UniProtKB - Q08189 (TGM3_MOUSE)
Protein
Protein-glutamine gamma-glutamyltransferase E
Gene
Tgm3
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.By similarity
Catalytic activityi
- L-glutaminyl-[protein] + L-lysyl-[protein] = [protein]-L-lysyl-N6-5-L-glutamyl-[protein] + NH4+PROSITE-ProRule annotationBy similarityEC:2.3.2.13PROSITE-ProRule annotationBy similarity
Cofactori
Ca2+By similarityNote: Binds 3 Ca2+ cations per subunit. Binds 1 Ca2+ as a zymogen, and binds 2 more Ca2+ cations, or other divalent metal cations, after proteolytic processing.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 222 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 225 | Calcium 1By similarity | 1 | |
Metal bindingi | 227 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 228 | Calcium 1By similarity | 1 | |
Metal bindingi | 230 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 273 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 302 | Calcium 2By similarity | 1 | |
Metal bindingi | 304 | Calcium 2By similarity | 1 | |
Metal bindingi | 306 | Calcium 2By similarity | 1 | |
Metal bindingi | 308 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 325 | Calcium 2By similarity | 1 | |
Active sitei | 331 | PROSITE-ProRule annotation | 1 | |
Active sitei | 354 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 394 | Calcium 3By similarity | 1 | |
Metal bindingi | 416 | Calcium 3; via carbonyl oxygen | 1 | |
Metal bindingi | 444 | Calcium 3By similarity | 1 | |
Metal bindingi | 449 | Calcium 3By similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- catalytic activity Source: UniProtKB
- protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB
- structural molecule activity Source: UniProtKB
GO - Biological processi
- cell envelope organization Source: UniProtKB
- epidermis development Source: UniProtKB
- hair cell differentiation Source: UniProtKB
- keratinization Source: UniProtKB-KW
- keratinocyte differentiation Source: UniProtKB
- peptide cross-linking Source: UniProtKB
- skin development Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Keratinization |
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-glutamine gamma-glutamyltransferase E (EC:2.3.2.13By similarity)Alternative name(s): Transglutaminase E Short name: TG(E) Short name: TGE Short name: TGase E Transglutaminase-3 Short name: TGase-3 Cleaved into the following 2 chains: |
Gene namesi | Name:Tgm3 Synonyms:Tgase3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98732, Tgm3 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
Extracellular region or secreted
- collagen-containing extracellular matrix Source: BHF-UCL
Plasma Membrane
- cornified envelope Source: UniProtKB
- extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- protein-containing complex Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000033654 | 1 – 467 | Protein-glutamine gamma-glutamyltransferase E 50 kDa catalytic chainAdd BLAST | 467 | |
ChainiPRO_0000033655 | 468 – 693 | Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chainAdd BLAST | 226 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 111 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 112 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 467 – 468 | Cleavage; by CTSLBy similarity | 2 |
Keywords - PTMi
Phosphoprotein, ZymogenProteomic databases
MaxQBi | Q08189 |
PaxDbi | Q08189 |
PeptideAtlasi | Q08189 |
PRIDEi | Q08189 |
PTM databases
iPTMneti | Q08189 |
PhosphoSitePlusi | Q08189 |
Expressioni
Tissue specificityi
Expressed in skin and stomach and, at lower levels, in testis, kidney and spleen (at protein level). On the basis of its catalytic activity, detected in the epidermis, around the granular and spinous layers but not in the outermost cornified layers. In hair follicles, mainly located in the medulla and the hair cortex.2 Publications
Developmental stagei
Expression starts at 11.5 dpc in the early two-layered epidermis. From 12.5 dpc, mainly expressed in the periderm cells and weakly in the epidermal basal cells. After epidermis keratinization, at 15.5 to 17.5 dpc, detected in the granular, cornified layers and in the hair follicle. Also expressed in heart, lung, bone, muscle, testis and blood vessels at 12.5, 13.5, 14.5 and 16.5 dpc, respectively.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000027401, Expressed in esophagus and 107 other tissues |
Genevisiblei | Q08189, MM |
Interactioni
Subunit structurei
Consists of two polypeptide chains, which are synthesized as a precursor form of a single polypeptide.
Protein-protein interaction databases
BioGRIDi | 204169, 8 interactors |
IntActi | Q08189, 3 interactors |
STRINGi | 10090.ENSMUSP00000105928 |
Miscellaneous databases
RNActi | Q08189, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QUPB, Eukaryota |
GeneTreei | ENSGT01010000222463 |
HOGENOMi | CLU_013435_1_0_1 |
InParanoidi | Q08189 |
OMAi | GWNFGQY |
OrthoDBi | 297055at2759 |
PhylomeDBi | Q08189 |
TreeFami | TF324278 |
Family and domain databases
Gene3Di | 2.60.40.10, 3 hits 3.90.260.10, 1 hit |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR038765, Papain-like_cys_pep_sf IPR002931, Transglutaminase-like IPR036985, Transglutaminase-like_sf IPR023608, Transglutaminase_animal IPR013808, Transglutaminase_AS IPR008958, Transglutaminase_C IPR036238, Transglutaminase_C_sf IPR001102, Transglutaminase_N |
Pfami | View protein in Pfam PF00927, Transglut_C, 2 hits PF01841, Transglut_core, 1 hit PF00868, Transglut_N, 1 hit |
PIRSFi | PIRSF000459, TGM_EBP42, 1 hit |
SMARTi | View protein in SMART SM00460, TGc, 1 hit |
SUPFAMi | SSF49309, SSF49309, 2 hits SSF54001, SSF54001, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS00547, TRANSGLUTAMINASES, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q08189-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSALQIQNVN WQVPMNRRAH HTDKFSSQDS IVRRGQPWEI ILVCNRSLES
60 70 80 90 100
GEDLNFIVST GPQPSESART KAVFSISGRS TGGWNAALKA NSGNNLAIAI
110 120 130 140 150
ASPVSAPIGL YTLSVEISSR GRASSLKLGT FIMLFNPWLQ ADDVFMSNHA
160 170 180 190 200
ERQEYVEEDS GIIYVGSTNR IGMVGWNFGQ FEEDILNISL SILDRSLNFR
210 220 230 240 250
RDPVTDVARR NDPKYVCRVL SAMINGNDDN GVISGNWSGN YTGGVDPRTW
260 270 280 290 300
NGSVEILKNW KKSGFRPVQF GQCWVFAGTL NTVLRCLGVP SRVITNFNSA
310 320 330 340 350
HDTDRNLSVD VYYDAMGNPL EKGSDSVWNF HVWNEGWFVR TDLGPTYNGW
360 370 380 390 400
QVLDATPQER SQGVFQCGPA SVNAIKAGDV DRNFDMIFIF AEVNADRITW
410 420 430 440 450
IYNNRNNTQK QNSVDTHSIG KYISTKAVGS NSRMDVTDKY KYPEGSSEER
460 470 480 490 500
QVHQKALDKL KPNASFGATS SRNPEGEDKE PSISGKFKVT GILAVGKEVS
510 520 530 540 550
LSLMLKNMTN DRKTVTMNMT AWTIVYNGTL VHEVWKDSAT ISLDPEEEIQ
560 570 580 590 600
YPVKIAYSQY ERYLKADNMI RITAVCKVPD EAEVVVERDV ILDNPALTLE
610 620 630 640 650
VLEQAHVRKP VNVQMLFSNP LDQPVNNCVL LVEGSGLLRG SLKIDVPSLR
660 670 680 690
PKEKSRIRFE IFPTRSGTKQ LLADFSCNKF PAIKAMLPID VSE
Sequence cautioni
The sequence AAA40421 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 290 | P → R in AAA40421 (PubMed:8099584).Curated | 1 | |
Sequence conflicti | 409 | Q → P in AAI29892 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 409 | Q → P in AAI29891 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 573 | T → S in AAA40421 (PubMed:8099584).Curated | 1 | |
Sequence conflicti | 588 | R → W in AAA40421 (PubMed:8099584).Curated | 1 | |
Sequence conflicti | 616 | L → I in AAA40421 (PubMed:8099584).Curated | 1 | |
Sequence conflicti | 639 | R → G in AAA40421 (PubMed:8099584).Curated | 1 | |
Sequence conflicti | 682 | A → T in AAA40421 (PubMed:8099584).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10385 mRNA Translation: AAA40421.1 Frameshift. AL808127 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL28248.1 BC129890 mRNA Translation: AAI29891.1 BC129891 mRNA Translation: AAI29892.1 |
CCDSi | CCDS38240.1 |
PIRi | B45991 |
RefSeqi | NP_033400.2, NM_009374.3 |
Genome annotation databases
Ensembli | ENSMUST00000110299; ENSMUSP00000105928; ENSMUSG00000027401 |
GeneIDi | 21818 |
KEGGi | mmu:21818 |
UCSCi | uc008mig.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10385 mRNA Translation: AAA40421.1 Frameshift. AL808127 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL28248.1 BC129890 mRNA Translation: AAI29891.1 BC129891 mRNA Translation: AAI29892.1 |
CCDSi | CCDS38240.1 |
PIRi | B45991 |
RefSeqi | NP_033400.2, NM_009374.3 |
3D structure databases
SMRi | Q08189 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204169, 8 interactors |
IntActi | Q08189, 3 interactors |
STRINGi | 10090.ENSMUSP00000105928 |
PTM databases
iPTMneti | Q08189 |
PhosphoSitePlusi | Q08189 |
Proteomic databases
MaxQBi | Q08189 |
PaxDbi | Q08189 |
PeptideAtlasi | Q08189 |
PRIDEi | Q08189 |
Genome annotation databases
Ensembli | ENSMUST00000110299; ENSMUSP00000105928; ENSMUSG00000027401 |
GeneIDi | 21818 |
KEGGi | mmu:21818 |
UCSCi | uc008mig.2, mouse |
Organism-specific databases
CTDi | 7053 |
MGIi | MGI:98732, Tgm3 |
Phylogenomic databases
eggNOGi | ENOG502QUPB, Eukaryota |
GeneTreei | ENSGT01010000222463 |
HOGENOMi | CLU_013435_1_0_1 |
InParanoidi | Q08189 |
OMAi | GWNFGQY |
OrthoDBi | 297055at2759 |
PhylomeDBi | Q08189 |
TreeFami | TF324278 |
Miscellaneous databases
BioGRID-ORCSi | 21818, 0 hits in 17 CRISPR screens |
ChiTaRSi | Tgm3, mouse |
PROi | PR:Q08189 |
RNActi | Q08189, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027401, Expressed in esophagus and 107 other tissues |
Genevisiblei | Q08189, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 3 hits 3.90.260.10, 1 hit |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR038765, Papain-like_cys_pep_sf IPR002931, Transglutaminase-like IPR036985, Transglutaminase-like_sf IPR023608, Transglutaminase_animal IPR013808, Transglutaminase_AS IPR008958, Transglutaminase_C IPR036238, Transglutaminase_C_sf IPR001102, Transglutaminase_N |
Pfami | View protein in Pfam PF00927, Transglut_C, 2 hits PF01841, Transglut_core, 1 hit PF00868, Transglut_N, 1 hit |
PIRSFi | PIRSF000459, TGM_EBP42, 1 hit |
SMARTi | View protein in SMART SM00460, TGc, 1 hit |
SUPFAMi | SSF49309, SSF49309, 2 hits SSF54001, SSF54001, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS00547, TRANSGLUTAMINASES, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TGM3_MOUSE | |
Accessioni | Q08189Primary (citable) accession number: Q08189 Secondary accession number(s): A1L343, A2ANC3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | May 31, 2011 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families