UniProtKB - Q08369 (GATA4_MOUSE)
Transcription factor GATA-4
Gata4
Functioni
Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (By similarity).
In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression (By similarity).
Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity).
Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity).
Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (PubMed:9584153).
Promotes cardiac myocyte enlargement (By similarity).
Required during testicular development (By similarity).
May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase (By similarity).
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 216 – 240 | GATA-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 270 – 294 | GATA-type 2PROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- chromatin binding Source: MGI
- cis-regulatory region sequence-specific DNA binding Source: MGI
- co-SMAD binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription activator activity Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
- DNA-binding transcription factor binding Source: MGI
- NFAT protein binding Source: MGI
- protein kinase binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: BHF-UCL
- RNA polymerase II-specific DNA-binding transcription factor binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription cis-regulatory region binding Source: MGI
- transcription coactivator binding Source: BHF-UCL
- zinc ion binding Source: InterPro
GO - Biological processi
- aortic valve morphogenesis Source: MGI
- atrial septum morphogenesis Source: MGI
- atrial septum primum morphogenesis Source: BHF-UCL
- atrial septum secundum morphogenesis Source: MGI
- atrioventricular canal development Source: BHF-UCL
- atrioventricular valve formation Source: BHF-UCL
- atrioventricular valve morphogenesis Source: MGI
- cardiac muscle cell differentiation Source: MGI
- cardiac muscle hypertrophy in response to stress Source: MGI
- cardiac muscle tissue development Source: MGI
- cardiac muscle tissue regeneration Source: BHF-UCL
- cardiac right ventricle morphogenesis Source: BHF-UCL
- cardiac septum development Source: BHF-UCL
- cell-cell signaling Source: MGI
- cell fate commitment Source: GO_Central
- cell growth involved in cardiac muscle cell development Source: MGI
- cellular response to follicle-stimulating hormone stimulus Source: MGI
- cellular response to gonadotropin stimulus Source: MGI
- diaphragm morphogenesis Source: BHF-UCL
- embryonic digestive tract morphogenesis Source: MGI
- embryonic foregut morphogenesis Source: MGI
- embryonic heart tube anterior/posterior pattern specification Source: MGI
- embryonic heart tube development Source: MGI
- embryonic morphogenesis Source: MGI
- endocardial cushion development Source: BHF-UCL
- endoderm formation Source: MGI
- epithelial cell fate commitment Source: MGI
- gastrulation with mouth forming second Source: MGI
- heart development Source: MGI
- heart looping Source: BHF-UCL
- heart morphogenesis Source: BHF-UCL
- intestinal epithelial cell differentiation Source: MGI
- in utero embryonic development Source: MGI
- lung lobe formation Source: MGI
- lung morphogenesis Source: BHF-UCL
- male gonad development Source: MGI
- mitral valve formation Source: BHF-UCL
- negative regulation of apoptotic process Source: BHF-UCL
- negative regulation of apoptotic signaling pathway Source: BHF-UCL
- negative regulation of autophagy Source: MGI
- negative regulation of cardiac muscle cell apoptotic process Source: BHF-UCL
- negative regulation of connective tissue replacement Source: BHF-UCL
- negative regulation of gene expression Source: BHF-UCL
- negative regulation of oxidative stress-induced cell death Source: BHF-UCL
- negative regulation of transcription by RNA polymerase II Source: GO_Central
- outflow tract morphogenesis Source: BHF-UCL
- positive regulation of angiogenesis Source: BHF-UCL
- positive regulation of BMP signaling pathway Source: MGI
- positive regulation of cardiac muscle cell proliferation Source: MGI
- positive regulation of cardioblast differentiation Source: BHF-UCL
- positive regulation of cell cycle Source: BHF-UCL
- positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
- positive regulation of gene expression Source: BHF-UCL
- positive regulation of protein phosphorylation Source: BHF-UCL
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of vascular endothelial growth factor production Source: BHF-UCL
- regulation of cardiac muscle cell contraction Source: MGI
- regulation of cardiac muscle cell proliferation Source: MGI
- regulation of gene expression Source: MGI
- regulation of protein kinase B signaling Source: BHF-UCL
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- response to estrogen Source: MGI
- response to mechanical stimulus Source: MGI
- response to retinoic acid Source: BHF-UCL
- response to xenobiotic stimulus Source: MGI
- seminiferous tubule development Source: MGI
- Sertoli cell differentiation Source: MGI
- signal transduction involved in regulation of gene expression Source: MGI
- skeletal muscle cell differentiation Source: UniProtKB
- SMAD protein signal transduction Source: MGI
- spermatogenesis Source: MGI
- transdifferentiation Source: MGI
- tricuspid valve formation Source: BHF-UCL
- tube morphogenesis Source: MGI
- vasculogenesis involved in coronary vascular morphogenesis Source: DFLAT
- ventricular cardiac muscle tissue development Source: MGI
- ventricular septum development Source: BHF-UCL
- wound healing Source: BHF-UCL
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor GATA-4Alternative name(s): GATA-binding factor 4 |
Gene namesi | Name:Gata4 Synonyms:Gata-4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95664, Gata4 |
VEuPathDBi | HostDB:ENSMUSG00000021944 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nuclear body Source: MGI
- nucleoplasm Source: Reactome
- nucleus Source: BHF-UCL
- RNA polymerase II transcription regulator complex Source: MGI
Other locations
- chromatin Source: BHF-UCL
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083414 | 1 – 441 | Transcription factor GATA-4Add BLAST | 441 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 299 | N6-methyllysine; by EZH21 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
MethylationProteomic databases
MaxQBi | Q08369 |
PaxDbi | Q08369 |
PRIDEi | Q08369 |
ProteomicsDBi | 271190 |
PTM databases
iPTMneti | Q08369 |
PhosphoSitePlusi | Q08369 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000021944, Expressed in heart and 145 other tissues |
ExpressionAtlasi | Q08369, baseline and differential |
Genevisiblei | Q08369, MM |
Interactioni
Subunit structurei
Interacts with ZNF260 (By similarity).
Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger.
Also interacts with JARID2 which represses its ability to activate transcription of ANF.
Interacts (via the second Zn finger) with NFATC4 (PubMed:9568714).
Interacts with LMCD1 (PubMed:16199866).
Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes.
Interacts with NR5A1, ZFPM2 and TBX5.
Interacts with TBX18.
By similarity5 PublicationsBinary interactionsi
Q08369
With | #Exp. | IntAct |
---|---|---|
Jarid2 [Q62315] | 3 | EBI-297008,EBI-493592 |
Nkx2-5 [P42582] | 4 | EBI-297008,EBI-297021 |
TBX5 [Q99593] from Homo sapiens. | 2 | EBI-297008,EBI-297043 |
GO - Molecular functioni
- co-SMAD binding Source: MGI
- DNA-binding transcription factor binding Source: MGI
- NFAT protein binding Source: MGI
- protein kinase binding Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: MGI
- transcription coactivator binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 199841, 17 interactors |
DIPi | DIP-33032N |
IntActi | Q08369, 3 interactors |
MINTi | Q08369 |
STRINGi | 10090.ENSMUSP00000066927 |
Chemistry databases
BindingDBi | Q08369 |
Miscellaneous databases
RNActi | Q08369, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 60 – 107 | DisorderedSequence analysisAdd BLAST | 48 | |
Regioni | 313 – 392 | DisorderedSequence analysisAdd BLAST | 80 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 60 – 90 | Polar residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 339 – 363 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 372 – 392 | Polar residuesSequence analysisAdd BLAST | 21 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 216 – 240 | GATA-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 270 – 294 | GATA-type 2PROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1601, Eukaryota |
GeneTreei | ENSGT00940000158349 |
HOGENOMi | CLU_027524_0_0_1 |
InParanoidi | Q08369 |
PhylomeDBi | Q08369 |
Family and domain databases
CDDi | cd00202, ZnF_GATA, 2 hits |
Gene3Di | 3.30.50.10, 2 hits |
InterProi | View protein in InterPro IPR008013, GATA_N IPR016375, TF_GATA_4/5/6 IPR039355, Transcription_factor_GATA IPR000679, Znf_GATA IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR10071, PTHR10071, 1 hit |
Pfami | View protein in Pfam PF00320, GATA, 2 hits PF05349, GATA-N, 1 hit |
PIRSFi | PIRSF003028, TF_GATA_4/5/6, 1 hit |
PRINTSi | PR00619, GATAZNFINGER |
SMARTi | View protein in SMART SM00401, ZnF_GATA, 2 hits |
PROSITEi | View protein in PROSITE PS00344, GATA_ZN_FINGER_1, 2 hits PS50114, GATA_ZN_FINGER_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MYQSLAMAAN HGPPPGAYEA GGPGAFMHSA GAASSPVYVP TPRVPSSVLG
60 70 80 90 100
LSYLQGGGSA AAAGTTSGGS SGAGPSGAGP GTQQGSPGWS QAGAEGAAYT
110 120 130 140 150
PPPVSPRFSF PGTTGSLAAA AAAAAAREAA AYGSGGGAAG AGLAGREQYG
160 170 180 190 200
RPGFAGSYSS PYPAYMADVG ASWAAAAAAS AGPFDSPVLH SLPGRANPGR
210 220 230 240 250
HPNLDMFDDF SEGRECVNCG AMSTPLWRRD GTGHYLCNAC GLYHKMNGIN
260 270 280 290 300
RPLIKPQRRL SASRRVGLSC ANCQTTTTTL WRRNAEGEPV CNACGLYMKL
310 320 330 340 350
HGVPRPLAMR KEGIQTRKRK PKNLNKSKTP AGPAGETLPP SSGASSGNSS
360 370 380 390 400
NATSSSSSSE EMRPIKTEPG LSSHYGHSSS MSQTFSTVSG HGPSIHPVLS
410 420 430 440
ALKLSPQGYA SPVTQTSQAS SKQDSWNSLV LADSHGDIIT A
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PXW9 | E9PXW9_MOUSE | Transcription factor GATA-4 | Gata4 | 442 | Annotation score: | ||
Q3UYJ1 | Q3UYJ1_MOUSE | Transcription factor GATA-4 | Gata4 | 236 | Annotation score: | ||
A4K4Z6 | A4K4Z6_MOUSE | Transcription factor GATA-4 | Gata4 gata4 | 11 | Annotation score: | ||
D3YTQ2 | D3YTQ2_MOUSE | Transcription factor GATA-4 | Gata4 | 15 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 385 – 402 | FSTVS…VLSAL → SVCVRPRALHPSSAVCS in AAA37662 (PubMed:8455608).CuratedAdd BLAST | 18 | |
Sequence conflicti | 385 – 402 | FSTVS…VLSAL → SVCVRPRALHPSSAVCS in AAB42015 (Ref. 3) CuratedAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M98339 mRNA Translation: AAA37662.1 Frameshift. U85046 mRNA Translation: AAB42015.1 AF179424 mRNA Translation: AAD55266.1 AC090654 Genomic DNA No translation available. AC090962 Genomic DNA No translation available. CH466535 Genomic DNA Translation: EDL36067.1 BC137824 mRNA Translation: AAI37825.1 |
CCDSi | CCDS49519.1 |
PIRi | A48099 |
RefSeqi | NP_032118.2, NM_008092.4 |
Genome annotation databases
Ensembli | ENSMUST00000118022; ENSMUSP00000113891; ENSMUSG00000021944 |
GeneIDi | 14463 |
KEGGi | mmu:14463 |
UCSCi | uc007uhn.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M98339 mRNA Translation: AAA37662.1 Frameshift. U85046 mRNA Translation: AAB42015.1 AF179424 mRNA Translation: AAD55266.1 AC090654 Genomic DNA No translation available. AC090962 Genomic DNA No translation available. CH466535 Genomic DNA Translation: EDL36067.1 BC137824 mRNA Translation: AAI37825.1 |
CCDSi | CCDS49519.1 |
PIRi | A48099 |
RefSeqi | NP_032118.2, NM_008092.4 |
3D structure databases
AlphaFoldDBi | Q08369 |
SMRi | Q08369 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199841, 17 interactors |
DIPi | DIP-33032N |
IntActi | Q08369, 3 interactors |
MINTi | Q08369 |
STRINGi | 10090.ENSMUSP00000066927 |
Chemistry databases
BindingDBi | Q08369 |
ChEMBLi | CHEMBL1687680 |
PTM databases
iPTMneti | Q08369 |
PhosphoSitePlusi | Q08369 |
Proteomic databases
MaxQBi | Q08369 |
PaxDbi | Q08369 |
PRIDEi | Q08369 |
ProteomicsDBi | 271190 |
Protocols and materials databases
Antibodypediai | 3405, 758 antibodies from 45 providers |
DNASUi | 14463 |
Genome annotation databases
Ensembli | ENSMUST00000118022; ENSMUSP00000113891; ENSMUSG00000021944 |
GeneIDi | 14463 |
KEGGi | mmu:14463 |
UCSCi | uc007uhn.1, mouse |
Organism-specific databases
CTDi | 2626 |
MGIi | MGI:95664, Gata4 |
VEuPathDBi | HostDB:ENSMUSG00000021944 |
Phylogenomic databases
eggNOGi | KOG1601, Eukaryota |
GeneTreei | ENSGT00940000158349 |
HOGENOMi | CLU_027524_0_0_1 |
InParanoidi | Q08369 |
PhylomeDBi | Q08369 |
Enzyme and pathway databases
Reactomei | R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
BioGRID-ORCSi | 14463, 7 hits in 75 CRISPR screens |
PROi | PR:Q08369 |
RNActi | Q08369, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021944, Expressed in heart and 145 other tissues |
ExpressionAtlasi | Q08369, baseline and differential |
Genevisiblei | Q08369, MM |
Family and domain databases
CDDi | cd00202, ZnF_GATA, 2 hits |
Gene3Di | 3.30.50.10, 2 hits |
InterProi | View protein in InterPro IPR008013, GATA_N IPR016375, TF_GATA_4/5/6 IPR039355, Transcription_factor_GATA IPR000679, Znf_GATA IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR10071, PTHR10071, 1 hit |
Pfami | View protein in Pfam PF00320, GATA, 2 hits PF05349, GATA-N, 1 hit |
PIRSFi | PIRSF003028, TF_GATA_4/5/6, 1 hit |
PRINTSi | PR00619, GATAZNFINGER |
SMARTi | View protein in SMART SM00401, ZnF_GATA, 2 hits |
PROSITEi | View protein in PROSITE PS00344, GATA_ZN_FINGER_1, 2 hits PS50114, GATA_ZN_FINGER_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | GATA4_MOUSE | |
Accessioni | Q08369Primary (citable) accession number: Q08369 Secondary accession number(s): B9EHF7, P97491, Q9QZK4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | March 19, 2014 | |
Last modified: | May 25, 2022 | |
This is version 188 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot