UniProtKB - Q08297 (RAD51_MOUSE)
DNA repair protein RAD51 homolog 1
Rad51
Functioni
Miscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 127 – 134 | ATPBy similarity | 8 |
GO - Molecular functioni
- ATP binding Source: MGI
- chromatin binding Source: MGI
- DNA-dependent ATPase activity Source: GO_Central
- DNA polymerase binding Source: MGI
- double-stranded DNA binding Source: UniProtKB
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- protein C-terminus binding Source: MGI
- recombinase activity Source: GO_Central
- single-stranded DNA binding Source: MGI
- single-stranded DNA helicase activity Source: UniProtKB
GO - Biological processi
- cellular response to alkaloid Source: MGI
- cellular response to camptothecin Source: UniProtKB
- cellular response to cisplatin Source: Ensembl
- cellular response to DNA damage stimulus Source: UniProtKB
- cellular response to gamma radiation Source: Ensembl
- cellular response to hydroxyurea Source: MGI
- cellular response to ionizing radiation Source: UniProtKB
- chromosome organization involved in meiotic cell cycle Source: MGI
- DNA recombinase assembly Source: UniProtKB
- DNA repair Source: UniProtKB
- DNA unwinding involved in DNA replication Source: UniProtKB
- double-strand break repair via homologous recombination Source: MGI
- interstrand cross-link repair Source: UniProtKB
- meiotic cell cycle Source: MGI
- mitotic recombination Source: GO_Central
- mitotic recombination-dependent replication fork processing Source: InterPro
- positive regulation of DNA ligation Source: MGI
- reciprocal meiotic recombination Source: GO_Central
- regulation of double-strand break repair via homologous recombination Source: UniProtKB
- regulation of protein phosphorylation Source: MGI
- replication-born double-strand break repair via sister chromatid exchange Source: MGI
- replication fork processing Source: MGI
- response to drug Source: Ensembl
- response to glucoside Source: Ensembl
- response to ionizing radiation Source: GO_Central
- response to toxic substance Source: Ensembl
- response to X-ray Source: Ensembl
- strand invasion Source: GO_Central
- telomere maintenance via recombination Source: BHF-UCL
- telomere maintenance via telomere lengthening Source: BHF-UCL
- telomere organization Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA recombination, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-5685938, HDR through Single Strand Annealing (SSA) R-MMU-5685942, HDR through Homologous Recombination (HRR) R-MMU-5693568, Resolution of D-loop Structures through Holliday Junction Intermediates R-MMU-5693579, Homologous DNA Pairing and Strand Exchange R-MMU-5693616, Presynaptic phase of homologous DNA pairing and strand exchange |
Names & Taxonomyi
Protein namesi | Recommended name: DNA repair protein RAD51 homolog 1CuratedAlternative name(s): RAD51 homolog A |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97890, Rad51 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
Nucleus
- Nucleus By similarity
Mitochondrion
- Mitochondrion matrix By similarity
Other locations
- Cytoplasm By similarity
- perinuclear region By similarity
- Chromosome 2 Publications
Note: Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner. Recruited at sites of DNA damage in a MCM9-MCM8-dependent manner.By similarity
Cytoskeleton
- microtubule organizing center Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial matrix Source: UniProtKB-SubCell
- mitochondrion Source: MGI
Nucleus
- condensed nuclear chromosome Source: MGI
- lateral element Source: MGI
- nuclear chromatin Source: MGI
- nuclear chromosome Source: UniProtKB
- nuclear chromosome, telomeric region Source: BHF-UCL
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
- PML body Source: MGI
- synaptonemal complex Source: UniProtKB
Other locations
- chromatin Source: MGI
- condensed chromosome Source: MGI
- cytoplasm Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
- protein-containing complex Source: MGI
- site of double-strand break Source: MGI
Keywords - Cellular componenti
Chromosome, Cytoplasm, Cytoskeleton, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000122933 | 2 – 339 | DNA repair protein RAD51 homolog 1Add BLAST | 338 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 54 | Phosphotyrosine; by ABL1By similarity | 1 | |
Cross-linki | 58 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 64 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 309 | Phosphothreonine; by CHEK1By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q08297 |
MaxQBi | Q08297 |
PaxDbi | Q08297 |
PRIDEi | Q08297 |
PTM databases
iPTMneti | Q08297 |
PhosphoSitePlusi | Q08297 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000027323, Expressed in ear vesicle and 237 other tissues |
ExpressionAtlasi | Q08297, baseline and differential |
Genevisiblei | Q08297, MM |
Interactioni
Subunit structurei
Forms linear homooligomers, giving rise to a RAD51 nucleoprotein filament, which is essential for strand-pairing reactions during DNA recombination.
Interacts with BRCA1 and either directly or indirectly with p53.
Interacts with XRCC3, RAD54L and RAD54B.
Interacts with the BCDX2 subcomplex RAD51C:RAD51B.
Interacts directly with PALB2 which may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3.
Interacts with RAD51AP1 and RAD51AP2.
Interacts with CHEK1, and this may require prior phosphorylation of CHEK1 (By similarity).
Interacts with the MND1-PSMC3IP heterodimer (PubMed:15834424).
Found in a complex, at least composed of BLM, RAD51 and SPIDR; the complex formation is mediated by SPIDR.
Interacts with SPIDR; the interaction is direct and recruits RAD51 to DNA damage sites.
Interacts with FIGNL1 (via N-terminal one-half region); the interaction is direct.
Interacts with RAD51AP1 (via C-terminal region); the interaction is direct (By similarity).
Interacts with NABP2, RPA1, PALB2 and RAD51.
Interacts with SWI5/C9orf119, and at lower level with SFR1/MEIR5 (PubMed:20976249).
Interacts with hyperphosphorylated RPA2; this interaction is necessary for efficient recruitment to chromatin in response to DNA damage.
Interacts with SWSAP1; involved in homologous recombination repair.
Interacts with PARPBP, BRCA2 and RECQL5; these interactions interfere with the formation of the RAD51-DNA homologous recombination structure.
Interacts with POLQ; POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends.
Interacts with POLN (By similarity).
Interacts with FBH1 (PubMed:24108124).
Interacts with RFWD3 (By similarity).
Interacts with the MCM8-MCM9 complex; the interaction recruits RAD51 to DNA damage sites (By similarity).
Component of a multiprotein complex with MEIOB and SPATA22.
Interacts with the complex BRME1:HSF2BP:BRCA2 (PubMed:32345962, PubMed:30760716).
By similarity5 PublicationsGO - Molecular functioni
- DNA polymerase binding Source: MGI
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- protein C-terminus binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202564, 12 interactors |
ComplexPortali | CPX-972, BRCC ubiquitin ligase complex |
CORUMi | Q08297 |
IntActi | Q08297, 1 interactor |
MINTi | Q08297 |
STRINGi | 10090.ENSMUSP00000028795 |
Miscellaneous databases
RNActi | Q08297, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 48 – 77 | HhHAdd BLAST | 30 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 184 – 257 | Interaction with PALB2By similarityAdd BLAST | 74 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 245 – 260 | Nuclear export signal; masked by the interaction with BRCA2By similarityAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1433, Eukaryota |
GeneTreei | ENSGT00940000156157 |
HOGENOMi | CLU_041732_0_2_1 |
InParanoidi | Q08297 |
OMAi | GFHTIEA |
OrthoDBi | 877394at2759 |
PhylomeDBi | Q08297 |
TreeFami | TF101218 |
Family and domain databases
CDDi | cd01123, Rad51_DMC1_radA, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011941, DNA_recomb/repair_Rad51 IPR013632, DNA_recomb/repair_Rad51_C IPR016467, DNA_recomb/repair_RecA-like IPR010995, DNA_repair_Rad51/TF_NusA_a-hlx IPR027417, P-loop_NTPase IPR033925, Rad51_DMC1_RadA IPR020588, RecA_ATP-bd IPR020587, RecA_monomer-monomer_interface |
Pfami | View protein in Pfam PF08423, Rad51, 1 hit |
PIRSFi | PIRSF005856, Rad51, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF47794, SSF47794, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR02239, recomb_RAD51, 1 hit |
PROSITEi | View protein in PROSITE PS50162, RECA_2, 1 hit PS50163, RECA_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAMQMQLEAS ADTSVEEESF GPQPISRLEQ CGINANDVKK LEEAGYHTVE
60 70 80 90 100
AVAYAPKKEL INIKGISEAK ADKILTEAAK LVPMGFTTAT EFHQRRSEII
110 120 130 140 150
QITTGSKELD KLLQGGIETG SITEMFGEFR TGKTQICHTL AVTCQLPIDR
160 170 180 190 200
GGGEGKAMYI DTEGTFRPER LLAVAERYGL SGSDVLDNVA YARGFNTDHQ
210 220 230 240 250
TQLLYQASAM MVESRYALLI VDSATALYRT DYSGRGELSA RQMHLARFLR
260 270 280 290 300
MLLRLADEFG VAVVITNQVV AQVDGAAMFA ADPKKPIGGN IIAHASTTRL
310 320 330
YLRKGRGETR ICKIYDSPCL PEAEAMFAIN ADGVGDAKD
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RCK1 | D6RCK1_MOUSE | DNA repair protein RAD51 homolog 1 | Rad51 | 220 | Annotation score: | ||
F7AT35 | F7AT35_MOUSE | DNA repair protein RAD51 homolog 1 | Rad51 | 237 | Annotation score: | ||
A3KGI2 | A3KGI2_MOUSE | DNA repair protein RAD51 homolog 1 | Rad51 | 178 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13473 mRNA Translation: BAA02718.1 D13803 mRNA Translation: BAA02961.1 AK011242 mRNA Translation: BAB27489.1 AK076468 mRNA Translation: BAC36357.1 AK151157 mRNA Translation: BAE30162.1 AK151177 mRNA Translation: BAE30179.1 BC027384 mRNA Translation: AAH27384.1 |
CCDSi | CCDS16590.1 |
PIRi | A48221 |
RefSeqi | NP_035364.1, NM_011234.4 |
Genome annotation databases
Ensembli | ENSMUST00000028795; ENSMUSP00000028795; ENSMUSG00000027323 |
GeneIDi | 19361 |
KEGGi | mmu:19361 |
UCSCi | uc008ltd.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13473 mRNA Translation: BAA02718.1 D13803 mRNA Translation: BAA02961.1 AK011242 mRNA Translation: BAB27489.1 AK076468 mRNA Translation: BAC36357.1 AK151157 mRNA Translation: BAE30162.1 AK151177 mRNA Translation: BAE30179.1 BC027384 mRNA Translation: AAH27384.1 |
CCDSi | CCDS16590.1 |
PIRi | A48221 |
RefSeqi | NP_035364.1, NM_011234.4 |
3D structure databases
BMRBi | Q08297 |
SMRi | Q08297 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202564, 12 interactors |
ComplexPortali | CPX-972, BRCC ubiquitin ligase complex |
CORUMi | Q08297 |
IntActi | Q08297, 1 interactor |
MINTi | Q08297 |
STRINGi | 10090.ENSMUSP00000028795 |
Chemistry databases
ChEMBLi | CHEMBL2034808 |
PTM databases
iPTMneti | Q08297 |
PhosphoSitePlusi | Q08297 |
Proteomic databases
EPDi | Q08297 |
MaxQBi | Q08297 |
PaxDbi | Q08297 |
PRIDEi | Q08297 |
Protocols and materials databases
Antibodypediai | 4162, 911 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028795; ENSMUSP00000028795; ENSMUSG00000027323 |
GeneIDi | 19361 |
KEGGi | mmu:19361 |
UCSCi | uc008ltd.1, mouse |
Organism-specific databases
CTDi | 5888 |
MGIi | MGI:97890, Rad51 |
Phylogenomic databases
eggNOGi | KOG1433, Eukaryota |
GeneTreei | ENSGT00940000156157 |
HOGENOMi | CLU_041732_0_2_1 |
InParanoidi | Q08297 |
OMAi | GFHTIEA |
OrthoDBi | 877394at2759 |
PhylomeDBi | Q08297 |
TreeFami | TF101218 |
Enzyme and pathway databases
Reactomei | R-MMU-5685938, HDR through Single Strand Annealing (SSA) R-MMU-5685942, HDR through Homologous Recombination (HRR) R-MMU-5693568, Resolution of D-loop Structures through Holliday Junction Intermediates R-MMU-5693579, Homologous DNA Pairing and Strand Exchange R-MMU-5693616, Presynaptic phase of homologous DNA pairing and strand exchange |
Miscellaneous databases
BioGRID-ORCSi | 19361, 8 hits in 18 CRISPR screens |
ChiTaRSi | Xrcc2, mouse |
PROi | PR:Q08297 |
RNActi | Q08297, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027323, Expressed in ear vesicle and 237 other tissues |
ExpressionAtlasi | Q08297, baseline and differential |
Genevisiblei | Q08297, MM |
Family and domain databases
CDDi | cd01123, Rad51_DMC1_radA, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011941, DNA_recomb/repair_Rad51 IPR013632, DNA_recomb/repair_Rad51_C IPR016467, DNA_recomb/repair_RecA-like IPR010995, DNA_repair_Rad51/TF_NusA_a-hlx IPR027417, P-loop_NTPase IPR033925, Rad51_DMC1_RadA IPR020588, RecA_ATP-bd IPR020587, RecA_monomer-monomer_interface |
Pfami | View protein in Pfam PF08423, Rad51, 1 hit |
PIRSFi | PIRSF005856, Rad51, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF47794, SSF47794, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR02239, recomb_RAD51, 1 hit |
PROSITEi | View protein in PROSITE PS50162, RECA_2, 1 hit PS50163, RECA_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAD51_MOUSE | |
Accessioni | Q08297Primary (citable) accession number: Q08297 Secondary accession number(s): Q3UAY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 201 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families