UniProtKB - Q08273 (RBX1_YEAST)
RING-box protein HRT1
HRT1
Functioni
Caution
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 55 | Zinc 1By similarity | 1 | |
Metal bindingi | 58 | Zinc 1By similarity | 1 | |
Metal bindingi | 66 | Zinc 2By similarity | 1 | |
Metal bindingi | 69 | Zinc 2By similarity | 1 | |
Metal bindingi | 81 | Zinc 2By similarity | 1 | |
Metal bindingi | 88 | Zinc 3By similarity | 1 | |
Metal bindingi | 90 | Zinc 3; via pros nitrogenBy similarity | 1 | |
Metal bindingi | 93 | Zinc 1; via pros nitrogenBy similarity | 1 | |
Metal bindingi | 95 | Zinc 2By similarity | 1 | |
Metal bindingi | 107 | Zinc 3By similarity | 1 | |
Metal bindingi | 110 | Zinc 3By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 55 – 111 | RING-typePROSITE-ProRule annotationAdd BLAST | 57 |
GO - Molecular functioni
- cullin family protein binding Source: GO_Central
- protein-macromolecule adaptor activity Source: SGD
- ubiquitin protein ligase activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- cell division Source: UniProtKB-KW
- DNA repair Source: UniProtKB-KW
- G1/S transition of mitotic cell cycle Source: SGD
- protein ubiquitination Source: GO_Central
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
- ubiquitin-dependent protein catabolic process Source: SGD
Keywordsi
Biological process | Cell cycle, Cell division, DNA damage, DNA repair, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-6782135, Dual incision in TC-NER R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-SCE-8951664, Neddylation R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: RING-box protein HRT1Short name: RING-box protein 1 Alternative name(s): E3 ubiquitin-protein ligase complex SCF subunit HRT1 High level expression reduces Ty3 transposition protein 1 Regulator of cullins protein 1 |
Gene namesi | Name:HRT1 Synonyms:RBX1, ROC1 Ordered Locus Names:YOL133W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005493, HRT1 |
VEuPathDBi | FungiDB:YOL133W |
Subcellular locationi
Nucleus
- nucleus Source: SGD
Other locations
- Cul3-RING ubiquitin ligase complex Source: SGD
- Cul8-RING ubiquitin ligase complex Source: SGD
- cullin-RING ubiquitin ligase complex Source: GO_Central
- cytoplasm Source: SGD
- SCF ubiquitin ligase complex Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 72 | K → R in RBX1-1; temperature-sensitive allele. At 38 degrees Celsius induces defects in ubiquitin ligase activity; when associated with R-81. | 1 | |
Mutagenesisi | 81 | C → R in RBX1-1; temperature-sensitive allele. At 38 degrees Celsius induces defects in ubiquitin ligase activity; when associated with R-72. | 1 | |
Mutagenesisi | 81 | C → Y in HRT1-C81Y; defects in ubiquitin ligase activity. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056022 | 1 – 121 | RING-box protein HRT1Add BLAST | 121 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q08273 |
PaxDbi | Q08273 |
PRIDEi | Q08273 |
PTM databases
iPTMneti | Q08273 |
Interactioni
Subunit structurei
Component of multiple cullin-RING ligases (CRLs) composed of 4 subunits: the RING protein HRT1, a cullin, a linker protein, and one of many alternative substrate receptors.
Component of SCF E3 ubiquitin ligase complexes containing the cullin CDC53, the linker protein SKP1/CBF3D, and substrate receptors containing F-box motifs like DAS1 or GRR1.
Component of RTT101(MMS1) E3 ubiquitin ligase complexes containing the cullin RTT101, the linker protein MMS1, and substrate receptors belonging to a protein family described as DCAF (DDB1- and CUL4-associated factor) like MMS22.
Component of CRL3 E3 ubiquitin ligase complexes containing the cullin CUL3, the linker protein ELC1, and substrate receptors containing SOCS-box motifs like ELA1.
Interacts with CDC53, CUL3, RTT101, CDC4 and CDC34/UBC3.
5 PublicationsBinary interactionsi
Hide detailsQ08273
GO - Molecular functioni
- cullin family protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 34242, 94 interactors |
ComplexPortali | CPX-1157, CUL8-MMS1-MMS22-ESC4 E3 ubiquitin ligase complex CPX-1165, CUL8-MMS1-MMS22-CTF4 E3 ubiquitin ligase complex CPX-1166, CUL8-MMS1-ESC2 E3 ubiquitin ligase complex CPX-1167, CUL8-MMS1-ORC5 E3 ubiquitin ligase complex CPX-1837, CUL3-HRT1/ELC1/ELA1 ubiquitin ligase complex CPX-3234, SCF-Cdc4 ubiquitin ligase complex CPX-3241, SCF-Grr1 ubiquitin ligase complex CPX-3242, SCF-Mdm30 ubiquitin ligase complex CPX-3243, SCF-Ufo1 ubiquitin ligase complex CPX-3244, SCF-Das1 ubiquitin ligase complex CPX-3249, SCF-Met30 ubiquitin ligase complex CPX-3250, SCF-Dia2 ubiquitin ligase complex CPX-3253, SCF-Ylr352w ubiquitin ligase complex CPX-3254, SCF-Saf1 ubiquitin ligase complex CPX-3255, SCF-Hrt3 ubiquitin ligase complex CPX-3681, SCF-Ydr131c ubiquitin ligase complex |
DIPi | DIP-1373N |
IntActi | Q08273, 160 interactors |
MINTi | Q08273 |
STRINGi | 4932.YOL133W |
Miscellaneous databases
RNActi | Q08273, protein |
Family & Domainsi
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 55 – 111 | RING-typePROSITE-ProRule annotationAdd BLAST | 57 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2930, Eukaryota |
GeneTreei | ENSGT00940000168153 |
HOGENOMi | CLU_115512_2_1_1 |
InParanoidi | Q08273 |
OMAi | GHEYHTH |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR024766, Znf_RING_H2 |
Pfami | View protein in Pfam PF12678, zf-rbx1, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSNEVDRMDV DEDESQNIAQ SSNQSAPVET KKKRFEIKKW TAVAFWSWDI
60 70 80 90 100
AVDNCAICRN HIMEPCIECQ PKAMTDTDNE CVAAWGVCNH AFHLHCINKW
110 120
IKTRDACPLD NQPWQLARCG R
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X95465 Genomic DNA Translation: CAA64737.1 Z74876 Genomic DNA Translation: CAA99155.1 BK006948 Genomic DNA Translation: DAA10651.1 |
PIRi | S66830 |
RefSeqi | NP_014508.1, NM_001183387.1 |
Genome annotation databases
EnsemblFungii | YOL133W_mRNA; YOL133W; YOL133W |
GeneIDi | 853986 |
KEGGi | sce:YOL133W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X95465 Genomic DNA Translation: CAA64737.1 Z74876 Genomic DNA Translation: CAA99155.1 BK006948 Genomic DNA Translation: DAA10651.1 |
PIRi | S66830 |
RefSeqi | NP_014508.1, NM_001183387.1 |
3D structure databases
SMRi | Q08273 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34242, 94 interactors |
ComplexPortali | CPX-1157, CUL8-MMS1-MMS22-ESC4 E3 ubiquitin ligase complex CPX-1165, CUL8-MMS1-MMS22-CTF4 E3 ubiquitin ligase complex CPX-1166, CUL8-MMS1-ESC2 E3 ubiquitin ligase complex CPX-1167, CUL8-MMS1-ORC5 E3 ubiquitin ligase complex CPX-1837, CUL3-HRT1/ELC1/ELA1 ubiquitin ligase complex CPX-3234, SCF-Cdc4 ubiquitin ligase complex CPX-3241, SCF-Grr1 ubiquitin ligase complex CPX-3242, SCF-Mdm30 ubiquitin ligase complex CPX-3243, SCF-Ufo1 ubiquitin ligase complex CPX-3244, SCF-Das1 ubiquitin ligase complex CPX-3249, SCF-Met30 ubiquitin ligase complex CPX-3250, SCF-Dia2 ubiquitin ligase complex CPX-3253, SCF-Ylr352w ubiquitin ligase complex CPX-3254, SCF-Saf1 ubiquitin ligase complex CPX-3255, SCF-Hrt3 ubiquitin ligase complex CPX-3681, SCF-Ydr131c ubiquitin ligase complex |
DIPi | DIP-1373N |
IntActi | Q08273, 160 interactors |
MINTi | Q08273 |
STRINGi | 4932.YOL133W |
PTM databases
iPTMneti | Q08273 |
Proteomic databases
MaxQBi | Q08273 |
PaxDbi | Q08273 |
PRIDEi | Q08273 |
Genome annotation databases
EnsemblFungii | YOL133W_mRNA; YOL133W; YOL133W |
GeneIDi | 853986 |
KEGGi | sce:YOL133W |
Organism-specific databases
SGDi | S000005493, HRT1 |
VEuPathDBi | FungiDB:YOL133W |
Phylogenomic databases
eggNOGi | KOG2930, Eukaryota |
GeneTreei | ENSGT00940000168153 |
HOGENOMi | CLU_115512_2_1_1 |
InParanoidi | Q08273 |
OMAi | GHEYHTH |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-SCE-6782135, Dual incision in TC-NER R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-SCE-8951664, Neddylation R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:Q08273 |
RNActi | Q08273, protein |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR024766, Znf_RING_H2 |
Pfami | View protein in Pfam PF12678, zf-rbx1, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RBX1_YEAST | |
Accessioni | Q08273Primary (citable) accession number: Q08273 Secondary accession number(s): D6W1T5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2003 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names