UniProtKB - Q08109 (HRD1_YEAST)
ERAD-associated E3 ubiquitin-protein ligase HRD1
HRD1
Functioni
Miscellaneous
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 349 – 400 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 52 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: ParkinsonsUK-UCL
- ubiquitin-protein transferase activity Source: SGD
GO - Biological processi
- endoplasmic reticulum unfolded protein response Source: SGD
- fungal-type cell wall organization Source: SGD
- protein autoubiquitination Source: SGD
- protein K48-linked ubiquitination Source: ParkinsonsUK-UCL
- retrograde protein transport, ER to cytosol Source: SGD
- ubiquitin-dependent ERAD pathway Source: ParkinsonsUK-UCL
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Protein family/group databases
TCDBi | 3.A.16.1.2, the endoplasmic reticular retrotranslocon (er-rt) family |
Names & Taxonomyi
Protein namesi | Recommended name: ERAD-associated E3 ubiquitin-protein ligase HRD1 (EC:2.3.2.27)Alternative name(s): HMG-CoA reductase degradation protein 1 RING-type E3 ubiquitin transferase HRD1Curated |
Gene namesi | Name:HRD1 Synonyms:DER3 Ordered Locus Names:YOL013C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005373, HRD1 |
VEuPathDBi | FungiDB:YOL013C |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: SGD
- Hrd1p ubiquitin ligase ERAD-L complex Source: SGD
- Hrd1p ubiquitin ligase ERAD-M complex Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | Cytoplasmic | 8 | |
Transmembranei | 9 – 29 | Helical; Name=1Add BLAST | 21 | |
Topological domaini | 30 – 45 | LumenalAdd BLAST | 16 | |
Transmembranei | 46 – 66 | Helical; Name=2Add BLAST | 21 | |
Topological domaini | 67 – 82 | CytoplasmicAdd BLAST | 16 | |
Transmembranei | 83 – 103 | Helical; Name=3Add BLAST | 21 | |
Topological domaini | 104 | Lumenal | 1 | |
Transmembranei | 105 – 125 | Helical; Name=4Add BLAST | 21 | |
Topological domaini | 126 – 144 | CytoplasmicAdd BLAST | 19 | |
Transmembranei | 145 – 165 | Helical; Name=5Add BLAST | 21 | |
Topological domaini | 166 – 184 | LumenalAdd BLAST | 19 | |
Transmembranei | 185 – 205 | Helical; Name=6Add BLAST | 21 | |
Topological domaini | 206 – 551 | CytoplasmicAdd BLAST | 346 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 399 | C → S: Stabilizes HRD1. Reduced interaction with substrate. Formation of oligomer with or without USA1. 5 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000240367 | 1 – 551 | ERAD-associated E3 ubiquitin-protein ligase HRD1Add BLAST | 551 |
Proteomic databases
MaxQBi | Q08109 |
PaxDbi | Q08109 |
PRIDEi | Q08109 |
PTM databases
iPTMneti | Q08109 |
Interactioni
Subunit structurei
Forms homooligomers in a USA1-dependent manner: HRD1 oligomerization appears to be important for ERAD-L function but less important for ERAD-M function, although the underlying molecular mechanisms are unclear.
Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factor YOS9, and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1.
Interacts with HRD3 and DER1. The complex interacts with the ERAD substrates HMG1 and HMG2.
Interacts with the associated E2 ubiquitin conjugating enzymes UBC1 and UBC7 with its membrane anchor CUE1.
7 PublicationsBinary interactionsi
Hide detailsQ08109
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 34391, 161 interactors |
ComplexPortali | CPX-3070, HRD1 ubiquitin ligase complex |
DIPi | DIP-8850N |
IntActi | Q08109, 12 interactors |
MINTi | Q08109 |
STRINGi | 4932.YOL013C |
Miscellaneous databases
RNActi | Q08109, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 517 – 551 | Interaction with USA1Add BLAST | 35 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 413 – 483 | Thr-richAdd BLAST | 71 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 349 – 400 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 52 |
Keywords - Domaini
Transmembrane, Transmembrane helix, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0802, Eukaryota |
GeneTreei | ENSGT00940000172216 |
HOGENOMi | CLU_026577_0_0_1 |
InParanoidi | Q08109 |
OMAi | KNWMERS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR032832, E3_lig_synoviolin/Hrd1 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR22763:SF169, PTHR22763:SF169, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MVPENRRKQL AIFVVVTYLL TFYCVYSATK TSVSFLQVTL KLNEGFNLMV
60 70 80 90 100
LSIFILLNST LLWQLLTKLL FGELRLIEHE HIFERLPFTI INTLFMSSLF
110 120 130 140 150
HERYFFTVAF FGLLLLYLKV FHWILKDRLE ALLQSINDST TMKTLIFSRF
160 170 180 190 200
SFNLVLLAVV DYQIITRCIS SIYTNQKSDI ESTSLYLIQV MEFTMLLIDL
210 220 230 240 250
LNLFLQTCLN FWEFYRSQQS LSNENNHIVH GDPTDENTVE SDQSQPVLND
260 270 280 290 300
DDDDDDDDRQ FTGLEGKFMY EKAIDVFTRF LKTALHLSML IPFRMPMMLL
310 320 330 340 350
KDVVWDILAL YQSGTSLWKI WRNNKQLDDT LVTVTVEQLQ NSANDDNICI
360 370 380 390 400
ICMDELIHSP NQQTWKNKNK KPKRLPCGHI LHLSCLKNWM ERSQTCPICR
410 420 430 440 450
LPVFDEKGNV VQTTFTSNSD ITTQTTVTDS TGIATDQQGF ANEVDLLPTR
460 470 480 490 500
TTSPDIRIVP TQNIDTLAMR TRSTSTPSPT WYTFPLHKTG DNSVGSSRSA
510 520 530 540 550
YEFLITNSDE KENGIPVKLT IENHEVNSLH GDGGEQIAKK IVIPDKFIQH
I
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z74755 Genomic DNA Translation: CAA99012.1 BK006948 Genomic DNA Translation: DAA10770.1 |
PIRi | S66695 |
RefSeqi | NP_014630.1, NM_001183267.1 |
Genome annotation databases
EnsemblFungii | YOL013C_mRNA; YOL013C; YOL013C |
GeneIDi | 854149 |
KEGGi | sce:YOL013C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z74755 Genomic DNA Translation: CAA99012.1 BK006948 Genomic DNA Translation: DAA10770.1 |
PIRi | S66695 |
RefSeqi | NP_014630.1, NM_001183267.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5V6P | electron microscopy | 4.10 | A/B | 1-407 | [»] | |
6VJY | electron microscopy | 4.30 | B | 1-430 | [»] | |
6VJZ | electron microscopy | 4.30 | B | 1-480 | [»] | |
6VK0 | electron microscopy | 4.10 | B | 1-480 | [»] | |
6VK1 | electron microscopy | 3.90 | B | 1-480 | [»] | |
SMRi | Q08109 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34391, 161 interactors |
ComplexPortali | CPX-3070, HRD1 ubiquitin ligase complex |
DIPi | DIP-8850N |
IntActi | Q08109, 12 interactors |
MINTi | Q08109 |
STRINGi | 4932.YOL013C |
Protein family/group databases
TCDBi | 3.A.16.1.2, the endoplasmic reticular retrotranslocon (er-rt) family |
PTM databases
iPTMneti | Q08109 |
Proteomic databases
MaxQBi | Q08109 |
PaxDbi | Q08109 |
PRIDEi | Q08109 |
Genome annotation databases
EnsemblFungii | YOL013C_mRNA; YOL013C; YOL013C |
GeneIDi | 854149 |
KEGGi | sce:YOL013C |
Organism-specific databases
SGDi | S000005373, HRD1 |
VEuPathDBi | FungiDB:YOL013C |
Phylogenomic databases
eggNOGi | KOG0802, Eukaryota |
GeneTreei | ENSGT00940000172216 |
HOGENOMi | CLU_026577_0_0_1 |
InParanoidi | Q08109 |
OMAi | KNWMERS |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:Q08109 |
RNActi | Q08109, protein |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR032832, E3_lig_synoviolin/Hrd1 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR22763:SF169, PTHR22763:SF169, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HRD1_YEAST | |
Accessioni | Q08109Primary (citable) accession number: Q08109 Secondary accession number(s): D6W254 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names