UniProtKB - Q08162 (RRP44_YEAST)
Exosome complex exonuclease DIS3
DIS3
Functioni
Catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. DIS3 has both 3'-5' exonuclease and endonuclease activities. The exonuclease activity of DIS3 is down-regulated upon association with Exo-9 possibly involving a conformational change in the catalytic domain and threading of the RNA substrate through the complex central channel. Structured substrates can be degraded if they have a 3' single-stranded extension sufficiently long (such as 35 nt poly(A)) to span the proposed complex inner RNA-binding path and to reach the exonuclease site provided by DIS3. Plays a role in mitotic control.
3 PublicationsMiscellaneous
Caution
Cofactori
GO - Molecular functioni
- 3'-5'-exoribonuclease activity Source: SGD
- endonuclease activity Source: GO_Central
- endoribonuclease activity Source: SGD
- tRNA binding Source: SGD
GO - Biological processi
- exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
- mRNA processing Source: SGD
- ncRNA 3'-end processing Source: SGD
- nonfunctional rRNA decay Source: SGD
- nuclear mRNA surveillance Source: SGD
- nuclear polyadenylation-dependent CUT catabolic process Source: SGD
- nuclear polyadenylation-dependent mRNA catabolic process Source: SGD
- nuclear polyadenylation-dependent rRNA catabolic process Source: SGD
- nuclear polyadenylation-dependent tRNA catabolic process Source: SGD
- nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
- nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
- polyadenylation-dependent snoRNA 3'-end processing Source: SGD
- RNA catabolic process Source: ComplexPortal
- RNA processing Source: ComplexPortal
- rRNA catabolic process Source: SGD
Keywordsi
Molecular function | Endonuclease, Exonuclease, Hydrolase, Nuclease, RNA-binding |
Biological process | rRNA processing |
Enzyme and pathway databases
BRENDAi | 3.1.13.1, 984 |
Reactomei | R-SCE-429958, mRNA decay by 3' to 5' exoribonuclease R-SCE-450385, Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA R-SCE-450513, Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DIS3 Synonyms:RRP44 Ordered Locus Names:YOL021C ORF Names:O2197 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005381, DIS3 |
VEuPathDBi | FungiDB:YOL021C |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Nucleus
- nucleolus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nuclear exosome (RNase complex) Source: SGD
- nucleolus Source: SGD
- nucleus Source: ComplexPortal
Other locations
- cytoplasmic exosome (RNase complex) Source: SGD
- exosome (RNase complex) Source: ComplexPortal
Keywords - Cellular componenti
Cytoplasm, Exosome, Mitochondrion, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 47 | C → S: Slow growth; when associated with S-52 and S-55. 1 Publication | 1 | |
Mutagenesisi | 52 | C → S: Slow growth; when associated with S-47 and S-55. 1 Publication | 1 | |
Mutagenesisi | 55 | C → S: Slow growth; when associated with S-47 and S-52. 1 Publication | 1 | |
Mutagenesisi | 171 | D → A: Abolishes endoribonucleolytic activity; no effect on growth. No growth; when associated with N-551. 1 Publication | 1 | |
Mutagenesisi | 198 | D → A: Abolishes endoribonucleolytic activity; no effect on growth. No growth; when associated with N-551. 1 Publication | 1 | |
Mutagenesisi | 551 | D → N: Exoribonucleolytic activity abolished. Accumulation of partially processed 5.8S rRNA and partially degraded 5' ETS. No growth; when associated with A-171. No growth; when associated with A-198. 3 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166423 | 1 – 1001 | Exosome complex exonuclease DIS3Add BLAST | 1001 |
Proteomic databases
MaxQBi | Q08162 |
PaxDbi | Q08162 |
PRIDEi | Q08162 |
Interactioni
Subunit structurei
Component of the RNA exosome complex. The catalytically inactive RNA exosome core (Exo-9) complex which is believed to associate with catalytic subunits DIS3 and RRP6 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric ring of RNase PH domain-containing subunits and peripheral S1 domain-containing components CSL4, RRP4 and RRP40 located on the top of the ring structure. DIS3 associates at the respective bottom side with Exo-9.
Interacts with GSP1.
7 PublicationsBinary interactionsi
Q08162
With | #Exp. | IntAct |
---|---|---|
RRP4 [P38792] | 5 | EBI-1740,EBI-1757 |
RRP40 [Q08285] | 3 | EBI-1740,EBI-1831 |
RRP46 [P53256] | 5 | EBI-1740,EBI-1842 |
SKI6 [P46948] | 9 | EBI-1740,EBI-1788 |
Protein-protein interaction databases
BioGRIDi | 34381, 240 interactors |
ComplexPortali | CPX-599, Nuclear/nucleolar exosome complex, DIS3-RRP6 variant CPX-603, Cytoplasmic exosome complex, DIS3 variant |
DIPi | DIP-2355N |
IntActi | Q08162, 24 interactors |
MINTi | Q08162 |
STRINGi | 4932.YOL021C |
Miscellaneous databases
RNActi | Q08162, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q08162 |
SMRi | Q08162 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q08162 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 904 – 1001 | S1 motifPROSITE-ProRule annotationAdd BLAST | 98 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 235 | EndoribonucleaseAdd BLAST | 235 | |
Regioni | 343 – 363 | DisorderedSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2102, Eukaryota |
GeneTreei | ENSGT00530000063106 |
HOGENOMi | CLU_002333_5_0_1 |
InParanoidi | Q08162 |
OMAi | VVKRNWR |
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit |
InterProi | View protein in InterPro IPR041505, Dis3_CSD2 IPR012340, NA-bd_OB-fold IPR029060, PIN-like_dom_sf IPR002716, PIN_dom IPR001900, RNase_II/R IPR022966, RNase_II/R_CS IPR033771, Rrp44_CSD1 IPR033770, RRP44_S1 IPR022967, S1_dom IPR003029, S1_domain |
Pfami | View protein in Pfam PF17849, OB_Dis3, 1 hit PF13638, PIN_4, 1 hit PF00773, RNB, 1 hit PF17216, Rrp44_CSD1, 1 hit PF17215, Rrp44_S1, 1 hit |
SMARTi | View protein in SMART SM00670, PINc, 1 hit SM00955, RNB, 1 hit SM00316, S1, 1 hit |
SUPFAMi | SSF50249, SSF50249, 4 hits SSF88723, SSF88723, 1 hit |
PROSITEi | View protein in PROSITE PS01175, RIBONUCLEASE_II, 1 hit PS50126, S1, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSVPAIAPRR KRLADGLSVT QKVFVRSRNG GATKIVREHY LRSDIPCLSR
60 70 80 90 100
SCTKCPQIVV PDAQNELPKF ILSDSPLELS APIGKHYVVL DTNVVLQAID
110 120 130 140 150
LLENPNCFFD VIVPQIVLDE VRNKSYPVYT RLRTLCRDSD DHKRFIVFHN
160 170 180 190 200
EFSEHTFVER LPNETINDRN DRAIRKTCQW YSEHLKPYDI NVVLVTNDRL
210 220 230 240 250
NREAATKEVE SNIITKSLVQ YIELLPNADD IRDSIPQMDS FDKDLERDTF
260 270 280 290 300
SDFTFPEYYS TARVMGGLKN GVLYQGNIQI SEYNFLEGSV SLPRFSKPVL
310 320 330 340 350
IVGQKNLNRA FNGDQVIVEL LPQSEWKAPS SIVLDSEHFD VNDNPDIEAG
360 370 380 390 400
DDDDNNESSS NTTVISDKQR RLLAKDAMIA QRSKKIQPTA KVVYIQRRSW
410 420 430 440 450
RQYVGQLAPS SVDPQSSSTQ NVFVILMDKC LPKVRIRTRR AAELLDKRIV
460 470 480 490 500
ISIDSWPTTH KYPLGHFVRD LGTIESAQAE TEALLLEHDV EYRPFSKKVL
510 520 530 540 550
ECLPAEGHDW KAPTKLDDPE AVSKDPLLTK RKDLRDKLIC SIDPPGCVDI
560 570 580 590 600
DDALHAKKLP NGNWEVGVHI ADVTHFVKPG TALDAEGAAR GTSVYLVDKR
610 620 630 640 650
IDMLPMLLGT DLCSLKPYVD RFAFSVIWEL DDSANIVNVN FMKSVIRSRE
660 670 680 690 700
AFSYEQAQLR IDDKTQNDEL TMGMRALLKL SVKLKQKRLE AGALNLASPE
710 720 730 740 750
VKVHMDSETS DPNEVEIKKL LATNSLVEEF MLLANISVAR KIYDAFPQTA
760 770 780 790 800
MLRRHAAPPS TNFEILNEML NTRKNMSISL ESSKALADSL DRCVDPEDPY
810 820 830 840 850
FNTLVRIMST RCMMAAQYFY SGAYSYPDFR HYGLAVDIYT HFTSPIRRYC
860 870 880 890 900
DVVAHRQLAG AIGYEPLSLT HRDKNKMDMI CRNINRKHRN AQFAGRASIE
910 920 930 940 950
YYVGQVMRNN ESTETGYVIK VFNNGIVVLV PKFGVEGLIR LDNLTEDPNS
960 970 980 990 1000
AAFDEVEYKL TFVPTNSDKP RDVYVFDKVE VQVRSVMDPI TSKRKAELLL
K
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D76430 Genomic DNA Translation: BAA11176.1 Z74763 Genomic DNA Translation: CAA99021.1 BK006948 Genomic DNA Translation: DAA10760.1 |
PIRi | S66704 |
RefSeqi | NP_014621.1, NM_001183275.1 |
Genome annotation databases
EnsemblFungii | YOL021C_mRNA; YOL021C; YOL021C |
GeneIDi | 854138 |
KEGGi | sce:YOL021C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D76430 Genomic DNA Translation: BAA11176.1 Z74763 Genomic DNA Translation: CAA99021.1 BK006948 Genomic DNA Translation: DAA10760.1 |
PIRi | S66704 |
RefSeqi | NP_014621.1, NM_001183275.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2VNU | X-ray | 2.30 | D | 242-1001 | [»] | |
2WP8 | X-ray | 3.00 | J | 25-1001 | [»] | |
4IFD | X-ray | 2.80 | J | 1-1001 | [»] | |
5C0W | X-ray | 4.60 | J | 1-1001 | [»] | |
5C0X | X-ray | 3.81 | J | 1-1001 | [»] | |
5G06 | electron microscopy | 4.20 | J | 1-1001 | [»] | |
5JEA | X-ray | 2.65 | J | 1-1001 | [»] | |
5K36 | X-ray | 3.10 | K | 1-1001 | [»] | |
5VZJ | X-ray | 3.30 | K | 1-1001 | [»] | |
6FSZ | electron microscopy | 4.60 | JJ | 1-1001 | [»] | |
6LQS | electron microscopy | 3.80 | R4 | 1-1001 | [»] | |
7AJT | electron microscopy | 4.60 | EK | 1-1001 | [»] | |
7AJU | electron microscopy | 3.80 | EK | 1-1001 | [»] | |
7D4I | electron microscopy | 4.00 | R4 | 1-1001 | [»] | |
AlphaFoldDBi | Q08162 | |||||
SMRi | Q08162 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34381, 240 interactors |
ComplexPortali | CPX-599, Nuclear/nucleolar exosome complex, DIS3-RRP6 variant CPX-603, Cytoplasmic exosome complex, DIS3 variant |
DIPi | DIP-2355N |
IntActi | Q08162, 24 interactors |
MINTi | Q08162 |
STRINGi | 4932.YOL021C |
Proteomic databases
MaxQBi | Q08162 |
PaxDbi | Q08162 |
PRIDEi | Q08162 |
Genome annotation databases
EnsemblFungii | YOL021C_mRNA; YOL021C; YOL021C |
GeneIDi | 854138 |
KEGGi | sce:YOL021C |
Organism-specific databases
SGDi | S000005381, DIS3 |
VEuPathDBi | FungiDB:YOL021C |
Phylogenomic databases
eggNOGi | KOG2102, Eukaryota |
GeneTreei | ENSGT00530000063106 |
HOGENOMi | CLU_002333_5_0_1 |
InParanoidi | Q08162 |
OMAi | VVKRNWR |
Enzyme and pathway databases
BRENDAi | 3.1.13.1, 984 |
Reactomei | R-SCE-429958, mRNA decay by 3' to 5' exoribonuclease R-SCE-450385, Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA R-SCE-450513, Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA |
Miscellaneous databases
EvolutionaryTracei | Q08162 |
PROi | PR:Q08162 |
RNActi | Q08162, protein |
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit |
InterProi | View protein in InterPro IPR041505, Dis3_CSD2 IPR012340, NA-bd_OB-fold IPR029060, PIN-like_dom_sf IPR002716, PIN_dom IPR001900, RNase_II/R IPR022966, RNase_II/R_CS IPR033771, Rrp44_CSD1 IPR033770, RRP44_S1 IPR022967, S1_dom IPR003029, S1_domain |
Pfami | View protein in Pfam PF17849, OB_Dis3, 1 hit PF13638, PIN_4, 1 hit PF00773, RNB, 1 hit PF17216, Rrp44_CSD1, 1 hit PF17215, Rrp44_S1, 1 hit |
SMARTi | View protein in SMART SM00670, PINc, 1 hit SM00955, RNB, 1 hit SM00316, S1, 1 hit |
SUPFAMi | SSF50249, SSF50249, 4 hits SSF88723, SSF88723, 1 hit |
PROSITEi | View protein in PROSITE PS01175, RIBONUCLEASE_II, 1 hit PS50126, S1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RRP44_YEAST | |
Accessioni | Q08162Primary (citable) accession number: Q08162 Secondary accession number(s): D6W244 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families