specificityi
Expressed in roots, stems, leaves from both vegetative and flowering plants, and flowers at early and late stages of development with highest expression levels found in flowers and root tissue.
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q08436 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q08436 |
Family & Domainsi
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q08436-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGEKPEVLDA VLKETVDLEN IPIEEVFENL RCTKEGLTAT AAQERLSIFG
60 70 80 90 100
YNKLEEKKES KFSKFLGFMW NPLSWVMEAA AIMAIALANG GGKPPDWQDF
110 120 130 140 150
VGIITLLIIN STISFIEENN AGNAAAALMA RLAPKAKVLR DGKWKEEDAA
160 170 180 190 200
VLVPGDIISI KLGDIIPADA RLLEGDPLKI DQSALTGESL PVTKGPGDGV
210 220 230 240 250
YSGSTCKQGE IEAVVIATGV HTFFGKAAHL VDSTNQVGHF QKVLTAIGNF
260 270 280 290 300
CICSIAVGMI IEIIVMYPIQ HRKYRPGIDN LLVLLIGGIP IAMPTVLSVT
310 320 330 340 350
MAIGSHRLAQ QGAITKRMTA IEEMAGMDVL CSDKTGTLTL NKLTVDKYLI
360 370 380 390 400
EVFARGVDAD TVVLMAARAS RTENQDAIDA AIVGMLADPK EARAGIREIH
410 420 430 440 450
FLPFNPTDKR TALTYLDGEG KMHRVSKGAP EQILHLAHNK SDIERRVHAV
460 470 480 490 500
IDKFAERGLR SLAVAYQEVP EGRKESAGGP WQFIALLPLF DPPRHDSAET
510 520 530 540 550
IRRALNLGVN VKMITGDQLA IGKETGRRLG MGTNMYPSSA LLGQTKDESI
560 570 580 590 600
SALPVDELIE KADGFAGVFP EHKYEIVKRL QARKHICGMT GDGVNDAPAL
610 620 630 640 650
KKADIGIAVD DATDAARSAS DIVLTEPGLS VIISAVLTSR AIFQRMKNYT
660 670 680 690 700
IYAVSITIRI VLGFMLLALI WQFDFPPFMV LIIAILNDGT IMTISKDRVK
710 720 730 740 750
PSPLPDSWKL AEIFTTGVVL GGYLAMMTVI FFWAAYKTNF FPRVFGVSTL
760 770 780 790 800
EKTATDDFRK LASAIYLQVS TISQALIFVT RSRSWSFMER PGLLLVVAFF
810 820 830 840 850
IAQLVATLIA VYANWSFAAI EGIGWGWAGV IWLYNIVFYI PLDLXXFLIR
860 870 880 890 900
YALSGKAWDL VIEQRIAFTR KKDFGKEQRE LQWAHAQRTL HGLQVPDPKI
910 920 930 940 950
FSETTNFNEL NQLAEEAKRR AEIARLRELH TLKGHVESVV KLKGLDIETI
QQAYTV
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 63 | S → L. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M80490 mRNA Translation: AAA34098.1 M80491 Genomic DNA Translation: AAA34099.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M80490 mRNA Translation: AAA34098.1 M80491 Genomic DNA Translation: AAA34099.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2O98 | X-ray | 2.70 | P/Q | 905-956 | [»] | |
SMRi | Q08436 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PRIDEi | Q08436 |
Miscellaneous databases
EvolutionaryTracei | Q08436 |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PMA3_NICPL | |
Accessioni | Q08436Primary (citable) accession number: Q08436 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | December 11, 2019 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families