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Protein

Transducin-like enhancer protein 3

Gene

Tle3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity). May play an important role during spermatogenesis.By similarity

GO - Molecular functioni

  • repressing transcription factor binding Source: GO_Central
  • transcription corepressor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processDifferentiation, Spermatogenesis, Transcription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-201722 Formation of the beta-catenin:TCF transactivating complex
R-MMU-3769402 Deactivation of the beta-catenin transactivating complex
R-MMU-4641265 Repression of WNT target genes
R-MMU-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Transducin-like enhancer protein 3
Alternative name(s):
ESG
Grg-3
Gene namesi
Name:Tle3
Synonyms:Esg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:104634 Tle3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512811 – 772Transducin-like enhancer protein 3Add BLAST772

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei203PhosphoserineCombined sources1
Modified residuei207PhosphoserineBy similarity1
Modified residuei211PhosphoserineBy similarity1
Modified residuei217PhosphoserineBy similarity1
Modified residuei232N6-acetyllysineBy similarity1
Modified residuei240PhosphoserineBy similarity1
Modified residuei245PhosphoserineBy similarity1
Modified residuei259PhosphothreonineCombined sources1
Modified residuei263PhosphoserineCombined sources1
Modified residuei267PhosphoserineCombined sources1
Modified residuei275N6-acetyllysineBy similarity1
Modified residuei286PhosphoserineCombined sources1
Modified residuei312PhosphothreonineBy similarity1
Modified residuei317PhosphoserineBy similarity1
Modified residuei319PhosphothreonineBy similarity1
Modified residuei321PhosphothreonineBy similarity1
Modified residuei328PhosphothreonineCombined sources1
Modified residuei334PhosphothreonineCombined sources1
Modified residuei413PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ08122
PRIDEiQ08122

PTM databases

iPTMnetiQ08122
PhosphoSitePlusiQ08122

Expressioni

Tissue specificityi

Expressed only in testis.

Gene expression databases

BgeeiENSMUSG00000032280 Expressed in 367 organ(s), highest expression level in neocortex
ExpressionAtlasiQ08122 baseline and differential
GenevisibleiQ08122 MM

Interactioni

Subunit structurei

Homotetramer and heterooligomer with other family members. Binds FOXA2 (By similarity). Interacts with XIAP/BIRC4 (By similarity). Binds LEF1, TCF7, TCF7L1 and TCF7L2/TCF4. Interacts with TBX18 (via engrailed homology 1 repressor motif), leading to decreased of TBX18 transcriptional activity.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204218, 1 interactor
CORUMiQ08122
STRINGi10090.ENSMUSP00000124131

Structurei

3D structure databases

ProteinModelPortaliQ08122
SMRiQ08122
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati484 – 522WD 1Add BLAST39
Repeati530 – 569WD 2Add BLAST40
Repeati574 – 613WD 3Add BLAST40
Repeati616 – 655WD 4Add BLAST40
Repeati657 – 696WD 5Add BLAST40
Repeati698 – 737WD 6Add BLAST40
Repeati739 – 771WD 7Add BLAST33

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 131Q domainBy similarityAdd BLAST131
Regioni132 – 199GP domainBy similarityAdd BLAST68
Regioni200 – 268CcN domainBy similarityAdd BLAST69
Regioni269 – 452SP domainBy similarityAdd BLAST184

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi225 – 228Nuclear localization signalSequence analysis4

Domaini

WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.1 Publication

Sequence similaritiesi

Belongs to the WD repeat Groucho/TLE family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0639 Eukaryota
ENOG410XPX3 LUCA
GeneTreeiENSGT00550000074465
HOVERGENiHBG004689
InParanoidiQ08122
TreeFamiTF314167

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR005617 Groucho/TLE_N
IPR009146 Groucho_enhance
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR10814 PTHR10814, 1 hit
PfamiView protein in Pfam
PF03920 TLE_N, 1 hit
PF00400 WD40, 2 hits
PRINTSiPR01850 GROUCHOFAMLY
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 2 hits

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 12 potential isoforms that are computationally mapped.Show allAlign All

Isoform 3 (identifier: Q08122-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MYPQGRHPAP HQPGQPGFKF TVAESCDRIK DEFQFLQAQY HSLKVEYDKL
60 70 80 90 100
ANEKTEMQRH YVMYYEMSYG LNIEMHKQTE IAKRLNTILA QIMPFLSQEH
110 120 130 140 150
QQQVAQAVER AKQVTMTELN AIIGQQQLQA QHLSHATHGP PVQLPPHPSG
160 170 180 190 200
LQPPGIPPVT GSSSGLLALG ALGSQAHLAV KDEKNHHELD HRERESSTNN
210 220 230 240 250
SVSPSESLRA SEKHRGSADY SMEAKKRKAE EKDSLSRYDS DGDKSDDLVV
260 270 280 290 300
DVSNEDPATP RVSPAHSPPE NGLDKARGLK KDAPTSPASV ASSSSTPSSK
310 320 330 340 350
TKDLGHNDKS STPGLKSNTP TPRNDAPTPG TSTTPGLRSM PGKPPGMDPI
360 370 380 390 400
GIMASALRTP ITLTSSYPAP FAMMSHHEMN GSLTSPSAYA GLHNIPSQMS
410 420 430 440 450
AAAAAAAAAY GRSPMVSFGA VGFDPHPPMR ATGLPSSLAS IPGGKPAYSF
460 470 480 490 500
HVSADGQMQP VPFPHDALAG PGIPRHARQI NTLSHGEVVC AVTISNPTRH
510 520 530 540 550
VYTGGKGCVK IWDISQPGSK SPISQLDCLN RDNYIRSCKL LPDGRTLIVG
560 570 580 590 600
GEASTLTIWD LASPTPRIKA ELTSSAPACY ALAISPDAKV CFSCCSDGNI
610 620 630 640 650
AVWDLHNQTL VRQFQGHTDG ASCIDISHDG TKLWTGGLDN TVRSWDLREG
660 670 680 690 700
RQLQQHDFTS QIFSLGYCPT GEWLAVGMES SNVEVLHHTK PDKYQLHLHE
710 720 730 740 750
SCVLSLKFAY CGKWFVSTGK DNLLNAWRTP YGASIFQSKE SSSVLSCDIS
760 770
ADDKYIVTGS GDKKATVYEV IY
Length:772
Mass (Da):83,447
Last modified:November 24, 2009 - v3
Checksum:i39735F2718A8D27A
GO
Isoform 1 (identifier: Q08122-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-8: YPQGRHP → CPCGLQ

Show »
Length:771
Mass (Da):83,212
Checksum:iF8263B8A418F757A
GO
Isoform 2 (identifier: Q08122-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     351-353: Missing.

Note: No experimental confirmation available.
Show »
Length:769
Mass (Da):83,145
Checksum:i5AF18B9723D42E6C
GO
Isoform 4 (identifier: Q08122-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     124-124: G → GVRGLPNLPLT

Note: No experimental confirmation available.
Show »
Length:782
Mass (Da):84,508
Checksum:iEEE3F366B5229276
GO

Computationally mapped potential isoform sequencesi

There are 12 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WIE2F8WIE2_MOUSE
Transducin-like enhancer protein 3
Tle3
764Annotation score:
E0CXS9E0CXS9_MOUSE
Transducin-like enhancer protein 3
Tle3
767Annotation score:
J3KMT7J3KMT7_MOUSE
Transducin-like enhancer protein 3
Tle3
783Annotation score:
E0CXS2E0CXS2_MOUSE
Transducin-like enhancer protein 3
Tle3
760Annotation score:
E0CY14E0CY14_MOUSE
Transducin-like enhancer protein 3
Tle3
762Annotation score:
E0CXU1E0CXU1_MOUSE
Transducin-like enhancer protein 3
Tle3
716Annotation score:
F6TS96F6TS96_MOUSE
Transducin-like enhancer protein 3
Tle3
702Annotation score:
Q3TY99Q3TY99_MOUSE
Transducin-like enhancer protein 3
Tle3
712Annotation score:
E0CY76E0CY76_MOUSE
Transducin-like enhancer protein 3
Tle3
335Annotation score:
E0CYM5E0CYM5_MOUSE
Transducin-like enhancer protein 3
Tle3
235Annotation score:
There are more potential isoformsShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti545R → G in CAA51770 (PubMed:8365415).Curated1
Sequence conflicti559W → R in BAC27347 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0384412 – 8YPQGRHP → CPCGLQ in isoform 1. 2 Publications7
Alternative sequenceiVSP_038442124G → GVRGLPNLPLT in isoform 4. 1 Publication1
Alternative sequenceiVSP_007025351 – 353Missing in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73360 mRNA Translation: CAA51770.1
AC126672 Genomic DNA No translation available.
BC006672 mRNA Translation: AAH06672.1
BC056465 mRNA Translation: AAH56465.1
AK031322 mRNA Translation: BAC27347.1
CCDSiCCDS52825.1 [Q08122-3]
CCDS52826.1 [Q08122-4]
PIRiS35681
RefSeqiNP_001077396.1, NM_001083927.1 [Q08122-4]
NP_033415.2, NM_009389.2 [Q08122-3]
XP_006511091.1, XM_006511028.3 [Q08122-2]
UniGeneiMm.24255

Genome annotation databases

EnsembliENSMUST00000160882; ENSMUSP00000124131; ENSMUSG00000032280 [Q08122-4]
ENSMUST00000162583; ENSMUSP00000124977; ENSMUSG00000032280 [Q08122-2]
ENSMUST00000162973; ENSMUSP00000124173; ENSMUSG00000032280 [Q08122-3]
GeneIDi21887
KEGGimmu:21887
UCSCiuc009pzo.1 mouse [Q08122-4]
uc009pzp.1 mouse [Q08122-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73360 mRNA Translation: CAA51770.1
AC126672 Genomic DNA No translation available.
BC006672 mRNA Translation: AAH06672.1
BC056465 mRNA Translation: AAH56465.1
AK031322 mRNA Translation: BAC27347.1
CCDSiCCDS52825.1 [Q08122-3]
CCDS52826.1 [Q08122-4]
PIRiS35681
RefSeqiNP_001077396.1, NM_001083927.1 [Q08122-4]
NP_033415.2, NM_009389.2 [Q08122-3]
XP_006511091.1, XM_006511028.3 [Q08122-2]
UniGeneiMm.24255

3D structure databases

ProteinModelPortaliQ08122
SMRiQ08122
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204218, 1 interactor
CORUMiQ08122
STRINGi10090.ENSMUSP00000124131

PTM databases

iPTMnetiQ08122
PhosphoSitePlusiQ08122

Proteomic databases

PaxDbiQ08122
PRIDEiQ08122

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000160882; ENSMUSP00000124131; ENSMUSG00000032280 [Q08122-4]
ENSMUST00000162583; ENSMUSP00000124977; ENSMUSG00000032280 [Q08122-2]
ENSMUST00000162973; ENSMUSP00000124173; ENSMUSG00000032280 [Q08122-3]
GeneIDi21887
KEGGimmu:21887
UCSCiuc009pzo.1 mouse [Q08122-4]
uc009pzp.1 mouse [Q08122-3]

Organism-specific databases

CTDi7090
MGIiMGI:104634 Tle3

Phylogenomic databases

eggNOGiKOG0639 Eukaryota
ENOG410XPX3 LUCA
GeneTreeiENSGT00550000074465
HOVERGENiHBG004689
InParanoidiQ08122
TreeFamiTF314167

Enzyme and pathway databases

ReactomeiR-MMU-201722 Formation of the beta-catenin:TCF transactivating complex
R-MMU-3769402 Deactivation of the beta-catenin transactivating complex
R-MMU-4641265 Repression of WNT target genes
R-MMU-9018519 Estrogen-dependent gene expression

Miscellaneous databases

PROiPR:Q08122
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032280 Expressed in 367 organ(s), highest expression level in neocortex
ExpressionAtlasiQ08122 baseline and differential
GenevisibleiQ08122 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR005617 Groucho/TLE_N
IPR009146 Groucho_enhance
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR10814 PTHR10814, 1 hit
PfamiView protein in Pfam
PF03920 TLE_N, 1 hit
PF00400 WD40, 2 hits
PRINTSiPR01850 GROUCHOFAMLY
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTLE3_MOUSE
AccessioniPrimary (citable) accession number: Q08122
Secondary accession number(s): Q6PHP0, Q923A4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 24, 2009
Last modified: November 7, 2018
This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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