UniProtKB - Q08379 (GOGA2_HUMAN)
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Protein
Golgin subfamily A member 2
Gene
GOLGA2
Organism
Homo sapiens (Human)
Status
Functioni
Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).By similarity6 Publications
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
- identical protein binding Source: IntAct
- importin-alpha family protein binding Source: UniProtKB
- microtubule binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
- syntaxin binding Source: UniProtKB
GO - Biological processi
- asymmetric cell division Source: UniProtKB
- centrosome cycle Source: UniProtKB
- COPII vesicle coating Source: Reactome
- ER to Golgi vesicle-mediated transport Source: UniProtKB
- Golgi disassembly Source: UniProtKB
- Golgi ribbon formation Source: UniProtKB
- microtubule nucleation Source: UniProtKB
- mitotic spindle assembly Source: UniProtKB
- negative regulation of autophagy Source: ParkinsonsUK-UCL
- negative regulation of protein binding Source: UniProtKB
- positive regulation of protein glycosylation Source: ParkinsonsUK-UCL
- positive regulation of ubiquitin protein ligase activity Source: Reactome
- protein glycosylation Source: UniProtKB
- protein homotetramerization Source: UniProtKB
- spindle assembly Source: UniProtKB
- spindle assembly involved in meiosis Source: UniProtKB
Keywordsi
| Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
| Reactomei | R-HSA-162658 Golgi Cisternae Pericentriolar Stack Reorganization R-HSA-204005 COPII-mediated vesicle transport R-HSA-6807878 COPI-mediated anterograde transport R-HSA-8862803 Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models |
| SIGNORi | Q08379 |
Protein family/group databases
| MoonDBi | Q08379 Predicted |
Names & Taxonomyi
| Protein namesi | Recommended name: Golgin subfamily A member 2Alternative name(s): 130 kDa cis-Golgi matrix protein1 Publication Short name: GM1301 Publication GM130 autoantigen1 Publication Golgin-951 Publication |
| Gene namesi | Name:GOLGA2 |
| Organismi | Homo sapiens (Human) |
| Taxonomic identifieri | 9606 [NCBI] |
| Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
| Proteomesi |
|
Organism-specific databases
| EuPathDBi | HostDB:ENSG00000167110.16 |
| HGNCi | HGNC:4425 GOLGA2 |
| MIMi | 602580 gene |
| neXtProti | NX_Q08379 |
Subcellular locationi
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, MicrotubulePathology & Biotechi
Mutagenesis
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Mutagenesisi | 18 | R → K: Impaired methylation; when associated with R-30 and R-35. 1 Publication | 1 | |
| Mutagenesisi | 26 – 49 | KKKLR…KKKKK → AAALAEYQQANSPGVPTGAA AAAA: Abolishes interaction with importin-alpha. 1 PublicationAdd BLAST | 24 | |
| Mutagenesisi | 30 | R → K: Impaired methylation; when associated with R-18 and R-35. 1 Publication | 1 | |
| Mutagenesisi | 35 | R → K: Impaired methylation; when associated with R-18 and R-35. 1 Publication | 1 | |
| Mutagenesisi | 37 | S → D: Phosphomimetic mutant. Does not affect interaction with importin-alpha. 1 Publication | 1 |
Organism-specific databases
| DisGeNETi | 2801 |
| OpenTargetsi | ENSG00000167110 |
| PharmGKBi | PA28805 |
Polymorphism and mutation databases
| BioMutai | GOLGA2 |
| DMDMi | 294862511 |
PTM / Processingi
Molecule processing
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| ChainiPRO_0000190054 | 1 – 1002 | Golgin subfamily A member 2Add BLAST | 1002 |
Amino acid modifications
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Modified residuei | 18 | Omega-N-methylated arginine1 Publication | 1 | |
| Modified residuei | 30 | Dimethylated arginine1 Publication | 1 | |
| Modified residuei | 35 | Dimethylated arginine1 Publication | 1 | |
| Modified residuei | 37 | PhosphoserineCombined sources1 Publication | 1 | |
| Modified residuei | 66 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 273 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 438 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 690 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 937 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 953 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 981 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Cleaved by caspases at the onset of apoptosis.1 Publication
Methylation by PRMT5 is required for Golgi ribbon formation. While dimethylation at Arg-30 and Arg-35 are confirmed in vivo, it is unclear whether Arg-18 is methylated in vivo.1 Publication
Phosphorylated at Ser-37 by CDK1 at the onset of mitosis, inhibiting the interaction with p115/USO1 and triggering Golgi disassembly (PubMed:20421892, PubMed:26165940). Phosphorylated at Ser-37 in prophase as the Golgi complex starts to break down, and remains phosphorylated during further breakdown and partitioning of the Golgi fragments in metaphase and anaphase. In telophase, GM130 is dephosphorylated by PP2A as the Golgi fragments start to reassemble (By similarity).By similarity2 Publications
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
| EPDi | Q08379 |
| MaxQBi | Q08379 |
| PaxDbi | Q08379 |
| PeptideAtlasi | Q08379 |
| PRIDEi | Q08379 |
| ProteomicsDBi | 58604 58605 [Q08379-2] |
PTM databases
| iPTMneti | Q08379 |
| PhosphoSitePlusi | Q08379 |
Expressioni
Gene expression databases
| Bgeei | ENSG00000167110 |
| CleanExi | HS_GOLGA2 |
| ExpressionAtlasi | Q08379 baseline and differential |
| Genevisiblei | Q08379 HS |
Organism-specific databases
| HPAi | CAB015421 HPA021178 HPA021230 HPA021799 |
Interactioni
Subunit structurei
Part of a larger oligomeric complex (By similarity). Interacts with p115/USO1 (By similarity). Interacts with RAB1B that has been activated by GTP-binding (PubMed:11306556). Interacts with GORASP1/GRASP65 (PubMed:16489344, PubMed:18045989). Interacts with ZFPL1 (PubMed:18323775). Interacts with AKAP450/AKAP9; leading to recruit AKAP450/AKAP9 to the cis-Golgi (PubMed:19242490).By similarity5 Publications
Binary interactionsi
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
- identical protein binding Source: IntAct
- importin-alpha family protein binding Source: UniProtKB
- microtubule binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
- syntaxin binding Source: UniProtKB
Protein-protein interaction databases
| BioGridi | 109063, 373 interactors |
| CORUMi | Q08379 |
| DIPi | DIP-34271N |
| IntActi | Q08379, 551 interactors |
| MINTi | Q08379 |
| STRINGi | 9606.ENSP00000416097 |
Structurei
3D structure databases
| Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
| 4REY | X-ray | 1.96 | B | 980-1002 | [»] | |
| ProteinModelPortali | Q08379 | |||||
| SMRi | Q08379 | |||||
| ModBasei | Search... | |||||
| MobiDBi | Search... | |||||
Family & Domainsi
Region
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Regioni | 1 – 84 | Interaction with p115/USO1By similarityAdd BLAST | 84 | |
| Regioni | 992 – 1002 | Interaction with GORASP1/GRASP65By similarityAdd BLAST | 11 |
Coiled coil
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Coiled coili | 16 – 892 | Sequence analysisAdd BLAST | 877 |
Motif
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Motifi | 26 – 49 | Nuclear localization signal1 PublicationAdd BLAST | 24 |
Compositional bias
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Compositional biasi | 46 – 51 | Poly-Lys | 6 | |
| Compositional biasi | 449 – 455 | Poly-Pro | 7 | |
| Compositional biasi | 704 – 715 | Poly-GluAdd BLAST | 12 |
Domaini
The nuclear localization signal (cNLS) mediates interaction with importin-alpha, recruiting importin-alpha to the Golgi membrane and liberating TPX2.1 Publication
Sequence similaritiesi
Belongs to the GOLGA2 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
| eggNOGi | ENOG410INYK Eukaryota ENOG4111MFD LUCA |
| GeneTreei | ENSGT00530000062932 |
| HOGENOMi | HOG000218631 |
| HOVERGENi | HBG051752 |
| InParanoidi | Q08379 |
| KOi | K20358 |
| OMAi | TQRRMLK |
| OrthoDBi | EOG091G0DDT |
| PhylomeDBi | Q08379 |
| TreeFami | TF316990 |
Family and domain databases
| InterProi | View protein in InterPro IPR024858 Golgin_A |
| Pfami | View protein in Pfam PF15070 GOLGA2L5, 1 hit |
Sequences (2)i
Sequence statusi: Complete.
This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q08379-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MWPQPRLPPR PAMSEETRQS KLAAAKKKLR EYQQRNSPGV PTGAKKKKKI
60 70 80 90 100
KNGSNPETTT SGGCHSPEDT PKDNAATLQP SDDTVLPGGV PSPGASLTSM
110 120 130 140 150
AASQNHDADN VPNLMDETKT FSSTESLRQL SQQLNGLVCE SATCVNGEGP
160 170 180 190 200
ASSANLKDLE SRYQQLAVAL DSSYVTNKQL NITIEKLKQQ NQEITDQLEE
210 220 230 240 250
EKKECHQKQG ALREQLQVHI QTIGILVSEK AELQTALAHT QHAARQKEGE
260 270 280 290 300
SEDLASRLQY SRRRVGELER ALSAVSTQQK KADRYNKELT KERDALRLEL
310 320 330 340 350
YKNTQSNEDL KQEKSELEEK LRVLVTEKAG MQLNLEELQK KLEMTELLLQ
360 370 380 390 400
QFSSRCEAPD ANQQLQQAME ERAQLEAHLG QVMESVRQLQ MERDKYAENL
410 420 430 440 450
KGESAMWRQR MQQMSEQVHT LREEKECSMS RVQELETSLA ELRNQMAEPP
460 470 480 490 500
PPEPPAGPSE VEQQLQAEAE HLRKELEGLA GQLQAQVQDN EGLSRLNREQ
510 520 530 540 550
EERLLELERA AELWGEQAEA RRQILETMQN DRTTISRALS QNRELKEQLA
560 570 580 590 600
ELQSGFVKLT NENMEITSAL QSEQHVKREL GKKLGELQEK LSELKETVEL
610 620 630 640 650
KSQEAQSLQQ QRDQYLGHLQ QYVAAYQQLT SEKEVLHNQL LLQTQLVDQL
660 670 680 690 700
QQQEAQGKAV AEMARQELQE TQERLEAATQ QNQQLRAQLS LMAHPGEGDG
710 720 730 740 750
LDREEEEDEE EEEEEAVAVP QPMPSIPEDL ESREAMVAFF NSAVASAEEE
760 770 780 790 800
QARLRGQLKE QRVRCRRLAH LLASAQKEPE AAAPAPGTGG DSVCGETHRA
810 820 830 840 850
LQGAMEKLQS RFMELMQEKA DLKERVEELE HRCIQLSGET DTIGEYIALY
860 870 880 890 900
QSQRAVLKER HREKEEYISR LAQDKEEMKV KLLELQELVL RLVGDRNEWH
910 920 930 940 950
GRFLAAAQNP ADEPTSGAPA PQELGAANQQ GDLCEVSLAG SVEPAQGEAR
960 970 980 990 1000
EGSPRDNPTA QQIMQLLREM QNPRERPGLG SNPCIPFFYR ADENDEVKIT
VI
Sequence cautioni
The sequence AAH69268 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAP35912 differs from that shown. Sequence differs from that shown after position 826 due to an internal deletion.Curated
The sequence EAW87762 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Sequence conflicti | 709 | Missing in AAP35912 (Ref. 6) Curated | 1 | |
| Sequence conflicti | 762 | R → S in AAP35912 (Ref. 6) Curated | 1 |
Natural variant
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Natural variantiVAR_033974 | 902 | R → G. Corresponds to variant dbSNP:rs2240961Ensembl. | 1 |
Alternative sequence
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Alternative sequenceiVSP_007727 | 1 – 382 | Missing in isoform 2. 1 PublicationAdd BLAST | 382 |
Sequence databases
| Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06147 mRNA Translation: AAA35920.1 AL590708 Genomic DNA No translation available. CH471090 Genomic DNA Translation: EAW87762.1 Sequence problems. BC014188 mRNA Translation: AAH14188.2 BC069268 mRNA Translation: AAH69268.1 Different initiation. AF248953 mRNA Translation: AAF65550.1 BT007248 mRNA Translation: AAP35912.1 Sequence problems. |
| CCDSi | CCDS6896.2 [Q08379-1] |
| PIRi | JH0821 |
| RefSeqi | NP_004477.3, NM_004486.4 [Q08379-1] |
| UniGenei | Hs.155827 |
Genome annotation databases
| Ensembli | ENST00000421699; ENSP00000416097; ENSG00000167110 [Q08379-1] ENST00000611957; ENSP00000478799; ENSG00000167110 [Q08379-1] |
| GeneIDi | 2801 |
| KEGGi | hsa:2801 |
| UCSCi | uc011maw.2 human [Q08379-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Entry informationi
| Entry namei | GOGA2_HUMAN | |
| Accessioni | Q08379Primary (citable) accession number: Q08379 Secondary accession number(s): A0A0C4DGS5 Q9NYF9 | |
| Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
| Last sequence update: | April 20, 2010 | |
| Last modified: | June 20, 2018 | |
| This is version 172 of the entry and version 3 of the sequence. See complete history. | ||
| Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
| Annotation program | Chordata Protein Annotation Program | |
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families
